LOCUS       NM_019894               5516 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens transmembrane serine protease 4 (TMPRSS4), transcript
            variant 1, mRNA.
ACCESSION   NM_019894
VERSION     NM_019894.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Xu,X., Sun,T. and Jing,J.
  TITLE     TMPRSS4 is a novel biomarker and correlated with immune
            infiltration in thyroid carcinoma
  JOURNAL   BMC Endocr Disord 22 (1), 280 (2022)
   PUBMED   36380313
  REMARK    GeneRIF: TMPRSS4 is a novel biomarker and correlated with immune
            infiltration in thyroid carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Temena,M.A. and Acar,A.
  TITLE     Increased TRIM31 gene expression is positively correlated with
            SARS-CoV-2 associated genes TMPRSS2 and TMPRSS4 in gastrointestinal
            cancers
  JOURNAL   Sci Rep 12 (1), 11763 (2022)
   PUBMED   35970857
  REMARK    GeneRIF: Increased TRIM31 gene expression is positively correlated
            with SARS-CoV-2 associated genes TMPRSS2 and TMPRSS4 in
            gastrointestinal cancers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5516)
  AUTHORS   Tazawa,H., Suzuki,T., Saito,A., Ishikawa,A., Komo,T., Sada,H.,
            Shimada,N., Hadano,N., Onoe,T., Sudo,T., Shimizu,Y., Kuraoka,K. and
            Tashiro,H.
  TITLE     Utility of TMPRSS4 as a Prognostic Biomarker and Potential
            Therapeutic Target in Patients with Gastric Cancer
  JOURNAL   J Gastrointest Surg 26 (2), 305-313 (2022)
   PUBMED   34379296
  REMARK    GeneRIF: Utility of TMPRSS4 as a Prognostic Biomarker and Potential
            Therapeutic Target in Patients with Gastric Cancer.
REFERENCE   4  (bases 1 to 5516)
  AUTHORS   Zhao,X.F., Yang,Y.S., Gao,D.Z. and Park,Y.K.
  TITLE     TMPRSS4 overexpression promotes the metastasis of colorectal cancer
            and predicts poor prognosis of stage III-IV colorectal cancer
  JOURNAL   Int J Biol Markers 36 (3), 23-32 (2021)
   PUBMED   34595980
  REMARK    GeneRIF: TMPRSS4 overexpression promotes the metastasis of
            colorectal cancer and predicts poor prognosis of stage III-IV
            colorectal cancer.
REFERENCE   5  (bases 1 to 5516)
  AUTHORS   Yang,Y.S., Wen,D. and Zhao,X.F.
  TITLE     Transmembrane Protease TMPRSS4 Promotes the Formation and
            Development of Mismatch Repair Deficient Colon Cancer Liver
            Metastasis
  JOURNAL   Bull Exp Biol Med 171 (2), 242-246 (2021)
   PUBMED   34173918
  REMARK    GeneRIF: Transmembrane Protease TMPRSS4 Promotes the Formation and
            Development of Mismatch Repair Deficient Colon Cancer Liver
            Metastasis.
            Erratum:[Bull Exp Biol Med. 2021 Nov;172(1):112. doi:
            10.1007/s10517-021-05343-9.. PMID: 34796428]
REFERENCE   6  (bases 1 to 5516)
  AUTHORS   Bertram,S., Glowacka,I., Blazejewska,P., Soilleux,E., Allen,P.,
            Danisch,S., Steffen,I., Choi,S.Y., Park,Y., Schneider,H.,
            Schughart,K. and Pohlmann,S.
  TITLE     TMPRSS2 and TMPRSS4 facilitate trypsin-independent spread of
            influenza virus in Caco-2 cells
  JOURNAL   J Virol 84 (19), 10016-10025 (2010)
   PUBMED   20631123
  REMARK    GeneRIF: RNAi-mediated knockdown of TMPRSS2 and TMPRSS4 in Caco-2
            cells, which released fully infectious virus without trypsin
            treatment, markedly reduced the spread of influenza virus,
            demonstrating that these proteases were responsible for activation
            of HA.
REFERENCE   7  (bases 1 to 5516)
  AUTHORS   Kim,S., Kang,H.Y., Nam,E.H., Choi,M.S., Zhao,X.F., Hong,C.S.,
            Lee,J.W., Lee,J.H. and Park,Y.K.
  TITLE     TMPRSS4 induces invasion and epithelial-mesenchymal transition
            through upregulation of integrin alpha5 and its signaling pathways
  JOURNAL   Carcinogenesis 31 (4), 597-606 (2010)
   PUBMED   20118200
  REMARK    GeneRIF: TMPRSS4 expression was significantly higher in human
            colorectal cancer tissues from advanced stages than in that of
            early stage.
REFERENCE   8  (bases 1 to 5516)
  AUTHORS   Chaipan,C., Kobasa,D., Bertram,S., Glowacka,I., Steffen,I.,
            Tsegaye,T.S., Takeda,M., Bugge,T.H., Kim,S., Park,Y., Marzi,A. and
            Pohlmann,S.
  TITLE     Proteolytic activation of the 1918 influenza virus hemagglutinin
  JOURNAL   J Virol 83 (7), 3200-3211 (2009)
   PUBMED   19158246
  REMARK    GeneRIF: The authors provide evidence that TMPRSS2 and TMPRSS4
            activate the 1918 HA by cleavage and therefore may promote viral
            spread in lung tissue.
REFERENCE   9  (bases 1 to 5516)
  AUTHORS   Jung,H., Lee,K.P., Park,S.J., Park,J.H., Jang,Y.S., Choi,S.Y.,
            Jung,J.G., Jo,K., Park,D.Y., Yoon,J.H., Park,J.H., Lim,D.S.,
            Hong,G.R., Choi,C., Park,Y.K., Lee,J.W., Hong,H.J., Kim,S. and
            Park,Y.W.
  TITLE     TMPRSS4 promotes invasion, migration and metastasis of human tumor
            cells by facilitating an epithelial-mesenchymal transition
  JOURNAL   Oncogene 27 (18), 2635-2647 (2008)
   PUBMED   17968309
  REMARK    GeneRIF: TMPRSS4 plays a role in invasion, metastasis, migration
            and adhesion in cancer cells. TMPRSS4 may contribute to tumor cell
            invasion and metastasis by promoting an epithelial-mesenchymal
            transition(EMT) through the strong SIP1/ZEB2 induction.
REFERENCE   10 (bases 1 to 5516)
  AUTHORS   Wallrapp,C., Hahnel,S., Muller-Pillasch,F., Burghardt,B.,
            Iwamura,T., Ruthenburger,M., Lerch,M.M., Adler,G. and Gress,T.M.
  TITLE     A novel transmembrane serine protease (TMPRSS3) overexpressed in
            pancreatic cancer
  JOURNAL   Cancer Res 60 (10), 2602-2606 (2000)
   PUBMED   10825129
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000665.5 and AP002800.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_019894.3.
            
            Summary: This gene encodes a member of the serine protease family.
            Serine proteases are known to be involved in a variety of
            biological processes, whose malfunction often leads to human
            diseases and disorders. This gene was identified as a gene
            overexpressed in pancreatic carcinoma. The encoded protein is
            membrane bound with a N-terminal anchor sequence and a glycosylated
            extracellular region containing the serine protease domain. The
            protein has been found to promote SARS-CoV-2 entry into host cells.
            [provided by RefSeq, Aug 2021].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). The splice acceptor
            site used for the first intron of this variant is polymorphic in
            the human population (rs2276122), and it is not known if this
            variant can be expressed from individuals with the 'A' allele.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011703.2, AF179224.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in SARS-CoV-2 infection
            MANE Ensembl match     :: ENST00000437212.8/ ENSP00000416037.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               AP000665.5         87723-87950
            229-268             AP002800.4         17416-17455
            269-382             AP002800.4         21585-21698
            383-535             AP002800.4         25701-25853
            536-665             AP002800.4         27291-27420
            666-767             AP002800.4         30374-30475
            768-808             AP002800.4         31456-31496
            809-968             AP002800.4         34341-34500
            969-1135            AP002800.4         35869-36035
            1136-1234           AP002800.4         37429-37527
            1235-1377           AP002800.4         37738-37880
            1378-1527           AP002800.4         39905-40054
            1528-5516           AP002800.4         40502-44490
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..5516
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="transmembrane serine protease 4"
                     /db_xref="GeneID:56649"
                     /db_xref="HGNC:HGNC:11878"
                     /db_xref="MIM:606565"
     exon            1..228
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..1539
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane serine protease 3; membrane-type serine
                     protease 2; type II membrane serine protease;
                     channel-activating protease 2; transmembrane protease,
                     serine 4; channel-activating serine protease 2"
                     /codon_start=1
                     /product="transmembrane protease serine 4 isoform 1"
                     /protein_id="NP_063947.2"
                     /db_xref="CCDS:CCDS31684.1"
                     /db_xref="GeneID:56649"
                     /db_xref="HGNC:HGNC:11878"
                     /db_xref="MIM:606565"
                     /translation="MLQDPDSDQPLNSLDVKPLRKPRIPMETFRKVGIPIIIALLSLA
                     SIIIVVVLIKVILDKYYFLCGQPLHFIPRKQLCDGELDCPLGEDEEHCVKSFPEGPAV
                     AVRLSKDRSTLQVLDSATGNWFSACFDNFTEALAETACRQMGYSSKPTFRAVEIGPDQ
                     DLDVVEITENSQELRMRNSSGPCLSGSLVSLHCLACGKSLKTPRVVGVEEASVDSWPW
                     QVSIQYDKQHVCGGSILDPHWVLTAAHCFRKHTDVFNWKVRAGSDKLGSFPSLAVAKI
                     IIIEFNPMYPKDNDIALMKLQFPLTFSGTVRPICLPFFDEELTPATPLWIIGWGFTKQ
                     NGGKMSDILLQASVQVIDSTRCNADDAYQGEVTEKMMCAGIPEGGVDTCQGDSGGPLM
                     YQSDQWHVVGIVSWGYGCGGPSTPGVYTKVSAYLNWIYNVWKAEL"
     exon            229..268
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            269..382
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            383..535
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            536..665
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            666..767
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            768..808
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            809..968
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1135
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1234
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1377
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1527
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..5516
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2056..2061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="hexamer: ATTAAA"
     polyA_site      2083
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="major polyA site"
     regulatory      3443..3448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="hexamer: AATGAA"
     polyA_site      3469
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="major polyA site"
     regulatory      3824..3829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
                     /note="hexamer: AATAAT"
     polyA_site      3848
                     /gene="TMPRSS4"
                     /gene_synonym="CAP2; CAPH2; MT-SP2; TMPRSS3"
ORIGIN      
        1 actcctggaa tacacagaga gaggcagcag cttgctcagc ggacaaggat gctgggcgtg
       61 agggaccaag gcctgccctg cactcgggcc tcctccagcc agtgctgacc agggacttct
      121 gacctgctgg ccagccagga cctgtgtggg gaggccctcc tgctgccttg gggtgacaat
      181 ctcagctcca ggctacaggg agaccgggag gatcacagag ccagcatgtt acaggatcct
      241 gacagtgatc aacctctgaa cagcctcgat gtcaaacccc tgcgcaaacc ccgtatcccc
      301 atggagacct tcagaaaggt ggggatcccc atcatcatag cactactgag cctggcgagt
      361 atcatcattg tggttgtcct catcaaggtg attctggata aatactactt cctctgcggg
      421 cagcctctcc acttcatccc gaggaagcag ctgtgtgacg gagagctgga ctgtcccttg
      481 ggggaggacg aggagcactg tgtcaagagc ttccccgaag ggcctgcagt ggcagtccgc
      541 ctctccaagg accgatccac actgcaggtg ctggactcgg ccacagggaa ctggttctct
      601 gcctgtttcg acaacttcac agaagctctc gctgagacag cctgtaggca gatgggctac
      661 agcagcaaac ccactttcag agctgtggag attggcccag accaggatct ggatgttgtt
      721 gaaatcacag aaaacagcca ggagcttcgc atgcggaact caagtgggcc ctgtctctca
      781 ggctccctgg tctccctgca ctgtcttgcc tgtgggaaga gcctgaagac cccccgtgtg
      841 gtgggtgtgg aggaggcctc tgtggattct tggccttggc aggtcagcat ccagtacgac
      901 aaacagcacg tctgtggagg gagcatcctg gacccccact gggtcctcac ggcagcccac
      961 tgcttcagga aacataccga tgtgttcaac tggaaggtgc gggcaggctc agacaaactg
     1021 ggcagcttcc catccctggc tgtggccaag atcatcatca ttgaattcaa ccccatgtac
     1081 cccaaagaca atgacatcgc cctcatgaag ctgcagttcc cactcacttt ctcaggcaca
     1141 gtcaggccca tctgtctgcc cttctttgat gaggagctca ctccagccac cccactctgg
     1201 atcattggat ggggctttac gaagcagaat ggagggaaga tgtctgacat actgctgcag
     1261 gcgtcagtcc aggtcattga cagcacacgg tgcaatgcag acgatgcgta ccagggggaa
     1321 gtcaccgaga agatgatgtg tgcaggcatc ccggaagggg gtgtggacac ctgccagggt
     1381 gacagtggtg ggcccctgat gtaccaatct gaccagtggc atgtggtggg catcgttagt
     1441 tggggctatg gctgcggggg cccgagcacc ccaggagtat acaccaaggt ctcagcctat
     1501 ctcaactgga tctacaatgt ctggaaggct gagctgtaat gctgctgccc ctttgcagtg
     1561 ctgggagccg cttccttcct gccctgccca cctggggatc ccccaaagtc agacacagag
     1621 caagagtccc cttgggtaca cccctctgcc cacagcctca gcatttcttg gagcagcaaa
     1681 gggcctcaat tcctataaga gaccctcgca gcccagaggc gcccagagga agtcagcagc
     1741 cctagctcgg ccacacttgg tgctcccagc atcccaggga gagacacagc ccactgaaca
     1801 aggtctcagg ggtattgcta agccaagaag gaactttccc acactactga atggaagcag
     1861 gctgtcttgt aaaagcccag atcactgtgg gctggagagg agaaggaaag ggtctgcgcc
     1921 agccctgtcc gtcttcaccc atccccaagc ctactagagc aagaaaccag ttgtaatata
     1981 aaatgcactg ccctactgtt ggtatgacta ccgttaccta ctgttgtcat tgttattaca
     2041 gctatggcca ctattattaa agagctgtgt aacatctctg gcataggcta gctggaatgc
     2101 ttgataagaa ctgagctggg atgattgaac tttcattctt tggcttgggg agaaaagaag
     2161 tcctggggaa gcaattgagt ctcaaagtag aggcagggga aaaaagagtt agggagacca
     2221 gatctgctga gtggcagcaa gagtgagctg cagattacag aaaccagggt gagcaagttt
     2281 gagtcccaca cagggccttc tccctttgcc tctttccctc cctccctgcc tgtgataatc
     2341 agccaggagc cagggataac ctatgacttg ggaaagagat gagttaggca gtcaagggtg
     2401 acattcaatc agggatccac aagtggctgg aaagaaatgc tggtcctgtg tcctaacttt
     2461 ttccgcctgg agagccctca gtgtggcttc ttacatttaa aaaacaaaaa ggatcagctg
     2521 ccaggtgtga ggcagtcccc aagctgagtt gtgaggatgt aagcatgaat aagtccctgc
     2581 actcaaaatg gtcaaagaat taaaccccat ggactttttt ggcatctgta tgaaagcttg
     2641 ggttttctga ggactgtctt gctatagtta agtcagatcc tagatgaaat atacttgttc
     2701 atactgtact aggttcttag gaaacaacag aattcctcaa atgccaaaaa caaagaaaat
     2761 agaaacccag aaaacaaaac aaaataaaac aaaaccatca gaactgtgag tggaaactaa
     2821 ggtgatgatc tgggagcaat acactaaaat cttgggtcga gacctatatg aaggctggca
     2881 gtggagctaa acctggacac actgaagaca agggagctga accagggctc ctacatgaag
     2941 cagggataac tgatggcagt aaatgtggtc tcaaattgca gatggtctgg aggaaaattt
     3001 cccaaattta gagcctcagg attcccaaag atcctccaaa tatgagctca caatcaaaga
     3061 tcagagacgt tgaaaaataa aaaacacctt aagtgggcag cataaaaaac agctaattta
     3121 gaaccccaaa ggcttcagat gtcagaatat tagagactta tgataataag caatatttgc
     3181 agagtatttg tatgtgccag acactattgt aagtgcttca tcatgtactg attcatttaa
     3241 tactcacaga aatctgtgag atgggtatta ttcttatcct cactctatgg attaaaaaaa
     3301 ctaaggcaca aagtggttaa gctccttgcc tgagattata gactgtaagt tgaacgtgag
     3361 cacttggaat acagagttca tgctgtaaac taccacacta tagggcctcc aatatgataa
     3421 tttataaaat atttgaataa aaaatgaata ctagttccac attttaaaat catgtttaac
     3481 tgtggtcaaa tgcacataac acaagttgcc atcttcacca tttttaggtg tatagttcag
     3541 tggtgttatg tacattcaca ctattgtgca gtcatcacca ccatccatct ccagaacaga
     3601 aactcagtac ccatcaaaca actctccatt tccccctcct cccaatctct ggcaaccacc
     3661 attgtgcttt cagtctctgt gaactggatt actctgggta cctcatttaa gtgaagtcat
     3721 gcagtattgg tctttttgta cttgttttat ttcacttcac attgtgtctt caagtttcac
     3781 ccatgttgta gcatgtgtca gaatttcttc cctttttaga ctaaataata ttctattgtt
     3841 tatacgaaca ttcaggttac ttctatcttt tggctattgt gaattatgct gctgtgaaca
     3901 tgggtgtaca agtatctctt tgaggccctg ctttcaattc tcttgggtat attcccagaa
     3961 gtggaattgc tggatcatat ggtaattcta ttttgaattt tttgaggaac tgatatattg
     4021 ctttccatag agactgcacc attttacatt cccatcaaca gtttgcagga gttactattt
     4081 ctccatatcc cccctaacac ttgctatttt ctgttaaaaa tggatatctt aataatcaag
     4141 caaaaataac aggcagattt gaaaaagaac tgaatacagc ttttagaaat aaaaactata
     4201 attataaaaa taaaaaacta agtggatggg gtaaataaca attaaaacac caattaagag
     4261 agaacaaatg aactggaaga taaattgaag aagtgactag gcttaacagc agagagagat
     4321 aaggagatta aaaatatgaa aacaaggcca ggagcaatga agcctagaat ggtaaattct
     4381 aacatatcca gaatcccaga aagagagaat caagacaatg agagagagac agtaccaaag
     4441 agataagagc tgagaatgtt ccagaattga taaaaggtgt gaatccacag aacatacacc
     4501 accatagtgt acacgcatac aaccaaggtg gaaaaattag aataaatcca cacctatgta
     4561 cattataatg aaactgcaga acaccaaaga caaaaagaaa ctccttatag cagcagagag
     4621 aaaacccaga ccacccacag taccacaaat ctaccacaat tagactgaca acaggctttc
     4681 ccacagcaat aaaggagcta gaagtcagtg gaagtatatc tccagcatgc caaaagataa
     4741 caatcaatca gggattgtga accctacaaa actatctttc aagaataaag gcattttcaa
     4801 gaaaacaaaa acagacttta ccatcaacaa accttctcta aaagaatata taaagcattt
     4861 actttaggaa gaaggaaaat gatcctaaaa ggaagaacca agaagcaagt agcaatagtg
     4921 aggcaattgt gaaaatgtag gtaagtctaa acacactctg tctacttctt cttcttcttc
     4981 ttcttcttct tcttcttatt ttgagactga gtcttgccct gtcacccaga ctggagtgca
     5041 gtggcaggat cttggctcac tgctatctcc acctcccagg ttcaagtgat tcttctgcct
     5101 cagcctcccg agtagctggg attacatgca catgccacca tatccggcta atttttgaat
     5161 ttttagtaga gatggggttt cactgtgttg gccaggccgg tctcaaactc ccgacctcaa
     5221 gtgatccccc cgcctcggcc tcccaaagtg ctgggattac aggcgtgtct acatattatt
     5281 aaaataacaa taatatttat tttgtgggtt aatttttttt gaaacagata ttgaatttat
     5341 tggttggcta tgagtagaaa aatacatcag taaagaaaaa agaccctgta tataaatata
     5401 atactagcta gttaaaattt gaccaagaag tttccattgt gggttaattt ttaaaggcct
     5461 aactgaaata tggagtaacc acagcatgca gcatgtaaat taaaggggat agctgg
//
