LOCUS       NM_001382273            4070 bp    mRNA    linear   PRI 13-MAY-2024
DEFINITION  Homo sapiens tyrosine kinase non receptor 2 (TNK2), transcript
            variant 6, mRNA.
ACCESSION   NM_001382273 XM_005269270
VERSION     NM_001382273.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Zheng,D., Wei,Z., Zhang,C., Liu,W., Gong,C., Wu,F. and Guo,W.
  TITLE     ZNF692 promotes osteosarcoma cell proliferation, migration, and
            invasion through TNK2-mediated activation of the MEK/ERK pathway
  JOURNAL   Biol Direct 19 (1), 28 (2024)
   PUBMED   38650011
  REMARK    GeneRIF: ZNF692 promotes osteosarcoma cell proliferation,
            migration, and invasion through TNK2-mediated activation of the
            MEK/ERK pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Hodder,S., Fox,M., Binti Ahmad Mokhtar,A.M., Mott,H.R. and Owen,D.
  TITLE     ACKnowledging the role of the Activated-Cdc42 associated kinase
            (ACK) in regulating protein stability in cancer
  JOURNAL   Small GTPases 14 (1), 14-25 (2023)
   PUBMED   37194323
  REMARK    GeneRIF: ACKnowledging the role of the Activated-Cdc42 associated
            kinase (ACK) in regulating protein stability in cancer.
            Review article
REFERENCE   3  (bases 1 to 4070)
  AUTHORS   Zhou,A., Zhang,W. and Wang,B.
  TITLE     Host factor TNK2 is required for influenza virus infection
  JOURNAL   Genes Genomics 45 (6), 771-781 (2023)
   PUBMED   37133719
  REMARK    GeneRIF: Host factor TNK2 is required for influenza virus
            infection.
REFERENCE   4  (bases 1 to 4070)
  AUTHORS   Kan,Y., Paung,Y., Kim,Y., Seeliger,M.A. and Miller,W.T.
  TITLE     Biochemical Studies of Systemic Lupus Erythematosus-Associated
            Mutations in Nonreceptor Tyrosine Kinases Ack1 and Brk
  JOURNAL   Biochemistry 62 (6), 1124-1137 (2023)
   PUBMED   36854171
  REMARK    GeneRIF: Biochemical Studies of Systemic Lupus
            Erythematosus-Associated Mutations in Nonreceptor Tyrosine Kinases
            Ack1 and Brk.
REFERENCE   5  (bases 1 to 4070)
  AUTHORS   Kan,Y., Paung,Y., Seeliger,M.A. and Miller,W.T.
  TITLE     Domain Architecture of the Nonreceptor Tyrosine Kinase Ack1
  JOURNAL   Cells 12 (6), 900 (2023)
   PUBMED   36980241
  REMARK    GeneRIF: Domain Architecture of the Nonreceptor Tyrosine Kinase
            Ack1.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4070)
  AUTHORS   Kato,J., Kaziro,Y. and Satoh,T.
  TITLE     Activation of the guanine nucleotide exchange factor Dbl following
            ACK1-dependent tyrosine phosphorylation
  JOURNAL   Biochem Biophys Res Commun 268 (1), 141-147 (2000)
   PUBMED   10652228
REFERENCE   7  (bases 1 to 4070)
  AUTHORS   Eisenmann,K.M., McCarthy,J.B., Simpson,M.A., Keely,P.J., Guan,J.L.,
            Tachibana,K., Lim,L., Manser,E., Furcht,L.T. and Iida,J.
  TITLE     Melanoma chondroitin sulphate proteoglycan regulates cell spreading
            through Cdc42, Ack-1 and p130cas
  JOURNAL   Nat Cell Biol 1 (8), 507-513 (1999)
   PUBMED   10587647
REFERENCE   8  (bases 1 to 4070)
  AUTHORS   Mott,H.R., Owen,D., Nietlispach,D., Lowe,P.N., Manser,E., Lim,L.
            and Laue,E.D.
  TITLE     Structure of the small G protein Cdc42 bound to the GTPase-binding
            domain of ACK
  JOURNAL   Nature 399 (6734), 384-388 (1999)
   PUBMED   10360579
REFERENCE   9  (bases 1 to 4070)
  AUTHORS   Satoh,T., Kato,J., Nishida,K. and Kaziro,Y.
  TITLE     Tyrosine phosphorylation of ACK in response to temperature
            shift-down, hyperosmotic shock, and epidermal growth factor
            stimulation
  JOURNAL   FEBS Lett 386 (2-3), 230-234 (1996)
   PUBMED   8647288
REFERENCE   10 (bases 1 to 4070)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Tan,L. and Lim,L.
  TITLE     A non-receptor tyrosine kinase that inhibits the GTPase activity of
            p21cdc42
  JOURNAL   Nature 363 (6427), 364-367 (1993)
   PUBMED   8497321
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124944.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 27, 2020 this sequence version replaced XM_005269270.3.
            
            Summary: This gene encodes a tyrosine kinase that binds Cdc42Hs in
            its GTP-bound form and inhibits both the intrinsic and
            GTPase-activating protein (GAP)-stimulated GTPase activity of
            Cdc42Hs. This binding is mediated by a unique sequence of 47 amino
            acids C-terminal to an SH3 domain. The protein may be involved in a
            regulatory mechanism that sustains the GTP-bound active form of
            Cdc42Hs and which is directly linked to a tyrosine phosphorylation
            signal transduction pathway. Several alternatively spliced
            transcript variants have been identified from this gene, but the
            full-length nature of only two transcript variants has been
            determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000672887.2/ ENSP00000499899.1
            RefSeq Select criteria :: based on manual assertion
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AC124944.8         38852-38918
            68-248              AC124944.8         58797-58977
            249-319             AC124944.8         60356-60426
            320-541             AC124944.8         62370-62591
            542-694             AC124944.8         64094-64246
            695-972             AC124944.8         65075-65352
            973-1099            AC124944.8         68228-68354
            1100-1246           AC124944.8         68811-68957
            1247-1341           AC124944.8         69056-69150
            1342-1536           AC124944.8         74933-75127
            1537-1628           AC124944.8         77198-77289
            1629-1673           AC124944.8         77862-77906
            1674-3022           AC124944.8         78694-80042
            3023-3118           AC124944.8         80139-80234
            3119-3246           AC124944.8         80387-80514
            3247-4070           AC124944.8         83216-84039
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..4070
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="tyrosine kinase non receptor 2"
                     /db_xref="GeneID:10188"
                     /db_xref="HGNC:HGNC:19297"
                     /db_xref="MIM:606994"
     exon            1..67
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            68..248
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..3253
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="isoform 6 is encoded by transcript variant 6;
                     activated p21cdc42Hs kinase; activated Cdc42-associated
                     kinase 1; tyrosine kinase non-receptor protein 2"
                     /codon_start=1
                     /product="activated CDC42 kinase 1 isoform 6"
                     /protein_id="NP_001369202.1"
                     /db_xref="CCDS:CCDS93442.1"
                     /db_xref="GeneID:10188"
                     /db_xref="HGNC:HGNC:19297"
                     /db_xref="MIM:606994"
                     /translation="MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNE
                     DLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTS
                     PAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCL
                     KPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLR
                     KHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRA
                     LPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGL
                     NGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTD
                     MRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLS
                     AQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPPQ
                     HLGGVKREPPPRPPQPAFFTQKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKP
                     SARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPT
                     RALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQ
                     TNYAFVPEQARPPPPLEDNLFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQAPAGSP
                     APSPSPGGDDKPQVPPRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPASPPRVPPREP
                     LSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAG
                     PCILPIVRDGKKVSSTHYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPST
                     PAPAAPTATVRPMPQAALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGR
                     PADKIQMLQAMVHGVTTEECQAALQCHGWSVQRAAQYLKVEQLFGLGLRPRGECHKVL
                     EMFDWNLEQAGCHLLGSWGPAHHKR"
     exon            249..319
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            320..541
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            542..694
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            695..972
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1099
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1246
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1341
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1536
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1628
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1673
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..3022
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3118
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            3119..3246
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..4070
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4044..4049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="hexamer: AATAAA"
     polyA_site      4070
                     /gene="TNK2"
                     /gene_synonym="ACK; ACK-1; ACK1; p21cdc42Hs"
                     /note="major polyA site"
ORIGIN      
        1 ctcttctgcc gtctcagcct gggcgtcgct gaagctgtgt ctgcggggag gcagcggaag
       61 gcggcagagg ctgggaggcg gcagaatgca gccagaggag ggcacaggct ggctgctgga
      121 gctgctgtcc gaggtgcagc tgcaacagta cttcctgcgg ctccgagatg acctcaacgt
      181 cacccgcctg tcccactttg agtacgtcaa gaatgaggac ctggagaaga tcggcatggg
      241 tcggcctggc cagcggcggc tgtgggaggc tgtgaagagg aggaaggcct tgtgcaaacg
      301 caagtcgtgg atgagtaagg tgttcagtgg aaagcgactg gaggctgagt tcccacctca
      361 tcactctcag agcaccttcc ggaagacctc gcccgcccct gggggcccag caggggaggg
      421 gcccctgcag agcctcacct gcctcattgg ggagaaggac ctgcgcctcc tggagaagct
      481 gggtgatggt tcctttggcg tggtgcgcag gggcgagtgg gacgcgccct cagggaagac
      541 ggtgagtgtg gctgtgaagt gcctgaagcc cgatgtcctg agccagccag aagccatgga
      601 cgacttcatc cgggaggtca atgccatgca ctcgctcgac caccgaaacc tcatccgcct
      661 ctacggggtg gtgctcacgc cgcccatgaa gatggtgaca gagctggcac ctctgggatc
      721 gttgttggac cggctacgta agcaccaggg ccacttcctc ctggggactc tgagccgcta
      781 cgctgtgcag gtggctgagg gcatgggcta cctggagtcc aagcgcttta ttcaccgtga
      841 cctggctgcc cgcaatctgc tgttggctac ccgcgacctg gtcaagatcg gggactttgg
      901 gctgatgcga gcactacctc agaatgacga ccattacgtc atgcaggaac atcgcaaggt
      961 gcccttcgcc tggtgtgccc ccgagagcct gaagacacgc accttctccc atgccagcga
     1021 cacctggatg ttcggggtga cactgtggga aatgttcacc tacggccagg agccctggat
     1081 cggcctcaac ggcagtcaga tcctgcataa gatcgacaag gagggggagc ggctgccccg
     1141 gcccgaggac tgtccccagg acatctacaa cgtcatggtc cagtgctggg ctcacaagcc
     1201 agaggacaga cccacgtttg tggccctgcg ggacttcctg ctggaggccc agcccacaga
     1261 catgcgggcc cttcaggact ttgaggaacc ggacaagctg cacatccaga tgaatgatgt
     1321 catcaccgtc atcgagggaa gggccgagaa ctactggtgg cgtggccaga acacacggac
     1381 gctgtgtgtg gggcccttcc ctcgcaacgt ggtgacctcc gtggccggcc tgtcggccca
     1441 ggacatcagc cagcccctgc agaacagctt catccacaca gggcatggcg acagtgaccc
     1501 ccgccactgc tggggcttcc cggacaggat tgacgaactg tatctgggaa accccatgga
     1561 cccccccgac ctcctgagcg tggaactgag cacctcccgg cccccccagc atctaggagg
     1621 ggtgaaaagg gagcctccac ctcgcccacc tcagcctgcc ttcttcactc agaaaccaac
     1681 ctatgaccct gtgagcgagg accaagaccc cttgtccagc gacttcaaga ggctgggcct
     1741 gcggaagcca ggcctgcccc gagggctgtg gctggcgaag ccctcggcgc gggtgccggg
     1801 caccaaggcc agccgaggca gcggggctga ggtcacgctc atcgacttcg gtgaggagcc
     1861 cgtggtcccg gccctacggc cctgcgcgcc ctccctggcg cagctggcca tggacgcctg
     1921 ctccctgctg gacgagaccc cgcctcagag ccccacgcgg gcactgcccc ggcccctgca
     1981 ccccacgcct gtggtggact gggacgcacg cccgctgccc cccccgcccg cctatgacga
     2041 cgtggcccag gatgaggatg actttgagat ctgctccatc aacagcaccc tcgtgggcgc
     2101 gggggtccct gccgggccca gccagggcca gaccaactac gcctttgtgc ctgagcaggc
     2161 gcggccgccc cctcccctgg aggacaacct gttcctcccg ccccagggtg ggggcaagcc
     2221 gcccagctcc gcacagaccg cagagatctt ccaggcgcta cagcaggagt gcatgaggca
     2281 actgcaggct ccggccggct ccccggcccc ctctcccagc ccggggggtg acgacaagcc
     2341 ccaggtgcct cctcgggtac ccatcccccc tcggcccacg cgcccacacg tccagctgtc
     2401 tccagccccc ccgggcgagg aggagaccag ccagtggcct ggacctgctt cccctccccg
     2461 ggtgcctccg cgggagcccc tgtcccctca aggctcgagg acacccagcc ccctggtacc
     2521 acctggcagc tccccgctgc caccccggct ctcaagctca cctgggaaga ccatgcccac
     2581 cacccagagc tttgcctcag accccaagta cgccaccccc caggtgatcc aggcccctgg
     2641 cccgcgggct ggtccctgca tcctgcccat cgtccgggat ggcaagaagg tcagcagcac
     2701 ccactattac ttgctgcccg agcgaccatc ctacctggag cgctaccagc gcttcctgcg
     2761 tgaggcccag agccccgagg agcctacccc cctgcctgtg cctctgctgc tgcccccacc
     2821 cagcacccca gcccccgccg cccccacggc caccgtgcgg ccgatgcccc aggctgcctt
     2881 ggaccccaag gccaacttct ccaccaacaa cagcaaccca ggggcccggc caccaccccc
     2941 gagggccact gctcggctgc cacagagggg ctgccctggc gatgggccag aggcgggccg
     3001 gccagcagac aagatccaga tgctgcaggc catggtgcat ggggtgacca cagaggagtg
     3061 ccaggcggcc ctgcagtgcc acggctggag cgtgcagagg gctgcccagt atctgaaggt
     3121 ggagcagctc ttcgggctgg gtctgcggcc cagaggggag tgccacaaag tgctggagat
     3181 gttcgactgg aacctggagc aggccggctg ccaccttctg ggctcctggg gccctgccca
     3241 ccacaagcgc tgagatgcgt ctggagagcc agagggcctg cctgaaggaa tcacctgagc
     3301 ctgtccgtcc accaggagtg gggagatgcc cccatccagt cctggaggac ccgctgctcc
     3361 tgctgctccc ggggatggag caaggccaag gctgcgggag gctgggagcc ctgccctgcc
     3421 catccctcct gcaccagcgc tgtccctgca cactttggtt cagtcccggt gcccctgcca
     3481 agatgtggaa ggggccgggt gaagacaggc ttgagggctg ccccagcagg ctctgggtat
     3541 gacctgcctc tggccctggt cctgggcggg gcctgtgggt ggagtagtac ccccaggccc
     3601 tgccctgggt gacagactgg gaggaaacca ggctggacct gggcaggcgg gatgtgttgg
     3661 ccacagggag aggcggaccg gcacccggtg ggacctccta ggactgggcc ttcttccagg
     3721 gggcccctgg cagcagctgg ggtgtcgggc agaatgtgac ttgtggcctt accatggact
     3781 tgaatgggac ttggctggcc tcaggatctt gtgcctggaa atagcctgag gtggctcagg
     3841 aagcggagaa agggtgccag accattctct ggcggggacc agggcccaag gccccagggc
     3901 tggaaggaga ccaaggggca gccgccctgg agggacatca gtgcttcctc ttccacccaa
     3961 ttcccccacg cggttccatg ttttcccacc agcctgttgg cgaagttgct gctccggcat
     4021 tcagtacctg cttcttccag agaaataaag ttagtttcta ttttatgtta
//
