LOCUS       NM_015012               3798 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transmembrane protein 41B (TMEM41B), transcript
            variant 1, mRNA.
ACCESSION   NM_015012 XM_495866
VERSION     NM_015012.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Hu,H., Chen,Z., Huang,S., Fu,C. and Chen,L.
  TITLE     TMEM41B, a novel ER phospholipid scramblase mediating systemic
            lipid metabolism
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 54 (11), 1761-1764 (2022)
   PUBMED   36604140
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Ji,M., Li,M., Sun,L., Zhao,H., Li,Y., Zhou,L., Yang,Z., Zhao,X.,
            Qu,W., Xue,H., Zheng,Z., Li,Y., Deng,H. and Zhao,Y.G.
  TITLE     VMP1 and TMEM41B are essential for DMV formation during
            beta-coronavirus infection
  JOURNAL   J Cell Biol 221 (6) (2022)
   PUBMED   35536318
  REMARK    GeneRIF: VMP1 and TMEM41B are essential for DMV formation during
            beta-coronavirus infection.
REFERENCE   3  (bases 1 to 3798)
  AUTHORS   Huang,D., Xu,B., Liu,L., Wu,L., Zhu,Y., Ghanbarpour,A., Wang,Y.,
            Chen,F.J., Lyu,J., Hu,Y., Kang,Y., Zhou,W., Wang,X., Ding,W.,
            Li,X., Jiang,Z., Chen,J., Zhang,X., Zhou,H., Li,J.Z., Guo,C.,
            Zheng,W., Zhang,X., Li,P., Melia,T., Reinisch,K. and Chen,X.W.
  TITLE     TMEM41B acts as an ER scramblase required for lipoprotein
            biogenesis and lipid homeostasis
  JOURNAL   Cell Metab 33 (8), 1655-1670 (2021)
   PUBMED   34015269
REFERENCE   4  (bases 1 to 3798)
  AUTHORS   Li,Y.E., Wang,Y., Du,X., Zhang,T., Mak,H.Y., Hancock,S.E.,
            McEwen,H., Pandzic,E., Whan,R.M., Aw,Y.C., Lukmantara,I.E.,
            Yuan,Y., Dong,X., Don,A., Turner,N., Qi,S. and Yang,H.
  TITLE     TMEM41B and VMP1 are scramblases and regulate the distribution of
            cholesterol and phosphatidylserine
  JOURNAL   J Cell Biol 220 (6) (2021)
   PUBMED   33929485
REFERENCE   5  (bases 1 to 3798)
  AUTHORS   Trimarco,J.D., Heaton,B.E., Chaparian,R.R., Burke,K.N.,
            Binder,R.A., Gray,G.C., Smith,C.M., Menachery,V.D. and Heaton,N.S.
  TITLE     TMEM41B is a host factor required for the replication of diverse
            coronaviruses including SARS-CoV-2
  JOURNAL   PLoS Pathog 17 (5), e1009599 (2021)
   PUBMED   34043740
  REMARK    GeneRIF: TMEM41B is a host factor required for the replication of
            diverse coronaviruses including SARS-CoV-2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3798)
  AUTHORS   Shoemaker,C.J., Huang,T.Q., Weir,N.R., Polyakov,N.J., Schultz,S.W.
            and Denic,V.
  TITLE     CRISPR screening using an expanded toolkit of autophagy reporters
            identifies TMEM41B as a novel autophagy factor
  JOURNAL   PLoS Biol 17 (4), e2007044 (2019)
   PUBMED   30933966
  REMARK    GeneRIF: TMEM41B is an integral endoplasmic reticulum (ER) membrane
            protein distantly related to the established autophagy factor
            vacuole membrane protein 1 (VMP1), and the data show that these two
            factors play related, albeit not fully overlapping, roles in
            autophagosome biogenesis.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3798)
  AUTHORS   Van Alstyne,M., Lotti,F., Dal Mas,A., Area-Gomez,E. and
            Pellizzoni,L.
  TITLE     Stasimon/Tmem41b localizes to mitochondria-associated ER membranes
            and is essential for mouse embryonic development
  JOURNAL   Biochem Biophys Res Commun 506 (3), 463-470 (2018)
   PUBMED   30352685
REFERENCE   8  (bases 1 to 3798)
  AUTHORS   Morita,K., Hama,Y., Izume,T., Tamura,N., Ueno,T., Yamashita,Y.,
            Sakamaki,Y., Mimura,K., Morishita,H., Shihoya,W., Nureki,O.,
            Mano,H. and Mizushima,N.
  TITLE     Genome-wide CRISPR screen identifies TMEM41B as a gene required for
            autophagosome formation
  JOURNAL   J Cell Biol 217 (11), 3817-3828 (2018)
   PUBMED   30093494
  REMARK    GeneRIF: These results suggest that TMEM41B and VMP1 function
            together at an early step of autophagosome formation.
REFERENCE   9  (bases 1 to 3798)
  AUTHORS   Scaturro,P., Stukalov,A., Haas,D.A., Cortese,M., Draganova,K.,
            Plaszczyca,A., Bartenschlager,R., Gotz,M. and Pichlmair,A.
  TITLE     An orthogonal proteomic survey uncovers novel Zika virus host
            factors
  JOURNAL   Nature 561 (7722), 253-257 (2018)
   PUBMED   30177828
REFERENCE   10 (bases 1 to 3798)
  AUTHORS   Moretti,F., Bergman,P., Dodgson,S., Marcellin,D., Claerr,I.,
            Goodwin,J.M., DeJesus,R., Kang,Z., Antczak,C., Begue,D.,
            Bonenfant,D., Graff,A., Genoud,C., Reece-Hoyes,J.S., Russ,C.,
            Yang,Z., Hoffman,G.R., Mueller,M., Murphy,L.O., Xavier,R.J. and
            Nyfeler,B.
  TITLE     TMEM41B is a novel regulator of autophagy and lipid mobilization
  JOURNAL   EMBO Rep 19 (9) (2018)
   PUBMED   30126924
  REMARK    GeneRIF: TMEM41B is a novel ER-localized regulator of autophagosome
            biogenesis and lipid mobilization.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI829492.1, D26067.1 and
            AC055845.15.
            
            On Nov 22, 2018 this sequence version replaced NM_015012.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.477858.1,
                                           SRR1660807.127397.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000528080.6/ ENSP00000433126.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BI829492.1         157-175
            20-862              D26067.1           1-843
            863-3798            AC055845.15        126935-129870
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.4"
     gene            1..3798
                     /gene="TMEM41B"
                     /note="transmembrane protein 41B"
                     /db_xref="GeneID:440026"
                     /db_xref="HGNC:HGNC:28948"
                     /db_xref="MIM:620271"
     exon            1..273
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1028
                     /gene="TMEM41B"
                     /note="isoform 1 is encoded by transcript variant 1;
                     stasimon; protein stasimon"
                     /codon_start=1
                     /product="transmembrane protein 41B isoform 1"
                     /protein_id="NP_055827.1"
                     /db_xref="CCDS:CCDS31424.1"
                     /db_xref="GeneID:440026"
                     /db_xref="HGNC:HGNC:28948"
                     /db_xref="MIM:620271"
                     /translation="MAKGRVAERSQLGAHHTTPVGDGAAGTRGLAAPGSRDHQKEKSW
                     VEAGSARMSLLILVSIFLSAAFVMFLVYKNFPQLSEEERVNMKVPRDMDDAKALGKVL
                     SKYKDTFYVQVLVAYFATYIFLQTFAIPGSIFLSILSGFLYPFPLALFLVCLCSGLGA
                     SFCYMLSYLVGRPVVYKYLTEKAVKWSQQVERHREHLINYIIFLRITPFLPNWFINIT
                     SPVINVPLKVFFIGTFLGVAPPSFVAIKAGTTLYQLTTAGEAVSWNSIFILMILAVLS
                     ILPAIFQKKLKQKFE"
     exon            274..391
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     exon            392..520
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     exon            521..614
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     exon            615..719
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     exon            720..858
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     exon            859..3798
                     /gene="TMEM41B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3773..3778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM41B"
                     /note="hexamer: AATAAA"
     polyA_site      3798
                     /gene="TMEM41B"
                     /note="major polyA site"
ORIGIN      
        1 gcagtactcg ctgggctgag gtcgtctcgg gtgaggaggt cgcgccggaa gtggctctag
       61 ccgtggcgct tcggcgtagt aaggagcctg caacagagtt ttgttgttta gggggcgcgg
      121 caccgcaccg ctcccttcac cttgcagcag ccatggcgaa aggcagagtc gccgaacgat
      181 cgcagttggg cgctcaccac acgacccccg tgggggacgg ggcagcgggg acgcggggtc
      241 tcgcggcgcc tggcagcaga gaccaccaga aggaaaaatc ctgggtagaa gctggatcag
      301 caagaatgtc actccttata ttggtgtcca ttttcttatc tgcagctttt gttatgtttt
      361 tggtatataa aaattttcct cagcttagtg aagaagaaag agtgaatatg aaggttccca
      421 gagatatgga tgatgccaag gctctaggaa aagttttatc caaatacaag gacacctttt
      481 atgttcaagt acttgtagct tattttgcta catatatttt cttgcaaaca tttgctattc
      541 caggctctat atttctcagt atactctcag ggtttcttta tccctttcca ctagccttat
      601 ttcttgtttg tttgtgttct ggacttggtg cctctttctg ttatatgctt tcctatttag
      661 ttgggagacc agttgtatac aaatacctaa cagagaaagc agtaaaatgg tcacagcagg
      721 ttgaacgtca tagagaacat ctcattaact acattatatt tttgagaata acaccatttc
      781 tgcctaattg gtttattaat atcacatctc ctgtgataaa cgtgccattg aaagtttttt
      841 ttattggtac ttttctaggt gtcgcacctc cttcttttgt agccattaag gcaggaacaa
      901 cactgtatca acttacaaca gcaggagaag ctgtttcctg gaactcaata tttattctga
      961 tgatcttggc tgttctttct attctgccag ccatcttcca aaaaaaacta aagcagaaat
     1021 ttgagtaaaa ataatcatct gatttttagt tttgctgtca tcaccatctc aggattaaca
     1081 ggtgcatcca tttatgtttt aaaatcacct cttcagtaat taaacaagga atttgtaaaa
     1141 taaaatttcc tatcagatag ttaaagtaat acatttaagt gacaagggga gaagaaagta
     1201 aaaatggaaa ttggtatact gtgaaaagtg ctatcaatag ttaaggtaga catcttagac
     1261 atcttataaa ttattatacc aagggtagct gtagataata gtagtgtggt aggaaattgt
     1321 tgatccagct aattcattca atttaattga atatttttta tctttaataa ctaaaataga
     1381 tatttttaaa ttctagattt tttaaagtag tgttgcaggt tttgttaatg ttaggctttt
     1441 ttcagagcat aaactggaaa acttaaaaag agatttacat tttcttcctc tttttttttt
     1501 tttttttttt tctgttttga gacggagtct agctctgccg cccaggctgg agtgcagtgg
     1561 cgcgatctcc actcactgca agctccacct cctgggttca cgccattctc ctgtctcagc
     1621 ctcctgagta gctgggacta caggcgcccg ccaccatgcc cagctaattt ttttgtattt
     1681 ttagtagaga cggggtttca ccgtgttagc caggatggtc tcgatctcct gacctcgtga
     1741 tctgcccgcc ttggcctccc aaagtgctgg gattacaggc gtgagccacc gcgcctggcc
     1801 cattttcttc ctcttttgag gtaatggatt tgtttggaga tggcatgtta gtagacgact
     1861 gaatatggaa aggatatcaa gttatctatt ttgttaattt tatttttgtt ttttatcatc
     1921 tagattttta tcatggatta gtctgaaatt taaagttctg gccagtcggt tttctttcat
     1981 cttgtagttt ttacagtatt tccactgtgc atatgcaaaa tgggtattac ataactgtat
     2041 catatttggt attgataatt tttttttttt ttgagacgga gtcttgctgt tgcccaggct
     2101 ggagtggagt gcgtggtgtg atctcggctc actgcaaact ccgcctcctg ggttcaagcg
     2161 attctcctgg cccagcctcc tgagtagctg ggattacagg tgtgtgccac cgtgcccagc
     2221 taatttttgt gtttttagta gagacggggt ttcaccatgt tggccgggct ggtcttgaac
     2281 tcctgacctc aggctatatg cccacttcca cctcccaaag tgctgggatt acaggcatga
     2341 gccactgtgc ccagcctggt attgataatt tatattcaga taatttgtta tggctcttta
     2401 atatcccaca aggggctcta aaaagcaaac attcaagagt atgtagtttt tagacattaa
     2461 gttaattatt ttaaacagtg acagcaaaac acaagtgatt aaatatagtt tatttgttcc
     2521 aatgactaaa ttttacctca tttattaatc tggtcattaa ggaatatatt taataatatt
     2581 atgtaattat tctttttatg catgatacac ctagaaaaat gccttttgtt tctattgatg
     2641 gctttgttgt ttggagctac ttttgattac ttattgcagt ttcccaattt agtctttact
     2701 ttatctaact cacaaagtaa aattaactga tcacatggca actactgtat ttaaatagtt
     2761 ctggaaaaat gaaagtgctt tttgctgctt ggtaaatggg taatgccctt gattccttga
     2821 ctgtaggaca tagctgatct aaagtactct gtcagtttta ccttcaccca tgactgttat
     2881 tagttgtcaa agttgaaaag tactttagct gtgagaaatc cttgtatgtt tttattataa
     2941 gaggtataat catcctcaaa gcctgttttt attacatgat gtggactgat tattttttct
     3001 atcacagtgt taacagatgg attttattgt aaatacaaag aaaacatatt gattattgta
     3061 gtattcttat gtcacctggc cttttgcgtg agattattta ttatttctag caaggctttc
     3121 ttcctttctt attgcccaga gactgactga tacatctttt gttattttta cacataaatt
     3181 aaacatagcc tttttggaca aattcactaa atattaatgt ataaaatgta attgagtaaa
     3241 tttttatcag aattttaaaa ataaaagagc ttagactcag tagaactcag tagaagcttc
     3301 actatttact ccagcgtgtg taaattgtac ttactctatt ctcagagtat atttactgtc
     3361 cttaccattg attctttccc tttgctaatt tttttttttg ttaatggtag ctgcgacttt
     3421 aggtggggta tattttcttc tcctaagaga atagacagtt tttccagatt catcatcatt
     3481 gactgtcaag aaaggaccct tcagcaaggc tgtaccctca atgcagttga tggcctgtct
     3541 tcacggattt acagacttgg cctgatgccc atgtaaattc aagctttggc ttgtggtaac
     3601 aaccacaaga agacaagcat ctgtggtgcg gaggcaagca ggctaactag gagttgacaa
     3661 gctaagaaag tgaaactgtt ctttcttagt taactgtctt tctctggagc tctgttattt
     3721 tgagtataat atttccatga cacttagtaa atgcaagcta aaatgtaata ataataaatt
     3781 gtattggaga aacctaaa
//
