LOCUS       NM_002270               8489 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens transportin 1 (TNPO1), transcript variant 1, mRNA.
ACCESSION   NM_002270
VERSION     NM_002270.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Mahajan,D., Madugula,V. and Lu,L.
  TITLE     Rab8 and TNPO1 are ciliary transport adaptors for GTPase Arl13b by
            interacting with its RVEP motif containing ciliary targeting
            sequence
  JOURNAL   J Biol Chem 299 (5), 104604 (2023)
   PUBMED   36907439
  REMARK    GeneRIF: Rab8 and TNPO1 are ciliary transport adaptors for GTPase
            Arl13b by interacting with its RVEP motif containing ciliary
            targeting sequence.
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Porschke,M., Rodriguez-Gonzalez,I., Parfentev,I., Urlaub,H. and
            Kehlenbach,R.H.
  TITLE     Transportin 1 is a major nuclear import receptor of the nitric
            oxide synthase interacting protein
  JOURNAL   J Biol Chem 299 (3), 102932 (2023)
   PUBMED   36690276
  REMARK    GeneRIF: Transportin 1 is a major nuclear import receptor of the
            nitric oxide synthase interacting protein.
REFERENCE   3  (bases 1 to 8489)
  AUTHORS   Fare,C.M., Rhine,K., Lam,A., Myong,S. and Shorter,J.
  TITLE     A minimal construct of nuclear-import receptor Karyopherin-beta2
            defines the regions critical for chaperone and disaggregation
            activity
  JOURNAL   J Biol Chem 299 (2), 102806 (2023)
   PUBMED   36529289
  REMARK    GeneRIF: A minimal construct of nuclear-import receptor
            Karyopherin-beta2 defines the regions critical for chaperone and
            disaggregation activity.
REFERENCE   4  (bases 1 to 8489)
  AUTHORS   Yang,T.J., Li,T.N., Huang,R.S., Pan,M.Y., Lin,S.Y., Lin,S.,
            Wu,K.P., Wang,L.H. and Hsu,S.D.
  TITLE     Tumor suppressor BAP1 nuclear import is governed by transportin-1
  JOURNAL   J Cell Biol 221 (6) (2022)
   PUBMED   35446349
  REMARK    GeneRIF: Tumor suppressor BAP1 nuclear import is governed by
            transportin-1.
REFERENCE   5  (bases 1 to 8489)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 8489)
  AUTHORS   Pollard,V.W., Michael,W.M., Nakielny,S., Siomi,M.C., Wang,F. and
            Dreyfuss,G.
  TITLE     A novel receptor-mediated nuclear protein import pathway
  JOURNAL   Cell 86 (6), 985-994 (1996)
   PUBMED   8808633
REFERENCE   7  (bases 1 to 8489)
  AUTHORS   Michael,W.M., Choi,M. and Dreyfuss,G.
  TITLE     A nuclear export signal in hnRNP A1: a signal-mediated,
            temperature-dependent nuclear protein export pathway
  JOURNAL   Cell 83 (3), 415-422 (1995)
   PUBMED   8521471
REFERENCE   8  (bases 1 to 8489)
  AUTHORS   Moroianu,J., Hijikata,M., Blobel,G. and Radu,A.
  TITLE     Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers:
            alpha 1 or alpha 2 subunit binds nuclear localization signal and
            beta subunit interacts with peptide repeat-containing nucleoporins
  JOURNAL   Proc Natl Acad Sci U S A 92 (14), 6532-6536 (1995)
   PUBMED   7604027
REFERENCE   9  (bases 1 to 8489)
  AUTHORS   Bukrinsky,M.I., Haggerty,S., Dempsey,M.P., Sharova,N., Adzhubel,A.,
            Spitz,L., Lewis,P., Goldfarb,D., Emerman,M. and Stevenson,M.
  TITLE     A nuclear localization signal within HIV-1 matrix protein that
            governs infection of non-dividing cells
  JOURNAL   Nature 365 (6447), 666-669 (1993)
   PUBMED   8105392
REFERENCE   10 (bases 1 to 8489)
  AUTHORS   Bukrinsky,M.I., Sharova,N., Dempsey,M.P., Stanwick,T.L.,
            Bukrinskaya,A.G., Haggerty,S. and Stevenson,M.
  TITLE     Active nuclear import of human immunodeficiency virus type 1
            preintegration complexes
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6580-6584 (1992)
   PUBMED   1631159
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC035140.7, U70322.1, U72069.1
            and AC008972.8.
            
            On Sep 14, 2020 this sequence version replaced NM_002270.3.
            
            Summary: This gene encodes the beta subunit of the karyopherin
            receptor complex which interacts with nuclear localization signals
            to target nuclear proteins to the nucleus. The karyopherin receptor
            complex is a heterodimer of an alpha subunit which recognizes the
            nuclear localization signal and a beta subunit which docks the
            complex at nucleoporins. Alternate splicing of this gene results in
            several transcript variants encoding different proteins. [provided
            by RefSeq, Jun 2018].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.565956.1,
                                           SRR14038195.2035854.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337273.10/ ENSP00000336712.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                AC035140.7         13366-13457         c
            93-750              U70322.1           85-742
            751-2809            U72069.1           717-2775
            2810-8489           AC008972.8         82105-87784         c
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..8489
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /note="transportin 1"
                     /db_xref="GeneID:3842"
                     /db_xref="HGNC:HGNC:6401"
                     /db_xref="MIM:602901"
     exon            1..92
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..2774
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /note="isoform 1 is encoded by transcript variant 1;
                     importin 2; karyopherin (importin) beta 2; importin beta
                     2; M9 region interaction protein; karyopherin beta-2"
                     /codon_start=1
                     /product="transportin-1 isoform 1"
                     /protein_id="NP_002261.3"
                     /db_xref="CCDS:CCDS43329.1"
                     /db_xref="GeneID:3842"
                     /db_xref="HGNC:HGNC:6401"
                     /db_xref="MIM:602901"
                     /translation="MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQK
                     LEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQNFPNGVTDFIKSE
                     CLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGAL
                     QKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALM
                     LHIDSFIENLFALAGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQ
                     DENVALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDE
                     TIPDSEQDIRPRFHRSRTVAQQHDEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALD
                     VLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCMQGMIPYLPELIPH
                     LIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRILDSNKRVQEAA
                     CSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNK
                     PEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLV
                     QKTLAQAMLNNAQPDQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQ
                     CMQDKMPEVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAI
                     GEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLENTAITIGRLGYVCPQEVAPML
                     QQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDFIFFCDAVASWINPKDD
                     LRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV"
     exon            93..206
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            207..282
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            283..432
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            433..539
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            540..673
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            674..755
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            756..878
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            879..997
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1058
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1227
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1380
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1606
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1778
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1865
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1973
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2132
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2220
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2319
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2415
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2491
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2591
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2666
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2809
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..8489
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4404..4409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /note="hexamer: AATAAA"
     polyA_site      4431
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
     regulatory      8470..8475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /note="hexamer: AATAAA"
     polyA_site      8489
                     /gene="TNPO1"
                     /gene_synonym="IPO2; KPNB2; MIP; MIP1; TRN"
                     /note="major polyA site"
ORIGIN      
        1 gttccgcagc catttcaggc cccggacagg aggcagtgcc gcttcggccg aaggcccgag
       61 cgcccgaggc gtctgggatg gtgtgggacc ggcaaaccaa gatggagtat gagtggaaac
      121 ctgacgagca agggcttcag caaatcctgc agctgttgaa ggagtcccag tccccagaca
      181 ccaccatcca gagaaccgtg caacaaaaac tggaacaact taatcagtat ccagacttta
      241 acaactactt gatttttgtt cttacaaaat taaaatctga agatgaaccc acaagatcat
      301 tgagtggtct tatcttgaag aataatgtga aagcacactt tcagaacttc ccaaatggtg
      361 taacagactt tattaaaagt gaatgtttaa ataatattgg tgactcctct cctctgatta
      421 gagccactgt tggtattttg atcacaacta tagcctccaa gggagaattg cagaattggc
      481 ctgacctctt accaaaactc tgtagcctgt tggattctga agattataat acctgtgagg
      541 gagcatttgg tgcccttcag aagatttgtg aagattctgc tgagatttta gacagtgatg
      601 ttttagatcg tcctctcaac atcatgattc ccaaattttt acagttcttc aagcatagta
      661 gtccaaaaat aaggtctcac gctgttgcat gtgtcaatca gtttatcatc agtaggactc
      721 aagctctaat gttgcacatt gattctttta ttgagaatct ctttgcatta gctggtgatg
      781 aagaaccaga ggtacggaaa aatgtgtgcc gagcacttgt gatgttgctc gaagttcgaa
      841 tggatcgcct gcttcctcac atgcataata tagttgagta catgctacag aggactcaag
      901 atcaagatga aaatgtggct ttagaagcct gtgaattttg gctaacttta gctgaacagc
      961 caatatgcaa agatgtactc gtaaggcatc ttcctaagtt gattcctgtg ttagtgaatg
     1021 gcatgaagta ctcagacata gatattatcc tacttaaggg tgatgttgaa gaagacgaaa
     1081 cgattcctga tagtgaacag gatatacggc cacgttttca ccgatcgagg acggtggctc
     1141 agcagcatga tgaagatgga attgaagagg aagatgatga tgatgatgaa attgatgatg
     1201 atgatacaat ttctgactgg aatctaagaa aatgttctgc tgctgccctg gatgttcttg
     1261 caaatgtgta tcgtgatgaa ctgctgccac atattttgcc ccttttgaaa gaattacttt
     1321 ttcatcatga atgggttgtt aaagaatcag gcattttggt tttaggagca attgctgaag
     1381 gttgcatgca gggcatgatt ccatacttgc ctgagcttat tcctcacctt attcagtgcc
     1441 tctctgataa aaaggctctt gtgcgttcca taacatgctg gactcttagc cgctatgcac
     1501 actgggtggt cagccagccg ccagacacgt acctgaagcc attaatgaca gaattgctaa
     1561 agcgcatcct ggacagcaac aagagagtac aagaagctgc ctgcagtgcc tttgctaccc
     1621 tagaagagga ggcttgtaca gaacttgttc cttaccttgc ttatatactt gataccctgg
     1681 tctttgcatt tagtaaatac cagcataaga acctgctcat tctttacgat gccataggaa
     1741 cattagcaga ttcagtagga catcatttaa acaaaccaga atatattcag atgctaatgc
     1801 ctccactgat ccagaaatgg aacatgttaa aggatgaaga taaagatctc ttccctttac
     1861 ttgagtgcct atcttcagtt gccacagcac tgcagtctgg attccttccg tactgtgaac
     1921 ctgtgtatca gcgttgtgta aacctagtac agaagactct tgcacaagcc atgctaaaca
     1981 atgctcaacc agatcaatat gaagctccag ataaagattt tatgatagtg gctcttgatt
     2041 tactgagtgg cctggctgaa ggacttggag gcaacattga acagctggta gcccgaagta
     2101 acatcctgac actaatgtat cagtgcatgc aggataaaat gccagaagtt cgacagagtt
     2161 cttttgccct gttaggtgac ctcacaaaag cttgctttca gcatgttaag ccttgtatag
     2221 ctgatttcat gccaatattg ggaaccaacc taaatccaga attcatttca gtctgcaaca
     2281 atgccacatg ggcaattgga gaaatctcca ttcaaatggg tatagagatg cagccttata
     2341 ttcctatggt gttgcaccag cttgtagaaa tcattaacag acccaacaca ccaaagacgt
     2401 tgttagagaa tacagcaata acaattggtc gtcttggtta cgtttgtcct caagaggtgg
     2461 cccccatgct acagcagttt ataagaccct ggtgcacctc tctgagaaac ataagagaca
     2521 atgaggaaaa ggattcagca ttccgtggaa tttgtaccat gatcagtgtg aatcccagtg
     2581 gcgtaatcca agattttata tttttttgtg atgccgttgc atcatggatt aacccaaaag
     2641 atgatctcag agacatgttc tgtaagatcc ttcatggatt taaaaatcaa gttggcgatg
     2701 aaaattggag gcgtttctct gaccagtttc ctcttccctt aaaagagcgt cttgcagctt
     2761 tttatggtgt ttaatctaat acacttaagc tgcagtccca aaattagggg tccttcagtc
     2821 ttggagacta taagggagcc tctgcaccca gggaaaatgt taccctttac aggggggaag
     2881 ggtaaaccag tagggaatac agtacaatcc caaccctact gggaggggcg ggagggaggt
     2941 gttgccgtca ctgtattaag tcgatgttgg gaaacgtttt aacatctgga gcctttgtgg
     3001 gtggaaatat gtctccagtt acaactccgc agtggatgtg aagaagcaaa aaaaaaaaaa
     3061 tctattcagt ctactcacaa aacagtacat tgtggaatat tatggggaat tgtaccaaaa
     3121 caagaaccat ataaatgatg cctagggaca agaaagagga acaattctat agcgcacaat
     3181 aaaggaaacc taagaatggg agttacaaat agtaaagaag cttttttttt ttttttaatt
     3241 taaagttttt ttatgtaagt tttcccacat gatggggcct tgttttgcat gttgatgaag
     3301 aactacacaa agaaaactaa tatagttaaa agtcagcttg ccttcccgta gtagaagcag
     3361 gttcttggaa gttacaattt aaggtacccc aaaaaagttg gaaataaaac aaaacaaaca
     3421 taaacaatga agcaccctgt gaaatgccaa atgagtcact ccttttacct tttttggggt
     3481 gggcagggag ggaggaataa atggggttgg gcatatcaaa ctaaagatga catcttaatt
     3541 ttgcattgaa cattaatgta gcggatataa tttgatgatt atacttcatt agatttaatt
     3601 tctaggccaa gatgttactt tttaaagtgc agtttaaggt tcaggcatgc attctggctc
     3661 atagtggttg aaagtaattt aaattagtgg gaaagtagca tgcttgcatc acatagagtg
     3721 agattggtat tcatttacct atgttgcgcc agtttgtgtt gcagtttacc aattcaatat
     3781 agccctgcat ttaaagttcc tttttaagat ttgtggattt tatttttatt aagaacatag
     3841 atatataaag tactgtagtt tacaggtagg ccttgaaata tcttttttag gatctgttag
     3901 gaataagatt gatattgtat tgtgtgtaac ctgcacaatg tggaaagctg atatacctgt
     3961 gcaaaatctt tgcctctgtg ctgtcagtgt gatgtgcttt ctgcatggtt atatactact
     4021 agtgatttta tcaaaacttc taaaatttaa attacgtggt aaaagatctg taaaaggctg
     4081 cataaatgtt agttggcaca taaagacaat tgtagaagtt gaaaaatgat tgctatattt
     4141 caatgtttat tcccactcaa catactgcct tctaagcttc cctttttttg ttcaaagcat
     4201 gatcttaaag atatgtttaa gttaatggat gtaatgcagg gttcctacac tgtattttgg
     4261 cgcatgttgg tggccctctg tgccctagat atatgcacac agggtgcaag ttaaaagcta
     4321 cagagtgaaa gttggtttgg atcctcttca tttcatttgt ttagcttttc tgttattttt
     4381 ctctacttac atgtattcct gtgaataaat ccttgttaag ttaacccttt acttttcctt
     4441 ccatgtgtat tttcttatat actgtgaatg tgaaaaccta actggtacac ttgatcttgt
     4501 gttcatatga aagtgcaagt ctttattaat ttggattgcc tgaacagtgt atcccatgat
     4561 gatgaaggaa aatggagaga tttttctttt taactctgct ggtcagagat gaagccacgc
     4621 ctttccattt ttcaatgctg catatttaat ctgcaacaaa aatgttaagc cataacagcc
     4681 tttttatatt ttagtttgtc acctttgcat tgcagaataa atactgaata accattttta
     4741 taagcagttg ctcctctttg catggttcta ttctctaaaa gtgttgaatg atacagccat
     4801 tgcactgaag tttgagctgt gtgcgtgtga atttatactg tttcaaaaat ttcaaatgca
     4861 tggtagcatg tagaaaggtt tttgttacta tgtgtgtgtg tgtgttttgt tttgttttgt
     4921 tctgtttttt aacgttcttt cacctttcct ctactttggc tttgcaaatt tcagtctgta
     4981 gagcctgaaa ttaaattatt ttatagtgcc tgatattttt aatattataa gttggattca
     5041 aagggtatac agcattttgt tttctagtac cattgcttca ctgggagctc aaaatttgcc
     5101 tccctgttag tctttaataa ctgagtagag tagctttttt attcttagat tatagtgata
     5161 ttctgtagtg cttcaaatac ttagaattct aatatgagag atagtttaat attctggaat
     5221 agagagtatt ttaaattgaa atgaccaaat tcgatttaaa acaaacacat aacaatcttc
     5281 agtaaagtta tttgaacctg tggttgacaa ttctgtattg actaaaggca atattgtcac
     5341 ctgtctcatt aaaaattagt ttccagttag gcagatgaat aacactatta aaaatgcacc
     5401 cagttttgta gtcacttgac tttttttttt tatttaaatg aaatttaatt tgaaaataga
     5461 aaataaaatt aggtaaatat gactggcaac ctgaatttga gttgggattc tttgtgtatt
     5521 tttccccctt gaggttattg tttcttccta atttatattt cagatacata gtgtagaaca
     5581 ggaatttgca gaagccattt aagttatctt ttgaggtaag ctctgattta gcatttattc
     5641 tgataaaatc taatacatca tgggatatat ataaagcaac ttaattcttg tggtgtagtc
     5701 ttaatagttt tgaatgttga ctgaatgtct ataaaattgt gagtttgtct ttgttacatt
     5761 ccagtgtttc tgcctcttgg catgcttaaa gcacggctta cttcatctgc tccttacaca
     5821 ctaaaatgct gttagtgtgc tcaactacag aaatagccgc tgctaagtga tgtagatttt
     5881 ctacttgaat atttttatgt tgtaggaacc tcaggaggtc agtgtttact gttttatata
     5941 tgccttcttt ttcctgtttg agcttctctc tttgaaggat tctaacagaa caaagctgct
     6001 gatcaaccta agttggaaac agaaagtgta attaatataa cttaaatgca tgtttggcag
     6061 ttccatgtta cagtccatta gcgagtgaac ttagtgccac aggtattcat ttattcagga
     6121 aagaattttg ctttttgtat tgtctaatct cttcaatgac ttcatactgt gtaaaaaaaa
     6181 atcacttttt aatatttttt ggttggttat ttcccaaata atcaacatgt aaataatctt
     6241 ttaagagcca gttctgatgc tttacattat tgctacttga ttttgttatg caagttagat
     6301 ttcagaagaa tagattttaa aacacttaac caagccatta catcttaaaa acaaaacaag
     6361 tagcatacat tttgtaatta acattgataa acactgtgat ttttttggtt aagacttttt
     6421 cattgatctg aattgcttaa attgcatata ttgtaaaata ggatcagatg tatattttaa
     6481 actaatttca atggattttc ctcttgaaat gctttgtcta ctcagttata aaatattcag
     6541 atacaagttt tatctcaggt gaatactctt gtattgtttt tgctttggtg acatgcttat
     6601 aaagggtcat cctagttaca ttctttgttt tttaagtgta tttcattgag aatctttcca
     6661 tttagtctga tttttttcct aatattctag caggctgggg tggtataaag ccctcctgac
     6721 aagccttttg cctacaggtt tacctgaaaa tgagctacat atggtcgtca ttttcttcaa
     6781 actctcaaaa taggaaagtg gaaatttttc tcccctatat aaaattattc tgcctagcag
     6841 aaatgccaga aataaattaa ttgcatctta gtctaaattt aagtcctttg ccatgaggaa
     6901 aaagtggttt tttgcttcat atggtaaatc tatattattc atattgaatg tattaacaga
     6961 taatggtgca aaagcattct tcccagggga agagtgtatc atgcataact gcaatttaag
     7021 tccttccttt gataatactt caaaacatac acagctttgc ttactgaatt atttttgcaa
     7081 ataatacaag gaggggaaac taacttgcta ctagtgttgt taatgaagta taggccttgg
     7141 aacatttaag aaaactggtc ttcagatagt taagggcttg ggataattta agtgaaaagt
     7201 gagatcagta atgagctgtg ttgttaacaa tattatctgg taataatacc atgcaatata
     7261 gcgttgtata ccaacttagt gcattttata ctgtattaaa tatggagata cttctaatgc
     7321 ctctctaaaa ttaaatttta cataaaagtt agttttattc acctttaggc tttccaagtg
     7381 aattgtgact aattttttca atcttacata gatctttcac ccattagcat tacttacgta
     7441 gataattctt tatgcctagt tattatacat attaattttt aaggtataca tttaaattac
     7501 acaattgttc attgtggttt gtatcccaga atgtgttgtg ttttttaaaa gatgcataat
     7561 agctgaatgt atgcatgact ttgaaagaag ttaaaatggt gatttttttt cacctcttgt
     7621 acattttaaa accaggccaa atctatttgc caagcagtgt atcactaata agaaaagcag
     7681 tttttccttt tattgcagtt tttgtttatc tgccatagaa tttccttata ctgtggcttg
     7741 gtattattca agattagcta tttcgctggt attacatctt tttaaaagcc tattataaca
     7801 tggttagcct ataaggcagt gttggtcccc ttctaatatt ggcctcataa aggggttcca
     7861 ctgtactttc cgcatattac tgtgttgttg ttttcctttg tggatatata agcaaattga
     7921 gcttgggtga tttttatgga gacaataatt agacaatact gtataattag ttttacttaa
     7981 tagattatca tcttgtgaga agagatgttt aaacgtggta aatcacttca tattacaaaa
     8041 cagttttaca cttaatatgt taacattggg tgcaataatt tagtagcatt agctttagtt
     8101 acaaatataa ctggatcttt ctgctgacaa cttaggttgt atgagttatg cttaaaagct
     8161 ttaaatctga tgtttcctgt acctgccaca ctatgttaga atgtgtcctt caaacatatc
     8221 ctcctgcaac ttctcaaact gtactaaatt gatatttctt gaagtctaac tctgtgctaa
     8281 cagatctcca ttttaaatag aatacggttt taatttttga taagctgctg aattttaaag
     8341 agagtttttt ggggccacca aatattttgg atcatgcaga gaatatatat tgtactgtag
     8401 taattttgta tttacatttg tatgatgtga cataatagat gtgaatgtta atcactgctt
     8461 gactatgtta ataaagttgt ttaactata
//
