LOCUS       NM_013337               3182 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens translocase of inner mitochondrial membrane 22
            (TIMM22), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_013337
VERSION     NM_013337.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Guo,L., Liu,J.J., Long,S.Y., Wang,P.Y., Li,S., Wang,J.L., Wei,X.F.,
            Li,J., Lei,L., Huang,A.L. and Hu,J.L.
  TITLE     TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1
            expression
  JOURNAL   J Med Virol 96 (2), e29439 (2024)
   PUBMED   38294104
  REMARK    GeneRIF: TIM22 and TIM29 inhibit HBV replication by up-regulating
            SRSF1 expression.
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 3182)
  AUTHORS   Jackson,T.D., Hock,D.H., Fujihara,K.M., Palmer,C.S., Frazier,A.E.,
            Low,Y.C., Kang,Y., Ang,C.S., Clemons,N.J., Thorburn,D.R.,
            Stroud,D.A. and Stojanovski,D.
  TITLE     The TIM22 complex mediates the import of sideroflexins and is
            required for efficient mitochondrial one-carbon metabolism
  JOURNAL   Mol Biol Cell 32 (6), 475-491 (2021)
   PUBMED   33476211
  REMARK    GeneRIF: The TIM22 complex mediates the import of sideroflexins and
            is required for efficient mitochondrial one-carbon metabolism.
REFERENCE   4  (bases 1 to 3182)
  AUTHORS   Qi,L., Wang,Q., Guan,Z., Wu,Y., Shen,C., Hong,S., Cao,J., Zhang,X.,
            Yan,C. and Yin,P.
  TITLE     Cryo-EM structure of the human mitochondrial translocase TIM22
            complex
  JOURNAL   Cell Res 31 (3), 369-372 (2021)
   PUBMED   32901109
  REMARK    GeneRIF: Cryo-EM structure of the human mitochondrial translocase
            TIM22 complex.
REFERENCE   5  (bases 1 to 3182)
  AUTHORS   Valpadashi,A., Callegari,S., Linden,A., Neumann,P., Ficner,R.,
            Urlaub,H., Deckers,M. and Rehling,P.
  TITLE     Defining the architecture of the human TIM22 complex by chemical
            crosslinking
  JOURNAL   FEBS Lett 595 (2), 157-168 (2021)
   PUBMED   33125709
  REMARK    GeneRIF: Defining the architecture of the human TIM22 complex by
            chemical crosslinking.
REFERENCE   6  (bases 1 to 3182)
  AUTHORS   Callegari,S., Richter,F., Chojnacka,K., Jans,D.C., Lorenzi,I.,
            Pacheu-Grau,D., Jakobs,S., Lenz,C., Urlaub,H., Dudek,J.,
            Chacinska,A. and Rehling,P.
  TITLE     TIM29 is a subunit of the human carrier translocase required for
            protein transport
  JOURNAL   FEBS Lett 590 (23), 4147-4158 (2016)
   PUBMED   27718247
REFERENCE   7  (bases 1 to 3182)
  AUTHORS   Kang,Y., Baker,M.J., Liem,M., Louber,J., McKenzie,M., Atukorala,I.,
            Ang,C.S., Keerthikumar,S., Mathivanan,S. and Stojanovski,D.
  TITLE     Tim29 is a novel subunit of the human TIM22 translocase and is
            involved in complex assembly and stability
  JOURNAL   Elife 5, e17463 (2016)
   PUBMED   27554484
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3182)
  AUTHORS   Muhlenbein,N., Hofmann,S., Rothbauer,U. and Bauer,M.F.
  TITLE     Organization and function of the small Tim complexes acting along
            the import pathway of metabolite carriers into mammalian
            mitochondria
  JOURNAL   J Biol Chem 279 (14), 13540-13546 (2004)
   PUBMED   14726512
  REMARK    GeneRIF: in contrast to yeast, only a small fraction of
            Tim9-Tim10a-Tim10b complex is in a stable association with Tim22.
REFERENCE   9  (bases 1 to 3182)
  AUTHORS   Kovermann,P., Truscott,K.N., Guiard,B., Rehling,P., Sepuri,N.B.,
            Muller,H., Jensen,R.E., Wagner,R. and Pfanner,N.
  TITLE     Tim22, the essential core of the mitochondrial protein insertion
            complex, forms a voltage-activated and signal-gated channel
  JOURNAL   Mol Cell 9 (2), 363-373 (2002)
   PUBMED   11864609
REFERENCE   10 (bases 1 to 3182)
  AUTHORS   Bauer,M.F., Rothbauer,U., Muhlenbein,N., Smith,R.J., Gerbitz,K.,
            Neupert,W., Brunner,M. and Hofmann,S.
  TITLE     The mitochondrial TIM22 preprotein translocase is highly conserved
            throughout the eukaryotic kingdom
  JOURNAL   FEBS Lett 464 (1-2), 41-47 (1999)
   PUBMED   10611480
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC002324.2, AI522232.1 and
            AC015884.15.
            
            On Nov 23, 2018 this sequence version replaced NM_013337.3.
            
            Summary: Multipass transmembrane proteins are brought into
            mitochondria and inserted into the mitochondrial inner membrane by
            way of the TIM22 complex. This complex has six subunits and is a
            twin-pore translocase. The protein encoded by this gene is a
            subunit of TIM22 and represents the voltage-activated and
            signal-gated channel. [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.162958.1,
                                           SRR1660807.72389.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000327158.5/
                                                  ENSP00000320236.2
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1678              BC002324.2         13-1690
            1679-1694           AI522232.1         8-23                c
            1695-3182           AC015884.15        51680-53167
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..3182
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="translocase of inner mitochondrial membrane 22"
                     /db_xref="GeneID:29928"
                     /db_xref="HGNC:HGNC:17317"
                     /db_xref="MIM:607251"
     exon            1..252
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..599
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="testis-expressed sequence 4; translocase of inner
                     mitochondrial membrane 22 homolog; testis-expressed
                     protein 4"
                     /codon_start=1
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim22"
                     /protein_id="NP_037469.2"
                     /db_xref="CCDS:CCDS32521.1"
                     /db_xref="GeneID:29928"
                     /db_xref="HGNC:HGNC:17317"
                     /db_xref="MIM:607251"
                     /translation="MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEP
                     GSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDP
                     KDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGC
                     ITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR"
     exon            253..449
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /inference="alignment:Splign:2.1.0"
     exon            450..522
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /inference="alignment:Splign:2.1.0"
     exon            523..3182
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1104..1109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="hexamer: AATACA"
     polyA_site      1147
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="major polyA site"
     polyA_site      1616
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
     regulatory      1657..1662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="hexamer: ATTAAA"
     polyA_site      1699
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
     regulatory      2515..2520
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="hexamer: AATAAA"
     polyA_site      2534
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
     polyA_site      3060
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
     regulatory      3161..3166
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
                     /note="hexamer: ATTAAA"
     polyA_site      3182
                     /gene="TIMM22"
                     /gene_synonym="COXPD43; TEX4; TIM22"
ORIGIN      
        1 gggcagcgac tgtcatggcg gcggccgccc ccaatgccgg aggctcggcc cctgagacag
       61 cgggttccgc cgaagctccg ctgcagtaca gcctgctcct gcagtacctg gtgggtgaca
      121 agcgtcagcc ccggctcctg gagcctggga gcctgggcgg gatcccaagt ccagccaaga
      181 gtgaggagca gaagatgatc gagaaggcga tggaaagctg cgctttcaag gctgcgctgg
      241 cctgcgtggg aggatttgtc ttaggaggtg catttggggt gtttaccgct ggcatcgata
      301 ccaacgtggg ctttgaccct aaggatcctt accgtacacc gactgcaaaa gaagtgctga
      361 aagacatggg gcagagagga atgtcctatg ccaaaaattt cgccattgtg ggagccatgt
      421 tttcttgtac tgagtgtttg atagaatctt accggggaac atcagactgg aagaacagtg
      481 tcatcagtgg ctgcatcacg ggaggagcta ttggtttcag agctggctta aaggctgggg
      541 ccattggttg tggaggtttt gctgctttct ctgctgcgat tgattattac ctccggtgag
      601 agtaattgcc tgcagggaag gatgatgcca gccccggatc cgggctgctc tctggaggac
      661 agtttctgta ccacaccagg gccttgcttc agggcctgaa gacattcatt ttccctcatg
      721 tcgttggtat tctgagggag ctgcctggct tctctgcctc cagcctttgg ggtagccaca
      781 ctttgctgct cctggactcc agccagcctt cacagaggac gtcccgtgcc agattctctc
      841 acagcagatc gggagacagg atgttgacat ataggaattc agctccacaa agcttcaggc
      901 ctgaccacag ctggccctct aggttgtttg gtgttgtggg cacagaggtg acagtgtctc
      961 tgcaggcact caggaagctg ttctaccttg gaactccatg caactatccg tctcgaatac
     1021 cagggcaggg caaggcggac tgatgccgtc caaccaatta tctcacttgt tttgtgttct
     1081 gtcatccttg tttttttttt aaaaatacac tcccctcccc caccgccaca caccttacag
     1141 ttcaaaaggt tggtttattt gtattttatt ttcaaattcc ctattccaaa aatagtgccg
     1201 ggtggcccaa agataacatc tgaagggaaa gaatgagaaa gctcccaggg aagcggggga
     1261 tggggcgctg aggcagggtt gttagaggac tgtgtcatcc tcaccaagct cattacatct
     1321 gccagggcct gcctcaagaa gcccagccca gccttttcct gggatgcagt gaaatcccat
     1381 ccatgaactc gatgggcccc tcctgtgccg gccgagaggc acacactgcc ttcacgacgt
     1441 gactgctgga cctggccgag cttgaggcca catgtgaagg gtcctgctgt ggtcatcttg
     1501 gtgactcggt cacagcagct actggccaag atcagacgtc gctgaggggc tgttcaccac
     1561 catcctcgtt ctccagggtc aaggaagtgt tttaacatgt cgtgttttcg acttgacctt
     1621 gtggtatttt tcttggccta gttgtgtgcc atggatatta aaaaaaaaaa aaaaaaaatc
     1681 cgtctacagt tgggcttcag cactggtgct gctttaccct ggtgctctgt ggggggatga
     1741 cactgctgga gtcaggcctt tgctcttggg tggggcacag gcaggtcgct tggtgggggc
     1801 cacacagtgc tgggcacctt cagccagtgg tgatgagtct cctttttcct ataatacggt
     1861 aattcctcta tcctttcctt ttttgccttc ttcctcatct gccctgtctt ctggcccaca
     1921 cactcttaac cagcgttcac actcagtgta catggcctgg aggcccgagt gtttgtacat
     1981 gagtgatgat gtcaaaccca gctggtaaca ccttccttgg gtcatgtttg ccattttctt
     2041 ggaatgaatg tgagttcctg ctcagggctc atgtcctttt acagtgaatt ctatataacg
     2101 cccctcccag tctcacagct aggaggcttc atcactgcta ggccagttgg agcgttccct
     2161 agagctcaga acaaattgtt tcctctgctg tccctaaata taggacacct acaagcactc
     2221 tgaagcaagg gcagacattc ccacctggta cctgtcaaag tcctaggatg cctgggatct
     2281 tccatctttc agtctagcac gtgggaccaa atacaagaga tgctgccctc acaacagcct
     2341 tggaaaagat gagcgccagg gctgtcagta cccatcggtt cagtaagcga ggcattgtcc
     2401 acgctgccta ttcactcgag agatgaatag tttcctgttt tcgatggctg gggagccagt
     2461 atgagctcat aaaccaaaca gcaattttca gagacatctg ttcctgatct tcagaataaa
     2521 ctcagtgtcc agttgcttcg gctggtggga gccaatattc acgccactga ctctctcaaa
     2581 gggagggtgg gccctcggag acccagcttc tctgacaagc agattagacc aaaaggctgc
     2641 ctcaaagata tgccactttg aaggaaagcg tagagaagcg tttacataaa agaagacgct
     2701 tcctgttcag tggacaactt catgccactt tcaaggcaca ccgatggcca ggtgggacat
     2761 ttgtactgta gcagcacatg gcaaaggtga gccagaagca gcctggatgc tggctgatcc
     2821 ggaggccttt gtgaagagca aggagagggc tccagcccac ctccccgcag ctctgcccca
     2881 gcccccgtgg gccacaggga ggctcaaggg gagtgaacta ggtaaacaga ttcctggaaa
     2941 ctcacatctg gatgcagctg gaagagttaa atatttacat tggtggcttc cctggaccac
     3001 cgcgaacaca aacatccaca ccacagggct gagttttgtg caaatgatgg ggctttgcat
     3061 tttttattaa cattttcctc tcacgtggtt tacatcaatt tataataatc tacataagtt
     3121 gaaacagaac atagacaaaa aaatatatcc ttaccaactt attaaagtca gatattcatg
     3181 aa
//
