LOCUS       NM_001064               2996 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens transketolase (TKT), transcript variant 1, mRNA.
ACCESSION   NM_001064
VERSION     NM_001064.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Geng,L., Zhu,M., Luo,D., Chen,H., Li,B., Lao,Y., An,H., Wu,Y.,
            Li,Y., Xia,A., Shi,Y., Tong,Z., Lu,S., Xu,D., Wang,X., Zhang,W.,
            Sun,B. and Xu,Z.
  TITLE     TKT-PARP1 axis induces radioresistance by promoting DNA
            double-strand break repair in hepatocellular carcinoma
  JOURNAL   Oncogene 43 (9), 682-692 (2024)
   PUBMED   38216672
  REMARK    GeneRIF: TKT-PARP1 axis induces radioresistance by promoting DNA
            double-strand break repair in hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Li,M., Zhao,X., Yong,H., Xu,J., Qu,P., Qiao,S., Hou,P., Li,Z.,
            Chu,S., Zheng,J. and Bai,J.
  TITLE     Transketolase promotes colorectal cancer metastasis through
            regulating AKT phosphorylation
  JOURNAL   Cell Death Dis 13 (2), 99 (2022)
   PUBMED   35110545
  REMARK    GeneRIF: Transketolase promotes colorectal cancer metastasis
            through regulating AKT phosphorylation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2996)
  AUTHORS   Nauton,L., Hecquet,L. and Thery,V.
  TITLE     QM/MM Study of Human Transketolase: Thiamine Diphosphate Activation
            Mechanism and Complete Catalytic Cycle
  JOURNAL   J Chem Inf Model 61 (7), 3502-3515 (2021)
   PUBMED   34161071
  REMARK    GeneRIF: QM/MM Study of Human Transketolase: Thiamine Diphosphate
            Activation Mechanism and Complete Catalytic Cycle.
REFERENCE   4  (bases 1 to 2996)
  AUTHORS   Mitschke,L., Parthier,C., Schroder-Tittmann,K., Coy,J., Ludtke,S.
            and Tittmann,K.
  TITLE     The crystal structure of human transketolase and new insights into
            its mode of action
  JOURNAL   J Biol Chem 285 (41), 31559-31570 (2010)
   PUBMED   20667822
  REMARK    GeneRIF: The crystal structure of human transketolase and new
            insights into its mode of action.
REFERENCE   5  (bases 1 to 2996)
  AUTHORS   Sax,C.M., Salamon,C., Kays,W.T., Guo,J., Yu,F.X., Cuthbertson,R.A.
            and Piatigorsky,J.
  TITLE     Transketolase is a major protein in the mouse cornea
  JOURNAL   J Biol Chem 271 (52), 33568-33574 (1996)
   PUBMED   8969223
REFERENCE   6  (bases 1 to 2996)
  AUTHORS   Usmanov,R.A., Sidorova,N.N. and Kochetov,G.A.
  TITLE     Interaction of dihydroxyethylthiamine pyrophosphate with
            transketolase
  JOURNAL   Biochem Mol Biol Int 38 (2), 307-314 (1996)
   PUBMED   8850526
REFERENCE   7  (bases 1 to 2996)
  AUTHORS   McCool,B.A., Plonk,S.G., Martin,P.R. and Singleton,C.K.
  TITLE     Cloning of human transketolase cDNAs and comparison of the
            nucleotide sequence of the coding region in Wernicke-Korsakoff and
            non-Wernicke-Korsakoff individuals
  JOURNAL   J Biol Chem 268 (2), 1397-1404 (1993)
   PUBMED   8419340
REFERENCE   8  (bases 1 to 2996)
  AUTHORS   Abedinia,M., Layfield,R., Jones,S.M., Nixon,P.F. and Mattick,J.S.
  TITLE     Nucleotide and predicted amino acid sequence of a cDNA clone
            encoding part of human transketolase
  JOURNAL   Biochem Biophys Res Commun 183 (3), 1159-1166 (1992)
   PUBMED   1567394
REFERENCE   9  (bases 1 to 2996)
  AUTHORS   Lapsys,N.M., Layfield,R., Baker,E., Callen,D.F., Sutherland,G.R.,
            Abedinia,M., Nixon,P.F. and Mattick,J.S.
  TITLE     Chromosomal location of the human transketolase gene
  JOURNAL   Cytogenet Cell Genet 61 (4), 274-275 (1992)
   PUBMED   1486804
REFERENCE   10 (bases 1 to 2996)
  AUTHORS   Mocali,A. and Paoletti,F.
  TITLE     Transketolase from human leukocytes. Isolation, properties and
            induction of polyclonal antibodies
  JOURNAL   Eur J Biochem 180 (1), 213-219 (1989)
   PUBMED   2495942
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB503248.1, BC009970.2,
            L12711.1, BC024026.2, CA421169.1 and AC097015.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001064.3.
            
            Summary: This gene encodes a thiamine-dependent enzyme which plays
            a role in the channeling of excess sugar phosphates to glycolysis
            in the pentose phosphate pathway. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Apr 2012].
            
            Transcript Variant: This variant (1) differs in the 3' UTR,
            compared to variant 2. Variants 1 and 2 encode the same isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223614.1, SRR3476690.321727.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154529 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000462138.6/ ENSP00000417773.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                DB503248.1         93-102
            11-544              BC009970.2         1-534
            545-1833            L12711.1           563-1851
            1834-1874           BC024026.2         1495-1535
            1875-2072           CA421169.1         16-213              c
            2073-2996           AC097015.2         83736-84659         c
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1"
     gene            1..2996
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /note="transketolase"
                     /db_xref="GeneID:7086"
                     /db_xref="HGNC:HGNC:11834"
                     /db_xref="MIM:606781"
     exon            1..187
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1952
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /EC_number="2.2.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     epididymis luminal protein 107; epididymis secretory
                     protein Li 48"
                     /codon_start=1
                     /product="transketolase isoform 1"
                     /protein_id="NP_001055.1"
                     /db_xref="CCDS:CCDS2871.1"
                     /db_xref="GeneID:7086"
                     /db_xref="HGNC:HGNC:11834"
                     /db_xref="MIM:606781"
                     /translation="MESYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSA
                     AEIMAVLFFHTMRYKSQDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLAEAELLNLRK
                     ISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCLLGDGELSE
                     GSVWEAMAFASIYKLDNLVAILDINRLGQSDPAPLQHQMDIYQKRCEAFGWHAIIVDG
                     HSVEELCKAFGQAKHQPTAIIAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYS
                     QIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDR
                     IIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAF
                     FTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSD
                     GVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGA
                     GVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEG
                     GIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA"
     exon            188..305
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..419
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..517
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..709
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            710..828
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            829..1022
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1187
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1344
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1475
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1559
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1653
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1776
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..2996
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2037..2042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /note="hexamer: AATATA"
     polyA_site      2060
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /note="major polyA site"
     regulatory      2977..2982
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
                     /note="hexamer: AATAAA"
     polyA_site      2996
                     /gene="TKT"
                     /gene_synonym="HEL-S-48; HEL107; SDDHD; TK; TKT1"
ORIGIN      
        1 ctcggcagcc gcctgtcgcc gcgggagcag ccgctatctc tgtgtgtccg cgtgtgcgcc
       61 cggtccccgc ctgccgcacc atggagagct accacaagcc tgaccagcag aagctgcagg
      121 ccttgaagga cacggccaac cgcctacgta tcagctccat ccaggccacc actgcggcgg
      181 gctctggcca ccccacgtca tgctgcagcg ccgcagagat catggctgtc ctctttttcc
      241 acaccatgcg ctacaagtcc caggaccccc ggaatccgca caatgaccgc tttgtgctct
      301 ccaagggcca tgcagctccc atcctctacg cggtctgggc tgaagctggt ttcctggccg
      361 aggcggagct gctgaacctg aggaagatca gctccgactt ggacgggcac ccggtcccga
      421 aacaagcttt caccgacgtg gccactggct ccctgggcca gggcctcggg gccgcttgtg
      481 ggatggccta caccggcaaa tacttcgaca aggccagcta ccgagtctat tgcttgctgg
      541 gagacgggga gctgtcagag ggctctgtat gggaggccat ggccttcgcc agcatctata
      601 agctggacaa ccttgtggcc attctagaca tcaatcgcct gggccagagt gacccggccc
      661 cactgcagca ccagatggac atctaccaga agcggtgcga ggccttcggt tggcatgcca
      721 tcatcgtgga tggacacagc gtggaggagc tgtgcaaggc ctttggccag gccaagcacc
      781 agccaacagc catcattgcc aagaccttca agggccgagg gatcacgggg gtagaagata
      841 aggagtcttg gcatgggaag cccctcccca aaaacatggc tgagcagatc atccaggaga
      901 tctacagcca gatccagagc aaaaagaaga tcctggcaac ccctccacag gaggacgcac
      961 cctcagtgga cattgccaac atccgcatgc ccagcctgcc cagctacaaa gttggggaca
     1021 agatagccac ccgcaaggcc tacgggcagg cactggccaa gctgggccat gccagtgacc
     1081 gcatcatcgc cctggatggg gacaccaaaa attccacctt ctcggagatc ttcaaaaagg
     1141 agcacccgga ccgcttcatc gagtgctaca ttgctgagca gaacatggtg agcatcgcgg
     1201 tgggctgtgc cacccgcaac aggacggtgc ccttctgcag cacttttgca gccttcttca
     1261 cgcgggcctt tgaccagatt cgcatggccg ccatctccga gagcaacatc aacctctgcg
     1321 gctcccactg cggcgtttcc atcggggaag acgggccctc ccagatggcc ctagaagatc
     1381 tggctatgtt tcggtcagtc cccacatcaa ctgtctttta cccaagtgat ggcgttgcta
     1441 cagagaaggc agtggaacta gccgccaata caaagggtat ctgcttcatc cggaccagcc
     1501 gcccagaaaa tgccatcatc tataacaaca atgaggactt ccaggtcgga caagccaagg
     1561 tggtcctgaa gagcaaggat gaccaggtga ccgttatcgg ggctggggtg accctgcacg
     1621 aggccttggc cgctgccgaa ctgctgaaga aagaaaagat caacatccgc gtgctggacc
     1681 ccttcaccat caagcccctg gacagaaaac tcattctcga cagcgctcgt gccaccaagg
     1741 gcaggatcct caccgtggag gaccattatt atgaaggtgg cattggtgag gctgtgtcca
     1801 gtgcagtagt gggcgagcct ggcatcactg tcacccacct ggcagttaac cgggtaccaa
     1861 gaagtgggaa gccggctgag ctgctgaaga tgtttggtat cgacagggat gccattgcac
     1921 aagctgtgag gggcctcatc accaaggcct agggcgggta tgaagtgtgg ggcgggggtc
     1981 tatacattcc tgagattctg ggaaaggtgc tcaaagatgt actgagagga ggggtaaata
     2041 tatgttttga gaaaaatgaa ttggccctga aatgttttct tttttgatgt gtttgcagcc
     2101 agtgcaggtt ctggggaaga agcgtggagg gtgcgctagg gaggctggtg tccttggtcc
     2161 tgggccagga cctgggtgtc ccaagtcccc ttggaatcac ctgcaactgc cctcaacctc
     2221 cagaactatc gctgcctccc attcatcacc aggtgacatt tgactgcaac ctgccttcta
     2281 gctgagaggc tgagacctac atccctcatt gtgacctcag tccacctggc cctgagcggg
     2341 ctggggaact gcctcagtct ggaagctgac caggcactct cagggccgcc ccacctcccc
     2401 caagtcccca cagccttgca gtcaggtctc ctgggatagg gaggttcact tgcttgttgt
     2461 ccctcgtcct tgtcatatcc ttttaactag gcatctcaga gaagcagaga cagggcagcc
     2521 ttcgtcctgg gggaaaaggg accctcagga tggcatgaga ggtcctcaat cccaagtgtg
     2581 gaactgtccc cctcaacttg ttaaaatgca gatttctggg tcttgccaat ggggcctggg
     2641 actccatgtg acaactggcc caggagcttc tgatgtcaca cagaattctg cagtcccaag
     2701 ctccagcccc gacctgctct gctgttccta ggtgactgcc ctcacactgc tgaccacagt
     2761 ggatttctcc ccctgctgct cgggctcagc tggggtcagc cctgcttata aggtcaactg
     2821 tgcaaaacct tatactggcc aagaacaaac tagtgctggg ggaggagggc tgggtgcccc
     2881 ggccactggt ggagtcccca ggaaatcctc agagctgttg cgaggatgag acacatttgt
     2941 ggacacgtcc acctgtcctc ctgaccgtct ggagagaata aacctgtcaa agccaa
//
