LOCUS       NM_001320436            1167 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens transmembrane protein 95 (TMEM95), transcript variant
            3, mRNA.
ACCESSION   NM_001320436 XM_005256620
VERSION     NM_001320436.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Tang,S., Lu,Y., Skinner,W.M., Sanyal,M., Lishko,P.V., Ikawa,M. and
            Kim,P.S.
  TITLE     Human sperm TMEM95 binds eggs and facilitates membrane fusion
  JOURNAL   Proc Natl Acad Sci U S A 119 (40), e2207805119 (2022)
   PUBMED   36161911
  REMARK    GeneRIF: Human sperm TMEM95 binds eggs and facilitates membrane
            fusion.
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1167)
  AUTHORS   Lamas-Toranzo,I., Hamze,J.G., Bianchi,E., Fernandez-Fuertes,B.,
            Perez-Cerezales,S., Laguna-Barraza,R., Fernandez-Gonzalez,R.,
            Lonergan,P., Gutierrez-Adan,A., Wright,G.J., Jimenez-Movilla,M. and
            Bermejo-Alvarez,P.
  TITLE     TMEM95 is a sperm membrane protein essential for mammalian
            fertilization
  JOURNAL   Elife 9, e53913 (2020)
   PUBMED   32484434
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1167)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1167)
  AUTHORS   Pausch,H., Kolle,S., Wurmser,C., Schwarzenbacher,H., Emmerling,R.,
            Jansen,S., Trottmann,M., Fuerst,C., Gotz,K.U. and Fries,R.
  TITLE     A nonsense mutation in TMEM95 encoding a nondescript transmembrane
            protein causes idiopathic male subfertility in cattle
  JOURNAL   PLoS Genet 10 (1), e1004044 (2014)
   PUBMED   24391514
REFERENCE   6  (bases 1 to 1167)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC026954.14 and BC107110.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001320436.1.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1. This
            results in a shorter protein (isoform 3), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC107110.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000576060.6/ ENSP00000460828.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC026954.14        76642-76663
            23-644              BC107110.2         1-622
            645-1167            AC026954.14        78183-78705
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..1167
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /note="transmembrane protein 95"
                     /db_xref="GeneID:339168"
                     /db_xref="HGNC:HGNC:27898"
                     /db_xref="MIM:617814"
     exon            1..218
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..580
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 3; sperm-egg fusion protein TMEM95"
                     /codon_start=1
                     /product="sperm-egg fusion protein TMEM95 isoform 3
                     precursor"
                     /protein_id="NP_001307365.1"
                     /db_xref="CCDS:CCDS82051.1"
                     /db_xref="GeneID:339168"
                     /db_xref="HGNC:HGNC:27898"
                     /db_xref="MIM:617814"
                     /translation="MWRLALGGVFLAAAQACVFCRLPAHDLSGRLARLCSQMEARQKE
                     CGASPDFSAFALDEVSMNKVTEKTHRVLRVMEIKEAVSSLPSYWSWLRKTKLPEYTRE
                     ALCPPACRGSTTLYNCSTCKGTEVSCWPRKRCFPGSQDLWEAKILLLSIFGAFLLLGV
                     LSLLVESHHLQAKSGL"
     exon            219..275
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     exon            276..356
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     exon            357..377
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     exon            378..458
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     exon            459..546
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     exon            547..1167
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1146..1151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /note="hexamer: AGTAAA"
     polyA_site      1167
                     /gene="TMEM95"
                     /gene_synonym="UNQ9390"
                     /note="major polyA site"
ORIGIN      
        1 attcctcggc tcagctgggg cagcgccgcc ccatccccca gtggtcctca tgtggaggct
       61 ggcactaggc ggggttttcc tggcagccgc ccaggcttgt gtcttctgtc gcctcccagc
      121 ccacgacttg tcaggccgcc tggctcggct ctgcagccag atggaggcca ggcagaagga
      181 atgtggggcc tccccagact tctcggcctt tgccttagat gaggtgtcca tgaacaaagt
      241 cacagagaag actcacagag tcctgagggt catggaaatc aaagaggctg tctcctcact
      301 cccttcatat tggagttggc ttcgaaagac caagctccct gagtacacca gggaagctct
      361 ctgtcccccc gcctgccggg gcagcaccac gctgtacaac tgctccacct gcaaggggac
      421 ggaggtgtcc tgctggcccc gaaagcgctg cttcccagga agtcaggatc tttgggaagc
      481 caagattctg ctcctctcca tcttcggagc tttcctgctt ctgggtgttc tgagcctcct
      541 ggtggagtcc caccacctcc aagcaaaaag tggcttgtga agacgctgaa aacctcccag
      601 cctccagctc taaggggtat gcactcacaa cttccacatc ccttggaggg gaaccagtca
      661 gccccttagt cccagctcca aagacagtct ccagacccta aaacccagac atccctgctt
      721 ctggttggtg agataatgaa aaacaagaaa atccccaaaa acccagatcc cccacaatcc
      781 cagtgtcaga tggcctcccg ggaacccagg cacccacagc tggaaagttc ctcccctcca
      841 gccctcaacc aatcacatgg ctgtcaacaa tgccaggaaa atatctacag aaggaaagaa
      901 tcccctacgc cactcccacc acacccacac ccccttctgc ctgttccggg aaagcggggg
      961 catctgcccc agaagctatt ccaggccctc ctatgactga tggggaatcc gggaatgcat
     1021 gttctggaaa actcacccca ctagagtgag atcacatcag tgggttcgcg ggcatgccct
     1081 ccctccatcg tgttaacagt ttgaaatcct ggcctccctc agaggcctcc atcctgccag
     1141 gcctaagtaa aacttgctgt tcatggc
//
