LOCUS       NM_182547               2294 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transmembrane p24 trafficking protein 4 (TMED4),
            transcript variant 1, mRNA.
ACCESSION   NM_182547
VERSION     NM_182547.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Hwang,S.O., Boswell,S.A., Seo,J.S. and Lee,S.W.
  TITLE     Novel oxidative stress-responsive gene ERS25 functions as a
            regulator of the heat-shock and cell death response
  JOURNAL   J Biol Chem 283 (19), 13063-13069 (2008)
   PUBMED   18326488
  REMARK    GeneRIF: ERS25 may play a critical role in regulation of heat-shock
            response and apoptosis
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Tsuritani,K., Irie,T., Yamashita,R., Sakakibara,Y., Wakaguri,H.,
            Kanai,A., Mizushima-Sugano,J., Sugano,S., Nakai,K. and Suzuki,Y.
  TITLE     Distinct class of putative 'non-conserved' promoters in humans:
            comparative studies of alternative promoters of human and mouse
            genes
  JOURNAL   Genome Res 17 (7), 1005-1014 (2007)
   PUBMED   17567985
REFERENCE   3  (bases 1 to 2294)
  AUTHORS   Matsuda,A., Suzuki,Y., Honda,G., Muramatsu,S., Matsuzaki,O.,
            Nagano,Y., Doi,T., Shimotohno,K., Harada,T., Nishida,E., Hayashi,H.
            and Sugano,S.
  TITLE     Large-scale identification and characterization of human genes that
            activate NF-kappaB and MAPK signaling pathways
  JOURNAL   Oncogene 22 (21), 3307-3318 (2003)
   PUBMED   12761501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA419871.1, BC052641.1, BX647965.1, AC004938.3 and BC035467.1.
            
            On Nov 22, 2018 this sequence version replaced NM_182547.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.838735.1,
                                           SRR3476690.923769.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154405
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000457408.7/ ENSP00000404042.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-415               DA419871.1         65-479
            416-778             BC052641.1         413-775
            779-1324            BX647965.1         759-1304
            1325-1339           AC004938.3         10341-10355
            1340-2294           BC035467.1         1328-2282
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..2294
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="transmembrane p24 trafficking protein 4"
                     /db_xref="GeneID:222068"
                     /db_xref="HGNC:HGNC:22301"
                     /db_xref="MIM:612038"
     exon            1..185
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..709
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; endoplasmic reticulum stress-response protein
                     25 kDa; putative NFkB activating protein HNLF;
                     transmembrane emp24 domain-containing protein 4; putative
                     NF-kappa-B-activating protein 156; p24 family protein
                     alpha-3; transmembrane emp24 protein transport domain
                     containing 4"
                     /codon_start=1
                     /product="transmembrane emp24 domain-containing protein 4
                     isoform 1 precursor"
                     /protein_id="NP_872353.2"
                     /db_xref="CCDS:CCDS5493.1"
                     /db_xref="GeneID:222068"
                     /db_xref="HGNC:HGNC:22301"
                     /db_xref="MIM:612038"
                     /translation="MAGVGAGPLRAMGRQALLLLALCATGAQGLYFHIGETEKRCFIE
                     EIPDETMVIGNYRTQMWDKQKEVFLPSTPGLGMHVEVKDPDGKVVLSRQYGSEGRFTF
                     TSHTPGDHQICLHSNSTRMALFAGGKLRVHLDIQVGEHANNYPEIAAKDKLTELQLRA
                     RQLLDQVEQIQKEQDYQRYREERFRLTSESTNQRVLWWSIAQTVILILTGIWQMRHLK
                     SFFEAKKLV"
     exon            186..286
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /inference="alignment:Splign:2.1.0"
     exon            287..412
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /inference="alignment:Splign:2.1.0"
     exon            413..559
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /inference="alignment:Splign:2.1.0"
     exon            560..2294
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1860..1865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="hexamer: AATAAA"
     polyA_site      1878
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="major polyA site"
     regulatory      2267..2272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="hexamer: ATTAAA"
     polyA_site      2294
                     /gene="TMED4"
                     /gene_synonym="ERS25; GMP25iso; HNLF; p24a3; p24alpha3"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgctt aggggctgag gcgcgatggc aggtgtcggg gctgggcctc tgcgggcgat
       61 ggggcggcag gccctgctgc ttctcgcgct gtgcgccaca ggcgcccagg ggctctactt
      121 ccacatcggc gagaccgaga agcgctgttt catcgaggaa atccccgacg agaccatggt
      181 catcggcaac tatcgtaccc agatgtggga taagcagaag gaggtcttcc tgccctcgac
      241 ccctggcctg ggcatgcacg tggaagtgaa ggaccccgac ggcaaggtgg tgctgtcccg
      301 gcagtacggc tcggagggcc gcttcacgtt cacctcccac acgcccggtg accatcaaat
      361 ctgtctgcac tccaattcta ccaggatggc tctcttcgct ggtggcaaac tgcgggtgca
      421 tctcgacatc caggttgggg agcatgccaa caactaccct gagattgctg caaaagataa
      481 gctgacggag ctacagctcc gcgcccgcca gttgcttgat caggtggaac agattcagaa
      541 ggagcaggat taccaaaggt atcgtgaaga gcgcttccga ctgacgagcg agagcaccaa
      601 ccagagggtc ctatggtggt ccattgctca gactgtcatc ctcatcctca ctggcatctg
      661 gcagatgcgt cacctcaaga gcttctttga ggccaagaag ctggtgtagt gccctctttg
      721 tatgaccctt cctttttacc tcatttattt ggtactttcc ccacacagtc ctttatccac
      781 ctggattttt agggaaaaaa atgaaaaaga ataagtcaca ttggttccat ggccacaaac
      841 cattcagatc agccacttgc tgaccctggt tcttaaggac acatgacatt agtccaatct
      901 ttcaaaatct tgtcttaggg cttgtgagga atcagaacta acccaggact cagtcctgct
      961 tcttttgcct cgagtgattt tcctctgttt ttcactaaat aagcaaatga aaactctctc
     1021 cattaccttc tgctttctct ttgtccactt acgcagtagg tgactggcat gtgccacaga
     1081 gcaggccctg cctcactgtc tgctggtcag ttctgggttc acttaatggc tttgtgaatg
     1141 taaataaggg gcaggtcttg gccctagagg attgagatgt ttttctaaat cttagaacta
     1201 tttttggata aattatatat tttccttcct agtagaagtg ttactgcctg taactagctc
     1261 aaaataccaa tgcagtttct gcattctggg ttttgttttt cctttttttt tttttttttt
     1321 ttttttgagt tttgctcttg tcgcccaggc tggagtgcaa tggcgtgatc tcagctcact
     1381 ggcaacatct gcctcccggg ttcaaatgat tctcctgcct cagtctcctg agtagctggg
     1441 attacaggtg cccgccacca cgctcagcta atttttgtat ttttagtaga gatggggttt
     1501 taccatgttg gccaggctgg tcttagactc ctgacctcag ttgatccacc tgcctcagcc
     1561 tctgcattca gtttattcac atatttttgg taactcccat ggcagctcct aggatttcag
     1621 cggtctgtgg gccagaaagc aggcaccagg gctgacctca aggccgtatc agagggccaa
     1681 gcagagttct tttggatacc tgcttttcat cccacagggc cttagagtca gaggtaaggt
     1741 agcaacagag ctagaatggg gcaatgcact cttaccctcc ttctcaactt ttatttaagc
     1801 tgtgctaaat gttttcttca agggaaccag atttagttct ttacagaatt ttccagtgaa
     1861 ataaaacatg ttgtaatagc tgtgtttgag atgaaataag aggttgtggg tagaggggag
     1921 gcacctaaag gaaaagagga aaggtgcctg ggctacctat gcagataacc tggagtggac
     1981 ttcactgtgg actcgtggta ctaaggcttg gcctggacag gcagtctagg gggtatggga
     2041 atacacggtg tggttgttca actatttgca aaggtcaacc aaatagacca catgttcgca
     2101 aagtatcatc tgaggaaatt aagtaccttc ttagccctct cagtcataaa tttgaacaaa
     2161 ttttaataca cttccctcat gcccttctat ataaaactta ataccattag ttccccattc
     2221 ttgacatttt atttcagttt ttattatata tttatttgaa atatttatta aattatctga
     2281 cctacagaac taaa
//
