LOCUS       NM_003985               2761 bp    mRNA    linear   PRI 13-DEC-2024
DEFINITION  Homo sapiens tyrosine kinase non receptor 1 (TNK1), transcript
            variant 2, mRNA.
ACCESSION   NM_003985
VERSION     NM_003985.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Nawarathnage,S., Tseng,Y.J., Soleimani,S., Smith,T., Pedroza
            Romo,M.J., Abiodun,W.O., Egbert,C.M., Madhusanka,D., Bunn,D.,
            Woods,B., Tsubaki,E., Stewart,C., Brown,S., Doukov,T.,
            Andersen,J.L. and Moody,J.D.
  TITLE     Fusion crystallization reveals the behavior of both the 1TEL
            crystallization chaperone and the TNK1 UBA domain
  JOURNAL   Structure 31 (12), 1589-1603 (2023)
   PUBMED   37776857
  REMARK    GeneRIF: Fusion crystallization reveals the behavior of both the
            1TEL crystallization chaperone and the TNK1 UBA domain.
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 2761)
  AUTHORS   Ahmed,S. and Miller,W.T.
  TITLE     The noncatalytic regions of the tyrosine kinase Tnk1 are important
            for activity and substrate specificity
  JOURNAL   J Biol Chem 298 (12), 102664 (2022)
   PUBMED   36334623
  REMARK    GeneRIF: The noncatalytic regions of the tyrosine kinase Tnk1 are
            important for activity and substrate specificity.
REFERENCE   4  (bases 1 to 2761)
  AUTHORS   Chan,T.Y., Egbert,C.M., Maxson,J.E., Siddiqui,A., Larsen,L.J.,
            Kohler,K., Balasooriya,E.R., Pennington,K.L., Tsang,T.M., Frey,M.,
            Soderblom,E.J., Geng,H., Muschen,M., Forostyan,T.V., Free,S.,
            Mercenne,G., Banks,C.J., Valdoz,J., Whatcott,C.J., Foulks,J.M.,
            Bearss,D.J., O'Hare,T., Huang,D.C.S., Christensen,K.A., Moody,J.,
            Warner,S.L., Tyner,J.W. and Andersen,J.L.
  TITLE     TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be
            targeted to block tumor growth
  JOURNAL   Nat Commun 12 (1), 5337 (2021)
   PUBMED   34504101
  REMARK    GeneRIF: TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase
            that can be targeted to block tumor growth.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2761)
  AUTHORS   Zeman,T., Balcar,V.J., Cahova,K., Janoutova,J., Janout,V.,
            Lochman,J. and Sery,O.
  TITLE     Polymorphism rs11867353 of Tyrosine Kinase Non-Receptor 1 (TNK1)
            Gene Is a Novel Genetic Marker for Alzheimer's Disease
  JOURNAL   Mol Neurobiol 58 (3), 996-1005 (2021)
   PUBMED   33070267
  REMARK    GeneRIF: Polymorphism rs11867353 of Tyrosine Kinase Non-Receptor 1
            (TNK1) Gene Is a Novel Genetic Marker for Alzheimer's Disease.
REFERENCE   6  (bases 1 to 2761)
  AUTHORS   Schjeide,B.M., McQueen,M.B., Mullin,K., DiVito,J., Hogan,M.F.,
            Parkinson,M., Hooli,B., Lange,C., Blacker,D., Tanzi,R.E. and
            Bertram,L.
  TITLE     Assessment of Alzheimer's disease case-control associations using
            family-based methods
  JOURNAL   Neurogenetics 10 (1), 19-25 (2009)
   PUBMED   18830724
  REMARK    GeneRIF: Meta-analysis of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 2761)
  AUTHORS   Azoitei,N., Brey,A., Busch,T., Fulda,S., Adler,G. and
            Seufferlein,T.
  TITLE     Thirty-eight-negative kinase 1 (TNK1) facilitates TNFalpha-induced
            apoptosis by blocking NF-kappaB activation
  JOURNAL   Oncogene 26 (45), 6536-6545 (2007)
   PUBMED   17471239
  REMARK    GeneRIF: TNK1 therefore acts as a novel molecular switch that can
            determine the properties of TNFalpha signaling and therefore cell
            death.
REFERENCE   8  (bases 1 to 2761)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   9  (bases 1 to 2761)
  AUTHORS   Felschow,D.M., Civin,C.I. and Hoehn,G.T.
  TITLE     Characterization of the tyrosine kinase Tnk1 and its binding with
            phospholipase C-gamma1
  JOURNAL   Biochem Biophys Res Commun 273 (1), 294-301 (2000)
   PUBMED   10873601
REFERENCE   10 (bases 1 to 2761)
  AUTHORS   Hoehn,G.T., Stokland,T., Amin,S., Ramirez,M., Hawkins,A.L.,
            Griffin,C.A., Small,D. and Civin,C.I.
  TITLE     Tnk1: a novel intracellular tyrosine kinase gene isolated from
            human umbilical cord blood CD34+/Lin-/CD38- stem/progenitor cells
  JOURNAL   Oncogene 12 (4), 903-913 (1996)
   PUBMED   8632913
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC113189.11.
            
            On Aug 13, 2020 this sequence version replaced NM_003985.5.
            
            Summary: The protein encoded by this gene belongs to the tyrosine
            protein kinase family. Tyrosine protein kinases are important
            regulators of intracellular signal transduction pathways, mediating
            cellular proliferation, survival, and development. This gene is
            highly expressed in fetal tissues and at lower levels in few adult
            tissues, thus may function in signaling pathways utilized broadly
            during fetal development, and more selectively in adult tissues. It
            plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and
            knockout mice have been shown to develop spontaneous tumors,
            suggesting a role as a tumor suppressor gene. Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            acceptor splice site at an internal coding exon compared to variant
            1. This results in a shorter isoform (2) missing a 5 aa protein
            segment compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225403.1, SRR11853567.12408.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000688331.1/ ENSP00000509611.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC113189.11        31172-31197
            27-280              AC113189.11        32919-33172
            281-351             AC113189.11        33333-33403
            352-543             AC113189.11        33508-33699
            544-699             AC113189.11        33792-33947
            700-983             AC113189.11        34053-34336
            984-1254            AC113189.11        34567-34837
            1255-1349           AC113189.11        36644-36738
            1350-1514           AC113189.11        37073-37237
            1515-1594           AC113189.11        37461-37540
            1595-1893           AC113189.11        38489-38787
            1894-1989           AC113189.11        38871-38966
            1990-2761           AC113189.11        39054-39825
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..2761
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /note="tyrosine kinase non receptor 1"
                     /db_xref="GeneID:8711"
                     /db_xref="HGNC:HGNC:11940"
                     /db_xref="MIM:608076"
     exon            1..26
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            27..280
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..2103
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /EC_number="2.7.10.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     non-receptor tyrosine-protein kinase TNK1; CD38 negative
                     kinase 1"
                     /codon_start=1
                     /product="non-receptor tyrosine-protein kinase TNK1
                     isoform 2"
                     /protein_id="NP_003976.2"
                     /db_xref="CCDS:CCDS45602.1"
                     /db_xref="GeneID:8711"
                     /db_xref="HGNC:HGNC:11940"
                     /db_xref="MIM:608076"
                     /translation="MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPE
                     DLDGIGMGRPAQRRLSEALKRLRSGPKSKNWVYKILGGFAPEHKEPTLPSDSPRHLPE
                     PEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSGKSVPVAVKSLRVGPEGPMGT
                     ELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTAPAPTPPLL
                     VALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGR
                     YVMGGPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQ
                     RLEDRARLPRPPLCSRALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVT
                     EPGALRMETGDPITVIEGSPDSTIWKGQNGRTFKVGSFPASAVTLADAGGLPATRPVH
                     RGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLERMKGISRSLESVLSLGPRP
                     TGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPPHNHPMG
                     MPGARKAAALSGGLLSDPELQRKIMEVELSVHGVTHQECQTALGATGGDVVSAIRNLK
                     VDQLFHLSSRSRADCWRILEHYQWDLSAASRYVLARP"
     exon            281..351
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            352..543
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..699
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..983
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1254
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1349
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1514
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1594
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1893
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1989
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2761
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2734..2739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /note="hexamer: ATTACA"
     polyA_site      2761
                     /gene="TNK1"
                     /gene_synonym="KOS1"
                     /note="major polyA site"
ORIGIN      
        1 gcagtcgccg cttccgcctt gaccaggtgg agctggagac ctggtctctc tagggcctac
       61 cctgagctca ccatctgaag gagagtgcca tcatccttag gaactccttc tccagacatg
      121 cttcctgagg ctggctccct gtggctactg aagctgctcc gggacatcca gttggcccag
      181 ttttactggc ccatccttga ggagcttaat gtcactcggc cagagcactt cgactttgta
      241 aagcctgagg acctggacgg cattggcatg ggccggcctg cccagcgcag actgtccgaa
      301 gctctgaaaa ggctacgttc tgggcctaag tctaagaact gggtctacaa gatccttgga
      361 ggttttgccc ctgagcacaa ggagcccacc ctgccctcgg acagcccacg gcacctccct
      421 gagccagagg ggggcctcaa gtgtctgatc ccagagggtg ctgtttgcag aggggagctg
      481 ctgggttcag gctgcttcgg tgtggtgcac cgagggctgt ggacgctgcc cagtggcaag
      541 agtgtcccag tggctgtcaa gtccctccgg gtaggtcccg aaggcccgat gggcacagaa
      601 ctgggggact tcctgcgaga ggtatcggtc atgatgaact tggagcaccc acacgtgctg
      661 cgtctgcacg gccttgtact gggccagcct ctgcagatgg tgatggagct ggcgccactg
      721 ggctccctgc acgcgcgcct aacggccccg gccccgacac ccccgctgct cgtggccctg
      781 ctctgcctct tcctgcggca gctggcggga gccatggcgt acctgggggc ccgcgggctg
      841 gtgcaccgag acctcgctac gcgcaaccta ctgctggcgt cgccgcgcac catcaaggtg
      901 gctgacttcg ggctggtgcg gcctctgggc ggtgcccggg gccgctacgt catgggcggg
      961 ccccgcccta tcccctacgc ctggtgtgcc ccagagagcc tgcgccacgg agccttctcg
     1021 tctgcctcgg acgtgtggat gtttggggtg acgctgtggg agatgttctc cgggggcgag
     1081 gaaccctggg ccggggtccc accgtacctc atcctgcagc ggctggagga cagagcccgg
     1141 ctgcctaggc ctcccctctg ctccagggcc ctctactccc tcgccttgcg ctgctgggcc
     1201 ccccaccctg ccgaccggcc tagcttttcc cacctggagg ggctgctgca agaggccggg
     1261 ccttcggaag catgttgtgt gagggatgtc acagaaccag gcgccctgag gatggagact
     1321 ggtgacccca tcacagtcat cgagggcagc cccgactcca caatctggaa gggccagaat
     1381 ggtcgcacct tcaaagtggg cagcttccca gcctcggcag tgacgctggc agatgcgggg
     1441 ggcttgccag ccacccgtcc agtccacaga ggcacccctg cccggggaga tcaacaccca
     1501 ggaagcatag atggagacag aaagaaggca aatctttggg atgcgccccc agcacggggc
     1561 cagaggagga acatgcccct ggagaggatg aaaggcattt ccaggagtct ggagtcagtt
     1621 ctgtccctcg gtcctcgtcc cacagggggt ggttcaagcc cccctgaaat tcgacaagcc
     1681 agagctgtgc cccagggacc tccaggcctg cctccacgcc cacctttatc ctctagctct
     1741 cctcagccca gccagccctc tagggagagg cttccctggc ccaaaagaaa acccccacac
     1801 aatcacccca tgggaatgcc tggagcccgt aaagccgctg ccctctctgg aggcctcttg
     1861 tccgatcctg agttgcagag gaagattatg gaggtggagc tgagtgtgca tggggtcacc
     1921 caccaggagt gccagacagc actaggagcc actgggggag atgtggtttc tgccatccgg
     1981 aacctcaagg tagatcagct cttccacctg agtagccggt ccagagctga ctgctggcgc
     2041 atcctggagc attaccagtg ggacctctca gctgccagcc gctatgtcct ggccaggccc
     2101 tgagctcagc ttctgcgggc acagacacca gcatgaaaag cctaggcccc tgagggcctg
     2161 gccacatggg accaagcgga accagaacaa ggtcccgaca ggggtagacg ttccacctgg
     2221 ggagatccca cctgccgtag gcacatggag gaggagccca gagttgggca ctggcaaatg
     2281 tctcctccct cccatgctcc ttggcttctg aaggctgaag ctcctttggc tgggccaaga
     2341 aggatctagt ctgcccacta cattctcaaa caagaggact tggaagaaaa gagctgctat
     2401 acatcatatg cagaggaagc ttctacgcgc tagagaggat caaggggcca cactggacca
     2461 tgtgaacagc catcctgaac tgccatcagc taccacactg gactctgcag ggcagccatc
     2521 ctggatgatg gaagccacca tattgacttg gggtataggc ccaaactgcc ttcgtttggt
     2581 ccagggccat cgtgggtgat gacgattgct ctcttgcact caaggacatt tgatgctggt
     2641 agtatggatt atgagatgga ctagcccctg ccccagccca gctctcacat tcccctttgt
     2701 tttttcccat accaactgct tctaccctcc cctattacat acatctttca atgtccaaaa
     2761 a
//
