LOCUS       NM_001032283            4136 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens thymopoietin (TMPO), transcript variant 2, mRNA.
ACCESSION   NM_001032283
VERSION     NM_001032283.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Luo,X.J., He,M.M., Liu,J., Zheng,J.B., Wu,Q.N., Chen,Y.X., Meng,Q.,
            Luo,K.J., Chen,D.L., Xu,R.H., Zeng,Z.L., Liu,Z.X. and Luo,H.Y.
  TITLE     LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma
            progression by forming biomolecular condensates with FUS and p300
            to regulate TMPO transcription
  JOURNAL   Exp Mol Med 54 (6), 834-847 (2022)
   PUBMED   35760875
  REMARK    GeneRIF: LncRNA TMPO-AS1 promotes esophageal squamous cell
            carcinoma progression by forming biomolecular condensates with FUS
            and p300 to regulate TMPO transcription.
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Liu,X. and Shen,Z.
  TITLE     LncRNA TMPO-AS1 Aggravates the Development of Hepatocellular
            Carcinoma via miR-429/GOT1 Axis
  JOURNAL   Am J Med Sci 360 (6), 711-720 (2020)
   PUBMED   32988599
  REMARK    GeneRIF: LncRNA TMPO-AS1 Aggravates the Development of
            Hepatocellular Carcinoma via miR-429/GOT1 Axis.
REFERENCE   3  (bases 1 to 4136)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   4  (bases 1 to 4136)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 4136)
  AUTHORS   Qin,Z., Zheng,X. and Fang,Y.
  TITLE     Long noncoding RNA TMPO-AS1 promotes progression of non-small cell
            lung cancer through regulating its natural antisense transcript
            TMPO
  JOURNAL   Biochem Biophys Res Commun 516 (2), 486-493 (2019)
   PUBMED   31230752
  REMARK    GeneRIF: TMPO-AS1 contributes to lung carcinogenesis, which may be
            partially through upregulation TMPO.
REFERENCE   6  (bases 1 to 4136)
  AUTHORS   Harris,C.A., Andryuk,P.J., Cline,S., Chan,H.K., Natarajan,A.,
            Siekierka,J.J. and Goldstein,G.
  TITLE     Three distinct human thymopoietins are derived from alternatively
            spliced mRNAs
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6283-6287 (1994)
   PUBMED   7517549
REFERENCE   7  (bases 1 to 4136)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
REFERENCE   8  (bases 1 to 4136)
  AUTHORS   Heavner,G.A., Audhya,T. and Goldstein,G.
  TITLE     Peptide analogs of thymopentin distinguish distinct thymopoietin
            receptor specificities on two human T cell lines
  JOURNAL   Regul Pept 27 (2), 257-262 (1990)
   PUBMED   2158125
REFERENCE   9  (bases 1 to 4136)
  AUTHORS   Fuccello,A., Audhya,T., Talle,M.A. and Goldstein,G.
  TITLE     Immunoassay for bovine serum thymopoietin: discrimination from
            splenin by monoclonal antibodies
  JOURNAL   Arch Biochem Biophys 228 (1), 292-298 (1984)
   PUBMED   6364989
REFERENCE   10 (bases 1 to 4136)
  AUTHORS   Twomey,J.J., Goldstein,G., Lewis,V.M., Bealmear,P.M. and Good,R.A.
  TITLE     Bioassay determinations of thymopoietin and thymic hormone levels
            in human plasma
  JOURNAL   Proc Natl Acad Sci U S A 74 (6), 2541-2545 (1977)
   PUBMED   302007
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB239672.1, BC053675.1,
            AB209297.1, BQ232508.1, BG676381.1, BM802949.1, AI055921.1 and
            AA992204.1.
            
            On Dec 20, 2019 this sequence version replaced NM_001032283.2.
            
            Summary: Through alternative splicing, this gene encodes several
            distinct LEM domain containing protein isoforms. LEM domain
            proteins include inner nuclear membrane and intranuclear proteins,
            and are involved in a variety of cellular functions including gene
            expression, chromatin organization, and replication and cell cycle
            control. The encoded alpha isoform is broadly diffuse in the
            nucleus and contains a lamin binding domain, while the beta and
            gamma isoforms are localized to the nuclear membrane and contain an
            HDAC3 interaction domain. The distinct isoforms may compete with
            each other when acting to chaperone other proteins and regulate
            transcription. [provided by RefSeq, Aug 2019].
            
            Transcript Variant: This variant (2) differs at the 3' end compared
            to variant 1, resulting in a shorter isoform (beta) with a distinct
            C-terminus compared to isoform alpha.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U09087.1, BC053675.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000556029.6/ ENSP00000450627.1
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-576               DB239672.1         18-593
            577-2579            BC053675.1         540-2542
            2580-2812           AB209297.1         2206-2438
            2813-3084           BQ232508.1         73-344
            3085-3109           AB209297.1         2492-2516
            3110-3532           BG676381.1         204-626
            3533-3924           BM802949.1         384-775
            3925-4097           AI055921.1         4-176               c
            4098-4136           AA992204.1         34-72               c
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.1"
     gene            1..4136
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /note="thymopoietin"
                     /db_xref="GeneID:7112"
                     /db_xref="HGNC:HGNC:11875"
                     /db_xref="MIM:188380"
     exon            1..557
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..1643
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /note="isoform beta is encoded by transcript variant 2;
                     LEM domain containing 4; lamina-associated polypeptide 2"
                     /codon_start=1
                     /product="thymopoietin isoform beta"
                     /protein_id="NP_001027454.1"
                     /db_xref="CCDS:CCDS31879.1"
                     /db_xref="GeneID:7112"
                     /db_xref="HGNC:HGNC:11875"
                     /db_xref="MIM:188380"
                     /translation="MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQH
                     LTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKP
                     RQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSST
                     PLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVE
                     HNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVIS
                     ESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEK
                     TEERRVERDILKEMFPYEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVP
                     KYVPLADVKSEKTKKGRSIPVWIKILLFVVVAVFLFLVYQAMETNQVNPFSNFLHVDP
                     RKSN"
     exon            558..684
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            685..843
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            844..941
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1061
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1157
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1268
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1357
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..4136
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4076..4081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /note="hexamer: AATAAA"
     polyA_site      4097
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /note="major polyA site"
     regulatory      4111..4116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
                     /note="hexamer: AATAAA"
     polyA_site      4136
                     /gene="TMPO"
                     /gene_synonym="CMD1T; LAP2; LEMD4; PRO0868; TP"
ORIGIN      
        1 gaagcaggct gctcgcctcc tgcctgtagt gtgtgggctg gggttggtgc gagcttccag
       61 cttggccgca gttggttcgt agttcggctc tggggtcttt tgtgtccggg tctggcttgg
      121 ctttgtgtcc gcgagttttt gttccgctcc gcagcgctct tcccgggcag gagccgtgag
      181 gctcggaggc ggcagcgcgg tccccggcca ggagcaagcg cgccggcgtg agcggcggcg
      241 gcaaaggctg tggggagggg gcttcgcaga tccccgagat gccggagttc ctggaagacc
      301 cctcggtcct gacaaaagac aagttgaaga gtgagttggt cgccaacaat gtgacgctgc
      361 cggccgggga gcagcgcaaa gacgtgtacg tccagctcta cctgcagcac ctcacggctc
      421 gcaaccggcc gccgctcccc gccggcacca acagcaaggg gcccccggac ttctccagtg
      481 acgaagagcg cgagcccacc ccggtcctcg gctctggggc cgccgccgcg ggccggagcc
      541 gagcagccgt cggcaggaaa gccacaaaaa aaactgataa acccagacaa gaagataaag
      601 atgatctaga tgtaacagag ctcactaatg aagatctttt ggatcagctt gtgaaatacg
      661 gagtgaatcc tggtcctatt gtgggaacaa ccaggaagct atatgagaaa aagcttttga
      721 aactgaggga acaaggaaca gaatcaagat cttctactcc tctgccaaca atttcttctt
      781 cagcagaaaa tacaaggcag aatggaagta atgattctga cagatacagt gacaatgaag
      841 aagactctaa aatagagctc aagcttgaga agagagaacc actaaagggc agagcaaaga
      901 ctccagtaac actcaagcaa agaagagttg agcacaatca gagctattct caagctggaa
      961 taactgagac tgaatggaca agtggatctt caaaaggcgg acctctgcag gcattaacta
     1021 gggaatctac aagagggtca agaagaactc caaggaaaag ggtggaaact tcagaacatt
     1081 ttcgtataga tggtccagta atttcagaga gtactcccat agctgaaact ataatggctt
     1141 caagcaacga atccttagtt gtcaataggg tgactggaaa tttcaagcat gcatctccta
     1201 ttctgccaat cactgaattc tcagacatac ccagaagagc accaaagaaa ccattgacaa
     1261 gagctgaagt gggagaaaaa acagaggaaa gaagagtaga aagggatatt cttaaggaaa
     1321 tgttccccta tgaagcatct acaccaacag gaattagtgc tagttgccgc agaccaatca
     1381 aaggggctgc aggccggcca ttagaactca gtgatttcag gatggaggag tctttttcat
     1441 ctaaatatgt tcctaagtat gttcccttgg cagatgtcaa gtcagaaaag acaaaaaagg
     1501 gacgctccat tcccgtatgg ataaaaattt tgctgtttgt tgttgtggca gtttttttgt
     1561 ttttggtcta tcaagctatg gaaaccaacc aagtaaatcc cttctctaat tttcttcatg
     1621 ttgaccctag aaaatccaac tgaatggtat ctctttggca cgttcaactt ggtctcctat
     1681 tttcaataac tgttgaaaaa catttgtgta cacttgttga ctccaagaac taaaaataat
     1741 gtgatttcgc ctcaataaat gtagtatttc attgaaaagc aaacaaaata tatataaatg
     1801 gacttcatta aaatgttttt gaactttgga ctagtaggag atcactttgt gccatatgaa
     1861 taatcttttt tagctctgga actttttgta ggctttattt ttttaatgtg ggcatcttat
     1921 ttcatttttg aaaaaatgta tatgtttttt gtgtatttgg gaaacgaagg gtgaaacatg
     1981 gtagtataat gtgaagctac acatttaaat acttagaatt cttacagaaa agattttaag
     2041 aattattctc tgctgaataa aaactgcaaa tatgtgaaac ataatgaaat tcagtaagag
     2101 gaaaagtaac ttggttgtac tttttgtaac tgcaacaaag tttgatggtg tttatgagga
     2161 aaagtacagc aataatctct tctgtaacct ttattaatag taatgttgtt gtagccctat
     2221 catactcact ttttaagaca cagtatcatg aaagtcctat ttcagtaaga cccatttaca
     2281 tacagtagat ttttagcaga gatcttttag tgtaacatac atattttaga gaattgttgg
     2341 ctagctgtac atgttttgaa aagctgttta gctagctata aggctataat tggaaatttg
     2401 tattttttat ttacagcaaa acatttattc agtcatccag tttgctacca aaatatgttt
     2461 tagataagtg tgtgtatgtt tgtttagaag ttagaaattg taaacactgg tcttatgttt
     2521 catttggatt cattattgca ttgtcttgtt accagaaaca aattttgccg agcttttttt
     2581 gccctatatt tcccagcata atttgattag aaagtacaaa aagggccagg cgcggtggct
     2641 tacgcctgta atcccagcac tttgggaggc cagggcgggt ggatcacgag gtcaagagat
     2701 caagaccatc ctggccaaca tggtgaaacc ccgtctctac taaaaataca aaaaaattag
     2761 ctgggcgtga tgatgtgcgc ctgtagtcct gtctactagg gagactgagg caggagaatc
     2821 gtttgaaccc aggaggcaga ggttgcagtg agcagagatt acgtcactgc actgtagcct
     2881 ggcgacagag caagactcca tctcaaaaaa aataaaaaag tacaaaaagt acttgctttt
     2941 atattacatc ataagcagta gttaataaag ttgtatactc ttaagaggtg ggcattatga
     3001 ttatttttta tttttttatt ttttatgaga tggagtctca ctcttgtcac ccagactgta
     3061 gtgcagtggc acgatctggg ctcgatgcaa cctctgcctc ccaggttcaa gcaattttcc
     3121 tgcctcggca gagacggggt ttcaccatgt tggccaggct ggtctcgaac ccctgacctc
     3181 aagtgatcag cccacctcag cttcccaaag tgctgggatt acaggtgtga tccactgcac
     3241 ccggccggca ttatgatttt gtgtactctt gaaatggtta tctttgtgga tgattttttt
     3301 ttttaagctg aaacttacct catgaataac ttgattaaag tagtaggtga ttaaaatttc
     3361 aatagaatca aatgagacaa aaattttaaa ctgactcatt tgagtttcaa ctttacagtc
     3421 attgaccata aagcacacta aaaatgtaag ttatttttaa atacatctga aataaaaata
     3481 cttactaaaa aggaagaagc cgaagatgta tatttagacc agcacacaat tttgatttca
     3541 attagcctta ttctaatatt tagcttttag atctttcata cacattttca cgtactttgc
     3601 aattgagacc agaaagactt gtaggtcttt ctgcagaatg agtgggtcct tgcaaagtga
     3661 gtgggaaact tactcctaga tcagaaatgt ttgcctctct gagtaaaatg tttctttcag
     3721 atgagccata gagggggcac cttttactca acttttcttt gttttgaaac tttgtttccc
     3781 atactgtttt cagccttttg tttataatta gaaattgtga gaagcttcat ttagtgttta
     3841 aaaatgtggg gagataaatc agacttaaca tgtatgtaag atcaattcac ttaaaagtat
     3901 ggtccaaata gcaaaaatag gaccaggtga aacatgtagt cattttttaa aaacatgtac
     3961 ttggtctttt gtgtgtgtct gttttattcc attagaataa atgtgtcctt gatgtaaatg
     4021 caaagcattt cttcctgatt aaattgtaga tgtagacttt acaatataat tcaataataa
     4081 aaagtaatta acctctagtt ttgtcgttgc aataaatggt tttcagatag cacaaa
//
