LOCUS       NM_001387719            3910 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens tyrosine kinase non receptor 2 (TNK2), transcript
            variant 22, mRNA.
ACCESSION   NM_001387719
VERSION     NM_001387719.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Zheng,D., Wei,Z., Zhang,C., Liu,W., Gong,C., Wu,F. and Guo,W.
  TITLE     ZNF692 promotes osteosarcoma cell proliferation, migration, and
            invasion through TNK2-mediated activation of the MEK/ERK pathway
  JOURNAL   Biol Direct 19 (1), 28 (2024)
   PUBMED   38650011
  REMARK    GeneRIF: ZNF692 promotes osteosarcoma cell proliferation,
            migration, and invasion through TNK2-mediated activation of the
            MEK/ERK pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Hodder,S., Fox,M., Binti Ahmad Mokhtar,A.M., Mott,H.R. and Owen,D.
  TITLE     ACKnowledging the role of the Activated-Cdc42 associated kinase
            (ACK) in regulating protein stability in cancer
  JOURNAL   Small GTPases 14 (1), 14-25 (2023)
   PUBMED   37194323
  REMARK    GeneRIF: ACKnowledging the role of the Activated-Cdc42 associated
            kinase (ACK) in regulating protein stability in cancer.
            Review article
REFERENCE   3  (bases 1 to 3910)
  AUTHORS   Zhou,A., Zhang,W. and Wang,B.
  TITLE     Host factor TNK2 is required for influenza virus infection
  JOURNAL   Genes Genomics 45 (6), 771-781 (2023)
   PUBMED   37133719
  REMARK    GeneRIF: Host factor TNK2 is required for influenza virus
            infection.
REFERENCE   4  (bases 1 to 3910)
  AUTHORS   Kan,Y., Paung,Y., Kim,Y., Seeliger,M.A. and Miller,W.T.
  TITLE     Biochemical Studies of Systemic Lupus Erythematosus-Associated
            Mutations in Nonreceptor Tyrosine Kinases Ack1 and Brk
  JOURNAL   Biochemistry 62 (6), 1124-1137 (2023)
   PUBMED   36854171
  REMARK    GeneRIF: Biochemical Studies of Systemic Lupus
            Erythematosus-Associated Mutations in Nonreceptor Tyrosine Kinases
            Ack1 and Brk.
REFERENCE   5  (bases 1 to 3910)
  AUTHORS   Kan,Y., Paung,Y., Seeliger,M.A. and Miller,W.T.
  TITLE     Domain Architecture of the Nonreceptor Tyrosine Kinase Ack1
  JOURNAL   Cells 12 (6), 900 (2023)
   PUBMED   36980241
  REMARK    GeneRIF: Domain Architecture of the Nonreceptor Tyrosine Kinase
            Ack1.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3910)
  AUTHORS   Kato,J., Kaziro,Y. and Satoh,T.
  TITLE     Activation of the guanine nucleotide exchange factor Dbl following
            ACK1-dependent tyrosine phosphorylation
  JOURNAL   Biochem Biophys Res Commun 268 (1), 141-147 (2000)
   PUBMED   10652228
REFERENCE   7  (bases 1 to 3910)
  AUTHORS   Eisenmann,K.M., McCarthy,J.B., Simpson,M.A., Keely,P.J., Guan,J.L.,
            Tachibana,K., Lim,L., Manser,E., Furcht,L.T. and Iida,J.
  TITLE     Melanoma chondroitin sulphate proteoglycan regulates cell spreading
            through Cdc42, Ack-1 and p130cas
  JOURNAL   Nat Cell Biol 1 (8), 507-513 (1999)
   PUBMED   10587647
REFERENCE   8  (bases 1 to 3910)
  AUTHORS   Mott,H.R., Owen,D., Nietlispach,D., Lowe,P.N., Manser,E., Lim,L.
            and Laue,E.D.
  TITLE     Structure of the small G protein Cdc42 bound to the GTPase-binding
            domain of ACK
  JOURNAL   Nature 399 (6734), 384-388 (1999)
   PUBMED   10360579
REFERENCE   9  (bases 1 to 3910)
  AUTHORS   Satoh,T., Kato,J., Nishida,K. and Kaziro,Y.
  TITLE     Tyrosine phosphorylation of ACK in response to temperature
            shift-down, hyperosmotic shock, and epidermal growth factor
            stimulation
  JOURNAL   FEBS Lett 386 (2-3), 230-234 (1996)
   PUBMED   8647288
REFERENCE   10 (bases 1 to 3910)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Tan,L. and Lim,L.
  TITLE     A non-receptor tyrosine kinase that inhibits the GTPase activity of
            p21cdc42
  JOURNAL   Nature 363 (6427), 364-367 (1993)
   PUBMED   8497321
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124944.8.
            
            Summary: This gene encodes a tyrosine kinase that binds Cdc42Hs in
            its GTP-bound form and inhibits both the intrinsic and
            GTPase-activating protein (GAP)-stimulated GTPase activity of
            Cdc42Hs. This binding is mediated by a unique sequence of 47 amino
            acids C-terminal to an SH3 domain. The protein may be involved in a
            regulatory mechanism that sustains the GTP-bound active form of
            Cdc42Hs and which is directly linked to a tyrosine phosphorylation
            signal transduction pathway. Several alternatively spliced
            transcript variants have been identified from this gene, but the
            full-length nature of only two transcript variants has been
            determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.879463.1,
                                           SRR18074969.3514972.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC124944.8         51461-51508
            49-229              AC124944.8         58797-58977
            230-300             AC124944.8         60356-60426
            301-522             AC124944.8         62370-62591
            523-675             AC124944.8         64094-64246
            676-953             AC124944.8         65075-65352
            954-1080            AC124944.8         68228-68354
            1081-1227           AC124944.8         68811-68957
            1228-1322           AC124944.8         69056-69150
            1323-1517           AC124944.8         74933-75127
            1518-1609           AC124944.8         77198-77289
            1610-2958           AC124944.8         78694-80042
            2959-3086           AC124944.8         80387-80514
            3087-3910           AC124944.8         83216-84039
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..3910
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="tyrosine kinase non receptor 2"
                     /db_xref="GeneID:10188"
                     /db_xref="HGNC:HGNC:19297"
                     /db_xref="MIM:606994"
     exon            1..48
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            49..229
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..3093
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="isoform 13 is encoded by transcript variant 22;
                     activated p21cdc42Hs kinase; activated Cdc42-associated
                     kinase 1; tyrosine kinase non-receptor protein 2"
                     /codon_start=1
                     /product="activated CDC42 kinase 1 isoform 13"
                     /protein_id="NP_001374648.1"
                     /db_xref="CCDS:CCDS93440.1"
                     /db_xref="GeneID:10188"
                     /db_xref="HGNC:HGNC:19297"
                     /db_xref="MIM:606994"
                     /translation="MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNE
                     DLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTS
                     PAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCL
                     KPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLR
                     KHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRA
                     LPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGL
                     NGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTD
                     MRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLS
                     AQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPPQ
                     HLGGVKKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPSARVPGTKASRGSGA
                     EVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPTRALPRPLHPTPVVDW
                     DARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVPEQARPPPP
                     LEDNLFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQAPAGSPAPSPSPGGDDKPQVP
                     PRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPASPPRVPPREPLSPQGSRTPSPLVPP
                     GSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAGPCILPIVRDGKKVSS
                     THYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPSTPAPAAPTATVRPMPQ
                     AALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMVEQLFGLG
                     LRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR"
     exon            230..300
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            301..522
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            523..675
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            676..953
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1080
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1227
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1322
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1517
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1609
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..2958
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3086
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            3087..3910
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3884..3889
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="hexamer: AATAAA"
     polyA_site      3910
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="major polyA site"
ORIGIN      
        1 gggaggcggc gcggagccgg ctgagcgcag ggccgagctg cggggcagag gctgggaggc
       61 ggcagaatgc agccagagga gggcacaggc tggctgctgg agctgctgtc cgaggtgcag
      121 ctgcaacagt acttcctgcg gctccgagat gacctcaacg tcacccgcct gtcccacttt
      181 gagtacgtca agaatgagga cctggagaag atcggcatgg gtcggcctgg ccagcggcgg
      241 ctgtgggagg ctgtgaagag gaggaaggcc ttgtgcaaac gcaagtcgtg gatgagtaag
      301 gtgttcagtg gaaagcgact ggaggctgag ttcccacctc atcactctca gagcaccttc
      361 cggaagacct cgcccgcccc tgggggccca gcaggggagg ggcccctgca gagcctcacc
      421 tgcctcattg gggagaagga cctgcgcctc ctggagaagc tgggtgatgg ttcctttggc
      481 gtggtgcgca ggggcgagtg ggacgcgccc tcagggaaga cggtgagtgt ggctgtgaag
      541 tgcctgaagc ccgatgtcct gagccagcca gaagccatgg acgacttcat ccgggaggtc
      601 aatgccatgc actcgctcga ccaccgaaac ctcatccgcc tctacggggt ggtgctcacg
      661 ccgcccatga agatggtgac agagctggca cctctgggat cgttgttgga ccggctacgt
      721 aagcaccagg gccacttcct cctggggact ctgagccgct acgctgtgca ggtggctgag
      781 ggcatgggct acctggagtc caagcgcttt attcaccgtg acctggctgc ccgcaatctg
      841 ctgttggcta cccgcgacct ggtcaagatc ggggactttg ggctgatgcg agcactacct
      901 cagaatgacg accattacgt catgcaggaa catcgcaagg tgcccttcgc ctggtgtgcc
      961 cccgagagcc tgaagacacg caccttctcc catgccagcg acacctggat gttcggggtg
     1021 acactgtggg aaatgttcac ctacggccag gagccctgga tcggcctcaa cggcagtcag
     1081 atcctgcata agatcgacaa ggagggggag cggctgcccc ggcccgagga ctgtccccag
     1141 gacatctaca acgtcatggt ccagtgctgg gctcacaagc cagaggacag acccacgttt
     1201 gtggccctgc gggacttcct gctggaggcc cagcccacag acatgcgggc ccttcaggac
     1261 tttgaggaac cggacaagct gcacatccag atgaatgatg tcatcaccgt catcgaggga
     1321 agggccgaga actactggtg gcgtggccag aacacacgga cgctgtgtgt ggggcccttc
     1381 cctcgcaacg tggtgacctc cgtggccggc ctgtcggccc aggacatcag ccagcccctg
     1441 cagaacagct tcatccacac agggcatggc gacagtgacc cccgccactg ctggggcttc
     1501 ccggacagga ttgacgaact gtatctggga aaccccatgg acccccccga cctcctgagc
     1561 gtggaactga gcacctcccg gcccccccag catctaggag gggtgaaaaa accaacctat
     1621 gaccctgtga gcgaggacca agaccccttg tccagcgact tcaagaggct gggcctgcgg
     1681 aagccaggcc tgccccgagg gctgtggctg gcgaagccct cggcgcgggt gccgggcacc
     1741 aaggccagcc gaggcagcgg ggctgaggtc acgctcatcg acttcggtga ggagcccgtg
     1801 gtcccggccc tacggccctg cgcgccctcc ctggcgcagc tggccatgga cgcctgctcc
     1861 ctgctggacg agaccccgcc tcagagcccc acgcgggcac tgccccggcc cctgcacccc
     1921 acgcctgtgg tggactggga cgcacgcccg ctgccccccc cgcccgccta tgacgacgtg
     1981 gcccaggatg aggatgactt tgagatctgc tccatcaaca gcaccctcgt gggcgcgggg
     2041 gtccctgccg ggcccagcca gggccagacc aactacgcct ttgtgcctga gcaggcgcgg
     2101 ccgccccctc ccctggagga caacctgttc ctcccgcccc agggtggggg caagccgccc
     2161 agctccgcac agaccgcaga gatcttccag gcgctacagc aggagtgcat gaggcaactg
     2221 caggctccgg ccggctcccc ggccccctct cccagcccgg ggggtgacga caagccccag
     2281 gtgcctcctc gggtacccat cccccctcgg cccacgcgcc cacacgtcca gctgtctcca
     2341 gcccccccgg gcgaggagga gaccagccag tggcctggac ctgcttcccc tccccgggtg
     2401 cctccgcggg agcccctgtc ccctcaaggc tcgaggacac ccagccccct ggtaccacct
     2461 ggcagctccc cgctgccacc ccggctctca agctcacctg ggaagaccat gcccaccacc
     2521 cagagctttg cctcagaccc caagtacgcc accccccagg tgatccaggc ccctggcccg
     2581 cgggctggtc cctgcatcct gcccatcgtc cgggatggca agaaggtcag cagcacccac
     2641 tattacttgc tgcccgagcg accatcctac ctggagcgct accagcgctt cctgcgtgag
     2701 gcccagagcc ccgaggagcc tacccccctg cctgtgcctc tgctgctgcc cccacccagc
     2761 accccagccc ccgccgcccc cacggccacc gtgcggccga tgccccaggc tgccttggac
     2821 cccaaggcca acttctccac caacaacagc aacccagggg cccggccacc acccccgagg
     2881 gccactgctc ggctgccaca gaggggctgc cctggcgatg ggccagaggc gggccggcca
     2941 gcagacaaga tccagatggt ggagcagctc ttcgggctgg gtctgcggcc cagaggggag
     3001 tgccacaaag tgctggagat gttcgactgg aacctggagc aggccggctg ccaccttctg
     3061 ggctcctggg gccctgccca ccacaagcgc tgagatgcgt ctggagagcc agagggcctg
     3121 cctgaaggaa tcacctgagc ctgtccgtcc accaggagtg gggagatgcc cccatccagt
     3181 cctggaggac ccgctgctcc tgctgctccc ggggatggag caaggccaag gctgcgggag
     3241 gctgggagcc ctgccctgcc catccctcct gcaccagcgc tgtccctgca cactttggtt
     3301 cagtcccggt gcccctgcca agatgtggaa ggggccgggt gaagacaggc ttgagggctg
     3361 ccccagcagg ctctgggtat gacctgcctc tggccctggt cctgggcggg gcctgtgggt
     3421 ggagtagtac ccccaggccc tgccctgggt gacagactgg gaggaaacca ggctggacct
     3481 gggcaggcgg gatgtgttgg ccacagggag aggcggaccg gcacccggtg ggacctccta
     3541 ggactgggcc ttcttccagg gggcccctgg cagcagctgg ggtgtcgggc agaatgtgac
     3601 ttgtggcctt accatggact tgaatgggac ttggctggcc tcaggatctt gtgcctggaa
     3661 atagcctgag gtggctcagg aagcggagaa agggtgccag accattctct ggcggggacc
     3721 agggcccaag gccccagggc tggaaggaga ccaaggggca gccgccctgg agggacatca
     3781 gtgcttcctc ttccacccaa ttcccccacg cggttccatg ttttcccacc agcctgttgg
     3841 cgaagttgct gctccggcat tcagtacctg cttcttccag agaaataaag ttagtttcta
     3901 ttttatgtta
//
