LOCUS       NM_001142640           10391 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens trinucleotide repeat containing adaptor 6C (TNRC6C),
            transcript variant 1, mRNA.
ACCESSION   NM_001142640
VERSION     NM_001142640.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Rhine,C.L., Neil,C., Wang,J., Maguire,S., Buerer,L., Salomon,M.,
            Meremikwu,I.C., Kim,J., Strande,N.T. and Fairbrother,W.G.
  TITLE     Massively parallel reporter assays discover de novo exonic splicing
            mutants in paralogs of Autism genes
  JOURNAL   PLoS Genet 18 (1), e1009884 (2022)
   PUBMED   35051175
  REMARK    GeneRIF: Massively parallel reporter assays discover de novo exonic
            splicing mutants in paralogs of Autism genes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10391)
  AUTHORS   Johnson,S.T., Chu,Y., Liu,J. and Corey,D.R.
  TITLE     Impact of scaffolding protein TNRC6 paralogs on gene expression and
            splicing
  JOURNAL   RNA 27 (9), 1004-1016 (2021)
   PUBMED   34108231
  REMARK    GeneRIF: Impact of scaffolding protein TNRC6 paralogs on gene
            expression and splicing.
REFERENCE   4  (bases 1 to 10391)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 10391)
  AUTHORS   Liu,Z., Johnson,S.T., Zhang,Z. and Corey,D.R.
  TITLE     Expression of TNRC6 (GW182) Proteins Is Not Necessary for Gene
            Silencing by Fully Complementary RNA Duplexes
  JOURNAL   Nucleic Acid Ther 29 (6), 323-334 (2019)
   PUBMED   31670606
  REMARK    GeneRIF: Expression of TNRC6 (GW182) Proteins Is Not Necessary for
            Gene Silencing by Fully Complementary RNA Duplexes.
REFERENCE   6  (bases 1 to 10391)
  AUTHORS   Baillat,D. and Shiekhattar,R.
  TITLE     Functional dissection of the human TNRC6 (GW182-related) family of
            proteins
  JOURNAL   Mol Cell Biol 29 (15), 4144-4155 (2009)
   PUBMED   19470757
REFERENCE   7  (bases 1 to 10391)
  AUTHORS   Lazzaretti,D., Tournier,I. and Izaurralde,E.
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence
            bound transcripts independently of Argonaute proteins
  JOURNAL   RNA 15 (6), 1059-1066 (2009)
   PUBMED   19383768
  REMARK    GeneRIF: Our findings indicate that TNRC6C, is recruited to miRNA
            targets through an interaction between their N-terminal domain and
            an Argonaute protein
REFERENCE   8  (bases 1 to 10391)
  AUTHORS   Zipprich,J.T., Bhattacharyya,S., Mathys,H. and Filipowicz,W.
  TITLE     Importance of the C-terminal domain of the human GW182 protein
            TNRC6C for translational repression
  JOURNAL   RNA 15 (5), 781-793 (2009)
   PUBMED   19304925
  REMARK    GeneRIF: Through deletion and mutagenesis, study identified the
            C-terminal part of TNRC6C encompassing the RRM RNA-binding motif as
            a key effector domain mediating protein synthesis repression by
            TNRC6C.
REFERENCE   9  (bases 1 to 10391)
  AUTHORS   Schneider,M.D., Najand,N., Chaker,S., Pare,J.M., Haskins,J.,
            Hughes,S.C., Hobman,T.C., Locke,J. and Simmonds,A.J.
  TITLE     Gawky is a component of cytoplasmic mRNA processing bodies required
            for early Drosophila development
  JOURNAL   J Cell Biol 174 (3), 349-358 (2006)
   PUBMED   16880270
REFERENCE   10 (bases 1 to 10391)
  AUTHORS   Zody,M.C., Garber,M., Adams,D.J., Sharpe,T., Harrow,J.,
            Lupski,J.R., Nicholson,C., Searle,S.M., Wilming,L., Young,S.K.,
            Abouelleil,A., Allen,N.R., Bi,W., Bloom,T., Borowsky,M.L.,
            Bugalter,B.E., Butler,J., Chang,J.L., Chen,C.K., Cook,A., Corum,B.,
            Cuomo,C.A., de Jong,P.J., DeCaprio,D., Dewar,K., FitzGerald,M.,
            Gilbert,J., Gibson,R., Gnerre,S., Goldstein,S., Grafham,D.V.,
            Grocock,R., Hafez,N., Hagopian,D.S., Hart,E., Norman,C.H.,
            Humphray,S., Jaffe,D.B., Jones,M., Kamal,M., Khodiyar,V.K.,
            LaButti,K., Laird,G., Lehoczky,J., Liu,X., Lokyitsang,T.,
            Loveland,J., Lui,A., Macdonald,P., Major,J.E., Matthews,L.,
            Mauceli,E., McCarroll,S.A., Mihalev,A.H., Mudge,J., Nguyen,C.,
            Nicol,R., O'Leary,S.B., Osoegawa,K., Schwartz,D.C., Shaw-Smith,C.,
            Stankiewicz,P., Steward,C., Swarbreck,D., Venkataraman,V.,
            Whittaker,C.A., Yang,X., Zimmer,A.R., Bradley,A., Hubbard,T.,
            Birren,B.W., Rogers,J., Lander,E.S. and Nusbaum,C.
  TITLE     DNA sequence of human chromosome 17 and analysis of rearrangement
            in the human lineage
  JOURNAL   Nature 440 (7087), 1045-1049 (2006)
   PUBMED   16625196
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC015804.14 and AC021593.15.
            
            On Jul 14, 2021 this sequence version replaced NM_001142640.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2148874,
                              SAMN03465418 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000696270.1/ ENSP00000512514.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               AC015804.14        140214-140779
            567-666             AC015804.14        185680-185779
            667-688             AC015804.14        186568-186589
            689-1015            AC021593.15        154767-155093       c
            1016-3619           AC021593.15        135161-137764       c
            3620-3835           AC021593.15        121672-121887       c
            3836-4002           AC021593.15        118686-118852       c
            4003-4083           AC021593.15        115444-115524       c
            4084-4141           AC021593.15        113515-113572       c
            4142-4284           AC021593.15        111331-111473       c
            4285-4434           AC021593.15        109275-109424       c
            4435-4581           AC021593.15        107068-107214       c
            4582-4701           AC021593.15        103443-103562       c
            4702-4785           AC021593.15        100023-100106       c
            4786-5026           AC021593.15        99516-99756         c
            5027-5194           AC021593.15        95002-95169         c
            5195-5386           AC021593.15        93485-93676         c
            5387-5530           AC021593.15        92846-92989         c
            5531-5647           AC021593.15        88748-88864         c
            5648-5842           AC021593.15        88072-88266         c
            5843-5913           AC021593.15        84065-84135         c
            5914-6053           AC021593.15        83056-83195         c
            6054-10391          AC021593.15        77787-82124         c
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..10391
                     /gene="TNRC6C"
                     /note="trinucleotide repeat containing adaptor 6C"
                     /db_xref="GeneID:57690"
                     /db_xref="HGNC:HGNC:29318"
                     /db_xref="MIM:610741"
     exon            1..566
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            567..666
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     CDS             604..6414
                     /gene="TNRC6C"
                     /note="isoform 1 is encoded by transcript variant 1;
                     trinucleotide repeat-containing gene 6C protein;
                     trinucleotide repeat containing 6C"
                     /codon_start=1
                     /product="trinucleotide repeat-containing gene 6C protein
                     isoform 1"
                     /protein_id="NP_001136112.2"
                     /db_xref="GeneID:57690"
                     /db_xref="HGNC:HGNC:29318"
                     /db_xref="MIM:610741"
                     /translation="MEEKKKKKQEEKKKKEGAQKKAADQKTKVPEPTKTCSSQPQPAG
                     TSTSTSTSTISSSNNGKRASASGQQPAASRYLPREVPPRFRQQEQKQLLKRGQPLPTG
                     TLTSVSPTQGAGPAGVSPPPLPGAGTQHHPSKLQPDLSHSGLADHYENSHWGQQPTYR
                     SEANCSWDKVIIDRTDKEAWPSITGTETESASECTTDTDSASNCGSENSSMATGSAQG
                     NFTGHTKKTNGNNGTNGALVQSPSNQSALGAGGANSNGSAARVWGVATGSSSGLAHCS
                     VSGGDGKMDTMIGDGRSQNCWGASNSNAGINLNLNPNANPAAWPVLGHEGTVATGNPS
                     SICSPVSAIGQNMGNQNGNPTGTLGAWGNLLPQESTEPQTSTSQNVSFSAQPQNLNTD
                     GPNNTNPMNSSPNPINAMQTNGLPNWGMAVGMGAIIPPHLQGLPGANGSSVSQVSGGS
                     AEGISNSVWGLSPGNPATGNSNSGFSQGNGDTVNSALSAKQNGSSSAVQKEGSGGNAW
                     DSGPPAGPGILAWGRGSGNNGVGNIHSGAWGHPSRSTSNGVNGEWGKPPNQHSNSDIN
                     GKGSTGWESPSVTSQNPTVQPGGEHMNSWAKAASSGTTASEGSSDGSGNHNEGSTGRE
                     GTGEGRRRDKGIIDQGHIQLPRNDLDPRVLSNTGWGQTPVKQNTAWEFEESPRSERKN
                     DNGTEAWGCAATQASNSGGKNDGSIMNSTNTSSVSGWVNAPPAAVPANTGWGDSNNKA
                     PSGPGVWGDSISSTAVSTAAAAKSGHAWSGAANQEDKSPTWGEPPKPKSQHWGDGQRS
                     NPAWSAGGGDWADSSSVLGHLGDGKKNGSGWDADSNRSGSGWNDTTRSGNSGWGNSTN
                     TKANPGTNWGETLKPGPQQNWASKPQDNNVSNWGGAASVKQTGTGWIGGPVPVKQKDS
                     SEATGWEEPSPPSIRRKMEIDDGTSAWGDPSNYNNKTVNMWDRNNPVIQSSTTTNTTT
                     TTTTTTSNTTHRVETPPPHQAGTQLNRSPLLGPVSSGWGEMPNVHSKTENSWGEPSSP
                     STLVDNGTAAWGKPPSSGSGWGDHPAEPPVAFGRAGAPVAASALCKPASKSMQEGWGS
                     GGDEMNLSTSQWEDEEGDVWNNAASQESTSSCSSWGNAPKKGLQKGMKTSGKQDEAWI
                     MSRLIKQLTDMGFPREPAEEALKSNNMNLDQAMSALLEKKVDVDKRGLGVTDHNGMAA
                     KPLGCRPPISKESSVDRPTFLDKDGGLVEEPTPSPFLPSPSLKLPLSHSALPSQALGG
                     IASGLGMQNLNSSRQIPSGNLGMFGNSGAAQARTMQQPPQPPVQPLNSSQPSLRAQVP
                     QFLSPQVQAQLLQFAAKNIGLNPALLTSPINPQHMTMLNQLYQLQLAYQRLQIQQQML
                     QAQRNVSGSMRQQEQQVARTITNLQQQIQQHQRQLAQALLVKQPPPPPPPPHLSLHPS
                     AGKSAMDSFPSHPQTPGLPDLQTKEQQSSPNTFAPYPLAGLNPNMNVNSMDMTGGLSV
                     KDPSQSQSRLPQWTHPNSMDNLPSAASPLEQNPSKHGAIPGGLSIGPPGKSSIDDSYG
                     RYDLIQNSESPASPPVAVPHSWSRAKSDSDKISNGSSINWPPEFHPGVPWKGLQNIDP
                     ENDPDVTPGSVPTGPTINTTIQDVNRYLLKSGGSSPPSSQNATLPSSSAWPLSASGYS
                     SSFSSIASAPSVAGKLSDIKSTWSSGPTSHTQASLSHELWKVPRNSTAPTRPPPGLTN
                     PKPSSTWGASPLGWTSSYSSGSAWSTDTSGRTSSWLVLRNLTPQIDGSTLRTLCLQHG
                     PLITFHLNLTQGNAVVRYSSKEEAAKAQKSLHMCVLGNTTILAEFAGEEEVNRFLAQG
                     QALPPTSSWQSSSASSQPRLSAAGSSHGLVRSDAGHWNAPCLGGKGSSELLWGGVPQY
                     SSSLWGPPSADDSRVIGSPTPLTTLLPGDLLSGESL"
     exon            667..688
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            689..1015
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..3619
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3835
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            3836..4002
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4003..4083
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4084..4141
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4142..4284
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4434
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4435..4581
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4582..4701
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4702..4785
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            4786..5026
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5027..5194
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5195..5386
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5387..5530
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5531..5647
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5648..5842
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5843..5913
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            5914..6053
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     exon            6054..10391
                     /gene="TNRC6C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9000..9005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNRC6C"
                     /note="hexamer: AATAAA"
     polyA_site      9032
                     /gene="TNRC6C"
                     /note="major polyA site"
     regulatory      10369..10374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNRC6C"
                     /note="hexamer: ATTAAA"
     polyA_site      10391
                     /gene="TNRC6C"
ORIGIN      
        1 agttagagca acaagatggc cgcggtggca gctccgcacc tcagcgcgac ggccccggga
       61 gccgtagccg ccgccgccgg ccccgccgcc gcgcagtctt gagcgccgcc cgggccgccc
      121 cctgacaggg cagtcccagc gcccgccgcg ctcccgccgc cgtcaccgtc gcagtagccg
      181 cgggcccgcc cgagccggag gcggagggag cggagcggcc ccgcgcccag ccgtccgcag
      241 ctgccaccgc cgccgccgcc gccgcctagg acccggaccc cgccggagcc gcatcccgcc
      301 gggcgtgagg aggaggacgc gtcgcggcgt tgccggggcc ggacctggac ctgccgggac
      361 gcgccgccgc ccactcgccc gcgccgccgc cgcagccgcc gccgccgccc gcgccgggac
      421 ctgagtccgt ggcgccggcg ctgagcgggc ccccgccgcc cgcgcactcc cgaggccctc
      481 gggcggttgt tcgtgccgcc gccgccgcca ggcccgcgcc accggagccc cgcggagccg
      541 ccgccgccgc cggggacgga gcccagggag aaagagcaag aaacacaaga agaggaacgt
      601 ttgatggaag aaaagaaaaa gaaaaagcag gaagaaaaga aaaagaagga aggtgctcag
      661 aaaaaggctg ctgatcaaaa aaccaaagtg ccagaaccta ctaagacctg ttcaagccag
      721 ccccaacctg ccggtaccag taccagtact tccaccagca ctatctcaag cagcaacaat
      781 ggcaaacgtg catctgccag tggccagcag ccagctgcat cccgttacct gcctcgtgag
      841 gtgcctccac gcttccgcca gcaagaacag aagcagctat taaagagagg ccagccattg
      901 cctacgggga ctctaaccag tgtgagccca acccagggtg ctgggcctgc aggggtaagc
      961 ccacctcccc tacctggagc cggaacacag catcatccca gtaagctcca accagatctc
     1021 agtcatagtg gattggcaga tcattatgaa aattcccact ggggacagca gcctacttac
     1081 agaagcgaag ccaactgcag ctgggataaa gtgataatag ataggactga caaggaggcg
     1141 tggccttcca tcacaggaac agagactgaa tctgcctcag aatgtactac agacactgac
     1201 tctgcctcca actgtggctc agagaacagt agcatggcta cagggagtgc ccagggcaac
     1261 ttcactggac ataccaagaa gacaaatggc aataatggca ccaatggcgc actcgtccaa
     1321 agcccttcta atcagagtgc ccttggagca gggggagcga acagtaatgg aagtgcggcc
     1381 agagtgtggg gtgtagccac aggctccagc tctggcctgg ctcactgctc tgtcagtggt
     1441 ggggatggaa aaatggacac tatgattgga gatgggagaa gtcagaattg ctggggtgct
     1501 tccaactcca atgctggcat taatcttaac cttaatccta atgccaaccc agctgcctgg
     1561 cctgtacttg gacatgaagg aaccgtggcg acaggcaacc cttccagtat ttgcagtcca
     1621 gtcagtgcca taggtcaaaa tatgggcaac cagaacggga acccaacagg cactttaggt
     1681 gcttggggaa acttgctgcc acaagagagc acagaaccac aaacgtccac ttctcagaat
     1741 gtgtctttca gcgcacaacc tcagaacctt aacactgatg gaccaaataa cactaacccc
     1801 atgaactctt cacccaaccc tatcaatgca atgcagacaa atggactgcc aaactggggc
     1861 atggctgttg gtatgggggc catcatcccg ccccacctgc aaggccttcc tggtgctaat
     1921 ggatcatcag tttctcaagt cagtgggggc agtgctgaag gaataagcaa ttctgtgtgg
     1981 ggactgtccc caggtaaccc tgccacagga aatagcaatt ctgggttcag tcaggggaat
     2041 ggagacactg tgaactcagc attaagtgct aaacaaaatg gatccagcag tgctgtgcaa
     2101 aaggaaggaa gtggaggaaa tgcttgggat tcaggacctc ctgctggtcc tggaatactc
     2161 gcctggggaa ggggcagtgg caacaatggc gttggtaata tccattcagg agcttggggc
     2221 caccccagcc gaagcacctc taacggtgtg aatggggaat ggggaaagcc cccaaaccag
     2281 cattccaaca gtgacatcaa tgggaaagga tcaacagggt gggagagtcc tagtgtcacc
     2341 agccagaacc ctaccgtaca gcctggtggt gaacacatga actcctgggc caaagcggca
     2401 tcttctggaa ctacagcaag tgaaggaagt agtgatggtt ctggcaacca caatgaagga
     2461 agcactggga gggaaggaac gggagaaggc cgaaggcgag ataaagggat tatagaccaa
     2521 gggcacatcc agttgccaag gaatgatctt gacccaagag ttctgtctaa tactggttgg
     2581 ggacagactc ctgtaaagca aaacactgcc tgggaatttg aagaatcccc taggtctgaa
     2641 aggaaaaatg acaatgggac agaggcctgg ggttgtgcag ctactcaggc ttcaaactca
     2701 ggggggaaga acgatgggtc catcatgaac agtacaaata cctcttcagt atctgggtgg
     2761 gtcaacgcgc cacctgccgc tgtgccagca aacacaggtt ggggagacag caacaacaaa
     2821 gcgccaagtg gcccgggggt ttggggggac tcgataagct ctactgctgt tagtactgct
     2881 gctgctgcca agagtggcca tgcttggagt ggggccgcaa atcaggagga caagtcaccc
     2941 acctggggtg agcctccaaa gcccaaatcc caacactggg gagatggaca aagatcaaat
     3001 ccagcctgga gtgcaggagg gggagattgg gcagattcat cgtctgtcct tggacacttg
     3061 ggggatggga aaaaaaatgg atctggatgg gatgctgaca gtaataggtc agggtctggt
     3121 tggaatgaca ccacgagatc tgggaacagt ggctggggca acagcacaaa tacaaaggcc
     3181 aatccaggta caaactgggg ggagacttta aaacctggcc cccaacagaa ctgggctagc
     3241 aaaccccaag acaacaatgt gagtaactgg ggaggagctg cttctgtgaa acagacagga
     3301 acagggtgga tcggggggcc ggtaccggtc aaacagaagg acagcagtga agcaactggc
     3361 tgggaagaac cctctccacc gtccattcgc cgcaaaatgg aaattgatga tggtacctca
     3421 gcttgggggg acccaagcaa ctataacaat aaaactgtaa acatgtggga tagaaacaac
     3481 ccggtcatcc agagcagtac cacgaccaat accaccacca ccaccaccac taccacgagc
     3541 aacaccacac acagggtcga gacgccgccc ccgcaccagg ccggtactca gctgaatcga
     3601 tcaccgttgc ttggtccagt ttcatcaggc tggggagaaa tgcctaatgt tcactcaaag
     3661 actgaaaact cttggggaga accatcctcc ccttctaccc tggtggataa tggcacagca
     3721 gcatggggga agccacccag cagtggcagc gggtggggag atcaccctgc agagccgccg
     3781 gtggcatttg gaagagctgg cgcacctgtt gctgcctcag ccctgtgcaa accagcttca
     3841 aaatctatgc aagaaggctg gggcagtggt ggggatgaaa tgaacctcag taccagccag
     3901 tgggaggatg aagaagggga cgtgtggaat aatgctgctt cccaagaaag cacctcctcc
     3961 tgcagctcct gggggaacgc ccccaaaaaa ggacttcaaa agggcatgaa gacgtctggc
     4021 aagcaggatg aggcctggat catgagccgg ctgatcaaac aactcacaga catgggcttc
     4081 ccgagagagc cagctgagga ggccttgaag agtaacaata tgaatcttga tcaggccatg
     4141 agcgctctgc tggaaaagaa ggtggacgtg gacaagcgtg ggctgggagt gaccgaccat
     4201 aatggaatgg ccgccaagcc cctcggctgc cgcccgccaa tctccaaaga gtcttccgtg
     4261 gaccgcccca cctttcttga caaggatggc ggcctcgtgg aagagcccac gccttcaccg
     4321 ttcttgcctt ccccaagcct gaagctcccc ctttcacaca gtgcactccc cagtcaggcc
     4381 ctgggtggga ttgcctccgg gctgggcatg caaaacttga attcttctag acagataccg
     4441 agtggcaatc tgggtatgtt tggcaatagt ggagcagcac aagccaggac catgcagcag
     4501 ccgccacagc caccagtgca gcctcttaac tcttcccagc ccagtctccg tgctcaagtg
     4561 cctcagtttc tatcccctca ggttcaagca cagcttttgc agtttgcagc aaaaaacatt
     4621 ggtctcaacc ctgcactatt aacctcgcca attaatcctc aacatatgac gatgttgaac
     4681 cagctctatc agctgcagct ggcataccaa cgtttacaaa tccagcagca gatgttacag
     4741 gcccagcgta atgtgtccgg atccatgaga caacaggagc agcaagttgc gcgcacaatc
     4801 actaatctgc agcagcagat ccagcagcac cagcgccagc tggcccaggc cctgctcgtg
     4861 aagcagccac caccgccacc gcccccgccg cacctgtctc tgcacccctc tgcaggcaaa
     4921 tcggccatgg acagcttccc ctcgcaccca cagactcccg gcctacctga cctgcagacc
     4981 aaagagcagc agtcttcacc caacaccttt gctccttacc ctctcgctgg actgaaccca
     5041 aacatgaatg tcaacagcat ggacatgacc ggtggcttgt cggtgaagga cccatcccag
     5101 tcccagtcac gcctccccca gtggacgcac cccaactcca tggataactt gcccagtgcc
     5161 gcttcccccc tggagcagaa ccctagcaag catggtgcta tccctggagg tctaagcatt
     5221 gggcctccag gtaagtcctc cattgatgac tcctatggcc ggtacgattt aatccagaac
     5281 agtgagtcac cagccagtcc tcccgtagct gttccccata gctggtcacg tgccaaatct
     5341 gacagtgata aaatctcaaa tggctctagc atcaactggc ccccagaatt ccatccggga
     5401 gttccatgga aaggactgca gaatattgac cctgagaatg accctgacgt cactcctggc
     5461 agtgtcccca ctgggcctac catcaacacc accatccagg atgtcaaccg ctacctcctc
     5521 aagagtggag ggtcctcccc gccatcatct cagaatgcca cgctgccttc ttcgagtgcc
     5581 tggccactca gtgcctccgg ctacagtagc tctttcagca gcattgcatc cgcacctagt
     5641 gttgcaggta aactgtcaga catcaaatcg acgtggtcct ctggccctac ctcccacacg
     5701 caagcctctc tgtctcatga actatggaag gtgcccagaa acagtactgc acccacgagg
     5761 ccacctccag ggttaaccaa tcccaagccc tcctccacct ggggtgccag ccccctcggc
     5821 tggaccagct cctactcctc gggttctgcc tggagcaccg acacctcagg aagaaccagc
     5881 agctggctcg ttcttcgaaa cctcactccc cagattgatg gttctacact gcggacattg
     5941 tgtttgcaac atgggcctct tatcacattc cacctgaatc tgactcaagg caatgctgtg
     6001 gtccggtaca gctccaagga ggaggctgcc aaggcccaga agtctctgca catgtgcgtc
     6061 ctgggaaaca ctaccatcct ggccgagttc gctggtgaag aagaagtgaa tcgcttctta
     6121 gcccaaggcc aggcgctgcc acccacttcc agctggcagt ccagcagcgc gtccagccag
     6181 ccgcggctca gcgcagcggg cagctcccat ggcctggtac gcagcgacgc tggccactgg
     6241 aacgccccgt gcctgggtgg caaggggagc agtgagctgc tgtggggcgg ggtgccccag
     6301 tactccagca gcctgtgggg cccgcccagc gccgacgaca gcagggtgat aggcagcccc
     6361 acgccgctaa ccaccctgct gcctggggac ctgctcagcg gggagtccct gtaggctctg
     6421 ccatcatcag caccaggaga gccgacccct cccgggaccc ctcccggctg ggcggcccca
     6481 cagacccgct ggaacccagc agcggccgcc cttttgagta cctctgtcca ggactgaaga
     6541 cgaaccttgg ccgcagtcct tgcgaactgt tcgcaaaaca gtgcgggctg cggtgggtca
     6601 gcgtcacatg ctaatgacag gatgttcctc gtagcttttt attttgtgcg tcttatgttt
     6661 gtatagtgtg ttgtcatttt gatttttttt ttttttttaa gtataaaagc tgcttagaat
     6721 agttctatca tgaagggcac ttttcagatt gtgggctgga aagggttatt ttatctgtgg
     6781 gggagggagg gagacgtgaa gcctatttaa aatgagcctg tgacgattat gcacatgacc
     6841 agaggcggcc ggtaatcagg gcagagtctg gtgtggaccg ccgggcgcag agcggctccg
     6901 cggcgggagc tgagcggaag gcgcgggctg cgatctgctc tctgttcccc tggatccgcc
     6961 tatgcacatt acccttgttt cattactttt tcatgtcatt tttttatccc aaaaaatatt
     7021 ttttaaaagt taaaaaaaga catatcaaac atgcaaatac ttgaatccag caggccaata
     7081 gtgaaatgtg aacactttct aattctacac ttccaggttg acatcagtac acagaaacag
     7141 gctctaggaa aaatttctaa gttcatagcc tacgtgtgtg tatgtgtgcg tgttgaaaca
     7201 gaagtgtgtg tgtgtgtgca tgtgtggcgc gtgctcctgc acggcgtgca gttttctgtg
     7261 tttgtgtgtt tgaaagcgtg tgaggattta actgtgggtt ttcccttgta ttcagtatac
     7321 gctttttttt tggcgcattg gtcaaacggt tgttattatt agcttctaac tttgcactga
     7381 gtgtcctcgc ccttccttta gctaatccta tacattacag agaattccta ggacagggct
     7441 ggcgacaatt catgtgtaaa tgtttactca aggactctgt gactgcgttt aaaaagtaca
     7501 gtgtatatct ctggagaaat aacatgtata cctaccttta gcagcttttt attgtggact
     7561 aatgcaagaa aattattacc agagtattgc accatttttc catttggaat ataaagttaa
     7621 agagaaaaaa aaaaaaaact gttgttgaac tgtggccata gatacttgta aagaatggat
     7681 ggttccgtgg aaggaacaga aacaagcact tgggcgtggg ttgtgactgc ttttggggga
     7741 gcctggggtt tgggctccca cctgtttaca gacctttttt tctccttcag actttaaaat
     7801 tttaaaaatt taaaaaggaa ttaaaaaaaa aaaaagactt ccgtcgtaaa gaaacctatt
     7861 ttcagaatgc agatgcgttc cttcagagct ctgggacggg acagtgttgt tccctcggcc
     7921 atcgtcgtgg actctgtcga ttgctttcgc cccttcgtca aggtgacagg cgttgtggcc
     7981 actgtcttgc acatgccgga cgcgctctca ggaaagccag ggctcccgag ggcagctcca
     8041 caccatttca tgtattttta aacccaactc ctagcactga agatgttatt taaaaaaaaa
     8101 aaaaacaaaa caaaacaaaa aaatgaaaaa aaagaaaaag aggaaaaaaa aacacaaaga
     8161 agaaaaatac ctaatctcta atgtgtagca gttacatatt taccaaataa tggactcaaa
     8221 agaaatagat gtttgaagag tgctttatat attaaaaatt gctttatgtt ttaaaaatga
     8281 tcaatgtttt gattgttttg caaggaagaa agacaatgga ataacatacc ttcaagtatg
     8341 ttttaaaaaa tatatatatg aacattgtgc tccctgcctg cttgatcagg aaacttgtgc
     8401 attacatttt tttaattagc tttttaatgg ttttatcaaa acaaaaaaaa tacaaaaaaa
     8461 aaaaaaaaag tcctgcaaga ctgcagatcc acaaaagctg gttttataga ggatcatgaa
     8521 ctatgcacaa actgggttca agactttttt tctcaagttt agtaggatat ttagaaaaac
     8581 caaccttcct cagagtgact gcatctcatt gccattacat gcgttctata tattcgatgg
     8641 acatacacct gcgtatgtat atccaatatg tgtgcgtgtg agcatgcgtg tgcgtatgtg
     8701 cgtccgacat accacttccc gtgctgccgg cgagcattga gttgtagaaa aactgttgtt
     8761 ttaaaattgg ggatggaagt caagaatatg tttgtttgtt tttgggtttt gttgggtttg
     8821 aggggttttt tgtttgtttg ttttttgttt tttttacctt ttattttgaa cttaatatgt
     8881 ctgtaactct gagattaaac aacaagttcg tggctcttaa tgttttcctc atagaatgtg
     8941 actgttgaag aacatgcatc gaaagttgct ttcgaaagta caacgcttta ttgaatctta
     9001 ataaattgca atttttttct tggctgttca aagtaatgtg tttttcattt ttcaaaagac
     9061 actatggatg tctactttgc acgctgcgat tgggagagct gtcccgctgc atgcgttccc
     9121 tctgtaattt cctcagagct cacatacgta cctctctcac gagtgaactc agattttcca
     9181 ttgttttgct ttattctatc atttgctttg tggttttgct gtaaatattg aaatcttaat
     9241 catcatcgag aggcacagcc aagctttcca gctctccacc cccgtggccc atccaagtct
     9301 gttcatctgg taacttctgt tgtctgggac ggcagcaaga agatgccggg gcttgcctgg
     9361 agtcctggca gaggagtgcc actcactttt gcctagggtc cagttggggc ttaaaaaata
     9421 tttggagaga agagtaatga ggattttttg tgtttcctag acattttatg catctatttt
     9481 tgtaaaatca ccttcgctaa ctttcaccaa gcactttgag cacagtggaa cttcagaagc
     9541 acaaccagcc taaccccaga atctcaggtg gcacagctag aaattgagtc ccttcctagg
     9601 acactggccg tggccttcag ggtgaccagt cagtgccacc gggttggcat tggtgttaca
     9661 gacaggcctc tgaagacaaa ccgagcaccc cagcggcccc tcaaactaag caaggatctt
     9721 ttttctgtct gcgctccctg gggcaaggtc aaaggtggac ccgcccactg tgggtcaatt
     9781 ccttcgaaaa gatgggaccc gcacccccgc cacaggctgg ccccccccca cccatgggga
     9841 aggtggtgtg ctgctggctc tgaccatact cttttggaaa ttgagaagga aagcattgag
     9901 tgggacctaa tccggagaag aaattaaaga ccagaaaaag aaggaagctg ggaatgaaac
     9961 tcaaaatgca cttgaacctg gaagcggcaa ccctcagctc tgcgcggccg agcctcagca
    10021 agagttcgtt ttgtgcctga gttactacta caggggcaaa ctgtacccgt gcctgtgcgc
    10081 tgggagaaca aggtcagcgc ccaagggtgt gcaaaggatg tggcaggctt catggaggag
    10141 gtggcatttg ccctgggcct gggagaccag gttggatttt gacagactgg gaactcgggg
    10201 gacaggaggg cagcacagtg taagctaaag ccctgtgtag ggaccccacg caaagacctc
    10261 gtgggcctgg gtgtccaggg caccatttcc acccatgggt gaaaaagagg cttgaggaag
    10321 acaacaggtt cttcacctgc atctgtggat atatattttt ttaagtttat taaatttttt
    10381 tttaaatggc a
//
