LOCUS       NM_001286661            1994 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens transmembrane protein 68 (TMEM68), transcript variant
            4, mRNA.
ACCESSION   NM_001286661
VERSION     NM_001286661.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   McLelland,G.L., Lopez-Osias,M., Verzijl,C.R.C., Ellenbroek,B.D.,
            Oliveira,R.A., Boon,N.J., Dekker,M., van den Hengel,L.G., Ali,R.,
            Janssen,H., Song,J.Y., Krimpenfort,P., van Zutphen,T., Jonker,J.W.
            and Brummelkamp,T.R.
  TITLE     Identification of an alternative triglyceride biosynthesis pathway
  JOURNAL   Nature 621 (7977), 171-178 (2023)
   PUBMED   37648867
  REMARK    GeneRIF: Identification of an alternative triglyceride biosynthesis
            pathway.
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Wang,Y., Zeng,F., Zhao,Z., He,L., He,X., Pang,H., Huang,F. and
            Chang,P.
  TITLE     Transmembrane Protein 68 Functions as an MGAT and DGAT Enzyme for
            Triacylglycerol Biosynthesis
  JOURNAL   Int J Mol Sci 24 (3), 2012 (2023)
   PUBMED   36768334
  REMARK    GeneRIF: Transmembrane Protein 68 Functions as an MGAT and DGAT
            Enzyme for Triacylglycerol Biosynthesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1994)
  AUTHORS   Chang,P., Heier,C., Qin,W., Han,L., Huang,F. and Sun,Q.
  TITLE     Molecular identification of transmembrane protein 68 as an
            endoplasmic reticulum-anchored and brain-specific protein
  JOURNAL   PLoS One 12 (5), e0176980 (2017)
   PUBMED   28472192
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1994)
  AUTHORS   Zhan,M., Chen,G., Pan,C.M., Gu,Z.H., Zhao,S.X., Liu,W., Wang,H.N.,
            Ye,X.P., Xie,H.J., Yu,S.S., Liang,J., Gao,G.Q., Yuan,G.Y.,
            Zhang,X.M., Zuo,C.L., Su,B., Huang,W., Ning,G., Chen,S.J.,
            Chen,J.L. and Song,H.D.
  CONSRTM   China Consortium for Genetics of Autoimmune Thyroid Disease
  TITLE     Genome-wide association study identifies a novel susceptibility
            gene for serum TSH levels in Chinese populations
  JOURNAL   Hum Mol Genet 23 (20), 5505-5517 (2014)
   PUBMED   24852370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC100817.5, CN272366.1 and DB370750.1.
            
            On May 31, 2019 this sequence version replaced NM_001286661.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR, lacks a
            portion of the 5' coding region and initiates translation from a
            downstream AUG compared to variant 1. This variant also lacks an
            exon in the 3' coding region which results in a frameshift compared
            to variant 1. The encoded isoform (4) has a shorter N-terminus and
            a distinct C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CN272366.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC100817.5         150191-150234       c
            45-556              CN272366.1         1-512
            557-1991            AC100817.5         115617-117051       c
            1992-1994           DB370750.1         15-17               c
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.1"
     gene            1..1994
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="transmembrane protein 68"
                     /db_xref="GeneID:137695"
                     /db_xref="HGNC:HGNC:26510"
     exon            1..110
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            111..278
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..562
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.22"
                     /note="isoform 4 is encoded by transcript variant 4;
                     2-acylglycerol/1,2-diacylglycerol O-acyltransferase;
                     monoacylglycerol/Diacylglycerol O-acyltransferase;
                     DGAT1/2-independent enzyme synthesizing storage lipids"
                     /codon_start=1
                     /product="DGAT1/2-independent enzyme synthesizing storage
                     lipids isoform 4"
                     /protein_id="NP_001273590.1"
                     /db_xref="CCDS:CCDS75740.1"
                     /db_xref="GeneID:137695"
                     /db_xref="HGNC:HGNC:26510"
                     /translation="MEKIPEDGPALIIFYHGAIPIDFYYFMAKIFIHKGRTCRVVADH
                     FVFKIPGFSLLLDVFCALHGPREKCVEILRSGHLLAISPGGVREALISDETYNIVWGH
                     RRGFAQVAIDAKVPIIPMFTQNIREGFRSLGGTNEECCSSFD"
     exon            279..472
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            473..533
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            534..1994
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1974..1979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="hexamer: ATTAAA"
     polyA_site      1994
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="major polyA site"
ORIGIN      
        1 agccgccgct tggcttcttt cacacgggtt gcttcggagg aatccgccgt gcaaatctgt
       61 ccgccccctt ggccactgat cccccgaaga gcttctgtcg ccgctctagg gttatgaagt
      121 tcatggaatg gaaaaaatac cagaagatgg accagcactt ataatttttt atcatggagc
      181 tattcctata gatttttact atttcatggc taaaatattt atacacaaag gcagaacttg
      241 ccgagtagta gctgatcact ttgtctttaa aattccaggg tttagtttat tactggatgt
      301 gttttgtgct ctacatggac caagagaaaa atgtgttgaa attctgagga gtggccactt
      361 gttagctatc tcaccaggtg gagttcgaga agccctaatt agtgatgaaa cttataacat
      421 cgtatggggt catcgcagag gctttgctca ggttgcaatt gatgcaaaag tgcccattat
      481 tcctatgttt acacaaaata ttcgagaagg atttagatca cttggaggaa caaacgaaga
      541 atgctgttca agctttgatt gataagcacc aaagaatacc aggaaacatt atgagtgctt
      601 tgttagaacg ttttcattga taacaaaggg tcaactagaa gatgatttag tacatttata
      661 ttaaatgttt gtatctaagg tactgtcttc tgaattttgt aggtcctata attagtattt
      721 tttaaaaaaa tcatgttaat aagcatcttt cacagaattc gtttctttaa aatagtcaat
      781 tttgtttttg caattgtgtc aaatactaac aaattacaca cctagtaatt cagaaaaaga
      841 tgtcttattt gtaaattcct aacaatttat gctaaacata tagattctta agtttattaa
      901 taacagcagt ttaggttaaa caaacattcc tggataatgc gttaaatttc tgtatctgtc
      961 gccctgagct gattttgaaa gatggtataa gctaggggtt agtatagttg tttaagttag
     1021 aaaaaacatg ctgttgtctg cccctcattc ccttcatgac cttgggcaag tcacgtaatg
     1081 tttttgtgcc tcaacaattc actttttaaa aacatgatcg tatgatgaat gatattattt
     1141 tgttatttat atttactgtg attgataact gttgaaccaa aataataaaa taattaattt
     1201 aaacaatgtc aaaatccttt agcagttatg tatatatttt ctccattgtg tgtttaaatt
     1261 atgtcatgtc cagttgccaa gcacaatgaa aaagatgtat tattttttaa attgaataaa
     1321 aaattaggaa aaataaaatt tctaattatt atttttagta tgatattttt aacaagagtc
     1381 tataggcaaa caatataggg tgtgctgtgc attgtcagcc ctatactgtg gtcttaataa
     1441 tgccagctta aaaatcactg ttgtgctctg catttcgtgt gttagaagct gattctaggc
     1501 tgaggaaagc aagagttctc tacttttgct caatattgag gcttacccag tttgactcta
     1561 cagccagtga agtggtttat tgcttcaata aaaatatact tgaatgatga atttatttat
     1621 gttttgtttt gtttttattt agagatgggg ttttgccatg ttggccaggc tggtcttgaa
     1681 ctcctgacct caggtgatcc acctgcctca gcctcccaac ttgctgggat tacaggtgtg
     1741 agccaccatg cccagcctga atgatgaatt tatgtgaaca caaagccctc tgaaaaatta
     1801 atgattttca atttgttgtc ttcttgaaaa ctgaatttaa gtattattta aacagaaaag
     1861 tgtcagaaat caaatgcagt atctccctct ccctttttta aatgttgaat tagtagggtt
     1921 tttgtatagc tttttgtatt gtgtgtattt atgatatgtg aaatgtacat aaaattaaac
     1981 aatgtgaaaa taaa
//
