LOCUS       NM_153704               4678 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens transmembrane protein 67 (TMEM67), transcript variant
            1, mRNA.
ACCESSION   NM_153704
VERSION     NM_153704.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Pang,J., Kong,F., Tang,W., Xi,H., Ma,N., Sheng,X., Peng,Y. and
            Liu,Z.
  TITLE     Two novel TMEM67 variations in a Chinese family with recurrent
            pregnancy loss: a case report
  JOURNAL   BMC Med Genomics 17 (1), 156 (2024)
   PUBMED   38844949
  REMARK    GeneRIF: Two novel TMEM67 variations in a Chinese family with
            recurrent pregnancy loss: a case report.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Yinsheng,Z., Miyoshi,K., Qin,Y., Fujiwara,Y., Yoshimura,T. and
            Katayama,T.
  TITLE     TMEM67 is required for the gating function of the transition zone
            that controls entry of membrane-associated proteins ARL13B and
            INPP5E into primary cilia
  JOURNAL   Biochem Biophys Res Commun 636 (Pt 1), 162-169 (2022)
   PUBMED   36334440
  REMARK    GeneRIF: TMEM67 is required for the gating function of the
            transition zone that controls entry of membrane-associated proteins
            ARL13B and INPP5E into primary cilia.
REFERENCE   3  (bases 1 to 4678)
  AUTHORS   Qiu,Y.L., Wang,L., Huang,M., Lian,M., Wang,F., Gong,Y., Ma,X.,
            Hao,C.Z., Zhang,J., Li,Z.D., Xing,Q.H., Cao,M. and Wang,J.S.
  TITLE     Association of novel TMEM67 variants with mild phenotypes of high
            gamma-glutamyl transpeptidase cholestasis and congenital hepatic
            fibrosis
  JOURNAL   J Cell Physiol 237 (6), 2713-2723 (2022)
   PUBMED   35621037
  REMARK    GeneRIF: Association of novel TMEM67 variants with mild phenotypes
            of high gamma-glutamyl transpeptidase cholestasis and congenital
            hepatic fibrosis.
REFERENCE   4  (bases 1 to 4678)
  AUTHORS   Baala,L., Romano,S., Khaddour,R., Saunier,S., Smith,U.M.,
            Audollent,S., Ozilou,C., Faivre,L., Laurent,N., Foliguet,B.,
            Munnich,A., Lyonnet,S., Salomon,R., Encha-Razavi,F., Gubler,M.C.,
            Boddaert,N., de Lonlay,P., Johnson,C.A., Vekemans,M., Antignac,C.
            and Attie-Bitach,T.
  TITLE     The Meckel-Gruber syndrome gene, MKS3, is mutated in Joubert
            syndrome
  JOURNAL   Am J Hum Genet 80 (1), 186-194 (2007)
   PUBMED   17160906
  REMARK    GeneRIF: identified MKS3 mutations in four patients with JS, thus
            defining MKS3 as the sixth JS locus (JBTS6)
REFERENCE   5  (bases 1 to 4678)
  AUTHORS   Smith,U.M., Consugar,M., Tee,L.J., McKee,B.M., Maina,E.N.,
            Whelan,S., Morgan,N.V., Goranson,E., Gissen,P., Lilliquist,S.,
            Aligianis,I.A., Ward,C.J., Pasha,S., Punyashthiti,R., Malik
            Sharif,S., Batman,P.A., Bennett,C.P., Woods,C.G., McKeown,C.,
            Bucourt,M., Miller,C.A., Cox,P., Algazali,L., Trembath,R.C.,
            Torres,V.E., Attie-Bitach,T., Kelly,D.A., Maher,E.R., Gattone,V.H.
            2nd, Harris,P.C. and Johnson,C.A.
  TITLE     The transmembrane protein meckelin (MKS3) is mutated in
            Meckel-Gruber syndrome and the wpk rat
  JOURNAL   Nat Genet 38 (2), 191-196 (2006)
   PUBMED   16415887
  REMARK    GeneRIF: Positional cloning of the Wpk gene suggested a MKS3
            candidate gene, TMEM67, for which we identified pathogenic
            mutations for five MKS3-linked consanguineous families.
REFERENCE   6  (bases 1 to 4678)
  AUTHORS   Morgan,N.V., Gissen,P., Sharif,S.M., Baumber,L., Sutherland,J.,
            Kelly,D.A., Aminu,K., Bennett,C.P., Woods,C.G., Mueller,R.F.,
            Trembath,R.C., Maher,E.R. and Johnson,C.A.
  TITLE     A novel locus for Meckel-Gruber syndrome, MKS3, maps to chromosome
            8q24
  JOURNAL   Hum Genet 111 (4-5), 456-461 (2002)
   PUBMED   12384791
  REMARK    GeneRIF: mapping to chromosome 8 and possible role in Meckel-Gruber
            syndrome
REFERENCE   7  (bases 1 to 4678)
  AUTHORS   Stokman,M., Lilien,M. and Knoers,N.
  TITLE     Nephronophthisis-Related Ciliopathies
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27336129
REFERENCE   8  (bases 1 to 4678)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
REFERENCE   9  (bases 1 to 4678)
  AUTHORS   Bardakjian,T., Weiss,A. and Schneider,A.
  TITLE     Microphthalmia/Anophthalmia/Coloboma Spectrum - RETIRED CHAPTER,
            FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301552
REFERENCE   10 (bases 1 to 4678)
  AUTHORS   Parisi,M. and Glass,I.
  TITLE     Joubert Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK308071.1, BC031220.1,
            BC054338.1, AC010834.19 and BM310097.1.
            
            On Aug 31, 2019 this sequence version replaced NM_153704.5.
            
            Summary: The protein encoded by this gene localizes to the primary
            cilium and to the plasma membrane. The gene functions in centriole
            migration to the apical membrane and formation of the primary
            cilium. Multiple transcript variants encoding different isoforms
            have been found for this gene. Defects in this gene are a cause of
            Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6).
            [provided by RefSeq, Nov 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054338.1, BX648768.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000453321.8/ ENSP00000389998.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                AK308071.1         51-62
            13-23               BC031220.1         1-11
            24-1830             BC054338.1         1-1807
            1831-1831           AC010834.19        147283-147283
            1832-3476           BC054338.1         1809-3453
            3477-4456           AC010834.19        168266-169245
            4457-4676           BM310097.1         9-228
            4677-4678           AC010834.19        169466-169467
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.1"
     gene            1..4678
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="transmembrane protein 67"
                     /db_xref="GeneID:91147"
                     /db_xref="HGNC:HGNC:28396"
                     /db_xref="MIM:609884"
     exon            1..244
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..3009
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; meckel syndrome type 3 protein"
                     /codon_start=1
                     /product="meckelin isoform 1 precursor"
                     /protein_id="NP_714915.3"
                     /db_xref="CCDS:CCDS6258.2"
                     /db_xref="GeneID:91147"
                     /db_xref="HGNC:HGNC:28396"
                     /db_xref="MIM:609884"
                     /translation="MATRGGAGVAMAVWSLLSARAVTAFLLLFLPRFLQAQTFSFPFQ
                     QPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPE
                     NMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSF
                     MVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVG
                     MSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFE
                     NTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVA
                     ASYDIRGNFLKWQTLEGGVLQLCPDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTP
                     IFYDVYLEYTDENQHQYILAVPVLNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSG
                     RENDLGTQPRVIRVATQISLSVHLVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVS
                     FSVTYEMDHGEAHVQTDIALGVLGGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVY
                     YAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPIQEERFVTYVGCAFALKALQF
                     LHKLISQITIDVFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQT
                     VRKINSLFQVLTVLFFLEVVGFKNLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALW
                     LAIGIIQVVFFAVFYERFIEDKIRQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHA
                     DTNMEEMNMNLKREAENLCSQRGLVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGP
                     ARLLSSSASTFEQSIKAYHMMNKFLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPM
                     EKSIFYNDEGYSFSSVLYYGNEATLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFR
                     YIRNTVGQKNLASKTLVDQRFLI"
     exon            245..333
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            334..427
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            428..527
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            528..597
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            598..672
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            673..735
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            736..890
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            891..999
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1086
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1152
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1309
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1433
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1539
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1596
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1695
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1794
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1881
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1981
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2121
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2262
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2343
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2460
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2577
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2682
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2785
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2928
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..4678
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3447..3452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="hexamer: AATAAA"
     polyA_site      3476
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="major polyA site"
     regulatory      4202..4207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="hexamer: AATATA"
     polyA_site      4224
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
     regulatory      4584..4589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="hexamer: GATAAA"
     regulatory      4589..4594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="hexamer: ATTAAA"
     polyA_site      4602
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
     polyA_site      4614
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
     regulatory      4653..4658
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
                     /note="hexamer: CATAAA"
     polyA_site      4678
                     /gene="TMEM67"
                     /gene_synonym="JBTS6; MECKELIN; MKS3; NPHP11; TNEM67"
ORIGIN      
        1 gaggcttcca gcgtcggtac catggcgacg cgcggtgggg ctggggtggc aatggcggtt
       61 tggtccctct tatccgcccg ggccgtgacc gcgttccttc tgttgttcct ccctcgcttc
      121 ttacaggccc agaccttctc tttccctttc cagcagccgg agaagtgcga caacaaccag
      181 tactttgata tctccgccct ctcgtgtgtt ccttgtggag ctaaccagag gcaagatgcc
      241 cgaggaactt catgtgtatg tctaccagga tttcagatga tctctaataa tggaggacct
      301 gctattattt gtaaaaagtg cccagaaaac atgaaaggtg ttacagaaga tggctggaac
      361 tgcatttctt gccctagtga cttaactgcc gaaggaaaat gtcactgtcc cattggccat
      421 attttagtgg aaagagacat taatggaaca ttgttgtctc aagcaacttg tgagctctgt
      481 gatggaaatg aaaactcttt tatggtagta aatgctttag gagacaggtg cgtccgatgt
      541 gagccaacat ttgttaatac cagcaggtcc tgtgcatgtt cagaacctaa cattttaaca
      601 gggggattat gtttcagcag cacagggaat tttcctctac gtagaatttc agctgcacgt
      661 tatggagaag ttggcatgtc tttaacttca gaatggtttg caaagtattt gcaatcatca
      721 gcagctgcat gttgggtata tgccaatcta acatcttgtc aagctcttgg aaatatgtgt
      781 gtgatgaaca tgaattctta cgactttgcc acatttgatg catgtggact atttcagttt
      841 atctttgaaa atactgctgg actgagcact gttcattcta tttcattttg gagacagaat
      901 cttccttggc tgttttatgg agaccagtta ggattagcac ctcaagtgct cagttctacc
      961 tctcttccta caaatttcag ttttaaagga gaaaaccaga atacaaaact gaagtttgtt
     1021 gctgcttcct atgatataag aggaaatttt ctcaagtggc aaactttaga aggaggtgtt
     1081 ttacagcttt gtccagacac agagacaagg ctaaatgctg cttattcatt tggaacaacc
     1141 taccaacaaa attgtgagat tcctatctct aagatcttaa ttgactttcc cactcctata
     1201 ttttatgatg tgtaccttga atatactgat gaaaatcaac atcaatatat tttggctgtg
     1261 cctgtgttaa acctaaatct tcaacataat aagatatttg tgaaccaaga cagcaactct
     1321 ggaaagtggc ttctaactcg gcgcattttc ttagtggatg cagtaagtgg acgagaaaat
     1381 gacttaggaa ctcagccaag agtaattcga gttgctactc aaatatcact gagtgtccac
     1441 cttgtaccca acacaataaa tggaaacatc taccctccct taatcaccat tgcctacagt
     1501 gacattgata tcaaagatgc caacagccag tctgtgaagg tttctttctc agtcacatat
     1561 gaaatggatc atggagaagc acatgtccag acagatattg ctttgggtgt attgggtggg
     1621 ctagctgttt tagcatctct tttgaagaca gcaggatgga agaggcgcat tgggagtccc
     1681 atgattgatt tacagacagt tgtgaaattc ttggtgtact atgctggtga tctggccaat
     1741 gttttcttta tcatcacagt gggaacaggt ctttactggc ttattttctt caaagcacag
     1801 aagtctgtgt ctgttttgct gccaatgcca attcaggaag aacgttttgt cacttatgtt
     1861 ggatgtgcct ttgctctgaa ggcactacaa tttttgcata agctcatatc ccagattaca
     1921 atagatgtat tctttattga ttgggagcga cctaaaggaa aggttcttaa agctgttgaa
     1981 ggtgagggtg gtgtacgaag tgccactgtt cctgtaagca tatggagaac atattttgta
     2041 gcaaatgaat ggaatgaaat tcagactgtg agaaaaatta attcactctt tcaagtactt
     2101 actgtcctct tctttttgga ggttgtggga ttcaagaact tagcattaat ggactcatct
     2161 tctagtcttt ctagaaaccc acctagctac atagctcctt atagctgcat tttgagatat
     2221 gcagtgtctg ctgctctttg gctagccatt ggaattatac aggtcgtgtt ctttgctgtc
     2281 ttttatgaga gatttataga agataaaatt cgacagttcg ttgatttatg ctctatgagt
     2341 aatatatcag tgtttctgtt atcccacaaa tgttttggat attacattca tggtagatca
     2401 gtacatgggc atgcagatac taatatggaa gaaatgaata tgaaccttaa aagagaagcg
     2461 gaaaatttgt gtagccagag aggtttggta cccaacacag atggtcagac ttttgagatt
     2521 gcaatttcta accagatgag acaacattat gacagaattc atgagacact aataaggaaa
     2581 aatggtcctg ctagactact gagttcatca gcaagtactt ttgagcagag tataaaagca
     2641 tatcatatga tgaataaatt tcttggctcc ttcattgacc atgttcataa ggaaatggat
     2701 tactttataa aagataagtt gcttcttgaa agaattcttg gaatggaatt catggaacca
     2761 atggaaaaaa gcatctttta caatgatgaa ggttattctt tcagcagtgt cctgtattat
     2821 ggaaatgaag ctactcttct tatttttgat ctgctgttct tctgtgttgt ggatttggct
     2881 tgccaaaatt ttattttagc atccttcctt acatatctac aacaagagat ttttagatat
     2941 atccgtaata cagtaggaca aaagaatttg gcatccaaaa cattggtgga tcaaagattt
     3001 ttgatttaac ttcctgaata aataacttaa agactcagta taatcatggc caaaaaaaag
     3061 tcatgatatc aggttagttt gccatatttt ttaaaactta taatgcagac tattctgttt
     3121 ttgtttttta tttgcagaaa gatttatttt tataacttgg gaatatataa cctgatataa
     3181 tagaaaatac tgttttccca ttgtgtgtag tatttaatgc agtaaataat aaattaatac
     3241 tgacagtgag gccaaaaaaa atctaaatgt tgctttcagc ataatacttt ttaatgctaa
     3301 ttaaaacagt ccccatcatt tgtctctgta gattatttta taaactgaga gctgcctgaa
     3361 tcttctcaca ttgacaaagt aatttgattc caagtttaag actagttttc agtttgatat
     3421 tttcatgttt tgtgtgctta tggttaaata aatgttgaaa gatattttca agtttactta
     3481 gaaattttaa attgtattat tgctgattca atttgcatca ttctaattgc tcatcctctt
     3541 tggcagtgga ttaattcact gttgggttaa tatgcatctc atgaaatgat atcttttcat
     3601 tcctttgtat acctgtatta ggtcagtata cgctttccat gaaatatatt tgcatttcta
     3661 atatagttta catttattaa gttataacgt atgacatttt tcccaatatc attttttata
     3721 cattttaatt acttaatgtt ttaagagttc tcttccagag ttttgctttt caattacgtt
     3781 taaagactgt aaataacaaa cagtaaggct gggcgctgtg gcttacgcct gtaatcccag
     3841 cactttggaa ggccaaggca cgtggatcac tttaggtcag gagttcaaga acagcctggc
     3901 caacatggaa aaccccatct ctacaaaaaa tacaaaaatt agttggatgt ggtggcctgt
     3961 aatcccagct atttgggagg ctgaggcatg agaatagctt gaaaccagga ggtggaggct
     4021 gtagtgagcc agatagcacc actgcactcc agccaggatg acagagcaag accctgtctc
     4081 agaaaaaaca aaaacaaaac actgtaaatg tatcatgttt ctcactgctt tctatgttaa
     4141 tgctgtgtaa atacttaaaa tggaatactg tataaatatt atttatattt tctcagatga
     4201 aaatatatac taaaacattt ggtatttctg ttttctgttt cattaatgta tatctgatgg
     4261 agtaccattt aggaatataa cattattggg gcagaggtcc cagccattgc caaagttgaa
     4321 tcttttacat ttccctgaat cttgaggatg actgaaaagg aagtctttga aactgcagtt
     4381 tctgaaggat cacagtacag gaatgccttt ataaaagctg cagagataaa tacatgtact
     4441 tatatggcat ggatatttaa aaccactcac tcagaagcat atgaaagaaa ttcaaccatc
     4501 attcaacata gaagaagtta attttagtgc aaaagttttt aagtattaaa tttcacaata
     4561 aaggatcaat tggcttactt gctgataaat taaaacagaa aacatttaaa atcattctaa
     4621 attttgtatg atatttttat tttggctagg tgcataaaaa tgtgttcaga ttttcata
//
