LOCUS       NM_016040               5789 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transmembrane p24 trafficking protein 5 (TMED5),
            transcript variant 1, mRNA.
ACCESSION   NM_016040
VERSION     NM_016040.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Cheng,X., Deng,X., Zeng,H., Zhou,T., Li,D. and Zheng,W.V.
  TITLE     Silencing of TMED5 inhibits proliferation, migration and invasion,
            and enhances apoptosis of hepatocellular carcinoma cells
  JOURNAL   Adv Clin Exp Med 32 (6), 677-688 (2023)
   PUBMED   36530030
  REMARK    GeneRIF: Silencing of TMED5 inhibits proliferation, migration and
            invasion, and enhances apoptosis of hepatocellular carcinoma cells.
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Zou,H., Chen,H., Liu,S. and Gan,X.
  TITLE     Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory
            network in cervical cancer development
  JOURNAL   World J Surg Oncol 19 (1), 246 (2021)
   PUBMED   34404391
  REMARK    GeneRIF: Identification of a novel circ_0018289/miR-183-5p/TMED5
            regulatory network in cervical cancer development.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5789)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5789)
  AUTHORS   Theiler,R., Fujita,M., Nagae,M., Yamaguchi,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     The alpha-helical region in p24gamma2 subunit of p24 protein cargo
            receptor is pivotal for the recognition and transport of
            glycosylphosphatidylinositol-anchored proteins
  JOURNAL   J Biol Chem 289 (24), 16835-16843 (2014)
   PUBMED   24778190
  REMARK    GeneRIF: The luminal alpha-helical but not GOLD domain of p24gamma2
            was required for efficient GPI-AP transport.
REFERENCE   5  (bases 1 to 5789)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
REFERENCE   6  (bases 1 to 5789)
  AUTHORS   Koegler,E., Bonnon,C., Waldmeier,L., Mitrovic,S., Halbeisen,R. and
            Hauri,H.P.
  TITLE     p28, a novel ERGIC/cis Golgi protein, required for Golgi ribbon
            formation
  JOURNAL   Traffic 11 (1), 70-89 (2010)
   PUBMED   19948005
REFERENCE   7  (bases 1 to 5789)
  AUTHORS   Huang,F.X., Zhang,Y. and Wang,W.J.
  TITLE     [Effects of matrine on cgi-100 gene expression and proliferation in
            K562 cells]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 16 (3), 525-530 (2008)
   PUBMED   18549622
  REMARK    GeneRIF: Expression of cgi-100 mRNA decreased in a dose- and
            time-dependent manner in the K562 cells treated with matrine and
            over-expression of cgi-100 elevates the proliferation and the
            immaturity level of K562 cells.
REFERENCE   8  (bases 1 to 5789)
  AUTHORS   Breuza,L., Halbeisen,R., Jeno,P., Otte,S., Barlowe,C., Hong,W. and
            Hauri,H.P.
  TITLE     Proteomics of endoplasmic reticulum-Golgi intermediate compartment
            (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies
            ERGIC-32, a new cycling protein that interacts with human Erv46
  JOURNAL   J Biol Chem 279 (45), 47242-47253 (2004)
   PUBMED   15308636
REFERENCE   9  (bases 1 to 5789)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI436590.1, BC070051.1, AL117354.12 and CA314982.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016040.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.77817.1,
                                           SRR1660807.209501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370282.8/ ENSP00000359305.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AI436590.1         1-51                c
            52-694              BC070051.1         328-970
            695-5093            AL117354.12        11938-16336
            5094-5789           CA314982.1         19-714              c
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.1"
     gene            1..5789
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="transmembrane p24 trafficking protein 5"
                     /db_xref="GeneID:50999"
                     /db_xref="HGNC:HGNC:24251"
                     /db_xref="MIM:616876"
     exon            1..360
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..861
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; transmembrane emp24 domain-containing protein
                     5; p24gamma2; p24 family protein gamma-2; transmembrane
                     emp24 protein transport domain containing 5"
                     /codon_start=1
                     /product="transmembrane emp24 domain-containing protein 5
                     isoform 1 precursor"
                     /protein_id="NP_057124.3"
                     /db_xref="CCDS:CCDS743.1"
                     /db_xref="GeneID:50999"
                     /db_xref="HGNC:HGNC:24251"
                     /db_xref="MIM:616876"
                     /translation="MGDKIWLPFPVLLLAALPPVLLPGAAGFTPSLDSDFTFTLPAGQ
                     KECFYQPMPLKASLEIEYQVLDGAGLDIDFHLASPEGKTLVFEQRKSDGVHTVETEVG
                     DYMFCFDNTFSTISEKVIFFELILDNMGEQAQEQEDWKKYITGTDILDMKLEDILESI
                     NSIKSRLSKSGHIQTLLRAFEARDRNIQESNFDRVNFWSMVNLVVMVVVSAIQVYMLK
                     SLFEDKRKSRT"
     exon            361..458
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /inference="alignment:Splign:2.1.0"
     exon            459..642
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /inference="alignment:Splign:2.1.0"
     exon            643..5789
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1160..1165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAA"
     polyA_site      1187
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="major polyA site"
     regulatory      1314..1319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAA"
     polyA_site      1335
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="major polyA site"
     regulatory      3626..3631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAG"
     regulatory      3661..3666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAA"
     polyA_site      3668
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
     polyA_site      3681
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="major polyA site"
     regulatory      5347..5352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAA"
     polyA_site      5372
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
     regulatory      5769..5774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
                     /note="hexamer: AATAAA"
     polyA_site      5789
                     /gene="TMED5"
                     /gene_synonym="CGI-100; p24g2; p28"
ORIGIN      
        1 ggagaactgc gcttctcttt cggagggagt gttcgccgcc gccgcggccg ccacctggag
       61 tttcttcaga ctccagattt ccctgtcaac cacgaggagt ccagagagga aacgcggagc
      121 ggagacaaca gtacctgacg cctctttcag cccgggatcg ccccagcagg gatgggcgac
      181 aagatctggc tgcccttccc cgtgctcctt ctggccgctc tgcctccggt gctgctgcct
      241 ggggcggccg gcttcacacc ttccctcgat agcgacttca cctttaccct tcccgccggc
      301 cagaaggagt gcttctacca gcccatgccc ctgaaggcct cgctggagat cgagtaccaa
      361 gttttagatg gagcaggatt agatattgat ttccatcttg cctctccaga aggcaaaacc
      421 ttagtttttg aacaaagaaa atcagatgga gttcacactg tagagactga agttggtgat
      481 tacatgttct gctttgacaa tacattcagc accatttctg agaaggtgat tttctttgaa
      541 ttaatcctgg ataatatggg agaacaggca caagaacaag aagattggaa gaaatatatt
      601 actggcacag atatattgga tatgaaactg gaagacatcc tggaatccat caacagcatc
      661 aagtccagac taagcaaaag tgggcacata caaactctgc ttagagcatt tgaagctcgt
      721 gatcgaaaca tacaagaaag caactttgat agagtcaatt tctggtctat ggttaattta
      781 gtggtcatgg tggtggtgtc agccattcaa gtttatatgc tgaagagtct gtttgaagat
      841 aagaggaaaa gtagaactta aaactccaaa ctagagtacg taacattgaa aaatgaggca
      901 taaaaatgca ataaactgtt acagtcaaga ccattaatgg tcttctccaa aatattttga
      961 gatataaaag taggaaacag gtataatttt aatgtgaaaa ttaagtcttc actttctgtg
     1021 caagtaatcc tgctgatcca gttgtactta agtgtgtaac aggaatattt tgcagaatat
     1081 aggtttaact gaatgaagcc atattaataa ctgcattttc ctaactttga aaaattttgc
     1141 aaatgtctta ggtgatttaa ataaatgagt attgggccta attgcaacac cagtctgttt
     1201 ttaacaggtt ctattaccca gaactttttt gtaaatgcgg cagttacaaa ttaactgtgg
     1261 aagttttcag ttttaagtta taaatcacct gagaattacc taatgatgga ttgaataaat
     1321 ctttagacta caaaagccca acttttctct atttacatat gcatctctcc tataatgtaa
     1381 atagaataat agctttgaaa tacaattagg tttttgagat ttttataacc aaatacattt
     1441 cagtgtaaca tattagcaga aagcattagt ctttgtactt tgcttacatt cccaaaagct
     1501 gacattttca cgattcttaa aaacacaaag ttacacttac taaaattagg acatgttttc
     1561 tctttgaaat gaagaatata gtttaaaagc ttcctcctcc atagggacac attttctcta
     1621 acccttaact aaagtgtagg attttaaaat taaatgtgag gtaaaataag tttattttta
     1681 atagtatctg tcaagttaat atctgtcaac agttaataat catgttatgt taattttaac
     1741 atgattgctg acttggataa ttcattatta ccagcagtta tgaaggaaat attgctaaaa
     1801 tgatctgggc ctaccataaa taaatatctc cttttctgag ctctaagaat tatcagaaac
     1861 aggaaagaat ttagaaaaac ttgagaaaac ctaatccaaa ataaaattca cttaagtaga
     1921 actataaata aatatctaga atctgactgg ctcatcatga catcctactc ataacataaa
     1981 tcaaaggaga tgattaattt ccagttagct ggaagaaact ttggctgtag gtttttattt
     2041 tctacaagaa ttctggtttg aattattttt gtaagcaggt acattttata aaatgtaagc
     2101 cctactgtaa ggtttagcac tgggtgtaca tatttattaa aaatttttat tataacaact
     2161 tttattaaaa tggcctttct gaacacttta tttattgatg ttgaagtaag gattagaaac
     2221 atagactccc aagttttaaa cacctaaatg tgaataaccc atatatacaa caaagtttct
     2281 gccatctagc tttttgaagt ctatgggggt cttactcaag tactagtaat ttaacttcat
     2341 catgaatgaa ctataatttt taagttatgc ccatttataa cgttgtttat gactacattg
     2401 tgagttagaa acaaacttaa aatttggggt atagaacccc tcaacaggtt agtaatgctg
     2461 gaattcttga tgagcaataa tgataaccag agagtgattt catttacact catagtagta
     2521 taaaaagaga tacatttccc tcttaggccc ctgggagaag agcagcttag atttccctac
     2581 tggcaaggtt tttaaaaatg aggtaaatgc cgtatatgat caattacctt aattggccaa
     2641 gaaaatgctt caggtgtcta ggggtatcct ctgcaacact tgcagaacaa aggtcaataa
     2701 gatccttgcc tatgaatacc cctccctttt gcgctgttaa atttgcaatg agaagcaaat
     2761 ttacagtacc ataactaata aagcagggta cagatataaa ctactgcatc ttttctataa
     2821 aactgtgatt aagaattcta cctctcctgt atggctgtta ctgtactgta ctctctgact
     2881 ccttacctaa caatgaattt gttacataat cttctacatg tatgatttgt gccactgatc
     2941 ttaaacctat gattcagtaa cttcttacca tataaaaacg ataattgctt tatttggaaa
     3001 agaatttagg aatactaagg acaattattt ttatagacaa agtaaaaaga cagatattta
     3061 agaggcataa ccaaaaaagc aaaacttgta aacagagtaa aaatctttaa tatttctaaa
     3121 gacatactgt ttatctgctt catatgcttt ttttaatttc actattccat ttctaaatta
     3181 aagttatgct aaattgagta agctgtttat cacttaacag ctcattttgt ctttttcaat
     3241 atacaaattt taaaaatact acaatattta actaaggccc aaccgatttc cataatgtag
     3301 cagttaccgt gttcacctca cactaaggcc tagagtttgc tctgatatgc atttggatga
     3361 ttaatgttat gctgttcttt catgtgaatg tcaagacatg gagggtgttt gtaattttat
     3421 ggtaaaatta atccttctta cacataatgg tgtcttaaaa ttgacaaaaa atgagcactt
     3481 acaattgtat gtctcctcaa atgaaaattc tttatctgaa attttaaaag acattgattc
     3541 cacatgtaag gatttttcat ctgaagtaca ataatgcaca atcagtgttg ctcaaactgc
     3601 tttatactta taaacagcca tcttaaataa gcaacgtatt gtgagtactg atatgtatat
     3661 aataaaaatt atcaaagaaa attaactgcc agtcatttgt aaatgctgtc tactcctgaa
     3721 agccttcctt catctctatt tagttaataa tgtgctctct cctcctgttt tgaaacccca
     3781 tagctctcta cacctcttat tgtacatcac tctactttgc attggagttc tgtttctata
     3841 tgtacttatt cacctgttgc ctaaacatgc actgttccta aaaggtacag tttttgctca
     3901 aaatatatga aaggaaagat aatcagttct taaagtttgg tatgctaaat aagcacccag
     3961 ggaacttacc tgctccacct caccccagaa attgattatt caggtctgga atatggctca
     4021 ggattcttaa ttttcaacca acacaatata gaatcctgtc agttatcctt ggatcatttg
     4081 ctaagtgatc ttgcaaaatc aaaactcctt tgagactatt tcattttcag aaatatttta
     4141 aaacttgttc agaaacatct taaaacttgt tagcatatag gaaaagtaca atgcttggtg
     4201 taggtaggtt attatttggt catttccctc aaaccatcca gacaagccac tcttctacca
     4261 caagctggta gaaatcctta ggcacctttc tgcttctgca ctgctatcac tttcaggtgt
     4321 ctaaggcttc tggaaggctg ttgtcataac ctggagccat acacctaatg tacgtctggg
     4381 gaaaggacac caattaaaga atctagagct aggatgatgt tacataagac agggcacaat
     4441 ggagatggac tctggatccc tgcatctgaa agaaaaaggc aagaaaatgc agggcaggga
     4501 aaatcctgaa ggtcttcact gaccatgaat taaaatccta attgacagtt ttacaaacaa
     4561 aaaccctcgt tttagtactt gtgttgtttt tgtttctgtt ttaacaagct tagatttatt
     4621 attgtgcctt ttatggaaga tgaggaagaa ataggaaagc agtaaaatga acaatgagaa
     4681 tctgttaccc aaactgatat aaactccctc agaaagaaag atactactcc ctaatcctgg
     4741 aagtgttaag tggcaataat gatcaaatgt tgtaaagggg attgttacta cagctaatat
     4801 gaaatgggag gctggactaa atctaacctt tctacccttg atttcaattc taaaaggaca
     4861 cgtaatacca ttgcagaaag aaaagacagt tccaaatgat aaattttaga gttgttttgc
     4921 taggatgcaa gagaaattgg ataagtggac cactcatacg ttgctggtga gatatgaaat
     4981 agtagagcca ctgtggaaaa tattcttggc agtttcttat aaaactaaac atacatttaa
     5041 catataactg gcatttgtac tacttgacag ttatccctga gaagttaaaa cttaacattc
     5101 acaaatgtta catagcacct tcatttataa tagccaaaac ccggaaacaa cctggatgtc
     5161 cttcaaaaag agtgaatggt ttagtatgtc cacaccatgg aatactaaat agtaatatga
     5221 aggaacaaac tactgataca tgtaacaaat tgcatggacc tcaagggaat tatgcttggg
     5281 tggtttgggg ggacaatcct aaagttttac acactgtatg attctgtttt tgtaacattc
     5341 ttaaataata aagctataga gatgggaaac aagattcgtg gttgccaggt cttgggactg
     5401 aagggaagga gaatgtggct ataaagggag ccttgtggtg gaaaagttct gtagtttgat
     5461 tgtggtgatt acataaatat acatgtgaaa tgttagaagt atttatagct gaaacatatt
     5521 agtatgttgg tatatacata tttctgacta attaatttcg ccatgttgcc caggctggtc
     5581 tcgaactcct aagctcaagt gatgtgcccg cctcaacttc ccaagtgctg agattacagg
     5641 cgtgagccac catgtccggc ctgttttttc ctatgcatgc ataattacga tcaagtttaa
     5701 tttgtaatta ggcacattaa gagattaaca atagctaaca aaatggaaca attacaacaa
     5761 tatattgtaa taaaagttat gcaaatgtg
//
