LOCUS       NM_001080825            7510 bp    mRNA    linear   PRI 02-JUN-2024
DEFINITION  Homo sapiens transmembrane protein 120B (TMEM120B), mRNA.
ACCESSION   NM_001080825 XM_084852
VERSION     NM_001080825.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Hu,R., Cao,Y., Wang,Y., Zhao,T., Yang,K., Fan,M., Guan,M., Hou,Y.,
            Ying,J., Ma,X., Deng,N., Sun,X., Zhang,Y. and Zhang,X.
  TITLE     TMEM120B strengthens breast cancer cell stemness and accelerates
            chemotherapy resistance via beta1-integrin/FAK-TAZ-mTOR signaling
            axis by binding to MYH9
  JOURNAL   Breast Cancer Res 26 (1), 48 (2024)
   PUBMED   38504374
  REMARK    GeneRIF: TMEM120B strengthens breast cancer cell stemness and
            accelerates chemotherapy resistance via beta1-integrin/FAK-TAZ-mTOR
            signaling axis by binding to MYH9.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Ke,M., Yu,Y., Zhao,C., Lai,S., Su,Q., Yuan,W., Yang,L., Deng,D.,
            Wu,K., Zeng,W., Geng,J., Wu,J. and Yan,Z.
  TITLE     Cryo-EM structures of human TMEM120A and TMEM120B
  JOURNAL   Cell Discov 7 (1), 77 (2021)
   PUBMED   34465718
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7510)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7510)
  AUTHORS   Batrakou,D.G., de Las Heras,J.I., Czapiewski,R., Mouras,R. and
            Schirmer,E.C.
  TITLE     TMEM120A and B: Nuclear Envelope Transmembrane Proteins Important
            for Adipocyte Differentiation
  JOURNAL   PLoS One 10 (5), e0127712 (2015)
   PUBMED   26024229
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7510)
  AUTHORS   Shameer,K., Denny,J.C., Ding,K., Jouni,H., Crosslin,D.R., de
            Andrade,M., Chute,C.G., Peissig,P., Pacheco,J.A., Li,R.,
            Bastarache,L., Kho,A.N., Ritchie,M.D., Masys,D.R., Chisholm,R.L.,
            Larson,E.B., McCarty,C.A., Roden,D.M., Jarvik,G.P. and Kullo,I.J.
  TITLE     A genome- and phenome-wide association study to identify genetic
            variants influencing platelet count and volume and their
            pleiotropic effects
  JOURNAL   Hum Genet 133 (1), 95-109 (2014)
   PUBMED   24026423
REFERENCE   6  (bases 1 to 7510)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK126568.1 and AC084018.28.
            
            On Nov 14, 2008 this sequence version replaced NM_001080825.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.391433.1,
                                           SRR1803613.84265.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000449592.7/ ENSP00000404991.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1700              AK126568.1         1-1700
            1701-7510           AC084018.28        119429-125238
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..7510
                     /gene="TMEM120B"
                     /note="transmembrane protein 120B"
                     /db_xref="GeneID:144404"
                     /db_xref="HGNC:HGNC:32008"
                     /db_xref="MIM:616551"
     exon            1..213
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1164
                     /gene="TMEM120B"
                     /codon_start=1
                     /product="transmembrane protein 120B"
                     /protein_id="NP_001074294.2"
                     /db_xref="CCDS:CCDS41852.1"
                     /db_xref="GeneID:144404"
                     /db_xref="HGNC:HGNC:32008"
                     /db_xref="MIM:616551"
                     /translation="MSGQLERCEREWHELEGEFQELQETHRIYKQKLEELAALQTLCS
                     SSISKQKKHLKDLKLTLQRCKRHASREEAELVQQMAANIKERQDVFFDMEAYLPKKNG
                     LYLNLVLGNVNVTLLSNQAKFAYKDEYEKFKLYLTIILLLGAVACRFVLHYRVTDEVF
                     NFLLVWYYCTLTIRESILISNGSRIKGWWVSHHYVSTFLSGVMLTWPNGPIYQKFRNQ
                     FLAFSIFQSCVQFLQYYYQRGCLYRLRALGERNHLDLTVEGFQSWMWRGLTFLLPFLF
                     CGHFWQLYNAVTLFELSSHEECREWQVFVLAFTFLILFLGNFLTTLKVVHAKLQKNRG
                     KTKQP"
     exon            214..332
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            333..449
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            450..509
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            510..605
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            606..695
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            696..761
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            762..823
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            824..916
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            917..981
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1050
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..7510
                     /gene="TMEM120B"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1696
                     /gene="TMEM120B"
                     /note="major polyA site"
     regulatory      3208..3213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM120B"
                     /note="hexamer: AATAAA"
     polyA_site      3230
                     /gene="TMEM120B"
                     /note="major polyA site"
     regulatory      7486..7491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMEM120B"
                     /note="hexamer: AGTAAA"
     polyA_site      7510
                     /gene="TMEM120B"
                     /note="major polyA site"
ORIGIN      
        1 agttgcgcgc gtggctctgg ctgcgcagga acagctggtg cctccgaggg cggtcggcga
       61 gcgcgcgggc gtggggcgct ggggggccgg tcgggcagcg ctgcgggagc agccgccggc
      121 accgccgcct tgcaccatcg catcatgtcc gggcagctgg agcgttgcga gcgcgaatgg
      181 cacgagctgg agggagaatt tcaagaactg caggagacgc acaggatcta caagcagaag
      241 ctggaggagc tggctgcgct gcagacgctg tgtagcagtt ccatcagtaa gcagaagaag
      301 cacctcaagg acttgaagct tacactccag aggtgcaaac gccatgccag tcgggaggag
      361 gcggagctcg ttcagcagat ggcagcgaac atcaaggagc ggcaggacgt cttcttcgac
      421 atggaggcct acctgcccaa gaagaacggg ctctacttga acctggtcct cggcaatgtg
      481 aacgtgaccc tcctcagcaa ccaggccaag ttcgcctaca aggacgaata tgagaagttc
      541 aagctctacc tgaccatcat cctgctcctg ggtgccgtgg catgtcgatt tgtccttcac
      601 tacagggtga ctgacgaagt cttcaacttc ctgctggtgt ggtattactg caccctgacc
      661 attcgggaga gcattctcat cagcaacggc tcaagaatta aaggctggtg ggtgtctcac
      721 cactacgtct ccacattcct gtccggagtg atgctgacct ggcctaatgg acccatttat
      781 cagaagtttc gcaaccagtt cttagcattt tccatttttc agagctgcgt ccagttcctg
      841 caatattatt accagagggg ctgcctctac cggctgcggg ccctggggga gaggaaccac
      901 ctggatctca cagtggaagg gttccagtcc tggatgtggc ggggcctcac ctttctcctg
      961 cccttcctct tctgtggcca tttctggcag ctctacaatg ccgtcacgct gtttgagctc
     1021 tccagccacg aggaatgcag agaatggcag gtgttcgtac tggcgttcac cttcctcatc
     1081 ctcttcctcg gcaacttcct gaccacgctc aaagtcgtgc atgccaagct ccagaagaac
     1141 agaggcaaga caaagcagcc gtgagcctcg ggctcctgtg ccctcggccc ggacttcaga
     1201 ctgcaggggg ctcccgggct ccttcccagc agccctctca ggcccgtggc atcgctggga
     1261 gagggcccag gccctggtcc cccagtggac cccagtggtc tagaggaatg tgagccccgc
     1321 ctgtccgcac agtgtccgcc cacctattta tgacatattt aatgctgggt cccccatcgt
     1381 ccctggaacc cgaggcctca ctcctgtgct tgaaggtggc tgaggccggg ccagtcttcc
     1441 tggggatggg gcctgaagcc tcagggagcc cctctgttcc cactcctgtc atttgaaccc
     1501 ctctgggtgg ggtttggatg tgcctcgcgg ggttggattt atgctgacct gctacttacc
     1561 aggcccaggc tggggtggtg tgaaccctca gtgtcctgtg gcgcccccac cccggggcca
     1621 ctctgcttct gctgtgagcc ccctcctcgc ccaccccgcc acgtggtgag ggttatttta
     1681 agttctcaga gacccaccgc gtctgcctcg tgctcttctt gcccctggag cgctgggggc
     1741 atcctgagtc aggctgtgag aagattcgcc accagagggc gccccgggcc ctgggtcgtc
     1801 caaggggaca aggacgttcc cgtctgtgct tcggggttcc ctgacccccc catcctgcag
     1861 cccaccttcc tgcagtgtcg tggcaggtac ttggagagtg ggaccaagac ccttggcccc
     1921 tgtggagggg aaccacctcc cccctccttc ccagagcttc tggatgttgt gggagggagg
     1981 tgggggtgcc tggcaggctc acccccaggg gccgcaccct gaggcggggt cagagccact
     2041 gggcatcgct tgggtgagat tctgcattcg acgggaggca gagaggccac caggtttggc
     2101 tgagtgctct gccccaccat tcatatatgg gcctcagctc cccctaactg tggccctaca
     2161 aatccctagg ccagacctca cagcagtgcc cacaggcatg ccttgtttag ccctcctcaa
     2221 ggaattttaa aatttttaat tacttttttt ttttttgaga cagggtctgt tgcctaggcg
     2281 ggagtgcagt ggcatcatag gtcactgtag cctccgcctc cagggctcaa gggatcctcc
     2341 cacctcagcc tctggagtag ctgggaccac aatcacacac caccatgccc tgctcctttt
     2401 tttttttttt ttttgagatg ggagtctcga tctgttaccc aggctggagt gcagtgacac
     2461 aatctcagct cactgcaacc tctgcctccc aggttcaagt gattctcctg cctcagcctc
     2521 ccgagtaact gggactacag gcgcgtgcca ccatgcctgg cttttgtttt gttttgtttt
     2581 gttttgtatt tttagtagag acagggtttc accatgtttg ccaggatagt ctcgatctct
     2641 tgaccttgtg atctgcctgc ctcagcctcc caaagtgctg ggattacagg tgtgagccac
     2701 catgcctggt caccctgcta ctttttaata ctttttagta gagatggggt ctcactatgt
     2761 tgcccaggct ggtctccaac tcatgggctc aactcatcct cctatcttgg cctcccaaag
     2821 cgctgggatt acaggtgtga gccactgtgc ctggcctgat tacattttta aaatgagatt
     2881 tccccacccc tacagtgaga ttccacataa aaatccagat ttctgctttc cctagaaaaa
     2941 cggacctggc aacattcccg tggcacagga cgcctactct ccagttgcca caggccctgc
     3001 cacccccctg cccttcattc tggtctcctt tctggtcctt tttaggcacc tgcgtttgcc
     3061 ctagattagg agtcagctag ctgtctgtaa agggccagat ggcaactatt ttgagcttta
     3121 tgggccaggt ggtttgtggc agctactctt cacctgcagc cccagagcac aaggcaatgc
     3181 agaaaggaat gggtgttgct gtgttccaat aaaactttat ttacaaatca ggctgtggcc
     3241 agatgtggcc cacaggctgt agttgttgga cctctaccgt agaccatcat gaggcctggg
     3301 gttcttcgag aacaagaact ccttggttga ttccccaggg tacggcaggg ccttgggaac
     3361 ctgggtgggt tggggagggt ctcagaaggc tccttccttt ggcaacctga cttcttggaa
     3421 gctcaggttt agctgacgcc ccagcactgg gccagggggg ctgaggcagg agatatgggg
     3481 aaggctagga gttgcctgaa gccattatcc catcttaggc gacaccgact cctaggcgcc
     3541 ctccagagcc aagcagcttg cgacttctgg taggcaccgg aatcccctgg gatgcttgtt
     3601 ttaaaagtcc tgggccgggc acggtggctc acgcctgtaa tcccagcact ttgggaggct
     3661 aaggcgggtg gatcacgagg tcaggagttc aagaccagcc tggccaacat ggtgaaaccc
     3721 tgtctctact aaaaatacaa aaattagccg gacgtggtgg tgtgcgcatg tagtcccagc
     3781 tactcaggag gctgaggcaa gagaatcgct tgaacccagg aggtggagct tgcagtgagc
     3841 caagatcatg ccactgcact ctagcctgag tgacagaatg agactctgtc ccaaaaaaaa
     3901 aaaaaaaaaa aaattcccag accctcggcc ccagaccttc ccaaaggagg ctcagggatt
     3961 aggtatctgg ctgttggtga cacaggtggt ctatgaacca gttctgagaa actgtcccgc
     4021 gtggacaggg ggtagatccc atcagttctc aaaggaggcc aggatgtctc gcctcctgaa
     4081 ctccacaggc tggtagaagc ggggcttgag ggacagcagg ttgggagctt gaggactaag
     4141 tgggccagtc ctgtcctacc acagtggggg gaacagtcca cagaaaactt gctcatgacc
     4201 acactgggag ccgactgttc ttcccatccc aagtctctga cactgggggc tttgggtgaa
     4261 accttaagga gatggtcaga tgggtggtcc agggtatact tttaacaact gagaagccat
     4321 tttttctgcc ccctgggcac atgtgcccag ctctgcaaag acagtggcca agttgaagac
     4381 aagtcttcca tgtggggtgg agatgggagg ggtgggtgcc aggctgcagc ctgtgtcctc
     4441 ctgataggcc cagccccctc ggtggcctcc gtgttcctgg gggaaggaag gaaggagctg
     4501 gaggatacag tggtgcctat ctcgggagcc cagggaggca gggacacagg agtggcaggc
     4561 ttggggcgcc cgcgtggaac agtgccaggt ctacgtttac ccactaccag atagactttt
     4621 tcatttcaag cataacttga gtctgcactg gggagacact cgtggtgggg gtggctgtga
     4681 tgagggcact tgcgagtccc gacaacagac actggctcct gcacccacat caccaccatg
     4741 tcccaggggc agcctggagg ggagtttgtg gtcagagccc cagccaggaa aggagagagt
     4801 tccagaatgt tccaagagtc tagccgcagg ccccagacac catgagctgg agggtcggga
     4861 tggggcagcc tccctggtgc aatcggcacc tgggcccccg ggccctgtca gtgctgtcgt
     4921 gaggtctgtc tgccctgggt cagagcagca ggcagagccg gcgcttcttc tgccgttgcg
     4981 ccttcttcag agcgctgaga gccaccttgg cggcctcccg gaagacgtcc tccacattct
     5041 cccgaaactt ggcggaacat tccaggtaga gagcagctcg gatctgttcg caggcgctca
     5101 ggccctgggt ggggggagga gccagcatta ggtgaggggc ccctggaggt ctcctagcac
     5161 cacctggtgg gtttgggact ggctctgagg actctgcagg gatggaggcc ttggtttggg
     5221 cctgtctgtc tcctccatcc tggctgcccc tcacagtgtg tgggtggaat ggagggccag
     5281 ggcagtgcag cccgcaggtt ggaagaagcc ctgtccaggc ccccaccctg gcctctctcc
     5341 agctccgggc agggaggggc tgaatcctga gacccggggt tggttccccc aggtgtctcc
     5401 caggcctgtg agaagagttg gaggcctcaa gacagaaagg acttccagcc acctctctcc
     5461 cttctctgaa agtaccattt aggcaaatta atttgccctt ttatttattt atttttgaga
     5521 tggagttttg ctcttgttac ccaggctgga gtgcaatggc gcgatcttgg ttcatcacaa
     5581 cctctgccac ccaggttcaa gcgattctcc tgcctcagcc tcccgagtag ctgggattac
     5641 aggcatgcgc caccatgccc ggctaatgtt gtatttttag tagagatggg gtttctctca
     5701 tgttggtcag gctggtctca aactcccaac ctcaggtgat tcgcccgcct cggcctccca
     5761 aagtgctggg attacaggcg tgagccacta tgcccggccg gatttgccct tattaatggt
     5821 tatttcttgt gaaagtttct ttttgccttt gcattccttt tattctgttt attcccccat
     5881 gcctcaccca aagagttgca cggtcaattg gcccgtgaag gggacgccta ctttcaaaca
     5941 aggcagacag gacacgacag gacggcggct catagcacag actttggatt gcagtggatg
     6001 ggaccagatc ctggctccac ttctcgctag ctgtgtggcc ctgggcaagc tgcttaacct
     6061 ctctgggcct cagtttctcc ccctgtaaac tgggggatgt gaacagcgcc tgcctccgag
     6121 tcctaaggat tgggagtagt cgtgtaaagt gctcaggtcc acaggccatc aatactaata
     6181 gttaaaaatt attcttagaa tcttgcttcc ctcagctccc tgaaaggcca ctaaggcacc
     6241 ccagttgcag aggccaaagg tccgggaggc ttcacagcca cggctgtgcc ccagggtctt
     6301 ggccccggcc cacctgcatg taggtgatgg gctccagctg ggcggcccgg agcttccgca
     6361 gctgctcctt gtccttcctc aggtctgtct tgcagccgat gagcaccatg gggatcccgc
     6421 ggcagaaatg cgtgacctca gggaaccact gtggagggag gaggcgggat caggggtgcg
     6481 gccgggccca gatgtggggc caccacccag gaggccggcc tcaccttgat gaggacgttg
     6541 tcgtagctgg tgggattcat gacgtcatag cagatgagca cgaggtgggt gttctggtag
     6601 gacaggggcc gcagccggtc atagtcttct tgccctgaaa gcacagagca gcgggggtca
     6661 ggggacgtcc cctccctgtc tggactctga cgggtgaagg ggaaggggcc aggcaagtga
     6721 ccctgcctta gggcctcaat ttcctcatct atacaatggg cagcaagcca ggagtgctgg
     6781 cacaggcctg tggtcgcagc tactcgggag gctgaggccg gaggatcgct tgagcccagg
     6841 aggtcaaggc tacagtgagc cgtgatcatg ccactgcact ccagcctggg tgacagagcg
     6901 agaccctgtc tcttaacaac aaaacccatg agcggcagcc ccccagtcct ggatggtggt
     6961 aaagaatcct caagatcaaa cccacgcagt gctgagagct tggcctgatt ctagggctgg
     7021 ggctggagaa actgctagag atgatgccga tagccagtgt gatccccctg ccctgatggt
     7081 caagggcaga gtgcagactg gaaccctccc ctccccaaag attcagacct gtggggctga
     7141 gtgggctcat agtgtcccca agtcctgaga ggctggtgtc tggcttcagc ctccagcttc
     7201 tcaggttctg atgcagtcag ctgagttccc tgcctattct tgcaagcact aggaggaggg
     7261 tggtgggttg ctgggaacag caccgagcgc cctccccacc cagattcaca gagcacactc
     7321 cctgggggga tactttaatc cggaggccgt gacgcctggc tccgccccga gacgagctga
     7381 atccaaatac ggatctaggc ttgagcttgg ttgggtttgc ttttttcttc ttcttcttta
     7441 taaacaattc tttgtaactt tttgtattga cagtttcaaa cttacagtaa aattgcaaca
     7501 cgagtatgaa
//
