LOCUS       NM_004817               4503 bp    mRNA    linear   PRI 16-FEB-2025
DEFINITION  Homo sapiens tight junction protein 2 (TJP2), transcript variant 1,
            mRNA.
ACCESSION   NM_004817
VERSION     NM_004817.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ouyang,H., Wu,S., Li,W., Grey,M.J., Wu,W. and Hansen,S.H.
  TITLE     p120 RasGAP and ZO-2 are essential for Hippo signaling and
            tumor-suppressor function mediated by p190A RhoGAP
  JOURNAL   Cell Rep 42 (12), 113486 (2023)
   PUBMED   37995182
  REMARK    GeneRIF: p120 RasGAP and ZO-2 are essential for Hippo signaling and
            tumor-suppressor function mediated by p190A RhoGAP.
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Gallego-Gutierrez,H., Gonzalez-Gonzalez,L., Ramirez-Martinez,L.,
            Lopez-Bayghen,E. and Gonzalez-Mariscal,L.
  TITLE     Tight junction protein ZO-2 modulates the nuclear accumulation of
            transcription factor TEAD
  JOURNAL   Mol Biol Cell 32 (15), 1347-1358 (2021)
   PUBMED   34010016
  REMARK    GeneRIF: Tight junction protein ZO-2 modulates the nuclear
            accumulation of transcription factor TEAD.
REFERENCE   3  (bases 1 to 4503)
  AUTHORS   Wei,C.S., Becher,N., Friis,J.B., Ott,P., Vogel,I. and Gronbaek,H.
  TITLE     New tight junction protein 2 variant causing progressive familial
            intrahepatic cholestasis type 4 in adults: A case report
  JOURNAL   World J Gastroenterol 26 (5), 550-561 (2020)
   PUBMED   32089630
  REMARK    GeneRIF: New tight junction protein 2 variant causing progressive
            familial intrahepatic cholestasis type 4 in adults: A case report.
REFERENCE   4  (bases 1 to 4503)
  AUTHORS   Walsh,T., Pierce,S.B., Lenz,D.R., Brownstein,Z.,
            Dagan-Rosenfeld,O., Shahin,H., Roeb,W., McCarthy,S., Nord,A.S.,
            Gordon,C.R., Ben-Neriah,Z., Sebat,J., Kanaan,M., Lee,M.K.,
            Frydman,M., King,M.C. and Avraham,K.B.
  TITLE     Genomic duplication and overexpression of TJP2/ZO-2 leads to
            altered expression of apoptosis genes in progressive nonsyndromic
            hearing loss DFNA51
  JOURNAL   Am J Hum Genet 87 (1), 101-109 (2010)
   PUBMED   20602916
  REMARK    GeneRIF: TJP2- and GSK-3beta-mediated increased susceptibility to
            apoptosis of cells of the inner ear is the mechanism for
            adult-onset hearing loss in this kindred and may serve as one model
            for age-related hearing loss in the general population.
REFERENCE   5  (bases 1 to 4503)
  AUTHORS   Itoh,M., Morita,K. and Tsukita,S.
  TITLE     Characterization of ZO-2 as a MAGUK family member associated with
            tight as well as adherens junctions with a binding affinity to
            occludin and alpha catenin
  JOURNAL   J Biol Chem 274 (9), 5981-5986 (1999)
   PUBMED   10026224
REFERENCE   6  (bases 1 to 4503)
  AUTHORS   Denker,B.M. and Nigam,S.K.
  TITLE     Molecular structure and assembly of the tight junction
  JOURNAL   Am J Physiol 274 (1), F1-F9 (1998)
   PUBMED   9458817
  REMARK    Review article
REFERENCE   7  (bases 1 to 4503)
  AUTHORS   Beatch,M., Jesaitis,L.A., Gallin,W.J., Goodenough,D.A. and
            Stevenson,B.R.
  TITLE     The tight junction protein ZO-2 contains three PDZ
            (PSD-95/Discs-Large/ZO-1) domains and an alternatively spliced
            region
  JOURNAL   J Biol Chem 271 (42), 25723-25726 (1996)
   PUBMED   8824195
REFERENCE   8  (bases 1 to 4503)
  AUTHORS   Duclos,F., Rodius,F., Wrogemann,K., Mandel,J.L. and Koenig,M.
  TITLE     The Friedreich ataxia region: characterization of two novel genes
            and reduction of the critical region to 300 kb
  JOURNAL   Hum Mol Genet 3 (6), 909-914 (1994)
   PUBMED   7951235
REFERENCE   9  (bases 1 to 4503)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
REFERENCE   10 (bases 1 to 4503)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB090695.1, L27476.1,
            BC027592.1 and AL358113.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 11, 2018 this sequence version replaced NM_004817.3.
            
            Summary: This gene encodes a zonula occluden that is a member of
            the membrane-associated guanylate kinase homolog family. The
            encoded protein functions as a component of the tight junction
            barrier in epithelial and endothelial cells and is necessary for
            proper assembly of tight junctions. Mutations in this gene have
            been identified in patients with hypercholanemia, and genomic
            duplication of a 270 kb region including this gene causes autosomal
            dominant deafness-51. Alternatively spliced transcripts encoding
            multiple isoforms have been observed for this gene. [provided by
            RefSeq, Nov 2011].
            
            Transcript Variant: This variant (1) encodes isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027592.1, L27476.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377245.9/ ENSP00000366453.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DB090695.1         224-240
            18-1327             L27476.1           1-1310
            1328-1871           BC027592.1         1441-1984
            1872-1872           AL358113.21        70261-70261
            1873-2447           BC027592.1         1986-2560
            2448-2448           AL358113.21        74504-74504
            2449-4069           BC027592.1         2562-4182
            4070-4503           AL358113.21        90493-90926
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.11"
     gene            1..4503
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /note="tight junction protein 2"
                     /db_xref="GeneID:9414"
                     /db_xref="HGNC:HGNC:11828"
                     /db_xref="MIM:607709"
     exon            1..156
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..3669
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Friedreich ataxia region gene X104 (tight junction protein
                     ZO-2); zona occludens 2; zonula occludens protein 2"
                     /codon_start=1
                     /product="tight junction protein 2 isoform 1"
                     /protein_id="NP_004808.2"
                     /db_xref="CCDS:CCDS6627.1"
                     /db_xref="GeneID:9414"
                     /db_xref="HGNC:HGNC:11828"
                     /db_xref="MIM:607709"
                     /translation="MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGF
                     GIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAV
                     QQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSR
                     LNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRG
                     RSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHD
                     ARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTR
                     TGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINI
                     PSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLS
                     RMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGP
                     NTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGL
                     VREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLA
                     FTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDR
                     ADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIA
                     DIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAV
                     DLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTA
                     TINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSC
                     DSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQ
                     MRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGA
                     STKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKM
                     DHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAP
                     SRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL"
     exon            157..210
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            211..335
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..438
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            439..1048
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1152
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1306
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1415
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1549
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1616
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1767
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1876
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2087
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2275
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2371
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2451
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2662
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2763
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2764..2976
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3087
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3417
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3503
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..4503
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4471..4476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /note="hexamer: AATAAA"
     regulatory      4481..4486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /note="hexamer: AATACA"
     polyA_site      4492
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
     polyA_site      4499
                     /gene="TJP2"
                     /gene_synonym="C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;
                     PFIC4; X104; ZO2"
                     /note="major polyA site"
ORIGIN      
        1 agagcactgt ccggtggtgc ccaggaggag taggagcagg agcagaagca gaagcggggt
       61 ccggagctgc gcgcctacgc gggacctgtg tccgaaatgc cggtgcgagg agaccgcggg
      121 tttccacccc ggcgggagct gtcaggttgg ctccgcgccc caggcatgga agagctgata
      181 tgggaacagt acactgtgac cctacaaaag gattccaaaa gaggatttgg aattgcagtg
      241 tccggaggca gagacaaccc ccactttgaa aatggagaaa cgtcaattgt catttctgat
      301 gtgctcccgg gtgggcctgc tgatgggctg ctccaagaaa atgacagagt ggtcatggtc
      361 aatggcaccc ccatggagga tgtgcttcat tcgtttgcag ttcagcagct cagaaaaagt
      421 gggaaggtcg ctgctattgt ggtcaagagg ccccggaagg tccaggtggc cgcacttcag
      481 gccagccctc ccctggatca ggatgaccgg gcttttgagg tgatggacga gtttgatggc
      541 agaagtttcc ggagtggcta cagcgagagg agccggctga acagccatgg ggggcgcagc
      601 cgcagctggg aggacagccc ggaaaggggg cgtccccatg agcgggcccg gagccgggag
      661 cgggacctca gccgggaccg gagccgtggc cggagcctgg agcggggcct ggaccaagac
      721 catgcgcgca cccgagaccg cagccgtggc cggagcctgg agcggggcct ggaccacgac
      781 tttgggccat cccgggaccg ggaccgtgac cgcagccgcg gccggagcat tgaccaggac
      841 tacgagcgag cctatcaccg ggcctacgac ccagactacg agcgggccta cagcccggag
      901 tacaggcgcg gggcccgcca cgatgcccgc tctcggggac cccgaagccg cagccgcgag
      961 cacccgcact cacggagccc cagccccgag cctagggggc ggccggggcc catcggggtc
     1021 ctcctgatga aaagcagagc gaacgaagag tatggtctcc ggcttgggag tcagatcttc
     1081 gtaaaggaaa tgacccgaac gggtctggca actaaagatg gcaaccttca cgaaggagac
     1141 ataattctca agatcaatgg gactgtaact gagaacatgt ctttaacgga tgctcgaaaa
     1201 ttgatagaaa agtcaagagg aaaactacag ctagtggtgt tgagagacag ccagcagacc
     1261 ctcatcaaca tcccgtcatt aaatgacagt gactcagaaa tagaagatat ttcagaaata
     1321 gagtcaaacc gatcattttc tccagaggag agacgtcatc agtattctga ttatgattat
     1381 cattcctcaa gtgagaagct gaaggaaagg ccaagttcca gagaggacac gccgagcaga
     1441 ttgtccagga tgggtgcgac acccactccc tttaagtcca caggggatat tgcaggcaca
     1501 gttgtcccag agaccaacaa ggaacccaga taccaagagg accccccagc tcctcaacca
     1561 aaagcagccc cgagaacttt tcttcgtcct agtcctgaag atgaagcaat atatggccct
     1621 aataccaaaa tggtaaggtt caagaaggga gacagcgtgg gcctccggtt ggctggtggc
     1681 aatgatgtcg ggatatttgt tgctggcatt caagaaggga cctcggcgga gcaggagggc
     1741 cttcaagaag gagaccagat tctgaaggtg aacacacagg atttcagagg attagtgcgg
     1801 gaggatgccg ttctctacct gttagaaatc cctaaaggtg aaatggtgac cattttagct
     1861 cagagccgag ccgatgtgta tagagacatc ctggcttgtg gcagagggga ttcgtttttt
     1921 ataagaagcc actttgaatg tgagaaggaa actccacaga gcctggcctt caccagaggg
     1981 gaggtcttcc gagtggtaga cacactgtat gacggcaagc tgggcaactg gctggctgtg
     2041 aggattggga acgagttgga gaaaggctta atccccaaca agagcagagc tgaacaaatg
     2101 gccagtgttc aaaatgccca gagagacaac gctggggacc gggcagattt ctggagaatg
     2161 cgtggccaga ggtctggggt gaagaagaac ctgaggaaaa gtcgggaaga cctcacagct
     2221 gttgtgtctg tcagcaccaa gttcccagct tatgagaggg ttttgctgcg agaagctggt
     2281 ttcaagagac ctgtggtctt attcggcccc atagctgata tagcaatgga aaaattggct
     2341 aatgagttac ctgactggtt tcaaactgct aaaacggaac caaaagatgc aggatctgag
     2401 aaatccactg gagtggtccg gttaaatacc gtgaggcaaa ttattgaaca ggataagcat
     2461 gcactactgg atgtgactcc gaaagctgtg gacctgttga attacaccca gtggttccca
     2521 attgtgattt ttttcaaccc agactccaga caaggtgtca aaaccatgag acaaaggtta
     2581 aatccaacgt ccaacaaaag ttctcgaaag ttatttgatc aagccaacaa gcttaaaaaa
     2641 acgtgtgcac acctttttac agctacaatc aacctaaatt cagccaatga tagctggttt
     2701 ggcagcttaa aggacactat tcagcatcag caaggagaag cggtttgggt ctctgaagga
     2761 aagatggaag ggatggatga tgaccccgaa gaccgcatgt cctacttaac cgccatgggc
     2821 gcggactatc tgagttgcga cagccgcctc atcagtgact ttgaagacac ggacggtgaa
     2881 ggaggcgcct acactgacaa tgagctggat gagccagccg aggagccgct ggtgtcgtcc
     2941 atcacccgct cctcggagcc ggtgcagcac gaggagagca taaggaaacc cagcccagag
     3001 ccacgagctc agatgaggag ggctgctagc agcgatcaac ttagggacaa tagcccgccc
     3061 ccagcattca agccagagcc gcccaaggcc aaaacccaga acaaagaaga atcctatgac
     3121 ttctccaaat cctatgaata taagtcaaac ccctctgccg ttgctggtaa tgaaactcct
     3181 ggggcatcta ccaaaggtta tcctcctcct gttgcagcaa aacctacctt tgggcggtct
     3241 atactgaagc cctccactcc catccctcct caagagggtg aggaggtggg agagagcagt
     3301 gaggagcaag ataatgctcc caaatcagtc ctgggcaaag tcaaaatatt tgagaagatg
     3361 gatcacaagg ccaggttaca gagaatgcag gagctccagg aagcacagaa tgcaaggatc
     3421 gaaattgccc agaagcatcc tgatatctat gcagttccaa tcaaaacgca caagccagac
     3481 cctggcacgc cccagcacac gagttccaga ccccctgagc cacagaaagc tccttccaga
     3541 ccttatcagg ataccagagg aagttatggc agtgatgccg aggaggagga gtaccgccag
     3601 cagctgtcag aacactccaa gcgcggttac tatggccagt ctgcccgata ccgggacaca
     3661 gaattataga tgtctgagca cggactctcc caggcctgcc tgcatggcat cagactagcc
     3721 actcctgcca ggccgccggg atggttcttc tccagttaga atgcaccatg gagacgtggt
     3781 gggactccag ctcgtgtgtc ctcatggaga acccagggga cagctggtgc aaattcagaa
     3841 ctgagggctc tgtttgtggg actgggttag aggagtctgt ggctttttgt tcagaattaa
     3901 gcagaacact gcagtcagat cctgttactt gcttcagtgg accgaaatct gtattctgtt
     3961 tgcgtacttg taatatgtat attaagaagc aataactatt tttcctcatt aatagctgcc
     4021 ttcaaggact gtttcagtgt gagtcagaat gtgaaaaagg aataaaaaat actgttgggc
     4081 tcaaactaaa ttcaaagaag tactttattg caactctttt aagtgccttg gatgagaagt
     4141 gtcttaaatt ttcttccttt gaagctttag gcagagccat aatggactaa aacattttga
     4201 ctaagttttt ataccagctt aatagctgta gttttccctg cactgtgtca tcttttcaag
     4261 gcatttgtct ttgtaatatt ttccataaat ttggactgtc tatatcataa ctatacttga
     4321 tagtttggct ataagtgctc aatagcttga agcccaagaa gttggtatcg aaatttgttg
     4381 tttgtttaaa cccaagtgct gcacaaaagc agatacttga ggaaaacact atttccaaaa
     4441 gcacatgtat tgacaacagt tttataattt aataaaaagg aatacattgc aatccgtaat
     4501 ttt
//
