LOCUS       NM_001330239            7190 bp    mRNA    linear   PRI 16-FEB-2025
DEFINITION  Homo sapiens tight junction protein 1 (TJP1), transcript variant 5,
            mRNA.
ACCESSION   NM_001330239 XM_005254618
VERSION     NM_001330239.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Si,W., Liu,J., Wang,Y., Mao,Y., Zhang,Y., Xu,S., Guo,K., Zhang,Y.,
            Hu,Y. and Zhang,F.
  TITLE     IL-8 promotes lens capsular residual cells migration by
            down-regulates expression of E-cadherin and ZO-1 via the
            CXCR1/2-NF-kappaB-RhoA signal pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113074 (2024)
   PUBMED   39244903
  REMARK    GeneRIF: IL-8 promotes lens capsular residual cells migration by
            down-regulates expression of E-cadherin and ZO-1 via the
            CXCR1/2-NF-kappaB-RhoA signal pathway.
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Miki,R., Matsuo,S., Ushida,T., Tano,S., Imai,K., Nawa,A.,
            Kajiyama,H. and Kotani,T.
  TITLE     TJP1 suppresses trophoblast cell invasion by expressing E2F8 in the
            human placenta
  JOURNAL   Mol Cell Endocrinol 591, 112277 (2024)
   PUBMED   38795825
  REMARK    GeneRIF: TJP1 suppresses trophoblast cell invasion by expressing
            E2F8 in the human placenta.
REFERENCE   3  (bases 1 to 7190)
  AUTHORS   Liu,Y., Qin,Y. and Zhang,Y.
  TITLE     circRNA-PTPN4 mediated regulation of FOXO3 and ZO-1 expression:
            implications for blood-brain barrier integrity and cognitive
            function in uremic encephalopathy
  JOURNAL   Cell Biol Toxicol 40 (1), 22 (2024)
   PUBMED   38630149
  REMARK    GeneRIF: circRNA-PTPN4 mediated regulation of FOXO3 and ZO-1
            expression: implications for blood-brain barrier integrity and
            cognitive function in uremic encephalopathy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7190)
  AUTHORS   Tornavaca,O., Chia,M., Dufton,N., Almagro,L.O., Conway,D.E.,
            Randi,A.M., Schwartz,M.A., Matter,K. and Balda,M.S.
  TITLE     ZO-1 controls endothelial adherens junctions, cell-cell tension,
            angiogenesis, and barrier formation
  JOURNAL   J Cell Biol 208 (6), 821-838 (2015)
   PUBMED   25753039
  REMARK    GeneRIF: ZO-1 is a central regulator of VE-cadherin-dependent
            endothelial junctions that orchestrates the spatial actomyosin
            organization.
REFERENCE   5  (bases 1 to 7190)
  AUTHORS   Runkle,E.A. and Mu,D.
  TITLE     Tight junction proteins: from barrier to tumorigenesis
  JOURNAL   Cancer Lett 337 (1), 41-48 (2013)
   PUBMED   23743355
  REMARK    Review article
REFERENCE   6  (bases 1 to 7190)
  AUTHORS   Nomme,J., Fanning,A.S., Caffrey,M., Lye,M.F., Anderson,J.M. and
            Lavie,A.
  TITLE     The Src homology 3 domain is required for junctional adhesion
            molecule binding to the third PDZ domain of the scaffolding protein
            ZO-1
  JOURNAL   J Biol Chem 286 (50), 43352-43360 (2011)
   PUBMED   22030391
  REMARK    GeneRIF: The Src homology 3 domain is required for junctional
            adhesion molecule binding to the third PDZ domain of the
            scaffolding protein ZO-1.
REFERENCE   7  (bases 1 to 7190)
  AUTHORS   Van Itallie,C.M., Balda,M.S. and Anderson,J.M.
  TITLE     Epidermal growth factor induces tyrosine phosphorylation and
            reorganization of the tight junction protein ZO-1 in A431 cells
  JOURNAL   J Cell Sci 108 (Pt 4), 1735-1742 (1995)
   PUBMED   7542259
REFERENCE   8  (bases 1 to 7190)
  AUTHORS   Furuse,M., Itoh,M., Hirase,T., Nagafuchi,A., Yonemura,S.,
            Tsukita,S. and Tsukita,S.
  TITLE     Direct association of occludin with ZO-1 and its possible
            involvement in the localization of occludin at tight junctions
  JOURNAL   J Cell Biol 127 (6 Pt 1), 1617-1626 (1994)
   PUBMED   7798316
REFERENCE   9  (bases 1 to 7190)
  AUTHORS   Balda,M.S. and Anderson,J.M.
  TITLE     Two classes of tight junctions are revealed by ZO-1 isoforms
  JOURNAL   Am J Physiol 264 (4 Pt 1), C918-C924 (1993)
   PUBMED   7682777
REFERENCE   10 (bases 1 to 7190)
  AUTHORS   Willott,E., Balda,M.S., Heintzelman,M., Jameson,B. and
            Anderson,J.M.
  TITLE     Localization and differential expression of two isoforms of the
            tight junction protein ZO-1
  JOURNAL   Am J Physiol 262 (5 Pt 1), C1119-C1124 (1992)
   PUBMED   1590354
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022613.13.
            
            On Jun 1, 2020 this sequence version replaced NM_001330239.3.
            
            Summary: This gene encodes a member of the membrane-associated
            guanylate kinase (MAGUK) family of proteins, and acts as a tight
            junction adaptor protein that also regulates adherens junctions.
            Tight junctions regulate the movement of ions and macromolecules
            between endothelial and epithelial cells. The multidomain structure
            of this scaffold protein, including a postsynaptic density
            95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3)
            domain, a guanylate kinase (GuK) domain and unique (U) motifs all
            help to co-ordinate binding of transmembrane proteins, cytosolic
            proteins, and F-actin, which are required for tight junction
            function. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Aug
            2017].
            
            Transcript Variant: This variant (5) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. The
            encoded isoform (e) is longer than isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.1073263.1,
                                           SRR14038197.278075.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000614355.5/ ENSP00000483470.2
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-467               AC022613.13        177507-177973       c
            468-524             AC022613.13        156151-156207       c
            525-649             AC022613.13        128738-128862       c
            650-752             AC022613.13        127569-127671       c
            753-1029            AC022613.13        121771-122047       c
            1030-1133           AC022613.13        117840-117943       c
            1134-1302           AC022613.13        117106-117274       c
            1303-1450           AC022613.13        116644-116791       c
            1451-1590           AC022613.13        98148-98287         c
            1591-1696           AC022613.13        96837-96942         c
            1697-1847           AC022613.13        92770-92920         c
            1848-1956           AC022613.13        89780-89888         c
            1957-2176           AC022613.13        88600-88819         c
            2177-2361           AC022613.13        88137-88321         c
            2362-2457           AC022613.13        87939-88034         c
            2458-2540           AC022613.13        83443-83525         c
            2541-2751           AC022613.13        82287-82497         c
            2752-2852           AC022613.13        81885-81985         c
            2853-3203           AC022613.13        75864-76214         c
            3204-3443           AC022613.13        75283-75522         c
            3444-4316           AC022613.13        73772-74644         c
            4317-4414           AC022613.13        73527-73624         c
            4415-4642           AC022613.13        72117-72344         c
            4643-4812           AC022613.13        66337-66506         c
            4813-5290           AC022613.13        64065-64542         c
            5291-5508           AC022613.13        61034-61251         c
            5509-5652           AC022613.13        59668-59811         c
            5653-7190           AC022613.13        55658-57195         c
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q13.1"
     gene            1..7190
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /note="tight junction protein 1"
                     /db_xref="GeneID:7082"
                     /db_xref="HGNC:HGNC:11827"
                     /db_xref="MIM:601009"
     exon            1..467
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             441..5747
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /note="isoform e is encoded by transcript variant 5; zona
                     occludens 1; zonula occludens 1 protein; tight junction
                     protein ZO-1"
                     /codon_start=1
                     /product="tight junction protein 1 isoform e"
                     /protein_id="NP_001317168.1"
                     /db_xref="CCDS:CCDS81858.1"
                     /db_xref="GeneID:7082"
                     /db_xref="HGNC:HGNC:11827"
                     /db_xref="MIM:601009"
                     /translation="MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDN
                     PHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNA
                     KITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRS
                     ASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAA
                     RDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSD
                     SIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPS
                     NGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQV
                     GQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGV
                     LEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYR
                     RIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNH
                     KEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQ
                     PVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTD
                     QRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMR
                     MRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLV
                     WVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQ
                     ELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPG
                     FKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQAD
                     SLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHR
                     PDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYE
                     EQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRY
                     EQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPP
                     QGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQ
                     SVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHD
                     KTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAAS
                     HLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYA
                     QPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTA
                     QAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNL
                     LPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTV
                     PKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVE
                     QEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGW
                     SFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF"
     exon            468..524
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            525..649
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            650..752
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            753..1029
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1133
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1302
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1450
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1590
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1696
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1847
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..1956
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2176
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2361
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..2457
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..2540
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2751
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2752..2852
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..3203
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3443
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3444..4316
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4317..4414
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4415..4642
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4812
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4813..5290
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5291..5508
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5509..5652
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     exon            5653..7190
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7170..7175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /note="hexamer: AATAAA"
     polyA_site      7190
                     /gene="TJP1"
                     /gene_synonym="ZO-1"
                     /note="major polyA site"
ORIGIN      
        1 gtggccgctg agttgccggc gccggctgag ccagcggacg ccgcgttcct tggcggccgc
       61 cggttcccgg gaagttacgt ggcgaagccg gcttccgagg agacgccggg aggccacggg
      121 tgctgctgac gggcgggcga ccgggcgagg ccgacgtggc cgggctgcga aagctgcggg
      181 aggccgagtg ggtggccgcg ctcggaggga ggtgccggtc gggcgcgccc cgtggagaag
      241 acccgggcgg ggcgggcgct tcccggactt ttgtccgagt tgaattccct ccccctgggc
      301 cgggcccttc cggccgcccc cgcccgtgcc ccgctcgctc tcgggagatg tttatttggg
      361 ctgtggcgtg aggagcgggc gggccagcgc cgcggagttt cgggtccgag gagcctcgcg
      421 cggcgctgga gagagacaag atgtccgcca gagctgcggc cgccaagagc acagcaatgg
      481 aggaaacagc tatatgggaa caacatacag tgacgcttca cagggctcct ggatttggat
      541 ttggaattgc aatatctggt ggacgagata atcctcattt tcagagtggg gaaacgtcaa
      601 tagtgatttc agatgtgctg aaaggaggac cagctgaagg acagctacag gaaaatgacc
      661 gagttgcaat ggttaacgga gtttcaatgg ataatgttga acatgctttt gctgttcagc
      721 aactaaggaa aagtgggaaa aatgcaaaaa ttacaattag aaggaagaag aaagttcaaa
      781 taccagtaag tcgtcctgat cctgaaccag tatctgataa tgaagaagat agttatgatg
      841 aggaaataca tgatccaaga agtggccgga gtggtgtggt taacagaagg agtgagaaga
      901 tttggccgag ggatagaagt gcaagtagag agaggagctt gtccccgcgg tcagacaggc
      961 ggtcagtggc ttccagccag cctgctaaac ctactaaagt cacactggtg aaatcccgga
     1021 aaaatgaaga atatggtctt cgattggcaa gccatatatt tgttaaggaa atttcacaag
     1081 atagtttggc agcaagagat ggcaatattc aagaaggtga tgttgtattg aagataaatg
     1141 gtactgtgac agaaaatatg tcattgacag atgcaaagac attgatagaa aggtctaaag
     1201 gcaaattaaa aatggtagtt caaagagatg aacgggctac gctattgaat gtccctgatc
     1261 tttctgacag catccactct gctaatgcct ctgagagaga cgacatttca gaaattcagt
     1321 cactggcatc agatcattct ggtcgatcac acgataggcc tccccgccgc agccggtcac
     1381 gatctcctga ccagcggtca gagccttctg atcattccag gcactcgccg cagcagccaa
     1441 gcaatggcag tctccggagt agagatgaag agagaatttc taaacctggg gctgtctcaa
     1501 ctcctgtaaa gcatgctgat gatcacacac ctaaaacagt ggaagaagtt acagttgaaa
     1561 gaaatgagaa acaaacacct tctcttccag aaccaaagcc tgtgtatgcc caagttgggc
     1621 aaccagatgt ggatttacct gtcagtccat ctgatggtgt cctacctaat tcaactcatg
     1681 aagatgggat tcttcggccc agcatgaaat tggtaaaatt cagaaaagga gatagtgtgg
     1741 gtttgcggct ggctggtgga aatgatgttg gaatatttgt agctggcgtt ctagaagata
     1801 gccctgcagc caaggaaggc ttagaggaag gtgatcaaat tctcagggta aacaacgtag
     1861 attttacaaa tatcataaga gaagaagccg tccttttcct gcttgacctc cctaaaggag
     1921 aagaagtgac catattggct cagaagaaga aggatgttta tcgtcgcatt gtagaatcag
     1981 atgtaggaga ttctttctat attagaaccc attttgaata tgaaaaggaa tctccctatg
     2041 gacttagttt taacaaagga gaggtgttcc gtgttgtgga taccttgtac aatggaaaac
     2101 tgggctcttg gcttgctatt cgaattggta aaaatcataa ggaggtagaa cgaggcatca
     2161 tccctaataa gaacagagct gagcagctag ccagtgtaca gtatacactt ccaaaaacag
     2221 caggcggaga ccgtgctgac ttctggagat tcagaggtct tcgcagctcc aagagaaatc
     2281 ttcgaaaaag cagagaggat ttgtccgctc agcctgttca aacaaagttt ccagcttatg
     2341 aaagagtggt tcttcgagaa gctggatttc tgaggcctgt aaccattttt ggaccaatag
     2401 ctgatgttgc cagagaaaag ctggcaagag aagaaccaga tatttatcaa attgcaaaga
     2461 gtgaaccacg agacgctgga actgaccaac gtagctctgg cattattcgc ctgcatacaa
     2521 taaagcaaat catagatcaa gacaaacatg ctttattaga tgtaacacca aatgcagttg
     2581 atcgtcttaa ctatgcccag tggtatccaa ttgttgtatt tcttaaccct gattctaagc
     2641 aaggagtaaa aacaatgaga atgaggttat gtccagaatc tcggaaaagt gccaggaagt
     2701 tatacgagcg atctcataaa cttcgtaaaa ataatcacca tctttttaca actacaatta
     2761 acttaaattc aatgaatgat ggttggtatg gtgcgctgaa agaagcaatt caacaacagc
     2821 aaaaccagct ggtatgggtt tccgagggaa aggcggatgg tgctacaagt gatgaccttg
     2881 atttgcatga tgatcgtctg tcctacctgt cagctccagg tagtgaatac tcaatgtata
     2941 gcacggacag tagacacact tctgactatg aagacacaga cacagaaggc ggggcctaca
     3001 ctgatcaaga actagatgaa actcttaatg atgaggttgg gactccaccg gagtctgcca
     3061 ttacacggtc ctctgagcct gtaagagagg actcctctgg aatgcatcat gaaaaccaaa
     3121 catatcctcc ttactcacca caagcgcagc cacaaccaat tcatagaata gactcccctg
     3181 gatttaagcc agcctctcaa cagaaagcag aagcttcatc tccagtccct tacctttcgc
     3241 ctgaaacaaa cccagcatca tcaacctctg ctgttaatca taatgtaaat ttaactaatg
     3301 tcagactgga ggagcccacc ccagctcctt ccacctctta ctcaccacaa gctgattctt
     3361 taagaacacc aagtactgag gcagctcaca taatgctaag agatcaagaa ccatcattgt
     3421 cgtcgcatgt agatccaaca aaggtgtata gaaaggatcc atatcccgag gaaatgatga
     3481 ggcagaacca tgttttgaaa cagccagccg ttagtcaccc agggcacagg ccagacaaag
     3541 agcctaatct gacctatgaa ccccaactcc catacgtaga gaaacaagcc agcagagacc
     3601 tcgagcagcc cacatacaga tacgagtcct caagctatac ggaccagttt tctcgaaact
     3661 atgaacatcg tctgcgatac gaagatcgcg tccccatgta tgaagaacag tggtcatatt
     3721 atgatgacaa acagccctac ccatctcggc caccttttga taatcagcac tctcaagacc
     3781 ttgactccag acagcatccc gaagagtcct cagaacgagg gtactttcca cgttttgaag
     3841 agccagcccc tctgtcttac gacagcagac cacgttacga acaggcacct agagcatccg
     3901 ccctgcggca cgaagagcag ccagctcctg ggtatgacac acatggtaga ctcagaccgg
     3961 aagcccagcc ccacccttca gcagggccca agcctgcaga gtccaagcag tattttgagc
     4021 aatattcacg cagttacgag caagtaccac cccaaggatt tacctctaga gcaggtcatt
     4081 ttgagcctct ccatggtgct gcagctgtcc ctccgctgat accttcatct cagcataagc
     4141 cagaagctct gccttcaaac accaaaccac tgcctccacc cccaactcaa accgaagaag
     4201 aggaagatcc agcaatgaag ccacagtctg tactcaccag agttaagatg tttgaaaaca
     4261 aaagatctgc atccttagag accaagaagg atgtaaatga cactggcagt tttaagcctc
     4321 cagaagtagc atctaaacct tcaggtgctc ccatcattgg tcccaaaccc acttctcaga
     4381 atcaattcag tgaacatgac aaaactctgt acaggatccc agaacctcaa aaacctcaac
     4441 tgaagccacc tgaagatatt gttcggtcca atcattatga ccctgaagaa gatgaagaat
     4501 attatcgaaa acagctgtca tactttgacc gaagaagttt tgagaataag cctcctgcac
     4561 acattgccgc cagccatctc tccgagcctg caaagccagc gcattctcag aatcaatcaa
     4621 atttttctag ttattcttca aagggaaagc ctcctgaagc tgatggtgtg gatagatcat
     4681 ttggcgagaa acgctatgaa cccatccagg ccactccccc tcctcctcca ttgccctcgc
     4741 agtatgccca gccatctcag cctgtcacca gcgcgtctct ccacatacat tctaagggag
     4801 cacatggtga aggtaattca gtgtcattgg attttcagaa ttccttagtg tccaaaccag
     4861 acccacctcc atctcagaat aagccagcaa ctttcagacc accaaaccga gaagatactg
     4921 ctcaggcagc tttctatccc cagaaaagtt ttccagataa agccccagtt aatggaactg
     4981 aacagactca gaaaacagtc actccagcat acaatcgatt cacaccaaaa ccatatacaa
     5041 gttctgcccg accatttgaa cgcaagtttg aaagtcctaa attcaatcac aatcttctgc
     5101 caagtgaaac tgcacataaa cctgacttgt cttcaaaaac tcccacttct ccaaaaactc
     5161 ttgtgaaatc gcacagtttg gcacagcctc ctgagtttga cagtggagtt gaaactttct
     5221 ctatccatgc agagaagcct aaatatcaaa taaataatat cagcacagtg cctaaagcta
     5281 ttcctgtgag tccttcagct gtggaagagg atgaagatga agatggtcat actgtggtgg
     5341 ccacagcccg aggcatattt aacagcaatg ggggcgtgct gagttccata gaaactggtg
     5401 ttagtataat tatccctcaa ggagccattc ccgaaggagt tgagcaggaa atctatttca
     5461 aggtctgccg ggacaacagc atccttccac ctttagataa agagaaaggt gaaacactgc
     5521 tgagtccttt ggtgatgtgt ggtccccatg gcctcaagtt cctgaagcct gtggagctgc
     5581 gcttaccaca ctgtgcgtcc atgactcctg acggttggtc ttttgctcta aaatcatccg
     5641 actcctcgtc gggtgatcct aaaacctggc aaaacaagtg tcttcccgga gatccaaatt
     5701 atctcgttgg agcaaactgt gtttctgtcc ttattgacca cttttaactc ttgaaatata
     5761 ggaacttaaa taatgtgaaa ctggattaaa cttaatctaa atggaaccac tctatcaagt
     5821 attatacctt ttttagagtt gatactacag tttgttagta tgaggcattt gtttgaactg
     5881 ataaagatga gtgagcatgc ccctgaacca tggtcggaaa acatgctaca cactgcatgt
     5941 ttgtgattga cgggactgtt ggtattggct agaggttcaa agatattttg ctttgtgatt
     6001 tttgtaattt ttttatcgtc actgcttaac ttcacatatt gatttccgtt aaaataccag
     6061 ccagtaaatg ggggtgcatt tgaggtctgt tctttccaaa gtacactgtt tcaaacttta
     6121 ctatggccct ggcctagcat acgtacacat tttattttat tatgcatgaa gtaatatgca
     6181 cacatttttt aaatgcacct ggaatatata accagtgttg tggatttaac agaaatgtac
     6241 agcaaggaga tttacaactg ggggagggtg aagtgaagac aatgacttac tgtacatgaa
     6301 aacacatttt tcttagggaa ggatacaaaa gcatgtgaga ctggttccat ggcctcttca
     6361 gatctctaac ttcaccatat taccacagac atactaacca gcagaaatgc cttaccctca
     6421 tgttcttaat tcttagctca ttctccttgt gttactaagt ttttatggct tttgtgcatt
     6481 atctagatac tgtatcatga caaagactga gtacgttgtg catttggtgg tttcagaaat
     6541 gtgttatcac ccagaagaaa atagtggtgt gatttgggga tatttttttc ttttcttttc
     6601 ttttcttttt tttttttttt gacaaggggc agtggtggtt ttctgttctt tctggctatg
     6661 catttgaaaa ttttgatgtt ttaaggatgc ttgtacataa tgcgtgcata ccacttttgt
     6721 tcttggtttg taaattaact tttataaact ttaccttttt tatacataaa caagaccacg
     6781 tttctaaagg ctacctttgt attctctcct gtacctcttg agccttgaac tttgacctct
     6841 gcagcaataa agcagcgttt ctatgacaca tgcaaggtca ttttttttaa gaaaaaggat
     6901 gcacagagtt gttacatttt taagtgctgc atttaaaaga tacagttact cagaattctc
     6961 tagtttgatt aaattcttgc aaagtatccc tactgtaatt tgtgatacaa tgctgtgccc
     7021 taaagtgtat ttttttacta atagacaatt tattatggca catcagcacg atttctgttt
     7081 agataataca ccactacatt ctgttaatca ttaggtgtga ctgaatttct tttgccgtta
     7141 ttaaaaatct caaatttcta aatctccaaa ataaaacttt ttaaaataaa
//
