LOCUS       NM_003258               1431 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens thymidine kinase 1 (TK1), transcript variant 1, mRNA.
ACCESSION   NM_003258
VERSION     NM_003258.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Osredkar,J., Jagarlamudi,K.K., Cviic,D., Skof,E., Cvjeticanin,B.,
            Zore,A., Lukanovic,D., Eriksson,S. and Meglic,L.
  TITLE     Clinical Significance of the TK1-Specific Activity in the Early
            Detection of Ovarian Cancer
  JOURNAL   Oncology 102 (1), 17-29 (2024)
   PUBMED   37673047
  REMARK    GeneRIF: Clinical Significance of the TK1-Specific Activity in the
            Early Detection of Ovarian Cancer.
            Erratum:[Oncology. 2023;101(12):843. doi: 10.1159/000534436.. PMID:
            37903463]
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Bitter,E.E., Skidmore,J., Allen,C.I., Erickson,R.I., Morris,R.M.,
            Mortimer,T., Meade,A., Brog,R., Phares,T., Townsend,M.,
            Pickett,B.E. and O'Neill,K.L.
  TITLE     TK1 expression influences pathogenicity by cell cycle progression,
            cellular migration, and cellular survival in HCC 1806 breast cancer
            cells
  JOURNAL   PLoS One 18 (11), e0293128 (2023)
   PUBMED   38033034
  REMARK    GeneRIF: TK1 expression influences pathogenicity by cell cycle
            progression, cellular migration, and cellular survival in HCC 1806
            breast cancer cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1431)
  AUTHORS   Wu,M. and Ye,M.
  TITLE     Transcription factor Dp-1 knockdown downregulates thymidine kinase
            1 expression to protect against proliferation and
            epithelial-mesenchymal transition in cervical cancer
  JOURNAL   Funct Integr Genomics 23 (4), 301 (2023)
   PUBMED   37715794
  REMARK    GeneRIF: Transcription factor Dp-1 knockdown downregulates
            thymidine kinase 1 expression to protect against proliferation and
            epithelial-mesenchymal transition in cervical cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1431)
  AUTHORS   Tribukait,B., Lundgren,P.O., Kjellman,A., Norming,U., Nyman,C.R.,
            Jagarlmundi,K. and Gustafsson,O.
  TITLE     Prediction of Overall Survival by Thymidine Kinase 1 Combined with
            Prostate-Specific Antigen in Men with Prostate Cancer
  JOURNAL   Int J Mol Sci 24 (6), 5160 (2023)
   PUBMED   36982234
  REMARK    GeneRIF: Prediction of Overall Survival by Thymidine Kinase 1
            Combined with Prostate-Specific Antigen in Men with Prostate
            Cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1431)
  AUTHORS   Li,C.L., Lu,C.Y., Ke,P.Y. and Chang,Z.F.
  TITLE     Perturbation of ATP-induced tetramerization of human cytosolic
            thymidine kinase by substitution of serine-13 with aspartic acid at
            the mitotic phosphorylation site
  JOURNAL   Biochem Biophys Res Commun 313 (3), 587-593 (2004)
   PUBMED   14697231
  REMARK    GeneRIF: the enzymatic function at the G2/M phase of TK1 depends on
            its quaternary structure
REFERENCE   6  (bases 1 to 1431)
  AUTHORS   Chang,Z.F., Huang,D.Y. and Chi,L.M.
  TITLE     Serine 13 is the site of mitotic phosphorylation of human thymidine
            kinase
  JOURNAL   J Biol Chem 273 (20), 12095-12100 (1998)
   PUBMED   9575153
REFERENCE   7  (bases 1 to 1431)
  AUTHORS   Kim,Y.K. and Lee,A.S.
  TITLE     Identification of a protein-binding site in the promoter of the
            human thymidine kinase gene required for the G1-S-regulated
            transcription
  JOURNAL   J Biol Chem 267 (4), 2723-2727 (1992)
   PUBMED   1370831
REFERENCE   8  (bases 1 to 1431)
  AUTHORS   Munch-Petersen,B., Cloos,L., Tyrsted,G. and Eriksson,S.
  TITLE     Diverging substrate specificity of pure human thymidine kinases 1
            and 2 against antiviral dideoxynucleosides
  JOURNAL   J Biol Chem 266 (14), 9032-9038 (1991)
   PUBMED   2026611
REFERENCE   9  (bases 1 to 1431)
  AUTHORS   Barik,S. and Banerjee,A.K.
  TITLE     Cloning and expression of the vesicular stomatitis virus
            phosphoprotein gene in Escherichia coli: analysis of
            phosphorylation status versus transcriptional activity
  JOURNAL   J Virol 65 (4), 1719-1726 (1991)
   PUBMED   1848304
REFERENCE   10 (bases 1 to 1431)
  AUTHORS   Sadava,D. and Bernard,B.
  TITLE     Transition from cytosolic to mitochondrial thymidine kinase during
            development in human fetal tissues
  JOURNAL   Life Sci 47 (25), 2359-2364 (1990)
   PUBMED   2263164
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI754079.1 and BC006484.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003258.4.
            
            Summary: The protein encoded by this gene is a cytosolic enzyme
            that catalyzes the addition of a gamma-phosphate group to
            thymidine. This creates dTMP and is the first step in the
            biosynthesis of dTTP, which is one component required for DNA
            replication. The encoded protein, whose levels fluctuate depending
            on the cell cycle stage, can act as a low activity dimer or a high
            activity tetramer. High levels of this protein have been used as a
            biomarker for diagnosing and categorizing many types of cancers.
            [provided by RefSeq, Oct 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006484.1, K02581.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:9575153)
            MANE Ensembl match               :: ENST00000301634.12/
                                                ENSP00000301634.6
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BI754079.1         183-200
            19-1431             BC006484.1         1-1413
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..1431
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /note="thymidine kinase 1"
                     /db_xref="GeneID:7083"
                     /db_xref="HGNC:HGNC:11830"
                     /db_xref="MIM:188300"
     exon            1..124
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..763
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /EC_number="2.7.1.21"
                     /note="isoform 1 is encoded by transcript variant 1;
                     thymidine kinase, cytosolic; epididymis secretory sperm
                     binding protein; cytosolic thymidine kinase"
                     /codon_start=1
                     /product="thymidine kinase, cytosolic isoform 1"
                     /protein_id="NP_003249.3"
                     /db_xref="CCDS:CCDS11754.1"
                     /db_xref="GeneID:7083"
                     /db_xref="HGNC:HGNC:11830"
                     /db_xref="MIM:188300"
                     /translation="MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQ
                     IAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFF
                     PDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREA
                     AYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAA
                     RKLFAPQQILQCSPAN"
     exon            125..156
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     exon            157..267
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     exon            268..361
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     exon            362..451
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     exon            452..571
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     exon            572..1431
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1431
                     /gene="TK1"
                     /gene_synonym="TK2"
                     /note="major polyA site"
ORIGIN      
        1 ggcttactgc gggacggcct tggagagtac tcgggttcgt gaacttcccg gaggcgcaat
       61 gagctgcatt aacctgccca ctgtgctgcc tggctccccc agcaagaccc gggggcagat
      121 ccaggtgatt ctcgggccga tgttctcagg aaaaagcaca gagttgatga gacgcgtccg
      181 tcgcttccag attgctcagt acaagtgcct ggtgatcaag tatgccaaag acactcgcta
      241 cagcagcagc ttctgcacac atgaccggaa caccatggag gcactgcccg cctgcctgct
      301 ccgagacgtg gcccaggagg ccctgggcgt ggctgtcata ggcatcgacg aggggcagtt
      361 tttccctgac atcgtggagt tctgcgaggc catggccaac gccgggaaga ccgtaattgt
      421 ggctgcactg gatgggacct tccagaggaa gccatttggg gccatcctga acctggtgcc
      481 gctggccgag agcgtggtga agctgacggc ggtgtgcatg gagtgcttcc gggaagccgc
      541 ctataccaag aggctcggca cagagaagga ggtcgaggtg attgggggag cagacaagta
      601 ccactccgtg tgtcggctct gctacttcaa gaaggcctca ggccagcctg ccgggccgga
      661 caacaaagag aactgcccag tgccaggaaa gccaggggaa gccgtggctg ccaggaagct
      721 ctttgcccca cagcagattc tgcaatgcag ccctgccaac tgagggacct gcgagggccg
      781 cccgctccct tcctgccact gccgcctact ggacgctgcc ctgcatgctg cccagccact
      841 ccaggaggaa gtcgggaggc gtggagggtg accacacctt ggccttctgg gaactctcct
      901 ttgtgtggct gccccacctg ccgcatgctc cctcctctcc tacccactgg tctgcttaaa
      961 gcttccctct cagctgctgg gacgatcgcc caggctggag ctggccccgc ttggtggcct
     1021 gggatctggc acactccctc tccttggggt gagggacaga gccccacgct gttgacatca
     1081 gcctgcttct tcccctctgc ggctttcact gctgagtttc tgttctccct gggaagcctg
     1141 tgccagcacc tttgagcctt ggcccacact gaggcttagg cctctctgcc tgggatgggc
     1201 tcccaccctc ccctgaggat ggcctggatt cacgccctct tgtttccttt tgggctcaaa
     1261 gcccttccta cctctggtga tggtttccac aggaacaaca gcatctttca ccaagatggg
     1321 tggcaccaac cttgctggga cttggatccc aggggcttat ctcttcaagt gtggagaggg
     1381 cagggtccac gcctctgctg tagcttatga aattaactaa ttgaaaattc a
//
