LOCUS       NM_021637               2039 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens transmembrane protein 35A (TMEM35A), mRNA.
ACCESSION   NM_021637
VERSION     NM_021637.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wu,M., Liu,C.Z., Barrall,E.A., Rissman,R.A. and Joiner,W.J.
  TITLE     Unbalanced Regulation of alpha7 nAChRs by Ly6h and NACHO
            Contributes to Neurotoxicity in Alzheimer's Disease
  JOURNAL   J Neurosci 41 (41), 8461-8474 (2021)
   PUBMED   34446574
  REMARK    GeneRIF: Unbalanced Regulation of alpha7 nAChRs by Ly6h and NACHO
            Contributes to Neurotoxicity in Alzheimer's Disease.
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Mazzaferro,S., Whiteman,S.T., Alcaino,C., Beyder,A. and Sine,S.M.
  TITLE     NACHO and 14-3-3 promote expression of distinct subunit
            stoichiometries of the alpha4beta2 acetylcholine receptor
  JOURNAL   Cell Mol Life Sci 78 (4), 1565-1575 (2021)
   PUBMED   32676916
  REMARK    GeneRIF: NACHO and 14-3-3 promote expression of distinct subunit
            stoichiometries of the alpha4beta2 acetylcholine receptor.
REFERENCE   3  (bases 1 to 2039)
  AUTHORS   Kweon,H.J., Gu,S., Witham,E., Dhara,M., Yu,H., Mandon,E.D.,
            Jawhari,A. and Bredt,D.S.
  TITLE     NACHO Engages N-Glycosylation ER Chaperone Pathways for alpha7
            Nicotinic Receptor Assembly
  JOURNAL   Cell Rep 32 (6), 108025 (2020)
   PUBMED   32783947
REFERENCE   4  (bases 1 to 2039)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2039)
  AUTHORS   Deshpande,A., Vinayakamoorthy,R.M., Garg,B.K., Thummapudi,J.P.,
            Oza,G., Adhikari,K., Agarwal,A., Dalvi,P., Iyer,S., Thulasi
            Raman,S., Ramesh,V., Rameshbabu,A., Rezvaya,A., Sukumaran,S.,
            Swaminathan,S., Tilak,B., Wang,Z., Tran,P.V. and Loring,R.H.
  TITLE     Why Does Knocking Out NACHO, But Not RIC3, Completely Block
            Expression of alpha7 Nicotinic Receptors in Mouse Brain?
  JOURNAL   Biomolecules 10 (3), 470 (2020)
   PUBMED   32204458
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2039)
  AUTHORS   Matta,J.A., Gu,S., Davini,W.B., Lord,B., Siuda,E.R.,
            Harrington,A.W. and Bredt,D.S.
  TITLE     NACHO Mediates Nicotinic Acetylcholine Receptor Function throughout
            the Brain
  JOURNAL   Cell Rep 19 (4), 688-696 (2017)
   PUBMED   28445721
REFERENCE   7  (bases 1 to 2039)
  AUTHORS   Rex,E.B., Shukla,N., Gu,S., Bredt,D. and DiSepio,D.
  TITLE     A Genome-Wide Arrayed cDNA Screen to Identify Functional Modulators
            of alpha7 Nicotinic Acetylcholine Receptors
  JOURNAL   SLAS Discov 22 (2), 155-165 (2017)
   PUBMED   27789755
REFERENCE   8  (bases 1 to 2039)
  AUTHORS   Gu,S., Matta,J.A., Lord,B., Harrington,A.W., Sutton,S.W.,
            Davini,W.B. and Bredt,D.S.
  TITLE     Brain alpha7 Nicotinic Acetylcholine Receptor Assembly Requires
            NACHO
  JOURNAL   Neuron 89 (5), 948-955 (2016)
   PUBMED   26875622
REFERENCE   9  (bases 1 to 2039)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL109952.15, BC078658.1 and BU736545.1.
            
            On Nov 22, 2018 this sequence version replaced NM_021637.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The extent of this RefSeq transcript is based on
            transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223240.1, AK024146.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372930.5/ ENSP00000362021.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL109952.15        44072-44074
            4-983               BC078658.1         1-980
            984-2035            AL109952.15        60505-61556
            2036-2039           BU736545.1         16-19               c
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     gene            1..2039
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /note="transmembrane protein 35A"
                     /db_xref="GeneID:59353"
                     /db_xref="HGNC:HGNC:25864"
     exon            1..244
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..628
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /note="transmembrane protein 35; nAChR regulator;
                     nicotinic acetylcholine receptor regulator"
                     /codon_start=1
                     /product="novel acetylcholine receptor chaperone"
                     /protein_id="NP_067650.1"
                     /db_xref="CCDS:CCDS14478.1"
                     /db_xref="GeneID:59353"
                     /db_xref="HGNC:HGNC:25864"
                     /translation="MASPRTVTIVALSVALGLFFVFMGTIKLTPRLSKDAYSEMKRAY
                     KSYVRALPLLKKMGINSILLRKSIGALEVACGIVMTLVPGRPKDVANFFLLLLVLAVL
                     FFHQLVGDPLKRYAHALVFGILLTCRLLIARKPEDRSSEKKPLPGNAEEQPSLYEKAP
                     QGKVKVS"
     exon            245..2039
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2012..2017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /note="hexamer: AATAAA"
     polyA_site      2039
                     /gene="TMEM35A"
                     /gene_synonym="NACHO; TMEM35; TUF-1"
                     /note="major polyA site"
ORIGIN      
        1 gtcattctag ctgcctgctg cctccgcagc gtccccccag ctctccctgt gctaactgcc
       61 tgcaccttgg acagagcggg tgcgcaaatc agaaggatta gttgggacct gccttggcga
      121 ccccatggca tcccccagaa ccgtaactat tgtggccctc tcagtggccc tgggactctt
      181 ctttgttttc atggggacta tcaagctgac ccccaggctc agcaaggatg cctacagtga
      241 gatgaaacgt gcttacaaga gctatgttcg agccctccct ctgctgaaga aaatggggat
      301 caattccatt ctcctccgaa aaagcattgg tgcccttgaa gtggcctgtg gcatcgtcat
      361 gacccttgtg cctgggcgtc ccaaagatgt ggccaacttc ttcctactgt tgctggtgtt
      421 ggctgtgctc ttcttccacc agctggtcgg tgatcctctc aaacgctacg cccatgctct
      481 ggtgtttgga atcctgctca cttgccgcct gctgattgct cgcaagcccg aagaccggtc
      541 ttctgagaag aagcctttgc cagggaatgc tgaggagcaa ccctccttat atgagaaggc
      601 ccctcagggc aaagtgaagg tgtcatagaa aagtggaagt gcaaagagtg gaccttccag
      661 gcagttgcgt ccatgacacc aggaagatgt cagtgtgtgt ttttcatttg atttatttat
      721 cttggggaaa gtgaaaaatg taatctgcaa gttaatgacc ctattggctt gtgtacatct
      781 atatgctaaa atgacttccc cacattgaca tttgtgcgcc acctttaatc actctggggc
      841 aactctcaca tcttgctgca tgtacatgta tacggctact attgaagtgt aattgtgaga
      901 tggactccaa caagcatgtg actgtgagat tgtgtgtggg aaaatgtatt taactactct
      961 gtgtgtgtgt gtgtgtgtgt gtgcgcgcgc gcgcgcacgc gcacacactc acgcacacac
     1021 aagcagagaa ggcgctgatc ttgaactaat cctgcacagg catccttccc tttatagatt
     1081 gattccagca aaggcggaat aaaacaaatt tcctatgaag agaatcctga tatgaaacaa
     1141 gtcatgtagt ctcatggccg ggaatctctc cacagatact aacaacttaa acttactact
     1201 ttaggagaaa aaaaaaaaca ttcaatttcg gacactgagt tatatatgaa attaattagg
     1261 ctctagtcca acagttgttt acattttaaa tagtccatat tgaatttaat taaaacaagg
     1321 gatgcatgca gtcaaattga tagtttaatt cttcaagtga taatatagga agtttcacct
     1381 tgcctttgtc caagccccac ctattaaaac cctttactca cagtttgaaa ctgaagcagt
     1441 aaacttgttt ccagacatct ttttcagatt gtcttaagcc caaagttgcc tcacttccac
     1501 tattctcagc agccaaccag gatttggcag ctgctccact gttacggttg agggaacagg
     1561 gatcagtcct gttagaagtc tgtgagcctc aaactctacc tgttctctgc aatcatccaa
     1621 aatttgaaaa agaagctata tccagtgttt cactgccaaa cagattcact actcttactg
     1681 attcttcact gagctttgct agtataagca gagttccaag tctcccctag ggttgtctct
     1741 acatttcttt atcattccag tgggtagggt ttagctgggg gaaggacatt tcataagggt
     1801 tagttggact gagcagtatg gacatttgct tttttcatta cgtactgttg tttttccttg
     1861 ttaggtgtgc tttggtggtt ttaatattat tgtgccaggg atggggaaat ggggggggtt
     1921 gtgtgggaag agtacttatt attgtgtttt cttcagtgta attgttcttg gtaattgata
     1981 cctctctgtt ttatttctct cattctttca aaataaaact ttttgaaatt tggaggaaa
//
