LOCUS       NM_006327               1190 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens translocase of inner mitochondrial membrane 23
            (TIMM23), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_006327
VERSION     NM_006327.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Akabane,S., Watanabe,K., Kosako,H., Yamashita,S.I., Nishino,K.,
            Kato,M., Sekine,S., Kanki,T., Matsuda,N., Endo,T. and Oka,T.
  TITLE     TIM23 facilitates PINK1 activation by safeguarding against
            OMA1-mediated degradation in damaged mitochondria
  JOURNAL   Cell Rep 42 (5), 112454 (2023)
   PUBMED   37160114
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Pandey,J., Larson-Casey,J.L., Patil,M.H., Joshi,R., Jiang,C.S.,
            Zhou,Y., He,C. and Carter,A.B.
  TITLE     NOX4-TIM23 interaction regulates NOX4 mitochondrial import and
            metabolic reprogramming
  JOURNAL   J Biol Chem 299 (5), 104695 (2023)
   PUBMED   37044213
  REMARK    GeneRIF: NOX4-TIM23 interaction regulates NOX4 mitochondrial import
            and metabolic reprogramming.
REFERENCE   3  (bases 1 to 1190)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 1190)
  AUTHORS   Barchiesi,A., Bazzani,V., Tolotto,V., Elancheliyan,P.,
            Wasilewski,M., Chacinska,A. and Vascotto,C.
  TITLE     Mitochondrial Oxidative Stress Induces Rapid Intermembrane
            Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1
            Protein through TIM23 Complex
  JOURNAL   J Mol Biol 432 (24), 166713 (2020)
   PUBMED   33197464
  REMARK    GeneRIF: Mitochondrial Oxidative Stress Induces Rapid Intermembrane
            Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1
            Protein through TIM23 Complex.
REFERENCE   5  (bases 1 to 1190)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 1190)
  AUTHORS   Muhlenbein,N., Hofmann,S., Rothbauer,U. and Bauer,M.F.
  TITLE     Organization and function of the small Tim complexes acting along
            the import pathway of metabolite carriers into mammalian
            mitochondria
  JOURNAL   J Biol Chem 279 (14), 13540-13546 (2004)
   PUBMED   14726512
REFERENCE   7  (bases 1 to 1190)
  AUTHORS   Yamamoto,H., Esaki,M., Kanamori,T., Tamura,Y., Nishikawa,Si. and
            Endo,T.
  TITLE     Tim50 is a subunit of the TIM23 complex that links protein
            translocation across the outer and inner mitochondrial membranes
  JOURNAL   Cell 111 (4), 519-528 (2002)
   PUBMED   12437925
REFERENCE   8  (bases 1 to 1190)
  AUTHORS   Moro,F., Sirrenberg,C., Schneider,H.C., Neupert,W. and Brunner,M.
  TITLE     The TIM17.23 preprotein translocase of mitochondria: composition
            and function in protein transport into the matrix
  JOURNAL   EMBO J 18 (13), 3667-3675 (1999)
   PUBMED   10393182
REFERENCE   9  (bases 1 to 1190)
  AUTHORS   Bauer,M.F., Gempel,K., Reichert,A.S., Rappold,G.A., Lichtner,P.,
            Gerbitz,K.D., Neupert,W., Brunner,M. and Hofmann,S.
  TITLE     Genetic and structural characterization of the human mitochondrial
            inner membrane translocase
  JOURNAL   J Mol Biol 289 (1), 69-82 (1999)
   PUBMED   10339406
REFERENCE   10 (bases 1 to 1190)
  AUTHORS   Prashar,Y. and Weissman,S.M.
  TITLE     Analysis of differential gene expression by display of 3' end
            restriction fragments of cDNAs
  JOURNAL   Proc Natl Acad Sci U S A 93 (2), 659-663 (1996)
   PUBMED   8570611
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC062707.1, BC066951.1 and
            BU619060.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006327.3.
            
            Summary: The protein encoded by this gene is part of a complex
            located in the inner mitochondrial membrane that mediates the
            transport of transit peptide-containing proteins across the
            membrane. Multiple transcript variants, one protein-coding and
            others not protein-coding, have been found for this gene. [provided
            by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) is the protein-coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.189847.1,
                                           SRR5189658.75577.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000580018.4/
                                                  ENSP00000464522.3
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BC062707.1         41-54
            15-1016             BC066951.1         1-1002
            1017-1190           BU619060.1         14-187              c
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.22"
     gene            1..1190
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /note="translocase of inner mitochondrial membrane 23"
                     /db_xref="GeneID:100287932"
                     /db_xref="HGNC:HGNC:17312"
                     /db_xref="MIM:605034"
     exon            1..242
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..766
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /note="translocase of the inner mitochondrial membrane;
                     translocase of inner mitochondrial membrane 23 homolog"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim23"
                     /protein_id="NP_006318.1"
                     /db_xref="CCDS:CCDS73091.1"
                     /db_xref="GeneID:100287932"
                     /db_xref="HGNC:HGNC:17312"
                     /db_xref="MIM:605034"
                     /translation="MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSP
                     YLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLKE
                     TQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTVAA
                     GTMTGMLYKCTGGLRGIARGGLTGLTLTSLYALYNNWEHMKGSLLQQSL"
     exon            243..301
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     exon            302..395
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     exon            396..480
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     exon            481..539
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     exon            540..650
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     exon            651..1190
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1145..1150
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /note="hexamer: AATAAA"
     polyA_site      1190
                     /gene="TIMM23"
                     /gene_synonym="TIM23"
                     /note="major polyA site"
ORIGIN      
        1 aaccggcgcc cggaaggtca gcgtgtgaag taggcgctgg caacgcgggg ttacccgctg
       61 ttattgagga gtaacggccc agcggaccac ccaggcttga ggcagcggcg ggaaccactc
      121 ggtttgctgc gataccatgg aaggaggcgg gggaagcggc aacaaaacca cagggggatt
      181 ggccggcttt ttcggagccg gcggagcagg ttactcgcac gcggatttgg ctggcgtccc
      241 gctaactggt atgaaccctc tgtctcctta tttaaatgtg gatccacgat acctcgtgca
      301 ggatacagat gagtttattt tacctaccgg agctaataaa acccggggca gatttgagct
      361 ggccttcttt acgattggag gatgttgcat gacaggggct gcgtttggtg caatgaatgg
      421 tcttcggcta ggattgaagg aaacccagaa catggcctgg tccaaaccaa gaaatgtaca
      481 gattttgaat atggtgacta ggcaaggggc actttgggct aatactctag gttctctggc
      541 tttgctctat agtgcatttg gtgtcatcat tgagaaaaca cgaggtgcag aagatgacct
      601 taacacagta gcagctggaa ccatgacagg catgttgtat aaatgtacag gtggtcttcg
      661 agggatagca cgaggtggtc tgacaggact aacacttacc agcctctatg cactatataa
      721 taactgggag cacatgaaag gctccttgct ccaacagtca ctctgaagat tttgccaact
      781 catgaatgga ggacacttca gtagtcatct agatcctttt ataagacagt ttggagttat
      841 tctctctctt ctacctacaa ttagtttgaa aaattggaga ttttgatttg ctgtgatgaa
      901 aatcctggat ggctgaccaa gactggcact tgttccagcc attagtgagt tgaagccaaa
      961 gccctttggt gactcactga gtaccatggt tctgttctcc tctggagatc ttgcacgtat
     1021 ctgttttcct cccccatgaa ctagaaaacc acttactccc agaattcagg tcgtgcttgt
     1081 tagtactata tcaccaagtc cattcattta atgatccaaa actgtaatgt tgcactgtat
     1141 tccaaataaa gggtaaaaac agaaccaaag ttataactcc aacacacaaa
//
