LOCUS       NM_001286657            2573 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transmembrane protein 68 (TMEM68), transcript variant
            1, mRNA.
ACCESSION   NM_001286657 XM_005251150
VERSION     NM_001286657.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   McLelland,G.L., Lopez-Osias,M., Verzijl,C.R.C., Ellenbroek,B.D.,
            Oliveira,R.A., Boon,N.J., Dekker,M., van den Hengel,L.G., Ali,R.,
            Janssen,H., Song,J.Y., Krimpenfort,P., van Zutphen,T., Jonker,J.W.
            and Brummelkamp,T.R.
  TITLE     Identification of an alternative triglyceride biosynthesis pathway
  JOURNAL   Nature 621 (7977), 171-178 (2023)
   PUBMED   37648867
  REMARK    GeneRIF: Identification of an alternative triglyceride biosynthesis
            pathway.
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wang,Y., Zeng,F., Zhao,Z., He,L., He,X., Pang,H., Huang,F. and
            Chang,P.
  TITLE     Transmembrane Protein 68 Functions as an MGAT and DGAT Enzyme for
            Triacylglycerol Biosynthesis
  JOURNAL   Int J Mol Sci 24 (3), 2012 (2023)
   PUBMED   36768334
  REMARK    GeneRIF: Transmembrane Protein 68 Functions as an MGAT and DGAT
            Enzyme for Triacylglycerol Biosynthesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2573)
  AUTHORS   Chang,P., Heier,C., Qin,W., Han,L., Huang,F. and Sun,Q.
  TITLE     Molecular identification of transmembrane protein 68 as an
            endoplasmic reticulum-anchored and brain-specific protein
  JOURNAL   PLoS One 12 (5), e0176980 (2017)
   PUBMED   28472192
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2573)
  AUTHORS   Zhan,M., Chen,G., Pan,C.M., Gu,Z.H., Zhao,S.X., Liu,W., Wang,H.N.,
            Ye,X.P., Xie,H.J., Yu,S.S., Liang,J., Gao,G.Q., Yuan,G.Y.,
            Zhang,X.M., Zuo,C.L., Su,B., Huang,W., Ning,G., Chen,S.J.,
            Chen,J.L. and Song,H.D.
  CONSRTM   China Consortium for Genetics of Autoimmune Thyroid Disease
  TITLE     Genome-wide association study identifies a novel susceptibility
            gene for serum TSH levels in Chinese populations
  JOURNAL   Hum Mol Genet 23 (20), 5505-5517 (2014)
   PUBMED   24852370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA121422.1, AK056932.1, AL832935.1, AC100817.5 and DB370750.1.
            
            On or before May 10, 2018 this sequence version replaced
            XM_005251150.1, NM_001286657.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.800677.1,
                                           SRR1803615.39285.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000434581.7/ ENSP00000395204.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               DA121422.1         1-110
            111-501             AK056932.1         59-449
            502-1839            AL832935.1         1-1338
            1840-2570           AC100817.5         115617-116347       c
            2571-2573           DB370750.1         15-17               c
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.1"
     gene            1..2573
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="transmembrane protein 68"
                     /db_xref="GeneID:137695"
                     /db_xref="HGNC:HGNC:26510"
     exon            1..110
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            111..155
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            156..549
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             225..1199
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     2-acylglycerol/1,2-diacylglycerol O-acyltransferase;
                     monoacylglycerol/Diacylglycerol O-acyltransferase;
                     DGAT1/2-independent enzyme synthesizing storage lipids"
                     /codon_start=1
                     /product="DGAT1/2-independent enzyme synthesizing storage
                     lipids isoform 1"
                     /protein_id="NP_001273586.1"
                     /db_xref="CCDS:CCDS75742.1"
                     /db_xref="GeneID:137695"
                     /db_xref="HGNC:HGNC:26510"
                     /translation="MIDKNQTCGVGQDSVPYMICLIHILEEWFGVEQLEDYLNFANYL
                     LWVFTPLILLILPYFTIFLLYLTIIFLHIYKRKNVLKEAYSHNLWDGARKTVATLWDG
                     HAAVWHGYEVHGMEKIPEDGPALIIFYHGAIPIDFYYFMAKIFIHKGRTCRVVADHFV
                     FKIPGFSLLLDVFCALHGPREKCVEILRSGHLLAISPGGVREALISDETYNIVWGHRR
                     GFAQVAIDAKVPIIPMFTQNIREGFRSLGGTRLFRWLYEKFRYPFAPMYGGFPVKLRT
                     YLGDPIPYDPQITAEELAEKTKNAVQALIDKHQRIPGNIMSALLERFH"
     exon            550..717
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            718..911
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            912..972
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1112
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..2573
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2553..2558
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="hexamer: ATTAAA"
     polyA_site      2573
                     /gene="TMEM68"
                     /gene_synonym="DIESL; MGAT/DGAT"
                     /note="major polyA site"
ORIGIN      
        1 agccgccgct tggcttcttt cacacgggtt gcttcggagg aatccgccgt gcaaatctgt
       61 ccgccccctt ggccactgat cccccgaaga gcttctgtcg ccgctctagg aatagagaca
      121 ttgaagtttg ggacaagata tttatctaac ttctgtgtca aaattagcga cctgctatgg
      181 caatgaagaa agaaactgaa tttgtcattt tcacctgaag aaaaatgata gacaaaaatc
      241 aaacctgtgg tgtaggacag gattctgtgc cctatatgat ttgtctgatt cacatactcg
      301 aagaatggtt tggtgtggag cagttggagg actatttgaa ttttgcaaac tatctcttgt
      361 gggtttttac accactaata cttttaatac ttccttactt tactatcttt cttctctacc
      421 ttactattat tttcttacac atttataaga gaaagaatgt attgaaagaa gcctactctc
      481 ataatttatg ggatggtgca aggaaaacag tggcaactct gtgggatgga catgcagccg
      541 tttggcatgg ttatgaagtt catggaatgg aaaaaatacc agaagatgga ccagcactta
      601 taatttttta tcatggagct attcctatag atttttacta tttcatggct aaaatattta
      661 tacacaaagg cagaacttgc cgagtagtag ctgatcactt tgtctttaaa attccagggt
      721 ttagtttatt actggatgtg ttttgtgctc tacatggacc aagagaaaaa tgtgttgaaa
      781 ttctgaggag tggccacttg ttagctatct caccaggtgg agttcgagaa gccctaatta
      841 gtgatgaaac ttataacatc gtatggggtc atcgcagagg ctttgctcag gttgcaattg
      901 atgcaaaagt gcccattatt cctatgttta cacaaaatat tcgagaagga tttagatcac
      961 ttggaggaac aaggttattt aggtggcttt atgaaaaatt ccgctatcca tttgctccaa
     1021 tgtatggagg ttttccagtg aagttacgga cctatttagg cgaccccatt ccgtatgacc
     1081 cacagataac agcggaagaa ttagctgaaa agacgaagaa tgctgttcaa gctttgattg
     1141 ataagcacca aagaatacca ggaaacatta tgagtgcttt gttagaacgt tttcattgat
     1201 aacaaagggt caactagaag atgatttagt acatttatat taaatgtttg tatctaaggt
     1261 actgtcttct gaattttgta ggtcctataa ttagtatttt ttaaaaaaat catgttaata
     1321 agcatctttc acagaattcg tttctttaaa atagtcaatt ttgtttttgc aattgtgtca
     1381 aatactaaca aattacacac ctagtaattc agaaaaagat gtcttatttg taaattccta
     1441 acaatttatg ctaaacatat agattcttaa gtttattaat aacagcagtt taggttaaac
     1501 aaacattcct ggataatgcg ttaaatttct gtatctgtcg ccctgagctg attttgaaag
     1561 atggtataag ctaggggtta gtatagttgt ttaagttaga aaaaacatgc tgttgtctgc
     1621 ccctcattcc cttcatgacc ttgggcaagt cacgtaatgt ttttgtgcct caacaattca
     1681 ctttttaaaa acatgatcgt atgatgaatg atattatttt gttatttata tttactgtga
     1741 ttgataactg ttgaaccaaa ataataaaat aattaattta aacaatgtca aaatccttta
     1801 gcagttatgt atatattttc tccattgtgt gtttaaatta tgtcatgtcc agttgccaag
     1861 cacaatgaaa aagatgtatt attttttaaa ttgaataaaa aattaggaaa aataaaattt
     1921 ctaattatta tttttagtat gatattttta acaagagtct ataggcaaac aatatagggt
     1981 gtgctgtgca ttgtcagccc tatactgtgg tcttaataat gccagcttaa aaatcactgt
     2041 tgtgctctgc atttcgtgtg ttagaagctg attctaggct gaggaaagca agagttctct
     2101 acttttgctc aatattgagg cttacccagt ttgactctac agccagtgaa gtggtttatt
     2161 gcttcaataa aaatatactt gaatgatgaa tttatttatg ttttgttttg tttttattta
     2221 gagatggggt tttgccatgt tggccaggct ggtcttgaac tcctgacctc aggtgatcca
     2281 cctgcctcag cctcccaact tgctgggatt acaggtgtga gccaccatgc ccagcctgaa
     2341 tgatgaattt atgtgaacac aaagccctct gaaaaattaa tgattttcaa tttgttgtct
     2401 tcttgaaaac tgaatttaag tattatttaa acagaaaagt gtcagaaatc aaatgcagta
     2461 tctccctctc ccttttttaa atgttgaatt agtagggttt ttgtatagct ttttgtattg
     2521 tgtgtattta tgatatgtga aatgtacata aaattaaaca atgtgaaaat aaa
//
