LOCUS       NM_001164468            3512 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens TMED7-TICAM2 readthrough (TMED7-TICAM2), transcript
            variant 1, mRNA.
ACCESSION   NM_001164468
VERSION     NM_001164468.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Palsson-McDermott,E.M., Doyle,S.L., McGettrick,A.F., Hardy,M.,
            Husebye,H., Banahan,K., Gong,M., Golenbock,D., Espevik,T. and
            O'Neill,L.A.
  TITLE     TAG, a splice variant of the adaptor TRAM, negatively regulates the
            adaptor MyD88-independent TLR4 pathway
  JOURNAL   Nat Immunol 10 (6), 579-586 (2009)
   PUBMED   19412184
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010226.5 and AY304584.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001164468.3.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring transmembrane emp24 protein
            transport domain containing 7 (TMED7) and toll-like receptor
            adaptor molecule 2 (TICAM2) genes. Alternative splicing results in
            multiple transcript variants, one of which encodes a fusion protein
            that shares sequence identity with the products of each individual
            gene. This fusion product functions to negatively regulate the
            adaptor MyD88-independent toll-like receptor 4 pathway. [provided
            by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1), also known as TAG.
            This isoform shares sequence identity with the products of both the
            TMED7 and TICAM2 genes.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY304584.1, SRR1660805.205035.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2145313
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 51014, 353376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC010226.5         46816-46837
            23-3357             AY304584.1         1-3335
            3358-3512           AC010226.5         94202-94356
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q22.3"
     gene            1..3512
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /note="TMED7-TICAM2 readthrough"
                     /db_xref="GeneID:100302736"
                     /db_xref="HGNC:HGNC:33945"
     exon            1..436
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..1459
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; TMED7-TICAM2 read-through transcript; TIR
                     domain-containing adapter molecule 2; Putative
                     NF-kappa-B-activating protein 502; Toll-like receptor
                     adaptor protein 3; Toll/interleukin-1 receptor
                     domain-containing protein"
                     /codon_start=1
                     /product="TRAM adaptor with GOLD domain isoform 1
                     precursor"
                     /protein_id="NP_001157940.1"
                     /db_xref="GeneID:100302736"
                     /db_xref="HGNC:HGNC:33945"
                     /translation="MPRPGSAQRWAAVAGRWGCRLLALLLLVPGPGGASEITFELPDN
                     AKQCFYEDIAQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASK
                     NGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKS
                     VIDYQTHFRLREAQGRSRAEDLNTRVAYWHSVDTSPGYHESDSKKSEDLSLCNVAEHS
                     NTTEGPTGKQEGAQSVEEMFEEEAEEEVFLKFVILHAEDDTDEALRVQNLLQDDFGIK
                     PGIIFAEMPCGRQHLQNLDDAVNGSAWTILLLTENFLRDTWCNFQFYTSLMNSVNRQH
                     KYNSVIPMRPLNNPLPRERTPFALQTINALEEESRGFPTQVERIFQESVYKTQQTIWK
                     ETRNMVQRQFIA"
     exon            437..682
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /inference="alignment:Splign:2.1.0"
     exon            683..810
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /inference="alignment:Splign:2.1.0"
     exon            811..3512
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3339..3344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /note="hexamer: AATAAA"
     polyA_site      3359
                     /gene="TMED7-TICAM2"
                     /gene_synonym="MyD88-4; TICAM-2; TICAM2; TIRAP3; TIRP;
                     TRAM"
                     /note="major polyA site"
ORIGIN      
        1 aatccccact tccgaccctc tcaggccttt tccgcttctc ttttacctcc ccaggtccgc
       61 ccgtctgcgc ccctcacagg aagccggagg gtcgctctga tcccgaatct cccacaggcg
      121 tgaacctgct ctgctgtgta tctttgcggg gtggcctgcg ctgaggcctg ccgcgcgcgg
      181 tgagtccgcg cagacctgac cctgcgtctc gcagctcggt tgaggccgcc gccgccttct
      241 cgggatgccg cggccggggt ccgcgcagcg ctgggcggcc gtcgcgggcc gttgggggtg
      301 caggctgctc gcactgctgc tactggtgcc tggacccggc ggcgcctctg agatcacctt
      361 cgagcttcct gacaacgcca agcagtgctt ctacgaggac atcgctcagg gcaccaagtg
      421 caccctggag ttccaggtga ttactggtgg tcactatgat gtagattgtc gattagaaga
      481 tcctgatggt aaagtgttat acaaagagat gaagaaacag tatgatagtt ttaccttcac
      541 agcctccaaa aatgggacat acaaattttg cttcagcaat gaattttcta ctttcacaca
      601 taaaactgta tattttgatt ttcaagttgg agaagaccca cctttgtttc ctagtgagaa
      661 ccgagtcagt gctcttaccc agatggaatc tgcctgtgtt tcaattcacg aagctctgaa
      721 gtctgtcatc gattatcaga ctcatttccg tttaagagaa gctcaaggcc gaagccgagc
      781 agaggatcta aatacaagag tggcctattg gcacagtgtg gatacaagtc caggatatca
      841 tgagtcagat tccaagaagt ctgaagatct atccttgtgt aatgttgctg agcacagcaa
      901 tacaacagag gggccaacag gaaagcagga gggagctcag agcgtggaag agatgtttga
      961 agaagaagct gaagaagagg tgttcctcaa atttgtgata ttgcatgcag aagatgacac
     1021 agatgaagcc ctcagagtcc agaatctgct acaagatgac tttggtatca aacccggaat
     1081 aatctttgct gagatgccat gtggcagaca gcatttacag aatttagatg atgctgtaaa
     1141 tgggtctgca tggacaatct tattactgac tgaaaacttt ttaagagata cttggtgtaa
     1201 tttccagttc tatacgtccc taatgaactc cgttaacagg cagcataaat acaactctgt
     1261 tatacccatg cggcccctga acaatcccct tccccgagaa aggactccct ttgccctcca
     1321 aaccatcaat gccttagagg aagaaagtcg tggatttcct acacaagtag aaagaatttt
     1381 tcaggagtct gtgtataaga cacaacaaac tatatggaaa gagacaagaa atatggtaca
     1441 aagacaattt attgcctgag atgaaacata taacatgtgg ctggctcttg ttttgtaaac
     1501 caaatgatta atcttcactt gagaaagcag tttctaggaa atgtttaaat aaaagagagt
     1561 cttcacctta aagaaaccta tggagcacaa gaaagataaa tttctgcagg acagcctata
     1621 aaattgtggt actttttgat gtttcagtaa acttgacatt gtcagagttt caaggacttt
     1681 tctttcacaa ttttcctagt tcatggatat gaaaaaggaa ttctcaatcc atattccttg
     1741 tattgaacct tgaacaaaaa cttgtatgac agacattttt aaaaatgtga caacactttt
     1801 attctctgaa ttttgatctc aaaggacaca gaaaaaaaat ggccccagga gatctgatca
     1861 cacttcctcc tgaggcacct ctcatggatg ttgcaataag cattcgggta ctatcaccca
     1921 gaaatatgaa ttgccagaat agaacattta gcatgttaag cgttgatgca tataaaatca
     1981 gaaatagatg tgagaatggt ggaacttttt aaaagaaccc agtcaaatgt attttctgct
     2041 gaaatctgca tatttggagg catttcccac caccgattca cagcccattt gatagtgtgg
     2101 tagttaggga cttcgtggag tggtgttcag acgtcccctg gggcttaaat ctcttcatat
     2161 tagtcatcat ttgtaactat ggctttattt gcagagcttc taaaaggcgt ataactgtgt
     2221 gagtggccag atattcactt tttaaatcaa aaacctctct tatggaagct ttaaaagttt
     2281 ccgtcacaca caattctctt ctcaggaagt atttctcatt taggtcttca aagtagcctg
     2341 actgtgtgca tgtgtgtgtg tgataggtta tttataaaga ctttggatag aaggagatgt
     2401 attttattac ctcctattct agagccccat gctcctaaca agccagagag gccccaaaca
     2461 ggattgtttc tttcctccac agcccttctg cccatctgag attgagggag catcgtccac
     2521 ttgagatcag ggatggggtg gagaatgggt catgtcatgt aatgagaaaa gccctcttcg
     2581 ggatcatgag acttggttct agtccaattt ctgccactga ggatgaatgt aactgtgggc
     2641 aaactattta ccctccttta tctgtgaaat gaaagggttg aattgatgga tctctaaagg
     2701 cttttgtcct ctatgaggat gtgaaaaact agggaccaca aaagggaaca agcaaaaaag
     2761 tttggattcg ataaagtgat atgtaatagt tgcagaaggc tttatatatg cttataatga
     2821 aaagatattt tttgtatatt gacagcataa tttattttta atgctgtcat tacacttaaa
     2881 gtcacaggaa aaaaatatac atgcttactc aggctttctt aaaaataaat ttttatagag
     2941 atccttgagt aaagacattt tgcttaattt cttttttctt attccccact tgtatatccc
     3001 ctaccagtac cgggatctgc acacatcttt ttgcagttac ctcttcatag ccatgaacca
     3061 aaacgttcta tgaggagcat gcaagtaagt caagcctcct attctgttag tacttattag
     3121 aggaggagat ggttttcatt gcatagtgac attttcttag ccttaacgtt ctgatagtag
     3181 cttactactc acttctcttt ttcagttttc ataataagta ttcatttttt tgccataatg
     3241 cttcctgtaa agccaatttt atatactaat aaaacatgaa ctgcccactc ttcatgcctg
     3301 ccaaacttgg ggcaattgat gctaaatggt atttttaaaa taaatgtttt tattctttac
     3361 tcttgactaa acttgtttat tttcaactcc aatgaagatt gtggaagagt gttttgaaat
     3421 gaagcatcga tttgtacttc atagagcacc agacacaatg tctcacccaa tttggagttc
     3481 catatttttt ttatgataaa gaaaaatgtt gg
//
