LOCUS       NM_005656               3450 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens transmembrane serine protease 2 (TMPRSS2), transcript
            variant 2, mRNA.
ACCESSION   NM_005656 XM_001717226 XM_001722209 XM_001726078
VERSION     NM_005656.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Bestle,D., Bittel,L., Werner,A.D., Kamper,L., Dolnik,O.,
            Krahling,V., Steinmetzer,T. and Bottcher-Friebertshauser,E.
  TITLE     Novel proteolytic activation of Ebolavirus glycoprotein GP by
            TMPRSS2 and cathepsin L at an uncharted position can compensate for
            furin cleavage
  JOURNAL   Virus Res 347, 199430 (2024)
   PUBMED   38964470
  REMARK    GeneRIF: Novel proteolytic activation of Ebolavirus glycoprotein GP
            by TMPRSS2 and cathepsin L at an uncharted position can compensate
            for furin cleavage.
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Pearson,N.M. and Novembre,J.
  TITLE     No evidence that ACE2 or TMPRSS2 drive population disparity in
            COVID risks
  JOURNAL   BMC Med 22 (1), 337 (2024)
   PUBMED   39183295
  REMARK    GeneRIF: No evidence that ACE2 or TMPRSS2 drive population
            disparity in COVID risks.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3450)
  AUTHORS   Wang,H., Liu,X., Zhang,X., Zhao,Z., Lu,Y., Pu,D., Zhang,Z.,
            Chen,J., Wang,Y., Li,M., Dong,X., Duan,Y., He,Y., Mao,Q., Guo,H.,
            Sun,H., Zhou,Y., Yang,Q., Gao,Y., Yang,X., Cao,H., Guddat,L.,
            Sun,L., Rao,Z. and Yang,H.
  TITLE     TMPRSS2 and glycan receptors synergistically facilitate coronavirus
            entry
  JOURNAL   Cell 187 (16), 4261-4271 (2024)
   PUBMED   38964329
  REMARK    GeneRIF: TMPRSS2 and glycan receptors synergistically facilitate
            coronavirus entry.
REFERENCE   4  (bases 1 to 3450)
  AUTHORS   Yunita,R., Wahyuni,A.S., Sinaga,B.Y., Yamamoto,Z., Soebandrio,A.,
            Kusumawati,R.L., Sembiring,R.J. and Pandia,P.
  TITLE     Role of ACE2 and TMPRSS2 polymorphisms on COVID-19 outcome and
            disease severity in adult patients: A prospective cohort study in a
            tertiary hospital, Indonesia
  JOURNAL   Narra J 4 (2), e919 (2024)
   PUBMED   39280326
  REMARK    GeneRIF: Role of ACE2 and TMPRSS2 polymorphisms on COVID-19 outcome
            and disease severity in adult patients: A prospective cohort study
            in a tertiary hospital, Indonesia.
REFERENCE   5  (bases 1 to 3450)
  AUTHORS   Meseldzic,N., Prnjavorac,B., Dujic,T., Malenica,M., Glamoclija,U.,
            Prnjavorac,L., Bedak,O., Imamovic Kadric,S., Marjanovic,D. and
            Bego,T.
  TITLE     Association of ACE2 and TMPRSS2 genes variants with disease
            severity and most important biomarkers in COVID-19 patients in
            Bosnia and Herzegovina
  JOURNAL   Croat Med J 65 (3), 220-231 (2024)
   PUBMED   38868968
  REMARK    GeneRIF: Association of ACE2 and TMPRSS2 genes variants with
            disease severity and most important biomarkers in COVID-19 patients
            in Bosnia and Herzegovina.
REFERENCE   6  (bases 1 to 3450)
  AUTHORS   Jacquinet,E., Rao,N.V., Rao,G.V., Zhengming,W., Albertine,K.H. and
            Hoidal,J.R.
  TITLE     Cloning and characterization of the cDNA and gene for human
            epitheliasin
  JOURNAL   Eur J Biochem 268 (9), 2687-2699 (2001)
   PUBMED   11322890
REFERENCE   7  (bases 1 to 3450)
  AUTHORS   Afar,D.E., Vivanco,I., Hubert,R.S., Kuo,J., Chen,E., Saffran,D.C.,
            Raitano,A.B. and Jakobovits,A.
  TITLE     Catalytic cleavage of the androgen-regulated TMPRSS2 protease
            results in its secretion by prostate and prostate cancer epithelia
  JOURNAL   Cancer Res 61 (4), 1686-1692 (2001)
   PUBMED   11245484
REFERENCE   8  (bases 1 to 3450)
  AUTHORS   Vaarala,M.H., Porvari,K.S., Kellokumpu,S., Kyllonen,A.P. and
            Vihko,P.T.
  TITLE     Expression of transmembrane serine protease TMPRSS2 in mouse and
            human tissues
  JOURNAL   J Pathol 193 (1), 134-140 (2001)
   PUBMED   11169526
REFERENCE   9  (bases 1 to 3450)
  AUTHORS   Lin,B., Ferguson,C., White,J.T., Wang,S., Vessella,R., True,L.D.,
            Hood,L. and Nelson,P.S.
  TITLE     Prostate-localized and androgen-regulated expression of the
            membrane-bound serine protease TMPRSS2
  JOURNAL   Cancer Res 59 (17), 4180-4184 (1999)
   PUBMED   10485450
REFERENCE   10 (bases 1 to 3450)
  AUTHORS   Paoloni-Giacobino,A., Chen,H., Peitsch,M.C., Rossier,C. and
            Antonarakis,S.E.
  TITLE     Cloning of the TMPRSS2 gene, which encodes a novel serine protease
            with transmembrane, LDLRA, and SRCR domains and maps to 21q22.3
  JOURNAL   Genomics 44 (3), 309-320 (1997)
   PUBMED   9325052
  REMARK    Erratum:[Genomics 2001 Sep;77(1-2):114]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP276431.1, AF270487.1,
            BC051839.1 and AP001610.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 26, 2019 this sequence version replaced NM_005656.3.
            
            Summary: This gene encodes a protein that belongs to the serine
            protease family. The encoded protein contains a type II
            transmembrane domain, a receptor class A domain, a scavenger
            receptor cysteine-rich domain and a protease domain. Serine
            proteases are known to be involved in many physiological and
            pathological processes. This gene was demonstrated to be
            up-regulated by androgenic hormones in prostate cancer cells and
            down-regulated in androgen-independent prostate cancer tissue. The
            protease domain of this protein is thought to be cleaved and
            secreted into cell media after autocleavage. This protein also
            facilitates entry of viruses into host cells by proteolytically
            cleaving and activating viral envelope glycoproteins. Viruses found
            to use this protein for cell entry include Influenza virus and the
            human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2
            (COVID-19 virus). Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Apr 2020].
            
            Transcript Variant: This variant (2) has an alternate 5' exon and
            uses a downstream AUG start codon, as compared to variant 1. The
            resulting isoform (2) has a shorter N-terminus, as compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051839.1, AF329454.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2155984 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in SARS-CoV-2 infection
            MANE Ensembl match     :: ENST00000332149.10/ ENSP00000330330.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                BP276431.1         1-21
            22-1746             AF270487.1         1-1725
            1747-2930           BC051839.1         1718-2901
            2931-3450           AP001610.1         30847-31366         c
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..3450
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /note="transmembrane serine protease 2"
                     /db_xref="GeneID:7113"
                     /db_xref="HGNC:HGNC:11876"
                     /db_xref="MIM:602060"
     exon            1..78
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            79..149
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..1613
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /EC_number="3.4.21.122"
                     /note="isoform 2 is encoded by transcript variant 2;
                     epitheliasin; serine protease 10; transmembrane protease,
                     serine 2"
                     /codon_start=1
                     /product="transmembrane protease serine 2 isoform 2"
                     /protein_id="NP_005647.3"
                     /db_xref="CCDS:CCDS33564.1"
                     /db_xref="GeneID:7113"
                     /db_xref="HGNC:HGNC:11876"
                     /db_xref="MIM:602060"
                     /translation="MALNSGSPPAIGPYYENHGYQPENPYPAQPTVVPTVYEVHPAQY
                     YPSPVPQYAPRVLTQASNPVVCTQPKSPSGTVCTSKTKKALCITLTLGTFLVGAALAA
                     GLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQV
                     YSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGN
                     VDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQ
                     NVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISH
                     PNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGK
                     TSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKN
                     NIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG"
     exon            150..372
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            373..459
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            460..579
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            580..706
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            707..817
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            818..861
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1033
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1209
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1305
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1448
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1601
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..3450
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1724..1729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /note="hexamer: ATTAAA"
     polyA_site      1746
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
     regulatory      3174..3179
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /note="hexamer: AATAAA"
     polyA_site      3204
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /note="major polyA site"
     regulatory      3422..3427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
                     /note="hexamer: AATAAA"
     polyA_site      3450
                     /gene="TMPRSS2"
                     /gene_synonym="PRSS10"
ORIGIN      
        1 gagtaggcgc gagctaagca ggaggcggag gcggaggcgg agggcgaggg gcggggagcg
       61 ccgcctggag cgcggcaggt catattgaac attccagata cctatcatta ctcgatgctg
      121 ttgataacag caagatggct ttgaactcag ggtcaccacc agctattgga ccttactatg
      181 aaaaccatgg ataccaaccg gaaaacccct atcccgcaca gcccactgtg gtccccactg
      241 tctacgaggt gcatccggct cagtactacc cgtcccccgt gccccagtac gccccgaggg
      301 tcctgacgca ggcttccaac cccgtcgtct gcacgcagcc caaatcccca tccgggacag
      361 tgtgcacctc aaagactaag aaagcactgt gcatcacctt gaccctgggg accttcctcg
      421 tgggagctgc gctggccgct ggcctactct ggaagttcat gggcagcaag tgctccaact
      481 ctgggataga gtgcgactcc tcaggtacct gcatcaaccc ctctaactgg tgtgatggcg
      541 tgtcacactg ccccggcggg gaggacgaga atcggtgtgt tcgcctctac ggaccaaact
      601 tcatccttca ggtgtactca tctcagagga agtcctggca ccctgtgtgc caagacgact
      661 ggaacgagaa ctacgggcgg gcggcctgca gggacatggg ctataagaat aatttttact
      721 ctagccaagg aatagtggat gacagcggat ccaccagctt tatgaaactg aacacaagtg
      781 ccggcaatgt cgatatctat aaaaaactgt accacagtga tgcctgttct tcaaaagcag
      841 tggtttcttt acgctgtata gcctgcgggg tcaacttgaa ctcaagccgc cagagcagga
      901 ttgtgggcgg cgagagcgcg ctcccggggg cctggccctg gcaggtcagc ctgcacgtcc
      961 agaacgtcca cgtgtgcgga ggctccatca tcacccccga gtggatcgtg acagccgccc
     1021 actgcgtgga aaaacctctt aacaatccat ggcattggac ggcatttgcg gggattttga
     1081 gacaatcttt catgttctat ggagccggat accaagtaga aaaagtgatt tctcatccaa
     1141 attatgactc caagaccaag aacaatgaca ttgcgctgat gaagctgcag aagcctctga
     1201 ctttcaacga cctagtgaaa ccagtgtgtc tgcccaaccc aggcatgatg ctgcagccag
     1261 aacagctctg ctggatttcc gggtgggggg ccaccgagga gaaagggaag acctcagaag
     1321 tgctgaacgc tgccaaggtg cttctcattg agacacagag atgcaacagc agatatgtct
     1381 atgacaacct gatcacacca gccatgatct gtgccggctt cctgcagggg aacgtcgatt
     1441 cttgccaggg tgacagtgga gggcctctgg tcacttcgaa gaacaatatc tggtggctga
     1501 taggggatac aagctggggt tctggctgtg ccaaagctta cagaccagga gtgtacggga
     1561 atgtgatggt attcacggac tggatttatc gacaaatgag ggcagacggc taatccacat
     1621 ggtcttcgtc cttgacgtcg ttttacaaga aaacaatggg gctggttttg cttccccgtg
     1681 catgatttac tcttagagat gattcagagg tcacttcatt tttattaaac agtgaacttg
     1741 tctggctttg gcactctctg ccattctgtg caggctgcag tggctcccct gcccagcctg
     1801 ctctccctaa ccccttgtcc gcaaggggtg atggccggct ggttgtgggc actggcggtc
     1861 aagtgtggag gagaggggtg gaggctgccc cattgagatc ttcctgctga gtcctttcca
     1921 ggggccaatt ttggatgagc atggagctgt cacctctcag ctgctggatg acttgagatg
     1981 aaaaaggaga gacatggaaa gggagacagc caggtggcac ctgcagcggc tgccctctgg
     2041 ggccacttgg tagtgtcccc agcctacctc tccacaaggg gattttgctg atgggttctt
     2101 agagccttag cagccctgga tggtggccag aaataaaggg accagccctt catgggtggt
     2161 gacgtggtag tcacttgtaa ggggaacaga aacatttttg ttcttatggg gtgagaatat
     2221 agacagtgcc cttggtgcga gggaagcaat tgaaaaggaa cttgccctga gcactcctgg
     2281 tgcaggtctc cacctgcaca ttgggtgggg ctcctgggag ggagactcag ccttcctcct
     2341 catcctccct gaccctgctc ctagcaccct ggagagtgca catgcccctt ggtcctggca
     2401 gggcgccaag tctggcacca tgttggcctc ttcaggcctg ctagtcactg gaaattgagg
     2461 tccatggggg aaatcaagga tgctcagttt aaggtacact gtttccatgt tatgtttcta
     2521 cacattgcta cctcagtgct cctggaaact tagcttttga tgtctccaag tagtccacct
     2581 tcatttaact ctttgaaact gtatcatctt tgccaagtaa gagtggtggc ctatttcagc
     2641 tgctttgaca aaatgactgg ctcctgactt aacgttctat aaatgaatgt gctgaagcaa
     2701 agtgcccatg gtggcggcga agaagagaaa gatgtgtttt gttttggact ctctgtggtc
     2761 ccttccaatg ctgtgggttt ccaaccaggg gaagggtccc ttttgcattg ccaagtgcca
     2821 taaccatgag cactactcta ccatggttct gcctcctggc caagcaggct ggtttgcaag
     2881 aatgaaatga atgattctac agctaggact taaccttgaa atggaaagtc atgcaatccc
     2941 atttgcagga tctgtctgtg cacatgcctc tgtagagagc agcattccca gggaccttgg
     3001 aaacagttgg cactgtaagg tgcttgctcc ccaagacaca tcctaaaagg tgttgtaatg
     3061 gtgaaaacgt cttccttctt tattgcccct tcttatttat gtgaacaact gtttgtcttt
     3121 ttttgtatct tttttaaact gtaaagttca attgtgaaaa tgaatatcat gcaaataaat
     3181 tatgcaattt ttttttcaaa gtaactactg catctttgaa gttctgcctg gtgagtagga
     3241 ccagcctcca tttccttata agggggtgat gttgaggctg ctggtcagag gaccaaaggt
     3301 gaggcaaggc cagacttggt gctcctgtgg ttggtgccct cagttcctgc agcctgtcct
     3361 gttggagagg tccctcaaat gactccttct tattattcta ttagtctgtt tccatgctcc
     3421 taataaagac atacccaaga ctgcaattta
//
