LOCUS       NM_012290               5723 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens tousled like kinase 1 (TLK1), transcript variant 1,
            mRNA.
ACCESSION   NM_012290
VERSION     NM_012290.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Lin,H.Y., Mohammadhosseini,M., McClatchy,J., Villamor-Paya,M.,
            Jeng,S., Bottomly,D., Tsai,C.F., Posso,C., Jacobson,J., Adey,A.,
            Gosline,S., Liu,T., McWeeney,S., Stracker,T.H. and Agarwal,A.
  TITLE     The TLK-ASF1 histone chaperone pathway plays a critical role in
            IL-1beta-mediated AML progression
  JOURNAL   Blood 143 (26), 2749-2762 (2024)
   PUBMED   38498025
  REMARK    GeneRIF: The TLK-ASF1 histone chaperone pathway plays a critical
            role in IL-1beta-mediated AML progression.
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Kuang,L., Lu,A. and Yao,S.
  TITLE     CircTLK1 alleviates oxygen-glucose deprivation/reperfusion induced
            apoptosis in HK-2 cells through miR-136-5p/Bcl2 signal axis
  JOURNAL   Ren Fail 45 (1), 2236219 (2023)
   PUBMED   37462140
  REMARK    GeneRIF: CircTLK1 alleviates oxygen-glucose deprivation/reperfusion
            induced apoptosis in HK-2 cells through miR-136-5p/Bcl2 signal
            axis.
REFERENCE   3  (bases 1 to 5723)
  AUTHORS   Zhang,Z. and Liu,S.
  TITLE     The interaction between ASF1B and TLK1 promotes the malignant
            progression of low-grade glioma
  JOURNAL   Ann Med 55 (1), 1111-1122 (2023)
   PUBMED   36947060
  REMARK    GeneRIF: The interaction between ASF1B and TLK1 promotes the
            malignant progression of low-grade glioma.
REFERENCE   4  (bases 1 to 5723)
  AUTHORS   Ghosh,I. and De Benedetti,A.
  TITLE     Untousling the Role of Tousled-like Kinase 1 in DNA Damage Repair
  JOURNAL   Int J Mol Sci 24 (17), 13369 (2023)
   PUBMED   37686173
  REMARK    GeneRIF: Untousling the Role of Tousled-like Kinase 1 in DNA Damage
            Repair.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5723)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 5723)
  AUTHORS   Li,Y., DeFatta,R., Anthony,C., Sunavala,G. and De Benedetti,A.
  TITLE     A translationally regulated Tousled kinase phosphorylates histone
            H3 and confers radioresistance when overexpressed
  JOURNAL   Oncogene 20 (6), 726-738 (2001)
   PUBMED   11314006
REFERENCE   7  (bases 1 to 5723)
  AUTHORS   Cabaniols,J.P., Ravichandran,V. and Roche,P.A.
  TITLE     Phosphorylation of SNAP-23 by the novel kinase SNAK regulates
            t-SNARE complex assembly
  JOURNAL   Mol Biol Cell 10 (12), 4033-4041 (1999)
   PUBMED   10588641
REFERENCE   8  (bases 1 to 5723)
  AUTHORS   Sillje,H.H., Takahashi,K., Tanaka,K., Van Houwe,G. and Nigg,E.A.
  TITLE     Mammalian homologues of the plant Tousled gene code for
            cell-cycle-regulated kinases with maximal activities linked to
            ongoing DNA replication
  JOURNAL   EMBO J 18 (20), 5691-5702 (1999)
   PUBMED   10523312
REFERENCE   9  (bases 1 to 5723)
  AUTHORS   Yamakawa,A., Kameoka,Y., Hashimoto,K., Yoshitake,Y., Nishikawa,K.,
            Tanihara,K. and Date,T.
  TITLE     cDNA cloning and chromosomal mapping of genes encoding novel
            protein kinases termed PKU-alpha and PKU-beta, which have nuclear
            localization signal
  JOURNAL   Gene 202 (1-2), 193-201 (1997)
   PUBMED   9427565
REFERENCE   10 (bases 1 to 5723)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009953.4, BC032657.1,
            AC010092.4 and DN604386.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012290.4.
            
            Summary: The protein encoded by this gene is a serine/threonine
            kinase that may be involved in the regulation of chromatin
            assembly. The encoded protein is only active when it is
            phosphorylated, and this phosphorylation is cell cycle-dependent,
            with the maximal activity of this protein coming during S phase.
            The catalytic activity of this protein is diminished by DNA damage
            and by blockage of DNA replication. Three transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D50927.2, BC032657.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000431350.7/ ENSP00000411099.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-443               AC009953.4         29145-29587
            444-1364            BC032657.1         12-932
            1365-1365           AC009953.4         140144-140144
            1366-2876           BC032657.1         934-2444
            2877-2877           AC010092.4         35759-35759
            2878-4488           BC032657.1         2445-4055
            4489-5624           AC010092.4         37371-38506
            5625-5723           DN604386.1         7-105
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..5723
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="tousled like kinase 1"
                     /db_xref="GeneID:9874"
                     /db_xref="HGNC:HGNC:11841"
                     /db_xref="MIM:608438"
     exon            1..604
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             466..2766
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine threonine protein kinase; serine/threonine-protein
                     kinase tousled-like 1; SNARE protein kinase SNAK"
                     /codon_start=1
                     /product="serine/threonine-protein kinase tousled-like 1
                     isoform 1"
                     /protein_id="NP_036422.3"
                     /db_xref="CCDS:CCDS2241.1"
                     /db_xref="GeneID:9874"
                     /db_xref="HGNC:HGNC:11841"
                     /db_xref="MIM:608438"
                     /translation="MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRP
                     REGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLG
                     SLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSP
                     VRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTM
                     LKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKK
                     LLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQ
                     QREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLT
                     LAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHP
                     TLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACRE
                     YRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVM
                     QIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGM
                     DLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQD
                     ILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRR
                     SNSSGNLHMAGLTASPTPPSSSIITY"
     exon            605..723
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            724..795
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            796..871
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            872..918
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1014
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1104
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1197
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1308
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1445
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1634
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1701
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1799
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1881
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1973
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2063
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2233
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2369
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2481
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2589
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..5723
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4355..4360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: AATAAA"
     polyA_site      4381
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="major polyA site"
     regulatory      4464..4469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: AATAAA"
     polyA_site      4488
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="major polyA site"
     regulatory      4634..4639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: ATTAAA"
     polyA_site      4664
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
     regulatory      4758..4763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: CATAAA"
     polyA_site      4789
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
     regulatory      5295..5300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: ATTAAA"
     polyA_site      5319
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
     regulatory      5699..5704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
                     /note="hexamer: ATTAAA"
     polyA_site      5723
                     /gene="TLK1"
                     /gene_synonym="PKU-beta"
ORIGIN      
        1 gcagaggtta ccccaccgcc tcagccgcca cccacagccc gccgccgccg cctcccgggc
       61 gacgccgggg gccgacgcac tgcagaggtg ccggcaggtt ccgcccgcgg aagtgtcctc
      121 ggccggcgca gcccgcgctc cgcccgcgct ccgaccccgg cccggctggc accccctcga
      181 cgcctccgcc gccgcgctcc tttcctcctc ctctcctctg gcgcgcccgc tcgctccctc
      241 gcttctcccc ctttccctcc tccctgtccc ctctccttcc ctcacctctt tcctccccac
      301 ctcccctctt ctcggtttcc tcccccatcc ccttgactct cccctcccag ccctcgctct
      361 ctcgctcgcc ctcagcgcgg cccccgccat gacggaggcg ggtgccggtg ccgttgccgc
      421 cgctgccgtc gcaggggggg agtcgggttc ccagaaagta gcttgatgag tgtccaaagt
      481 agcagtggaa gtttggaggg gccgccatct tggtcccagc tctccacgtc tccaaccccg
      541 ggctcggcgg cggcggccag gtccctgctg aatcacacgc cgccatccgg gaggcccagg
      601 gaaggtgcaa tggatgagct tcatagtctg gatccaagaa ggcaagagtt attggaagct
      661 agatttactg gagttgcaag tgggagcact ggaagtacgg gcagttgcag tgttggagct
      721 aaagcctcaa caaataacga aagctctaat cacagttttg gaagcttggg atctttaagt
      781 gacaaagaat cagagacacc ggagaagaaa caatcggaat catccagggg aagaaagaga
      841 aaagcagaaa accagaatga aagtagtcag ggaaaaagta ttgggggacg tggccacaaa
      901 attagcgact attttgaata ccagggtgga aatggctcaa gtccagtaag aggcatacct
      961 cctgcaatcc gttctcctca aaattcacat tcacattcca ctccttcctc atctgttcga
     1021 ccgaatagcc cttctcctac tgcattagca tttggggacc accctattgt acaaccaaag
     1081 caattatcct ttaaaattat tcagactgat ctcacaatgc tgaaattagc agcattagaa
     1141 agtaataaaa tccaggacct ggaaaagaag gagggacgta tagatgattt gctcagggct
     1201 aactgtgatc tcagacggca aatagatgaa caacaaaaat tacttgaaaa atacaaagaa
     1261 cgattaaata agtgcatatc aatgagcaag aaacttctta ttgaaaagag tacacaagaa
     1321 aagctgtcaa gcagagagaa gagtatgcaa gatcgattac gcctcgggca ctttacaaca
     1381 gttagacatg gcgcttcatt tactgaacaa tggacagatg gttttgcatt tcagaatctt
     1441 gtgaagcaac aagaatgggt gaatcagcaa agggaagata ttgaaaggca aaggaaactt
     1501 ctagccaaac gcaaacctcc cacagctaat aattctcagg caccctctac caattctgaa
     1561 ccaaaacaaa ggaaaaacaa agcagtcaat ggagcagaga atgatccctt tgttagacca
     1621 aatttaccac aactgttgac tttggcagaa tatcatgaac aggaagaaat tttcaaactt
     1681 agactaggac atctcaaaaa ggaagaggca gaaatccagg cagaacttga acgtttggaa
     1741 agagtcagaa atcttcacat acgtgagctg aaaagaataa acaatgaaga taattcacag
     1801 ttcaaagatc acccaacatt aaatgaaaga tatttattac ttcatctgct tggtagaggt
     1861 ggctttagtg aagtgtataa ggcttttgac ctttatgaac aaagatatgc tgctgtgaag
     1921 atacatcagc ttaataaaag ctggagagat gagaagaaag aaaactacca caaacatgcc
     1981 tgcagagagt atagaataca caaagaactg gatcacccca gaatagttaa actctatgat
     2041 tatttctcct tggatacaga tacgttttgt acagtgttag aatactgtga aggcaatgac
     2101 ttggatttct atctgaagca acacaagtta atgtcagaga aagaagctcg gtctattgta
     2161 atgcagattg taaatgcact aagatatctc aatgagatca aaccccctat tatacattat
     2221 gatcttaagc caggaaacat cctactggta gatggaacag catgtggtga aatcaaaatc
     2281 actgattttg gtctgtccaa gattatggat gatgatagct atggtgtaga tggaatggat
     2341 ctaacttccc agggggcagg cacttactgg tatttacctc ctgagtgttt tgtagttgga
     2401 aaagagccac caaagatttc caacaaggtt gatgtatggt cggttggagt catcttcttt
     2461 cagtgtcttt atggtagaaa gccatttggt cacaatcaat ctcaacaaga cattcttcaa
     2521 gaaaatacaa tattaaaagc cacagaagtc cagttccctg taaaaccggt tgtaagcagt
     2581 gaagccaagg catttataag acgctgtttg gcatatcgaa aagaagatcg atttgatgtg
     2641 caccagctgg caaatgaccc ataccttctc ccacacatga gaagatcaaa ttcttcagga
     2701 aacctacaca tggctgggct gacagcatcc cctacacccc cttcttcaag cataattact
     2761 tactgacttt cctccaagat tggcatgata tctttgaatt tgcttccaga tgcacactta
     2821 agtttgagag catttgagtg tttgttttct ttttcttttt tttttttttt tttttttaca
     2881 caagacgtgg ttaagaactg tttgtgaact gaagttcctc atagtgtcat ttgtatgaga
     2941 gaggatcatg gacaatgaat aaatgcacac ttctgactat aattttgagc agtgaaggaa
     3001 ggatgactgc ctgttacaca gaaacaggaa taccatgtta agatagagga aaaaaatttt
     3061 gtttatttgg gggaacactg taaataacgt ttataatact taaatacatt tggttgttac
     3121 tagagagttc taatatgaag ggtagttttt cattatttaa aaacacatgg aaaaatatga
     3181 gacatatttc taacatacga actatagtgc ctggatacat tcttcagcat ttggcagtta
     3241 atctgctgaa accaaagtaa gaactgaatg acagtgacag ttgtgtttta tagtaataga
     3301 atgtgagagt tagaatcttt ggacaaagtt aggctttgtc atttgcctat tcagactttt
     3361 accaagttgt acctcgagat cacatgtccg ttttctttca ctggttatat taaagggaga
     3421 gctattattt ctaaatactt tacacctttg acaaaatgag ctgcttgtgt tgaaatcagt
     3481 tgaattcact aaattataat attgccagtg ttttcaacac gttagaattg ataacaggta
     3541 tttttctttt gttaccaggc cttgttcatc aaaacaggtg acagtgagta accaaatgca
     3601 gaccagttct atgcaggata atgataaatt cccttctagc tctattgtaa attgttgtac
     3661 agatgtcata tttcaattac taagtttcag cagcttattc ttgtacaaat gtttaaaata
     3721 tggcttttct aattggatat tgtatttttt ttaagtcttc ttgaaggcac tctaattgct
     3781 gctaaatgat gttgcttctt tgctaaaaaa ttaaatgacc tagaaggtgc aagttgactg
     3841 tacatcatag atattaaaaa gcagacagtc attaacaatc aagatgtaaa agtgatccat
     3901 gttggacata attgagtttt taaatcagtt tattgggttc tagggctgta gaacacatag
     3961 atactagatt tttaatttgt tcatggttat tctacatttc tagaaagttc ttatcagcaa
     4021 gatggtccca taagcaaatt tcttgtggtt tgtacagatt tgttaaaatg tgaacgtttt
     4081 ctaactgcct cgtagggtag aaaccaatat ttttcaggat gctgtatttc actcctgtaa
     4141 ggactttttc tttattgtca cattcataat gctgatactt acatgtaagt tgttcatgtt
     4201 gcagaaaaag gttatacgta ccacggattc tctttaatat tgtacagtta aactgtattt
     4261 ctgatgttgc aagccatttt gttttttcat tgtacataga cacattgtct ctttaaaatg
     4321 ctgctgaaat tgtagagagt atagccaaaa cagtaataaa caatgacagt taaaatgtaa
     4381 agactatgaa aattacattg gaagggagct ttcaagatgg taggatattg actaactgag
     4441 ctccttcact taaaaatcag ctgaataaat tccttaagta tacttcaaga caagtgtaaa
     4501 ttgtttcaag ctgttaaagt taaacctaat aaccattatc aaggcaactc ctgggtaagt
     4561 aacacttaga accatcaaga gtggtttaag gggctggcta tacccattcc tctgtagagc
     4621 acagagaaat cctattaaaa aaaaaatact acatggtaat gaaatcttag cattgattga
     4681 gtgctttacg tgctgcctgc aagttttcag ttttgttgtt agagttttcg tctttatgaa
     4741 tgataaagtc ctatgttcat aaagtaacat ttaaattcct gaactaaaag gaaatgcctg
     4801 ttgtgcttaa aattttgggg agatacgaag tgaatgtgta aatctaactt tgaggttcag
     4861 taagttaagt agtatagacc tagagatcac tgctttacca ttttactttc aaatgaatga
     4921 gagcgactgc tagtggcaga acgcagaaaa ggggaaaaac aagccatgaa atgaagttgc
     4981 tttcctgacc tgggtttagg tcatcatttt aaaatcaaag atgtgtagga gatgaatcaa
     5041 cttatagacc catattttcc ttctaaaagt ttcccaaaga aatgttatag attttgaata
     5101 acatgccagt gacccagcag gtccaagatg gctaaccaag atggctaatt tagccacatt
     5161 gaataactta cttttaccct cacatggaaa gttggtatct attgctaaat gatttcaagg
     5221 tcactctgac agtctttgac actgtttttt ttagggtttt attgttttct ggtctcctgg
     5281 tgtctgtcct cagaattaaa gcatgtcctg catccctaag atgatagcca aggcctctct
     5341 tttattaaga gtcagcactg atgtagggta aaatcaacag tttttgcata tgattttaaa
     5401 atctttcttt gatttgaatt tgctttagtg tgtgcatgtt gagaggaagt aagtgtagaa
     5461 accaccctaa tttgtaagtt gggagttgga gatacttggt ggtttttcaa aaataaagac
     5521 tcaatctttt tttttttttt aaacactcac taccatctcc ttctaaaaca actctgtcag
     5581 tttaaatggc atataaaatg tgaacaaaca cctcattaca tgaagtcaaa atggtgaaga
     5641 gcttcagtga aacatttaaa gagtatattg tgtgttgttt tgctgttcat cattacttat
     5701 taaatgcagt aatgttgatt gcc
//
