LOCUS       NM_001280561             918 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens transmembrane protein 249 (TMEM249), transcript
            variant 4, mRNA.
ACCESSION   NM_001280561
VERSION     NM_001280561.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc Natl Acad Sci U S A 99 (26), 16899-16903 (2002)
   PUBMED   12477932
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC233992.5.
            
            On Oct 11, 2020 this sequence version replaced NM_001280561.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 4 encode the same isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB196634.1, SRR5189655.222679.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000696146.1/ ENSP00000512438.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                AC233992.5         117154-117243       c
            91-241              AC233992.5         116929-117079       c
            242-336             AC233992.5         116549-116643       c
            337-532             AC233992.5         116266-116461       c
            533-618             AC233992.5         116090-116175       c
            619-918             AC233992.5         115540-115839       c
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..918
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /note="transmembrane protein 249"
                     /db_xref="GeneID:340393"
                     /db_xref="HGNC:HGNC:44155"
     exon            1..90
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..798
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /note="isoform 1 is encoded by transcript variant 4;
                     putative transmembrane protein C8orfK29; cation channel
                     sperm-associated auxiliary subunit TMEM249"
                     /codon_start=1
                     /product="cation channel sperm-associated auxiliary
                     subunit TMEM249 isoform 1"
                     /protein_id="NP_001267490.1"
                     /db_xref="CCDS:CCDS59117.1"
                     /db_xref="GeneID:340393"
                     /db_xref="HGNC:HGNC:44155"
                     /translation="MPKGRAGSLPTTSIGWRFQLWFLGLTCPERHLARRLKNNSFYPF
                     VQQEPNVFVLEYYLDTLWKGMLLFIISVVLVSFSSLREVQKQETWVFLVYGVGVGLWL
                     VISSLPRRRLVLNHTRGVYHFSIQGRTVCQGPLHLVYVRLALSSDAHGRCFFHLVLGG
                     HRLEPLVLVQLSEHYEQMEYLGRYIARKLNINYFDYLATSYRHVVRHWPPPGAGTVMG
                     KSPMGHKPSSSQSSLEV"
     exon            91..241
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     exon            242..336
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     exon            337..532
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     exon            533..618
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     exon            619..918
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /inference="alignment:Splign:2.1.0"
     regulatory      899..904
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /note="hexamer: AATAAA"
     polyA_site      918
                     /gene="TMEM249"
                     /gene_synonym="C8ORFK29"
                     /note="major polyA site"
ORIGIN      
        1 gggccgctcc ctggcctcgc tgccctgtca gtcgcctgtg agctcctgct ccttcctggg
       61 cctgcagccc tggagaattt gctcaccgag atgcctaagg gccgggccgg cagcctgccc
      121 acgacctcca tcggctggcg cttccagctc tggttcctag gactcacctg cccggagcgc
      181 cacctggcca gacgcctcaa gaacaatagc ttctacccat tcgtgcagca ggagccaaac
      241 gtcttcgtgc tcgaatacta cctggacaca ctgtggaagg ggatgctgct cttcatcatc
      301 agcgtggtcc tggtcagctt cagctccctg agagaggtgc agaagcaaga gacctgggtc
      361 ttcctcgtgt acggcgtggg cgtgggcctg tggctcgtga tctcgtcgct gccgcggcgc
      421 cgcctggtgc tgaaccacac gcgcggcgtg taccacttct ccatccaggg ccgcaccgtg
      481 tgccagggcc ccttgcatct ggtctacgtg cgcctggcgc tcagctccga tgcccacgga
      541 aggtgcttct tccacctggt cctcggcggc cacaggttgg agcccctggt gctggtgcag
      601 ctgtcggagc actacgagca aatggaatac ttgggccgtt acatcgcccg gaaactcaac
      661 atcaactact tcgactacct ggccacctcc taccggcacg tggttcgcca ctggccaccc
      721 cctggcgctg gcactgtgat gggcaagagc cccatgggtc acaagcccag ctcctctcag
      781 tccagcctgg aggtgtgact ctgagggcct cccccacctc gggactgtgt tctcggtaac
      841 ctcccagccc cactctgggg cagatcaaat gtgagtccca ctcactggcc ttcctttcaa
      901 taaagctgcc tcagcttc
//
