LOCUS       NM_022484               7276 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens transmembrane protein 168 (TMEM168), transcript
            variant 2, mRNA.
ACCESSION   NM_022484
VERSION     NM_022484.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Nguyen,L.K.C., Shimizu,A., Soh,J.E.C., Komeno,M., Sato,A. and
            Ogita,H.
  TITLE     Transmembrane protein 168 mutation reduces cardiomyocyte cell
            surface expression of Nav1.5 through alphaB-crystallin
            intracellular dynamics
  JOURNAL   J Biochem 170 (5), 577-585 (2021)
   PUBMED   34086898
  REMARK    GeneRIF: Transmembrane protein 168 mutation reduces cardiomyocyte
            cell surface expression of Nav1.5 through alphaB-crystallin
            intracellular dynamics.
            Erratum:[J Biochem. 2022 Mar 31;171(4):467. doi:
            10.1093/jb/mvab152.. PMID: 35137094]
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Shimizu,A., Zankov,D.P., Sato,A., Komeno,M., Toyoda,F.,
            Yamazaki,S., Makita,T., Noda,T., Ikawa,M., Asano,Y., Miyashita,Y.,
            Takashima,S., Morita,H., Ishikawa,T., Makita,N., Hitosugi,M.,
            Matsuura,H., Ohno,S., Horie,M. and Ogita,H.
  TITLE     Identification of transmembrane protein 168 mutation in familial
            Brugada syndrome
  JOURNAL   FASEB J 34 (5), 6399-6417 (2020)
   PUBMED   32175648
  REMARK    GeneRIF: Identification of transmembrane protein 168 mutation in
            familial Brugada syndrome.
REFERENCE   3  (bases 1 to 7276)
  AUTHORS   Xu,J., Su,Z., Ding,Q., Shen,L., Nie,X., Pan,X., Yan,A., Yan,R.,
            Zhou,Y., Li,L. and Lu,B.
  TITLE     Inhibition of Proliferation by Knockdown of Transmembrane (TMEM)
            168 in Glioblastoma Cells via Suppression of Wnt/beta-Catenin
            Pathway
  JOURNAL   Oncol Res 27 (7), 819-826 (2019)
   PUBMED   30940290
  REMARK    GeneRIF: Data suggested that siRNA-TMEM168 (siTMEM168) prevented
            viability of U87 and U373 cells, induced cell cycle arrest (G0/G1
            phase) and promoted apoptosis, and the mechanisms involved in
            blocking Wnt/beta-catenin pathway, as evidenced by reducing
            expression of beta-catenin, C-myc, cyclin D1, and survivin.
REFERENCE   4  (bases 1 to 7276)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 7276)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC416793.1, AK314460.1, AC079621.7 and AI698728.1.
            
            On May 17, 2019 this sequence version replaced NM_022484.5.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.172457.1, AL136626.2
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312814.11/ ENSP00000323068.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DC416793.1         31-33
            4-2456              AK314460.1         1-2453
            2457-6803           AC079621.7         39376-43722         c
            6804-7271           AI698728.1         18-485              c
            7272-7276           AC079621.7         38903-38907         c
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.1"
     gene            1..7276
                     /gene="TMEM168"
                     /note="transmembrane protein 168"
                     /db_xref="GeneID:64418"
                     /db_xref="HGNC:HGNC:25826"
     exon            1..234
                     /gene="TMEM168"
                     /inference="alignment:Splign:2.1.0"
     exon            235..1490
                     /gene="TMEM168"
                     /inference="alignment:Splign:2.1.0"
     CDS             363..2456
                     /gene="TMEM168"
                     /codon_start=1
                     /product="transmembrane protein 168"
                     /protein_id="NP_071929.3"
                     /db_xref="CCDS:CCDS5757.1"
                     /db_xref="GeneID:64418"
                     /db_xref="HGNC:HGNC:25826"
                     /translation="MCKSLRYCFSHCLYLAMTRLEEVNREVNMHSSVRYLGYLARINL
                     LVAICLGLYVRWEKTANSLILVIFILGLFVLGIASILYYYFSMEAASLSLSNLWFGFL
                     LGLLCFLDNSSFKNDVKEESTKYLLLTSIVLRILCSLVERISGYVRHRPTLLTTVEFL
                     ELVGFAIASTTMLVEKSLSVILLVVALAMLIIDLRMKSFLAIPNLVIFAVLLFFSSLE
                     TPKNPIAFACFFICLITDPFLDIYFSGLSVTERWKPFLYRGRICRRLSVVFAGMIELT
                     FFILSAFKLRDTHLWYFVIPGFSIFGIFWMICHIIFLLTLWGFHTKLNDCHKVYFTHR
                     TDYNSLDRIMASKGMRHFCLISEQLVFFSLLATAILGAVSWQPTNGIFLSMFLIVLPL
                     ESMAHGLFHELGNCLGGTSVGYAIVIPTNFCSPDGQPTLLPPEHVQELNLRSTGMLNA
                     IQRFFAYHMIETYGCDYSTSGLSFDTLHSKLKAFLELRTVDGPRHDTYILYYSGHTHG
                     TGEWALAGGDTLRLDTLIEWWREKNGSFCSRLIIVLDSENSTPWVKEVRKINDQYIAV
                     QGAELIKTVDIEEADPPQLGDFTKDWVEYNCNSSNNICWTEKGRTVKAVYGVSKRWSD
                     YTLHLPTGSDVAKHWMLHFPRITYPLVHLANWLCGLNLFWICKTCFRCLKRLKMSWFL
                     PTVLDTGQGFKLVKS"
     exon            1491..1633
                     /gene="TMEM168"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1908
                     /gene="TMEM168"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..7276
                     /gene="TMEM168"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3899..3904
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM168"
                     /note="hexamer: AATAAA"
     polyA_site      3921
                     /gene="TMEM168"
                     /note="major polyA site"
     regulatory      6238..6243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM168"
                     /note="hexamer: AATAAA"
     regulatory      6260..6265
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM168"
                     /note="hexamer: AATAAA"
     polyA_site      6265
                     /gene="TMEM168"
     polyA_site      6285
                     /gene="TMEM168"
     regulatory      6627..6632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM168"
                     /note="hexamer: ATTAAA"
     polyA_site      6654
                     /gene="TMEM168"
ORIGIN      
        1 gcggatttcg gcggcggaaa catggcggtc gcggccgggc cggtaacgga gaaagtttac
       61 gccgacactg gcctgtatta gcgcgtatgg cctcgggccc tcgttcccca aggcgtgccg
      121 cctccctgtt ctcagtcgca ggctgaagcc ttgtctgctc tcctcctttt tggtttggtt
      181 ttggaactga ctccgagggt tgggagagcg cgttggtggc gacggccgag tcagatcact
      241 ataaacaaaa tttccacaag agaaaatgtt gaaataggag ttgcggatac attggatata
      301 ctggatgaaa tacaagcggt taatttttgt aacgtgaggg aaaagcccac attgctggtt
      361 acatgtgtaa atcactgcgt tattgcttta gtcattgtct ctatttagca atgacaagac
      421 tggaagaagt aaatagagaa gtgaacatgc attcttcagt gcggtatctt ggctatttag
      481 ccagaatcaa tttattggtt gctatatgct taggtctata cgtaagatgg gaaaaaacag
      541 caaattcctt aattttggta atttttattc ttggtctttt tgttcttgga atcgccagca
      601 tactctatta ctatttttca atggaagcag caagtttaag tctctccaat ctttggtttg
      661 gattcttgct tggcctccta tgttttcttg ataattcatc ctttaaaaat gatgtaaaag
      721 aagaatcaac caaatatttg cttctaacat ccatagtgtt aaggatattg tgctctctgg
      781 tggagagaat ttctggttat gtccgtcatc ggcccacttt actaaccaca gttgaatttc
      841 tggagcttgt tggatttgcc attgccagca caactatgtt ggtggagaag tctctgagtg
      901 tcattttgct tgttgtagct ctggctatgc tgattattga tctgagaatg aaatctttct
      961 tagctattcc aaacttagtt atttttgcag ttttgttatt tttttcctca ttggaaactc
     1021 ccaaaaatcc gattgctttt gcgtgttttt ttatttgcct gataactgat cctttccttg
     1081 acatttattt tagtggactt tcagtaactg aaagatggaa accctttttg taccgtggaa
     1141 gaatttgcag aagactttca gtcgtttttg ctggaatgat tgagcttaca ttttttattc
     1201 tttccgcatt caaacttaga gacactcacc tctggtattt tgtaatacct ggcttttcca
     1261 tttttggaat tttctggatg atttgtcata ttatttttct tttaactctt tggggattcc
     1321 ataccaaatt aaatgactgc cataaagtat attttactca caggacagat tacaatagcc
     1381 ttgatagaat catggcatcc aaagggatgc gccatttttg cttgatttca gagcagttgg
     1441 tgttctttag tcttcttgca acagcgattt tgggagcagt ttcctggcag ccaacaaatg
     1501 gaattttctt gagcatgttt ctaatcgttt tgccattgga atccatggct catgggctct
     1561 tccatgaatt gggtaactgt ttaggaggaa catctgttgg atatgctatt gtgattccca
     1621 ccaacttctg cagtcctgat ggtcagccaa cactgcttcc cccagaacat gtacaggagt
     1681 taaatttgag gtctactggc atgctcaatg ctatccaaag attttttgca tatcatatga
     1741 ttgagaccta tggatgtgac tattccacaa gtggactgtc atttgatact ctgcattcca
     1801 aactaaaagc tttcctcgaa cttcggacag tggatggacc cagacatgat acgtatattt
     1861 tgtattacag tgggcacacc catggtacag gagagtgggc tctagcaggt ggagatacac
     1921 tacgccttga cacacttata gaatggtgga gagaaaagaa tggttccttt tgttcccggc
     1981 ttattatcgt attagacagc gaaaattcaa ccccttgggt gaaagaagtg aggaaaatta
     2041 atgaccagta tattgcagtg caaggagcag agttgataaa aacagtagat attgaagaag
     2101 ctgacccgcc acagctaggt gactttacaa aagactgggt agaatataac tgcaactcca
     2161 gtaataacat ctgctggact gaaaagggac gcacagtgaa agcagtatat ggtgtgtcaa
     2221 aacggtggag tgactacact ctgcatttgc caacgggaag cgatgtggcc aagcactgga
     2281 tgttacactt tcctcgtatt acatatcccc tagtgcattt ggcaaattgg ttatgcggtc
     2341 tgaacctttt ttggatctgc aaaacttgtt ttaggtgctt gaaaagatta aaaatgagtt
     2401 ggtttcttcc tactgtgctg gacacaggac aaggcttcaa acttgtcaaa tcttaatttg
     2461 gaccccaaag cgggatatta ataagcactc atactaccaa ttatcactaa cttgccattt
     2521 tttgtatgct gtatttttat ttgtggaaaa taccttgcta cttctgtagc tgctctcact
     2581 ttgtcttttc ttaagtaatt atggtatata taaggcgttg ggaaaaaaca ttttataatg
     2641 aaagtatgta gggagtcaaa tgcttactgt aaatgcataa gagacgttaa aaataacact
     2701 gcactttcag gaatgtttgc ttatggtcct gattagaaag aaacagttgt ctatgctctg
     2761 caatggtcaa tgatgaatta ctaatgcctt attttctagg catataataa tagtttagag
     2821 aatgtagacc agataaattt gtttactgtt ttaagaaaac taccagttta cttacagaag
     2881 attctttttt ccaaacagta ggtttcatcc aagaccattt gaagaactgc aaactctttc
     2941 tcttagaaaa gaaagagggc agcctaaaat aaacgcaaaa tttgcttata ctccatcaca
     3001 ttcagatgtc ttggttgtga cttattacca gtgtggcaga gaacccaagt tacattttag
     3061 atcaaaatat tctttatgta ggtattgtta aaaggctaga gcctacaagt tgctcttcca
     3121 tgcgttggtc agggggccct gaaaacactg gtaatattaa gagtctttct cagggtaact
     3181 taatgttttc ttaatgaaca gtgtttccag ctacaaattc ttccaataaa ttgtcttcct
     3241 ttttgaaaag tactctcata gaagaaattt agcaatttct cgttgactga ctcagtctat
     3301 tttaagtatt cagaaaagat tttgatcccc attgagttaa tgctctgcct tgaaaattat
     3361 ttttctgatc cttgttagtg ataacatttt ttttctactg aaggtcagag gataggaaac
     3421 aagtatttct cttctggtat acatgtaatg tattctgtaa aaaagtattc atattggcaa
     3481 ttttagttag gcataatatt gtggttgtaa tttttaaaac ttagtgtttt gtctgattaa
     3541 agcaggcact gatcagggta tctcctaaga ggtaattcac ttcttattcc tttccaataa
     3601 ttattacatt ctaaattttc atctatgaga aataacaaac aagaagggaa tagaattaaa
     3661 ttggggtata atctaatctt cattgtttaa atggtttgcc ttctcaccat tgaagccatt
     3721 tttttatagc ctcagaaaga ggaaataatg cctccaccat tttctacctg gtgacttgaa
     3781 aattgaactt ttaagttagg aagaagttag agtcagggaa cttgtatacc actatctatg
     3841 cagcattgtt atagtctgat tatttctgtg ttttgaatat gattttccta atgctctaaa
     3901 taaaattttg ttaaaaatta attttttatt taatgatgtg caaatattga atattttagt
     3961 atatttatta aaagtggtag tcattaaaaa tgcccatgtt ttagagttca tgtttggaag
     4021 tttccattat aggttaagga tttatattag tattgtccaa ataaaatagt atagtttttt
     4081 ttattgctct gttcagcagt agggtagtag aaaagccaaa tgttaaaatt atgagctttt
     4141 agcttaaaaa aagttaatgt taagtaaaat ctccttttaa atttctcagc tatttgtgag
     4201 caggttagct ttaaagcatt atctcttcac tttaagaaag ccaaatacaa aagtatattc
     4261 tgcattcttt ataaggtagt attaaggtgg tactaaaaat tacattttaa aaacagctga
     4321 cagtttgaca gccacaaacc taactttttc atatgtattt cctgtgaaag taaaaccaaa
     4381 aatggtcttt gggccaagaa tgtgacttga gccaatttgt taaccaaaaa tttatagaat
     4441 gtccactttg atgaattctc ctacccaaac aaacaaaaaa caataaatga ctttttggga
     4501 tggtatcaca aagctaacat tttaaaaaca ataactaggc cgggtgcagt ggctcacgcc
     4561 tgtaatccca gcactttggg aggcctaggt gggtggatca cttgaggcca ggagtttgag
     4621 accagcctgg ccaacatggg gaaacctcat cgctactaaa aatacaaaaa aaattattag
     4681 ccgggcatgg tggcaggtgc ctgtaatccc agctacttgg gaggctaagg cacaagaatc
     4741 gcttgaaccc gggaggcaga ggttgcagtg agctgagatt gtgccactgc actccagcct
     4801 gggcgacaga gcaggactct ttctttgtct caaaaaaaaa aaaaagtaca tgtttagcca
     4861 ggcatggtgg ctcatgcctg taatcccagc actttgggag gccgaggcag gtggatcaaa
     4921 aggtcaagag atggagacca tctggccaac atggtgaaac cccatctcta ctaaaaataa
     4981 aaaaatcagc tgggcgtggt ggtgcacgcc tgtagtccca tctacttggg agactgaggc
     5041 aggagaatct cttgaaccgg ggaggcagag gttgcagtga gccgagattg cgccactgca
     5101 ctccagcctg gcaacagaga gagactccat ctcaaaaaaa aaaagaaaca aacaaaaaaa
     5161 ataataaggg tgtagaaaag aaatattaca agaagaataa agtgattggt tactttcacc
     5221 taaagattga aaaatagatc tgacaatgag actaatttgt ctatggctgt catgtttaag
     5281 aaagagttgc ttggagtgaa aggaattatt ttagggcaaa actttaccat tctactaaaa
     5341 agaggtaaca tggagatttt ttcaacataa ctttgtttag tatttgtttc ctcatgtgat
     5401 accttgctaa aaccacaaaa ccactagctc taaaaacatc atcttttttt tcaatagaat
     5461 aaaaaaaaca gtatgaattt attttctttt tttaaccatt atttatttat tttaatttta
     5521 ttattattat actttaagtt ttagggtaca tgtgcaaata gcatacacac tgttgcaaaa
     5581 taaataagga gccaagttct ttctctcccc atgacttttc agttatgcca tcattttatt
     5641 caaaaatttt tagtttgctc tgatctaaac tcttccctgg gaaacttggg gttatatttc
     5701 catagaattc tgtcttgctc ttaataaatt caaaattctt tggaaaagat gcatttcaga
     5761 ggattattct tatgaccagt tatgctttaa aaaaattaaa gtcttaatta aaatgcactt
     5821 atatttaatg cattagtggg tagatttttg ttttgagtaa aatctttatt agaatctatt
     5881 tgtaactgga tattttgtaa ttttgggctc aaacatattt actcttgggt actgcttttt
     5941 tatatgtatt caaatttata gcttagtctc ttttaaagta atattctaca ttactttccc
     6001 acaagtgatg atagttaata taatgtatgc cagaagaaaa tgatttaatt ttaagaatgt
     6061 gattagatat atttgacagt aagtgctcaa aggagttaga tctgtcagta ctttcgaatg
     6121 tctcctgtat tggaaactat tagactgtca cctcttttta ggcagaggct tacacatgtt
     6181 ggtatttctg gtacacagtt accgagaaga gtgccttgtc cgtaatgaga agcatttaat
     6241 aaatattttg gactgagtga ataaatctag aatcctaagg agaaattgga ataacttgat
     6301 tgtaaattac tacattttct ttagttttac ttaaatggat attatttcaa gatcttcatt
     6361 aagcaagtaa tttagtaaaa aacttacaga atccataaaa agaaagcaaa tctcaaattt
     6421 gtagttaaga gtaataagtg ttaagattag ggaaggaaaa actgtttcag aactggtttg
     6481 aatgaaaaca tagctgatgt tgccaaaaaa tggctttttg tgagttgagt ccatttccat
     6541 catgactcta atatcattaa gattgaatca ttcatttcct cactattata tctagacaga
     6601 ttggaaattt accagaaatg gaaattatta aaagtctgac tgatgaacaa agaaggaatt
     6661 tgactatgca tatcagtgac aactaaattg gaaggtcagt aaaataggcc tataagcata
     6721 agcaggttaa ggacaagcta gggaaactat aagtatctcc ttttttatat tgtcttacct
     6781 attcactaat tataacacat agaaacaata taaaacgatg aagtttaagc agtaattgtt
     6841 ttgagaaacc attctgttga taatttacat ttatgtttat taggaaatat tgttattatt
     6901 acttagacca aagttgactg aaaattgaaa actgtgggct acccgtaagg ttttttgctt
     6961 tgaaatattc aatacctaaa agccattttg attttaaaaa tgaagttgac cagtcatctt
     7021 gtatgatgac ctcataatat tttataagtt tgaacttaaa atagttagaa gtagacagtt
     7081 ttagtaagct tttgttttta tttggtctag cctaaaagac attgatttat ctcaaagatt
     7141 tttagcagtg tgcaagggac aaatgagtat gacatagctt cctagacaag cagtgttcac
     7201 tcaatgccaa gtaaactttt ctaagttcaa aaatattttt atattaaaac acataccaat
     7261 gtattgatga acaaaa
//
