LOCUS       NM_020683               1662 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transmembrane and immunoglobulin domain containing 3
            (TMIGD3), transcript variant 1, mRNA.
ACCESSION   NM_020683
VERSION     NM_020683.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Iyer,S.V., Ranjan,A., Elias,H.K., Parrales,A., Sasaki,H., Roy,B.C.,
            Umar,S., Tawfik,O.W. and Iwakuma,T.
  TITLE     Genome-wide RNAi screening identifies TMIGD3 isoform1 as a
            suppressor of NF-kappaB and osteosarcoma progression
  JOURNAL   Nat Commun 7, 13561 (2016)
   PUBMED   27886186
  REMARK    GeneRIF: Protein expression of TMIGD3 and A3AR is lower in human
            osteosarcoma tissues than normal tissues. Mechanistically, TMIGD3
            isoform 1 and A3AR commonly inhibit the PKA-Akt-NF-kappaB axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   3  (bases 1 to 1662)
  AUTHORS   Dougherty,C., Barucha,J., Schofield,P.R., Jacobson,K.A. and
            Liang,B.T.
  TITLE     Cardiac myocytes rendered ischemia resistant by expressing the
            human adenosine A1 or A3 receptor
  JOURNAL   FASEB J 12 (15), 1785-1792 (1998)
   PUBMED   9837869
REFERENCE   4  (bases 1 to 1662)
  AUTHORS   Palmer,T.M., Harris,C.A., Coote,J. and Stiles,G.L.
  TITLE     Induction of multiple effects on adenylyl cyclase regulation by
            chronic activation of the human A3 adenosine receptor
  JOURNAL   Mol Pharmacol 52 (4), 632-640 (1997)
   PUBMED   9380026
REFERENCE   5  (bases 1 to 1662)
  AUTHORS   Bouma,M.G., Jeunhomme,T.M., Boyle,D.L., Dentener,M.A.,
            Voitenok,N.N., van den Wildenberg,F.A. and Buurman,W.A.
  TITLE     Adenosine inhibits neutrophil degranulation in activated human
            whole blood: involvement of adenosine A2 and A3 receptors
  JOURNAL   J Immunol 158 (11), 5400-5408 (1997)
   PUBMED   9164961
REFERENCE   6  (bases 1 to 1662)
  AUTHORS   Sajjadi,F.G. and Firestein,G.S.
  TITLE     cDNA cloning and sequence analysis of the human A3 adenosine
            receptor
  JOURNAL   Biochim Biophys Acta 1179 (1), 105-107 (1993)
   PUBMED   8399349
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL390195.10 and AY358644.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020683.6.
            
            Summary: This gene encodes a transmembrane and immunoglobulin
            domain-containing protein. Alternative splicing results in multiple
            transcript variants, one of which shares its 5' terminal exon with
            that of the overlapping adenosine A3 receptor gene (GeneID:140),
            thus resulting in a fusion product. [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). It shares its 5' terminal exon with the adenosine A3 receptor
            gene (GeneID:140), and it thus produces a fusion protein that
            includes the adenosine A3 receptor protein N-terminus fused to the
            product of this gene (GeneID:57413).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358644.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369716.9/ ENSP00000358730.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AL390195.10        102531-102675       c
            146-1662            AY358644.1         1-1517
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..1662
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /note="transmembrane and immunoglobulin domain containing
                     3"
                     /db_xref="GeneID:57413"
                     /db_xref="HGNC:HGNC:51375"
     exon            1..629
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     CDS             280..1323
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /note="isoform 1 is encoded by transcript variant 1; AD026
                     protein (AD026); transmembrane domain-containing protein
                     TMIGD3"
                     /codon_start=1
                     /product="transmembrane and immunoglobulin domain
                     containing 3 isoform 1"
                     /protein_id="NP_065734.5"
                     /db_xref="CCDS:CCDS838.1"
                     /db_xref="GeneID:57413"
                     /db_xref="HGNC:HGNC:51375"
                     /translation="MPNNSTALSLANVTYITMEIFIGLCAIVGNVLVICVVKLNPSLQ
                     TTTFYFIVSLALADIAVGVLVMPLAIVVSLGITIHFYSCLFMTCLLLIFTHASIMSLL
                     AIAVDRYLRVKLTVRFRIPGLPGCILSFQLKVCFLPVMWLFILLSLALISDAMVMDEK
                     VKRSFVLDTASAICNYNAHYKNHPKYWCRGYFRDYCNIIAFSPNSTNHVALRDTGNQL
                     IVTMSCLTKEDTGWYWCGIQRDFARDDMDFTELIVTDDKGTLANDFWSGKDLSGNKTR
                     SCKAPKVVRKADRSRTSILIICILITGLGIISVISHLTKRRRSQRNRRVGNTLKPFSR
                     VLTPKEMAPTEQM"
     exon            630..736
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     exon            737..1084
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1151
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1252
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1662
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1635..1640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /note="hexamer: AATAAA"
     polyA_site      1662
                     /gene="TMIGD3"
                     /gene_synonym="AD026"
                     /note="major polyA site"
ORIGIN      
        1 agtccatata gagctgtcct acagcattct ggaaacttga ggatgtgcgg tgcataaagg
       61 ggctggaagt gacccacctg tgatgagccc tttctaagga gaagggtttc caagagatca
      121 ccccaccaga aaagggtagg aatgagcaag ttgggaattt tagactgtca ctgcacatgg
      181 acctctggga agacgtctgg cgagagctag gcccactggc cctacagacg gatcttgctg
      241 gctcacctgt ccctgtggag gttcccctgg gaaggcaaga tgcccaacaa cagcactgct
      301 ctgtcattgg ccaatgttac ctacatcacc atggaaattt tcattggact ctgcgccata
      361 gtgggcaacg tgctggtcat ctgcgtggtc aagctgaacc ccagcctgca gaccaccacc
      421 ttctatttca ttgtctctct agccctggct gacattgctg ttggggtgct ggtcatgcct
      481 ttggccattg ttgtcagcct gggcatcaca atccacttct acagctgcct ttttatgact
      541 tgcctactgc ttatctttac ccacgcctcc atcatgtcct tgctggccat cgctgtggac
      601 cgatacttgc gggtcaagct taccgtcaga ttcagaattc ctgggctccc tgggtgcatt
      661 ctatcattcc agttgaaagt ttgcttcctt ccagtcatgt ggctcttcat tctactctcc
      721 ttggctctca tttcagatgc catggtcatg gatgaaaagg tcaagagaag ctttgtgctg
      781 gacacggctt ctgccatctg caactacaat gcccactaca agaatcaccc caaatactgg
      841 tgccgaggct atttccgtga ctactgcaac atcatcgcct tctcccctaa cagcaccaat
      901 catgtggccc tgagggacac agggaaccag ctcattgtca ctatgtcctg cctgaccaaa
      961 gaggacacgg gctggtactg gtgtggcatc cagcgggact ttgccaggga tgacatggat
     1021 tttacagagc tgattgtaac tgacgacaaa ggaaccctgg ccaatgactt ttggtctggg
     1081 aaagacctat caggcaacaa aaccagaagc tgcaaggctc ccaaagttgt ccgcaaggct
     1141 gaccgctcca ggacgtccat tctcatcatt tgcatactga tcacgggttt gggaatcatc
     1201 tctgtaatca gtcatttgac caaaaggagg agaagtcaaa ggaatagaag ggtaggcaac
     1261 actttgaagc ccttctcgcg tgtcctgact ccaaaggaaa tggctcctac tgaacagatg
     1321 tgactgaaga tttttttaat ttagttcata aagtgatgct acaacagaat aatcaccatg
     1381 acaactggcc cacacctcag agactgattc tgatctccca ggaattctga aggaccctct
     1441 atccttgaca acaatcattt gcagccaggt agcaacggcg gtagtcagag gagctatgat
     1501 agaccacacc caagcaaggc tgccctcaaa taacatctca agatcttagt tcttatgcat
     1561 tccatcagtc agaagtgaag aagaggtgga gaatctggat tggggaccag gaaatcactt
     1621 gtattttgtt agccaataaa ttcctagcca gtgttgaatg aa
//
