LOCUS       NM_014494               8491 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A),
            transcript variant 1, mRNA.
ACCESSION   NM_014494 NM_020847
VERSION     NM_014494.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Li,M., Zhai,P., Mu,X., Song,J., Zhang,H. and Su,J.
  TITLE     Hypoxic BMSC-derived exosomal miR-652-3p promotes proliferation and
            metastasis of hepatocarcinoma cancer cells via targeting TNRC6A
  JOURNAL   Aging (Albany NY) 15 (22), 12780-12793 (2023)
   PUBMED   37976119
  REMARK    GeneRIF: Hypoxic BMSC-derived exosomal miR-652-3p promotes
            proliferation and metastasis of hepatocarcinoma cancer cells via
            targeting TNRC6A.
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Majerciak,V., Zhou,T., Kruhlak,M.J. and Zheng,Z.M.
  TITLE     RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote
            biogenesis of stress granules
  JOURNAL   Nucleic Acids Res 51 (17), 9337-9355 (2023)
   PUBMED   37427791
  REMARK    GeneRIF: RNA helicase DDX6 and scaffold protein GW182 in P-bodies
            promote biogenesis of stress granules.
REFERENCE   3  (bases 1 to 8491)
  AUTHORS   Yang,R., Han,J. and Zhao,S.
  TITLE     LINC01082 Inhibits Non-Small Cell Lung Cancer by Targeting the
            miR-543/TNRC6A Axis
  JOURNAL   Biochem Genet 61 (4), 1585-1605 (2023)
   PUBMED   36719626
  REMARK    GeneRIF: LINC01082 Inhibits Non-Small Cell Lung Cancer by Targeting
            the miR-543/TNRC6A Axis.
REFERENCE   4  (bases 1 to 8491)
  AUTHORS   Chen,W., Ru,J., Wu,T., Man,D., Wu,J., Wu,L., Sun,Y., Yu,H., Li,M.,
            Zhang,G., Zhu,X., Tong,R., Xiao,H., Li,Y. and Yang,B.
  TITLE     MiR-652-3p promotes malignancy and metastasis of cancer cells via
            inhibiting TNRC6A in hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 640, 1-11 (2023)
   PUBMED   36495604
  REMARK    GeneRIF: MiR-652-3p promotes malignancy and metastasis of cancer
            cells via inhibiting TNRC6A in hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 8491)
  AUTHORS   Wakiyama,M. and Takimoto,K.
  TITLE     N-terminal Ago-binding domain of GW182 contains a tryptophan-rich
            region that confer binding to the CCR4-NOT complex
  JOURNAL   Genes Cells 27 (9), 579-585 (2022)
   PUBMED   35822830
  REMARK    GeneRIF: N-terminal Ago-binding domain of GW182 contains a
            tryptophan-rich region that confer binding to the CCR4-NOT complex.
REFERENCE   6  (bases 1 to 8491)
  AUTHORS   Eystathioy,T., Jakymiw,A., Chan,E.K., Seraphin,B., Cougot,N. and
            Fritzler,M.J.
  TITLE     The GW182 protein colocalizes with mRNA degradation associated
            proteins hDcp1 and hLSm4 in cytoplasmic GW bodies
  JOURNAL   RNA 9 (10), 1171-1173 (2003)
   PUBMED   13130130
REFERENCE   7  (bases 1 to 8491)
  AUTHORS   Eystathioy,T., Chan,E.K., Mahler,M., Luft,L.M., Fritzler,M.L. and
            Fritzler,M.J.
  TITLE     A panel of monoclonal antibodies to cytoplasmic GW bodies and the
            mRNA binding protein GW182
  JOURNAL   Hybrid Hybridomics 22 (2), 79-86 (2003)
   PUBMED   12831532
REFERENCE   8  (bases 1 to 8491)
  AUTHORS   Eystathioy,T., Chan,E.K., Tenenbaum,S.A., Keene,J.D., Griffith,K.
            and Fritzler,M.J.
  TITLE     A phosphorylated cytoplasmic autoantigen, GW182, associates with a
            unique population of human mRNAs within novel cytoplasmic speckles
  JOURNAL   Mol Biol Cell 13 (4), 1338-1351 (2002)
   PUBMED   11950943
  REMARK    GeneRIF: GW ribonucleoprotein complex is involved in the
            posttranscriptional regulation of gene expression
REFERENCE   9  (bases 1 to 8491)
  AUTHORS   Margolis,R.L., Abraham,M.R., Gatchell,S.B., Li,S.H., Kidwai,A.S.,
            Breschel,T.S., Stine,O.C., Callahan,C., McInnis,M.G. and Ross,C.A.
  TITLE     cDNAs with long CAG trinucleotide repeats from human brain
  JOURNAL   Hum Genet 100 (1), 114-122 (1997)
   PUBMED   9225980
REFERENCE   10 (bases 1 to 8491)
  AUTHORS   Yamagata,K., Takeda,J., Menzel,S., Chen,X., Eng,S., Lim,L.R.,
            Concannon,P., Hanis,C.L., Spielman,R.S., Cox,N.J. and Bell,G.I.
  TITLE     Searching for NIDDM susceptibility genes: studies of genes with
            triplet repeats expressed in skeletal muscle
  JOURNAL   Diabetologia 39 (6), 725-730 (1996)
   PUBMED   8781769
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002565.1, BE158958.1,
            AJ492221.1, AC008731.8 and BU743266.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014494.3.
            
            Summary: This gene encodes a member of the trinucleotide repeat
            containing 6 protein family. The protein functions in
            post-transcriptional gene silencing through the RNA interference
            (RNAi) and microRNA pathways. The protein associates with messenger
            RNAs and Argonaute proteins in cytoplasmic bodies known as
            GW-bodies or P-bodies. Inhibiting expression of this gene
            delocalizes other GW-body proteins and impairs RNAi and
            microRNA-induced gene silencing. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000395799.8/ ENSP00000379144.3
            RefSeq Select criteria    :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                AC002565.1         19574-19650         c
            78-244              BE158958.1         113-279             c
            245-282             AJ492221.1         54-91
            283-296             AC008731.8         186896-186909       c
            297-6080            AJ492221.1         106-5889
            6081-7810           AC008731.8         112136-113865       c
            7811-8491           BU743266.1         25-705              c
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.1"
     gene            1..8491
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="trinucleotide repeat containing adaptor 6A"
                     /db_xref="GeneID:27327"
                     /db_xref="HGNC:HGNC:11969"
                     /db_xref="MIM:610739"
     exon            1..196
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..6080
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="isoform 1 is encoded by transcript variant 1; CAG
                     repeat protein 26; glycine-tryptophan protein of 182 kDa;
                     trinucleotide repeat-containing gene 6A protein; EDIE;
                     GW182 autoantigen; EMSY interactor protein; CTD-2540M10.1;
                     trinucleotide repeat containing 6A"
                     /codon_start=1
                     /product="trinucleotide repeat-containing gene 6A protein
                     isoform 1"
                     /protein_id="NP_055309.2"
                     /db_xref="CCDS:CCDS10624.2"
                     /db_xref="GeneID:27327"
                     /db_xref="HGNC:HGNC:11969"
                     /db_xref="MIM:610739"
                     /translation="MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAA
                     QKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQ
                     QPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAA
                     NLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSST
                     SDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNT
                     GGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESK
                     SESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGIN
                     IQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEM
                     TGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLS
                     NGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVN
                     TNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQ
                     HSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRL
                     EEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSP
                     RGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGS
                     NCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDN
                     WGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESS
                     KPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLG
                     GPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGN
                     SRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSG
                     PSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEED
                     VEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPF
                     VKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSR
                     PQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGM
                     QNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVS
                     LLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGN
                     RPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHT
                     TPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQ
                     NSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPP
                     EFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLP
                     STSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKN
                     ITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLV
                     LKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLG
                     NTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHW
                     NGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGG
                     GESM"
     exon            197..244
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            245..332
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            333..354
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            355..780
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            781..3366
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..3543
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3544..3719
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3720..3752
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3753..3833
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3885
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3886..4028
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4175
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4176..4313
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4314..4442
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4443..4520
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4521..4731
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4732..4863
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4864..5022
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5023..5163
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5164..5271
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5272..5493
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5494..5564
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5565..5704
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5705..8491
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7704..7709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="hexamer: AATAAA"
     polyA_site      7737
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="major polyA site"
     regulatory      8471..8476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="hexamer: AATAAA"
     polyA_site      8491
                     /gene="TNRC6A"
                     /gene_synonym="CAGH26; FAME6; GW1; GW182; TNRC6"
                     /note="major polyA site"
ORIGIN      
        1 acttccggtc tggggcctgc ggcggcggcg gtgtcggcgg cggcggcggc ggcggcggcg
       61 gcggcggcag cgggtcggtg tagaaaatgg cgctggtgca gcggctcggg cctctccccg
      121 cggcgctgcg gagggcttga ggctcgcgag cctccttcgc cgcgccccac ttgctcgtgc
      181 actttacaca catgagagaa ttggaagcta aagctaccaa agacgtagaa agaaatctta
      241 gcagggattt agtgcaagaa gaagaacagt tgatggaaga aaagaaaaag aaaaaagacg
      301 acaagaaaaa gaaggaagct gctcaaaaga aggccactga acaaaaaatc aaagtgccag
      361 aacagataaa gcccagtgta agccagcctc agcctgccaa ctctaataac ggcacttcca
      421 cagcaaccag cactaataat aatgccaagc gagctacagc caacaatcag cagccacagc
      481 agcagcagca acagcagcag ccgcagcagc agcagccaca gcagcagcca cagccgcagc
      541 cgcagcagca gcagccacag cagcagccac aggccttgcc tcggtatcct cgtgaagtac
      601 ctccacgatt tcgccaccag gaacacaaac agcttctaaa gaggggtcag cattttcctg
      661 ttatagcagc aaaccttgga tctgctgtta aggtgttaaa cagccagtca gaaagcagtg
      721 ctttaacaaa tcaacagcca caaaataacg gagaggtgca gaacagcaaa aaccagtcag
      781 atataaacca cagtacttca ggatcccatt atgaaaattc ccagcgggga cctgtgtctt
      841 ctacaagtga ttctagcaca aactgtaaga atgctgttgt aagtgacttg tcggaaaaag
      901 aagcatggcc ctcagcccct ggcagtgatc cggagttggc ttcagaatgt atggatgctg
      961 attctgcctc cagttctgaa tcagagagaa acatcactat catggcttca gggaacacag
     1021 gtggtgaaaa agatggcctt cggaatagca ctggacttgg ttcccaaaac aagtttgtag
     1081 ttggtagcag cagcaataat gtgggccatg gaagtagtac tgggccatgg ggtttttccc
     1141 atggagccat aataagcaca tgtcaggtct ctgtggatgc tcctgaaagc aaatctgaaa
     1201 gtagcaacaa tagaatgaat gcttggggca ctgtaagttc ttcatcaaat ggagggttaa
     1261 atccaagcac tttgaattca gctagcaacc atggtgcctg gccagtatta gagaacaatg
     1321 gacttgccct aaaagggcct gtagggagtg gtagttctgg cattaatatt cagtgcagta
     1381 ctataggcca gatgcctaac aatcagagta ttaactctaa agtgagtggt ggttctaccc
     1441 atggtacctg gggaagcctt caggaaactt gtgaatctga agtaagtggt acacagaagg
     1501 tttcattcag tggtcaacct caaaatatta ccactgaaat gactggacca aataacacta
     1561 ctaactttat gacctctagt ttaccaaact ccggttcagt gcagaataat gagctgccta
     1621 gtagtaacac aggggcctgg cgtgtgagca caatgaatca tcctcagatg caggctccat
     1681 caggtatgaa tggcacttcc ctttctcacc ttagcaatgg agagtcaaaa agtggaggct
     1741 cttatggtac tacatggggt gcctatggtt ctaattactc tggagacaaa tgttcaggcc
     1801 ctaatggcca agctaatggt gacactgtga atgcaactct aatgcagcct ggcgtaaatg
     1861 gtcctatggg cactaacttt caagttaaca caaacaaagg aggtggtgtg tgggaatctg
     1921 gtgcagcaaa ctcccagagt acatcatggg gaagtggaaa tggcgcaaat tctggaggaa
     1981 gtcgaagagg atggggaacc cctgcacaaa acactggcac taatttaccc agcgttgagt
     2041 ggaacaaact gcctagcaat cagcattcca atgatagtgc aaatggcaat ggtaagacgt
     2101 ttacaaatgg atggaaatct actgaggaag aggatcaggg ttctgccaca tctcagacaa
     2161 atgagcaaag cagtgtgtgg gccaaaacag gaggtacagt ggagagcgat ggtagtacag
     2221 aaagcactgg acgccttgag gaaaaaggaa ctggggaaag tcagagtaga gacagaagaa
     2281 aaattgatca gcacacatta ctccaaagca ttgtaaacag aactgactta gatccacgtg
     2341 tcctgtccaa ctctggttgg ggacagactc ctattaagca gaatactgcc tgggatacag
     2401 aaacatcacc tagaggggaa cgaaagactg acaatgggac agaggcctgg ggaagctctg
     2461 caacacagac ttttaactca ggggcatgta tagataagac tagccctaat ggtaatgata
     2521 cctcatctgt atcagggtgg ggcgatccca aacctgctct gaggtgggga gattccaaag
     2581 gctcaaactg ccaggggggg tgggaagatg attctgctgc tacaggaatg gtcaagagca
     2641 atcagtgggg gaattgcaaa gaggagaagg ctgcatggaa tgactcgcaa aagaataaac
     2701 agggatgggg tgatggacaa aaatcaagcc aagggtggtc tgtttctgcc agtgataact
     2761 ggggagaaac ttcaaggaat aaccattggg gtgaggccaa taagaaatcc agctcaggag
     2821 gtagtgacag tgacaggtcc gtttccggtt ggaacgaact tggtaaaact agttctttca
     2881 cttggggaaa caacataaat ccaaataatt catcaggatg ggatgaatct tctaaaccta
     2941 ctccttccca gggatgggga gaccctccaa agtctaatca gtctctaggt tggggagatt
     3001 cgtcaaagcc agtcagctct ccagactgga acaagcaaca agacattgtt ggatcttggg
     3061 gaatcccacc agctacaggc aaacctcctg gtacaggctg gctgggggga cctataccag
     3121 ccccagcaaa agaagaagaa cccacaggct gggaggaacc atccccagaa tctatacgtc
     3181 gcaaaatgga gattgatgat ggaacttcag cttggggaga tccaagcaaa tacaactaca
     3241 aaaatgtgaa catgtggaac aaaaacgtcc caaatggcaa cagccgttca gaccagcaag
     3301 cacaggtaca tcagctgcta acgcctgcaa gtgccatctc aaacaaagag gcaagcagtg
     3361 gctctggctg gggtgagccc tggggggagc cttctactcc agccacaact gtggataatg
     3421 gtacttcagc atggggtaag cccatagaca gtggtcccag ctggggggaa cccattgctg
     3481 cggcatccag cacatccacg tggggctcca gctctgttgg tccacaagca ttaagcaaat
     3541 ctgggccaaa atctatgcaa gatggctggt gtggtgatga tatgccattg cctggaaatc
     3601 gccccactgg ctgggaagag gaagaggatg tggagattgg aatgtggaat agtaattcat
     3661 ctcaagagct taactcatct ttaaattggc caccatatac aaagaaaatg tcatcgaagg
     3721 gtctgagtgg caaaaaaagg agaagggaaa ggggaatgat gaaaggtgga aacaaacaag
     3781 aagaagcgtg gataaatcca tttgttaaac agttttcaaa catcagtttt tcgagagact
     3841 caccagagga aaatgtacaa agcaataaga tggacctttc tggaggaatg ttacaagaca
     3901 aacgaatgga gatagataaa catagcctaa atattggtga ttacaatcga acggtcggga
     3961 aaggccctgg ttctcggcct cagatttcca aagagtcttc catggagcgc aatccttatt
     4021 ttgataagga tggcattgta gcagatgaat cccaaaacat gcagtttatg tccagtcaaa
     4081 gcatgaagct tcccccttca aatagtgcac tacctaacca ggcccttggc tccatagcag
     4141 ggctgggtat gcaaaacttg aattctgtta gacagaatgg caatcccagt atgtttggtg
     4201 ttggaaacac agcagcacaa ccccggggca tgcagcagcc tccagcacaa cctcttagtt
     4261 catctcagcc taatctccgt gctcaagtgc ctcctccatt actctcccct caggttccag
     4321 tttcattgct gaagtatgca ccaaacaacg gtggcctgaa tccactcttt ggccctcaac
     4381 aggtagccat gctgaaccag ctatcccagc taaaccagct ttctcagatc tcccagttac
     4441 agcgattgtt agcgcagcag caaagggcgc agagtcagag aagcgtgcct tctgggaacc
     4501 ggccgcagca agaccagcag ggtcgacctc ttagtgtgca gcagcaaatg atgcaacaat
     4561 ctcgtcaact tgatccaaac ctgttggtga agcagcagac tccaccatct cagcagcagc
     4621 cactccatca gccagccatg aagtctttcc ttgacaatgt catgccccac actacacctg
     4681 agctgcaaaa agggccatca ccaataaatg ctttcagcaa cttccctata ggcttgaact
     4741 caaacttgaa tgtaaatatg gatatgaaca gtattaaaga gccacagtca agactaagga
     4801 agtggacgac agtggacagc atttctgtga acacatcttt ggatcaaaac tccagcaaac
     4861 atggtgctat ttcaagtggt ttcaggctgg aagagtctcc atttgttccc tatgacttta
     4921 tgaacagcag tacttcacca gccagtcctc caggttcaat aggagatggc tggccacgtg
     4981 ccaaatcgcc taacggctct agcagtgtta attggccacc agaatttcgt cctggtgagc
     5041 catggaaagg ttatccaaac attgaccctg aaactgaccc ttacgtcact cctggcagtg
     5101 tcataaacaa tctttcaatt aatactgtgc gggaagttga ccacctcagg gacaggaaca
     5161 gtgggtcatc ctcatccttg aacaccacgc tgccttcaac tagtgcctgg tcatccattc
     5221 gtgcctccaa ctacaacgtt cccctcagca gtacagcaca aagcacttca gccagaaata
     5281 gtgattccaa attgacatgg tctcctggtt cagttacaaa cacctctctg gctcatgagc
     5341 tgtggaaggt ccctttgcca cctaaaaaca tcactgctcc gtcccgccca cctccgggac
     5401 tgactggtca gaagccaccc ttgtctacgt gggataattc tccccttcgt ataggtggag
     5461 gatggggaaa ttctgacgcc agatataccc caggttccag ctggggtgag agcagctcag
     5521 ggagaataac aaattggctt gttctaaaaa accttacacc tcagatcgat ggctcaactc
     5581 tgcgcactct gtgcatgcag cacggcccgc tgatcacatt ccacctgaac ctccctcacg
     5641 gaaatgctct ggtccgctac agttcaaaag aagaggtagt gaaggcacaa aagtctctgc
     5701 acatgtgtgt actggggaac actactattc ttgctgagtt tgccagtgaa gaggagatca
     5761 gtcgtttctt tgcacaaagc cagtctctga ccccttctcc cggctggcag tctctcgggt
     5821 ccagccagag ccggctgggc tccctcgact gttcccactc attctccagc cggaccgatc
     5881 tcaatcactg gaatggtgct gggctgtcgg gaactaactg tggagacctt cacggcactt
     5941 cactctgggg gaccccgcat tattccacaa gcctgtgggg tcccccaagc agcagcgacc
     6001 cccgaggaat tagcagccca tctcccatta acgcttttct ttctgttgac cacctgggtg
     6061 ggggtggaga gtccatgtaa cagtgtagat gcagactcac cgaccgggac ctcagacgcg
     6121 agggaaagga gcactaagtg gggctcgccg cctgcagcca ggggccgcct gtgggaacag
     6181 ctattctctg cacattttcc actttgtttt ccccaaaaca tatcagtttg aatacttgaa
     6241 tcatgcaggc caatattata atgtgaaaag gtatctactc tatttacact cccaaatagc
     6301 gccatacatg ctaaaccgta gagaatgagc tcgcttgtgt ctattcatca tgtttagcct
     6361 ttggattctt tttttttttt tccttctatt cctccccaac cccccccccc gccccttttt
     6421 ttctctcttg caaaaccatt ttttgggctg ataacgtatg agcttttccc tttgcactga
     6481 atgatgttct ctccgtctca tcggcagtat ggggggcagc tgtcccagtg tcaatgttta
     6541 ctcaagggtg ttcttaggag gcgtgcgctc tctactatgc cttgatgttg cctaccttat
     6601 tgtggtatcg tggagtttaa aagatcaagt taggatgctg acttaggatt attaatgaaa
     6661 gtgttgcacc agttttttca tgttgtaaaa ctaaagaatt tcgctctgca gtttgaaaaa
     6721 ctgtggccac agctgtgact tgcagcccac ctgccaccca ggacgggccc tgcactttga
     6781 ataggctttc cattttgttt tggaggttct cactttgaac cttcttgttt acagattttt
     6841 ttgtttgttt tttgagaaaa aaaaatgttt actcttccat catttaaaaa aaatgtaaaa
     6901 gacaaaaaaa aaatggagga tgatttaaaa gatgctttct atctctggga aaaaggagca
     6961 gcatttggcc atgttctttt gtttttctat tcctgtccca aatcaaagag catggttctc
     7021 aggaaaacca gttccccagt ttaaaaaaaa aaaaaaaaat tccttgtagt ttcttagagg
     7081 aaaaaaagaa aaaccccaac ttttagcact gatactacat attgctctgt taaagaattt
     7141 tctctgccaa aaaaaaagaa aaaacaaaaa aacgcttaaa gctggagttt gacattctgc
     7201 tttcagatgc tgtcttttta ttagtgagtg atgatggttt gctaataatc aataggtaat
     7261 aattttttgt aatcccatca agtggctcca tatgtttctg ctctctcgtg actgtgttaa
     7321 tgtttaactg ttgtacctta aagccgaaat cagtaactat gcatactgta accaaggtat
     7381 tgggcttaca gagttgtttg ttgtataaag aaaattttaa atgttgttgc aaactaacga
     7441 gttacaccat tttaaacttt ctttcctccc cccttttttt gcccacaaat ggtattataa
     7501 tgcttgctta gtcaaagaag agagactaaa caagggtaaa aattttaaca gtacagaatt
     7561 tgccatcata tcattgcctt gattctaact gtttgtgtcc taagatgcaa aagaagtcag
     7621 tggcttttaa ctgtttacaa atagaatgtg attgtaaaat gtacagtttg gttgtgtttg
     7681 aattatgaaa tttcttcaga tataataaac catgactttt tggctgctca acattaattg
     7741 tctccttttt gtgaatttat ttgtaggctc ttttttataa tgaaagtttc aaagttgcta
     7801 tgtatgaggg ttctcataga gcaaccgatt aaaaatctaa gcaaatattt gaacatttta
     7861 tctgaactca tcacaatttc accctgaaat aatgtgagaa caatgggaaa ctgtagcttg
     7921 ctccttccca ccctctctga gcatctttgg gatcttgttg ctcaaaactc ttctgtgact
     7981 tcatcttccc caccatttgt gcccatctca agcctcagca agaaaccatg tggaacatga
     8041 agcttaatga cttgacagtg tactagtgtt aaactctcat acctctgtta caaagcgaga
     8101 aacgccacac ccggactggc cttttcttcc cccttcacgg ccctcgcttc tccctgcagg
     8161 agctcggggg cgaaacctgt gtatggattt cagtgtatga cttcagatca tgctccaact
     8221 tgccaggtgt gagctaatgt tgtcggacac cttactataa gcaaatgtta ttcagtgcgt
     8281 tcaatgtata ttgacttcca tactggtttt tccaaaaacc aaaggtagct ttgaaaaacc
     8341 atgtctggaa atgtttggag cgttaagctg attgaccttc tgaccttggg gctttgagta
     8401 gtatataatt cataactgcg ttaattgtat tgttaaagtg tttgggagtt ttttgcgctt
     8461 gttatgtgga aataaagtgt ttgatttaaa a
//
