LOCUS       NM_012460               1252 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens translocase of inner mitochondrial membrane 9 (TIMM9),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_012460
VERSION     NM_012460.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1252)
  AUTHORS   Qi,L., Wang,Q., Guan,Z., Wu,Y., Shen,C., Hong,S., Cao,J., Zhang,X.,
            Yan,C. and Yin,P.
  TITLE     Cryo-EM structure of the human mitochondrial translocase TIM22
            complex
  JOURNAL   Cell Res 31 (3), 369-372 (2021)
   PUBMED   32901109
REFERENCE   4  (bases 1 to 1252)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   5  (bases 1 to 1252)
  AUTHORS   Lin,C.C., Fang,C.L., Sun,D.P., Hseu,Y.C., Uen,Y.H., Lin,K.Y. and
            Lin,Y.C.
  TITLE     High expression of mitochondrial intermembrane chaperone TIMM9
            represents a negative prognostic marker in gastric cancer
  JOURNAL   J Formos Med Assoc 116 (6), 476-483 (2017)
   PUBMED   27720672
  REMARK    GeneRIF: in gastric cancer patients, a borderline association was
            found between overexpression of TIMM9 and vascular invasion;
            patients with high expression levels of TIMM9 achieved a
            significantly lower disease-free survival rate compared with those
            with low expression levels
REFERENCE   6  (bases 1 to 1252)
  AUTHORS   Muhlenbein,N., Hofmann,S., Rothbauer,U. and Bauer,M.F.
  TITLE     Organization and function of the small Tim complexes acting along
            the import pathway of metabolite carriers into mammalian
            mitochondria
  JOURNAL   J Biol Chem 279 (14), 13540-13546 (2004)
   PUBMED   14726512
  REMARK    GeneRIF: in contrast to yeast, only a small fraction of
            Tim9-Tim10a-Tim10b complex is in a stable association with Tim22
REFERENCE   7  (bases 1 to 1252)
  AUTHORS   Vial,S., Lu,H., Allen,S., Savory,P., Thornton,D., Sheehan,J. and
            Tokatlidis,K.
  TITLE     Assembly of Tim9 and Tim10 into a functional chaperone
  JOURNAL   J Biol Chem 277 (39), 36100-36108 (2002)
   PUBMED   12138093
REFERENCE   8  (bases 1 to 1252)
  AUTHORS   Rothbauer,U., Hofmann,S., Muhlenbein,N., Paschen,S.A.,
            Gerbitz,K.D., Neupert,W., Brunner,M. and Bauer,M.F.
  TITLE     Role of the deafness dystonia peptide 1 (DDP1) in import of human
            Tim23 into the inner membrane of mitochondria
  JOURNAL   J Biol Chem 276 (40), 37327-37334 (2001)
   PUBMED   11489896
REFERENCE   9  (bases 1 to 1252)
  AUTHORS   Bauer,M.F., Rothbauer,U., Muhlenbein,N., Smith,R.J., Gerbitz,K.,
            Neupert,W., Brunner,M. and Hofmann,S.
  TITLE     The mitochondrial TIM22 preprotein translocase is highly conserved
            throughout the eukaryotic kingdom
  JOURNAL   FEBS Lett 464 (1-2), 41-47 (1999)
   PUBMED   10611480
REFERENCE   10 (bases 1 to 1252)
  AUTHORS   Jin,H., Kendall,E., Freeman,T.C., Roberts,R.G. and Vetrie,D.L.
  TITLE     The human family of Deafness/Dystonia peptide (DDP) related
            mitochondrial import proteins
  JOURNAL   Genomics 61 (3), 259-267 (1999)
   PUBMED   10552927
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA530575.1, AL139021.6 and BC020213.1.
            
            On Nov 23, 2018 this sequence version replaced NM_012460.3.
            
            Summary: TIMM9 belongs to a family of evolutionarily conserved
            proteins that are organized in heterooligomeric complexes in the
            mitochondrial intermembrane space. These proteins mediate the
            import and insertion of hydrophobic membrane proteins into the
            mitochondrial inner membrane.[supplied by OMIM, Apr 2004].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1 through 4 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.114649.1,
                                           SRR1163655.479490.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149004, SAMEA2155371
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000395159.7/
                                                  ENSP00000378588.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA530575.1         84-92
            10-17               AL139021.6         91161-91168         c
            18-287              BC020213.1         1-270
            288-310             AL139021.6         90719-90741         c
            311-1095            BC020213.1         294-1078
            1096-1252           AL139021.6         72141-72297         c
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.1"
     gene            1..1252
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="translocase of inner mitochondrial membrane 9"
                     /db_xref="GeneID:26520"
                     /db_xref="HGNC:HGNC:11819"
                     /db_xref="MIM:607384"
     exon            1..140
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     exon            141..329
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     exon            330..417
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     exon            418..482
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     CDS             444..713
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="isoform a is encoded by transcript variant 1;
                     mitochondrial import inner membrane translocase subunit
                     Tim9; translocase of inner mitochondrial membrane 9
                     homolog"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim9 isoform a"
                     /protein_id="NP_036592.1"
                     /db_xref="CCDS:CCDS9735.1"
                     /db_xref="GeneID:26520"
                     /db_xref="HGNC:HGNC:11819"
                     /db_xref="MIM:607384"
                     /translation="MAAQIPESDQIKQFKEFLGTYNKLTETCFLDCVKDFTTREVKPE
                     ETTCSEHCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR"
     exon            483..578
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     exon            579..1252
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      982..987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="hexamer: AATAAA"
     polyA_site      1002
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="major polyA site"
     regulatory      1079..1084
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="hexamer: AATAAA"
     polyA_site      1099
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="major polyA site"
     regulatory      1229..1234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
                     /note="hexamer: CATAAA"
     polyA_site      1252
                     /gene="TIMM9"
                     /gene_synonym="TIM9; TIM9A"
ORIGIN      
        1 aaggtagcac attgccgcac aagaccgctc tacagtctca tccaagactt gaagagctcc
       61 cgacttgtaa tactacttgt gtttaccagg gactgttgac actgcttcgc ttccgaagac
      121 cgttagggac gtgggattag atttcctcgc gttataacga atcggcgtct aagggtttaa
      181 gtgtcacttg acattactgc tcccacgtgt tctttcccat ctggcggccg cggctcctgt
      241 ccagaccctg accctccctc ccaaggctca accgtccccc aacaaccccc agccttgtac
      301 tgatgtcgga tgcgagagcc tgtgcttaag taagaatcag gccttattgg agacattcaa
      361 gcaaaggttg gacaactact tttccagaac agaaaggaaa ctcatgcatc agaaaaggtg
      421 actaataaag gtaccagaag aatatggctg cacaaatacc agaatctgat cagataaaac
      481 agtttaagga atttctgggg acctacaata aacttacaga gacctgcttt ttggactgtg
      541 ttaaagactt cacaacaaga gaagtaaaac ctgaagagac cacctgttca gaacattgct
      601 tacagaaata tttaaaaatg acacaaagaa tatccatgag atttcaggaa tatcatattc
      661 agcagaatga agccctggca gccaaagcag gactccttgg ccaaccacga tagagaagtc
      721 ctgatggatg aacttttgat gaaagattgc caacagctgc tttattggaa atgaggactc
      781 atctgataga atcccctgaa agcagtagcc accatgttca accatctgtc atgactgttt
      841 ggcaaatgga aaccgctgga gaaacaaaat tgctatttac caggaataat cacaatagaa
      901 ggtcttattg ttcagtgaaa taataagatg caacatttgt tgaggcctta tgattcagca
      961 gcttggtcac ttgattagaa aaataaacca ttgtttcttc aattgtgact gttaatttta
     1021 aagcaactta tgtgttcgat catgtatgag atagaaaaat ttttattact caaagtaaaa
     1081 taaatggaaa tatcactgag cagttgttta tactgtatgg taccatagta ttctcactgg
     1141 actgtggata cgttgaaatg tttttcaatc aggatcatcc tccaagcagt cctgttcatt
     1201 catgtctgcc tcaaattgct ggtgaataca taaaatagtt acttgctaat ca
//
