LOCUS       NM_020375               8209 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens TP53 induced glycolysis regulatory phosphatase
            (TIGAR), mRNA.
ACCESSION   NM_020375
VERSION     NM_020375.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Tan,Q.L., Zhang,M.X., Yao,D.H., Yan,Y., Yang,X.F., Qin,Z.H.,
            Gong,Y. and Meng,Q.
  TITLE     TIGAR Protects Against Adenine-Induced Ferroptosis in Human
            Proximal Tubular Epithelial Cells by Activating the mTOR/S6KP70
            Axis
  JOURNAL   Nutr Cancer 75 (6), 1464-1472 (2023)
   PUBMED   37140263
  REMARK    GeneRIF: TIGAR Protects Against Adenine-Induced Ferroptosis in
            Human Proximal Tubular Epithelial Cells by Activating the
            mTOR/S6KP70 Axis.
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Fu,J., Yu,S., Zhao,X., Zhang,C., Shen,L., Liu,Y. and Yu,H.
  TITLE     Inhibition of TIGAR Increases Exogenous p53 and Cisplatin
            Combination Sensitivity in Lung Cancer Cells by Regulating
            Glycolytic Flux
  JOURNAL   Int J Mol Sci 23 (24), 16034 (2022)
   PUBMED   36555672
  REMARK    GeneRIF: Inhibition of TIGAR Increases Exogenous p53 and Cisplatin
            Combination Sensitivity in Lung Cancer Cells by Regulating
            Glycolytic Flux.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8209)
  AUTHORS   Liu,M.Y., Li,H.M., Wang,X.Y., Xia,R., Li,X., Ma,Y.J., Wang,M. and
            Zhang,H.S.
  TITLE     TIGAR drives colorectal cancer ferroptosis resistance through
            ROS/AMPK/SCD1 pathway
  JOURNAL   Free Radic Biol Med 182, 219-231 (2022)
   PUBMED   35271998
  REMARK    GeneRIF: TIGAR drives colorectal cancer ferroptosis resistance
            through ROS/AMPK/SCD1 pathway.
REFERENCE   4  (bases 1 to 8209)
  AUTHORS   Simon-Molas,H., Sanchez-de-Diego,C., Navarro-Sabate,A., Castano,E.,
            Ventura,F., Bartrons,R. and Manzano,A.
  TITLE     The Expression of TP53-Induced Glycolysis and Apoptosis Regulator
            (TIGAR) Can Be Controlled by the Antioxidant Orchestrator NRF2 in
            Human Carcinoma Cells
  JOURNAL   Int J Mol Sci 23 (3), 1905 (2022)
   PUBMED   35163828
  REMARK    GeneRIF: The Expression of TP53-Induced Glycolysis and Apoptosis
            Regulator (TIGAR) Can Be Controlled by the Antioxidant Orchestrator
            NRF2 in Human Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8209)
  AUTHORS   Mondal,P., Gadad,S.S., Adhikari,S., Ramos,E.I., Sen,S., Prasad,P.
            and Das,C.
  TITLE     TCF19 and p53 regulate transcription of TIGAR and SCO2 in HCC for
            mitochondrial energy metabolism and stress adaptation
  JOURNAL   FASEB J 35 (9), e21814 (2021)
   PUBMED   34369624
  REMARK    GeneRIF: TCF19 and p53 regulate transcription of TIGAR and SCO2 in
            HCC for mitochondrial energy metabolism and stress adaptation.
REFERENCE   6  (bases 1 to 8209)
  AUTHORS   Jen,K.Y. and Cheung,V.G.
  TITLE     Identification of novel p53 target genes in ionizing radiation
            response
  JOURNAL   Cancer Res 65 (17), 7666-7673 (2005)
   PUBMED   16140933
REFERENCE   7  (bases 1 to 8209)
  AUTHORS   Katoh,Y. and Katoh,M.
  TITLE     Comparative genomics on mammalian Fgf6-Fgf23 locus
  JOURNAL   Int J Mol Med 16 (2), 355-358 (2005)
   PUBMED   16012775
REFERENCE   8  (bases 1 to 8209)
  CONSRTM   ADHR Consortium
  TITLE     Autosomal dominant hypophosphataemic rickets is associated with
            mutations in FGF23
  JOURNAL   Nat Genet 26 (3), 345-348 (2000)
   PUBMED   11062477
REFERENCE   9  (bases 1 to 8209)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 8209)
  AUTHORS   Loehrer,P.J. Sr., Birch,R., Williams,S.D., Greco,F.A. and
            Einhorn,L.H.
  TITLE     Chemotherapy of metastatic seminoma: the Southeastern Cancer Study
            Group experience
  JOURNAL   J Clin Oncol 5 (8), 1212-1220 (1987)
   PUBMED   2442317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ272206.1, AC008012.8 and
            BX098281.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020375.2.
            
            Summary: This gene is regulated as part of the p53 tumor suppressor
            pathway and encodes a protein with sequence similarity to the
            bisphosphate domain of the glycolytic enzyme that degrades
            fructose-2,6-bisphosphate. The protein functions by blocking
            glycolysis and directing the pathway into the pentose phosphate
            shunt. Expression of this protein also protects cells from DNA
            damaging reactive oxygen species and provides some protection from
            DNA damage-induced apoptosis. The 12p13.32 region that includes
            this gene is paralogous to the 11q13.3 region. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP201817.1, SRR1803614.162694.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000179259.6/ ENSP00000179259.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-899               AJ272206.1         9-907
            900-8198            AC008012.8         144410-151708       c
            8199-8209           BX098281.1         9-19                c
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.32"
     gene            1..8209
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /note="TP53 induced glycolysis regulatory phosphatase"
                     /db_xref="GeneID:57103"
                     /db_xref="HGNC:HGNC:1185"
                     /db_xref="MIM:610775"
     exon            1..91
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..872
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /EC_number="3.1.3.46"
                     /note="TP53-induced glycolysis and apoptosis regulator;
                     transactivated by NS3TP2 protein; probable
                     fructose-2,6-bisphosphatase TIGAR;
                     fructose-2,6-bisphosphate 2-phosphatase"
                     /codon_start=1
                     /product="fructose-2,6-bisphosphatase TIGAR"
                     /protein_id="NP_065108.1"
                     /db_xref="CCDS:CCDS8525.1"
                     /db_xref="GeneID:57103"
                     /db_xref="HGNC:HGNC:1185"
                     /db_xref="MIM:610775"
                     /translation="MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGI
                     FLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSE
                     LRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSN
                     CLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLP
                     ATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR"
     exon            92..129
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     exon            130..251
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     exon            252..329
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     exon            330..440
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     exon            441..8209
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1323..1328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /note="hexamer: AATAAA"
     polyA_site      1357
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /note="major polyA site"
     regulatory      2740..2745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /note="hexamer: AATAAA"
     polyA_site      2774
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
     regulatory      8186..8191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
                     /note="hexamer: ATTAAA"
     polyA_site      8209
                     /gene="TIGAR"
                     /gene_synonym="C12orf5; FR2BP"
ORIGIN      
        1 agcccgcagt gcaggggcag cgcggcgcgg ggccaccgac gggacgcggc tccgggaaca
       61 tggctcgctt cgctctgact gttgtccggc atggagaaac aagatttaac aaggagaaaa
      121 taatccaagg acaaggagta gatgaacctc tttcagaaac tggatttaaa caagcagcag
      181 ctgctggtat atttctgaat aatgtgaagt ttactcatgc tttctccagt gatctcatga
      241 ggacaaagca gaccatgcat ggaattttgg agagaagcaa attttgcaaa gatatgacgg
      301 taaagtatga ctcaagactt cgggaaagga aatacggggt tgtagaaggc aaagcgctaa
      361 gtgagctgag ggccatggcc aaagcagcca gggaagagtg ccctgtgttt acaccgcccg
      421 gaggagagac gctggaccag gtgaaaatgc gtggaataga cttttttgaa tttctttgtc
      481 aactaatcct gaaagaagcg gatcaaaaag aacagttttc ccaaggatct ccaagcaact
      541 gtctggaaac ttctttggca gagatatttc ctttaggaaa aaatcacagc tctaaagtta
      601 attcagacag cggtattcca ggattagcag ccagtgtctt agttgtgagt cacggtgctt
      661 acatgagaag tctgtttgat tattttctga ctgaccttaa gtgttcctta ccagccactc
      721 tgagcagatc tgaacttatg tcagtcactc ccaatacagg gatgagtctc tttatcataa
      781 actttgagga aggaagagaa gttaaaccaa cggttcagtg tatttgtatg aacctacagg
      841 atcatctaaa tggactgact gaaactcgct aaggttaaat ctgcatcaaa atctaaccat
      901 tttgagcctc tgaagggagt gccattggct ttatttactt ctctcctctg ctagttctga
      961 tttggaaaca gttaaaagcc aatttttagc tccagtggaa ccatagccac ataaaacttt
     1021 aatggacaac catatagaat taacttattt tgtccaagta cagttggcat tttccagaat
     1081 aattttacca ccctgctaga tgtcatctct ggattgcaca tggatgatga aggaactcag
     1141 cattgaaagt tgggggatta gtaaccttgt tacaacggtt tctttttcat tttagcctat
     1201 tttaatggct attggtaaga tactgtatgt ttttagtatc tcatccagtg cttagaagaa
     1261 agaatggttt ataattccca gtacatgttt atattgactg tgttatattt ttaaatcctt
     1321 taaataaaaa atccttataa gtttatgtaa agcaaaataa cactaacatg ttttacgtat
     1381 aaatcaacta ttggaatttt ttattctaga gggcttgaaa tgtggatgaa tacagattat
     1441 gattgcagaa tggtctgggg gaagaagtta aaacatttga tttaaataca gtaaagaaag
     1501 aatcttggag cagaagatag aagaacagcc ttttctaaat aatgccttgc caaagtcaag
     1561 tcaatggaaa ccaggaaagc caaaaaaatg aagaatatcc acttgcttct tatctaacct
     1621 gtaggctgaa gaatttccag tttgaggaag gctctaaaga aagtaaacag ttaacagaac
     1681 accacacagt aatcagaata aaagaggggc cttctttgta aagaatggtg ctctgggccg
     1741 ggcccagtgg ctcacacctg taatcccagc actttgggag gctgaggcgg gcggatcaca
     1801 aggtcaggaa ttctagacca gcctggccaa caatggtgaa accctgtctc tactacaaat
     1861 acaaaaaagc caggcgtggt agcaggcgcc tgtaatccca gctacttggg aggttgaggc
     1921 aggagaatcg cttgaacccg ggaggtggag gttgcagtga gccgagatcg caccacttca
     1981 ctccagccta ggtgacagag gggatgacag agggtgctct gcgggcaggg aaggcggggt
     2041 ggttgggggg gagagaatgt tgctctgata accacagctg tggttatttt gtggcaggta
     2101 caggtgaggt tgcctcttct ttccaccaga tggcactgga gaaaagggat tttgattaac
     2161 ttaattttat acacaagatt ggtctttcag aaatctttat ctaaatgaag aaactgttct
     2221 acatgtagtt cgcttttaag tcaccatgtt gctaaatgct gtgagtctca actttaaaga
     2281 gggttaatac taaagttgtc agttaaggct atagtcacat atagtcaaaa tttactttga
     2341 catcatttta aatcatatgt aaaattcagt attatattgt tcttagtgtg tctagcacta
     2401 ccagttttct accagtattg aaacagggca cgtgtttgag catcagagga agtatactgt
     2461 aaataaaaat ttgaaaaata agcaaaaagg acagacaata attatctcca ttcccaccat
     2521 actgaagtca tctttccact ttttttttta aagaaacaaa acataaagat aaaagtagag
     2581 ttctctttaa ccagctctca cagtcctgtt ccctccagag gcagccactg cagtgagtct
     2641 gtgtatatgt tcacctgttc atctgcttgc aatgttcatc tgttccttat attttcacct
     2701 agtgtctgga aggagatggt actgttttgg tgctttaaaa ataaatatca agccatctgt
     2761 gttctttatt tttacttatg tcttggaggc atctctgtgt tgatatatat agatctagtt
     2821 aattataacc actctattct attaaatata gcacattttt gtttattagt gggcatttag
     2881 gtatattcag tttttatttt ctttgctttt tttctttttt tttttttttg gagacagagt
     2941 ctctctctgt tgcccaggct ggggtgcaat ggcgtgatct tggctcactg caacctccac
     3001 ctctcgggtt caagcaattc ttgtgcctca gcctcctgag tagctgggac tacaggcatg
     3061 caccaccacg cctggccttt tttttttttt tagtagagac ggggtttcgc catgttggcc
     3121 aggctggtct tgaacttctg acctcaggtg atccacctgc cttggccttc caaagtgctg
     3181 ggattgcagg cataagccac cgtgcctggc ctatgatgca tttttttaat atgtgttctt
     3241 aatttttaag acaaaccaat ataccttatc ctttatgggg tttttttggt gtgtatcttt
     3301 aagaagactt ctctaccatg aggtcataca gtaatgtctt cttacagttt ttgagaactt
     3361 ctgaatgtca aatcttgaaa ttgaaatttg ctttttatag tataaggtaa gaatttaatt
     3421 ttttttttca tgtggttgaa acagttatcc caacaccatt gtaaaggctg ttctttccca
     3481 attggtttgt ggtgtgtgct ctcaggcctc acaccccctc atttgctaga gtctgtggac
     3541 tcttttagtt atctgtgtat ttccttatca gtgtcatact tagttactgt ggctttgcag
     3601 ctgactgtct taaagggtgg atacctcctc ttgttcattt ttaaagttgt cttaactatt
     3661 tatgcacttt tattcttcca tataaatttt ggaatcaatt ggtcaatttc cagaaaaatt
     3721 ctgttacaaa ttttattgga gtcacatgca tctcttcatc tattcaatac gtattttttg
     3781 agcatggcac acacatgcca ggctatttta agaactacta caactatgat aaagctgtga
     3841 atatgtagcc atgaaccaaa acaaagtctc tgtccttgtg gaacatttgt tctgtcagag
     3901 aagacagtgt gttggctcac attgtggtca gtgctgttga gcaaaatagg tcagagtaag
     3961 ggggatggag actggtggga ggaatgctgc tttatccagg atgggcaggg aggactcgat
     4021 ggtgtcagca ctgaaggatg taagatctgc tgctctgggg agaggagcag catggaagga
     4081 gtagagtgca gaggccatga ggaaggatca ggcttgactc ctttgagcaa gggggatggg
     4141 agagtgacgg gagaagagga caggccacat ggcctggtgg cctgtgctga ggccttgggc
     4201 ttttactcaa gtgagatgag atgccattgg ccagtttggg cagtgatgtg atcagacttg
     4261 tttcagcagg accatcctgc ttgcaatgtg gagagcaggc tgtagggggt gctataggag
     4321 cacaaggttg gaggcaggga agatggcagc actggctgtt gggccatttg aagaagcaag
     4381 gccagggatg ttggcaggga ccaggccaga gtgcctccaa tggctaacta aggagcctgg
     4441 acattgaatt tctaggttag cttaggattt ctcatcctcc catctgtgaa tgtcatagat
     4501 ctctcatttt gcctgagtct tttatgcaat gatattttat ttgtaatatt ttcccggttt
     4561 ttgattgttt tttaacaaat gaatttttta agagacttaa ttttttaaga gaaactttaa
     4621 tttcccagca aaagtgagag gaaggtagag agatttccca tctatcccct gcccctacac
     4681 acgcattgct tctcctacta tcaacatccc ccaccagacc agtgcatctg ttaccatcgg
     4741 tgagcccaca ttacactgac atatccacat cacctgaaac ccatagttta cattagggtt
     4801 cattcttggt gttgcatgat ctgtgggttt ggacaaacgt atgaagacat gtattccacc
     4861 attatagtat catgcagagt attttcactg ccctaaaagt cctctgtgtt ccctgtactc
     4921 atctttccca ccttgtcttg cctgtttctg ttaggtttag tccgaggcct agcagaggga
     4981 atgggcagaa gtttccagtt ccaggcttca ccccaggctc gcagcttcaa gcagagcctg
     5041 tcgcttaggc tcccttgcac ctggagtcga aaccatggct cccattcacc tgtctcttat
     5101 tttcctgtcc ttcccactcc tcactcacct actattcatt tccatttttg gatgaggatt
     5161 ctcctttatt gctttttaac taaaaacctg ttttagttag catctttatt ctaatttctt
     5221 taaatgctat gtcatactca gcaaggacat gctcatatta ggggaagcac ataaagtaag
     5281 atatccctga gagaacagtg catctcacac ccattccatg gcttaggctt atgcaggaag
     5341 tgcaatattt ggccatatca cttaagtaca tatgcttata ttatgtatac ttgcatgcat
     5401 attacattta caagtagatg aattcgtgta agttaaatgg ttctattata tgtcttcact
     5461 atattaaggt cagtcacaat ttaattgaaa tagatcatcc taggtgaatc cacctggcat
     5521 aattatcagc cctgttaagc acagacacag gagatgcaaa ggaaagaagt attcagtaga
     5581 gcggtggtgc tgggctgagt acctgctatc agacgcagca cttaccagtt tggaaattga
     5641 atggggctcc tagaaaacat ccttgtttca tgtaaatgtt gctttttacc cctctttctt
     5701 tgggccccat caatttggaa gtacagcttt cagattgaag tatgtgtcag ttcctttgtg
     5761 tccaaacaag atggctaagt aggtcgtcat gacaggtaac taccagtgct ttaaaaactt
     5821 taggatttcc ccccacagtt ctcaatcact tggttcattg ttcagcaata tggcctaagg
     5881 gggtgatttc ttcattgata agtgacaggg cagggacact ggaaagatct attgtcagaa
     5941 ggcctggatt caggttcaag gcctggttct tagtaatttt gtgaccaagc aatcgctgag
     6001 gtggctgggc gtggtggctc gcacctgtaa tcccagcact ttgggaggcc aaggcgggca
     6061 gatcacgagg tcaggagata gagaccatcc tggctaacac agtgaaacct cgtctccact
     6121 gaaaaataga aaaaattagc caggcctggt ggccggcgcc tgtagtccca gctacttggg
     6181 aggctgaggc agtagaatgg cgtgaacccg ggaggtggag cttgcagtga gccaaggtcg
     6241 cgccactgca ctgcagcctg ggtgacagag caagactggg tctcaaaaaa aaaaaaaaaa
     6301 aatcatttga taaataatgg gctgtgcatg gtgactcagg cctgtaatcc cagcactttg
     6361 ggaggccaag gtgggtggat tacctgaggt caggagttca agaccagcct ggccaacatg
     6421 gtgaaaccct gtctttacta aaaatacaaa aattagctgg gcgtctccaa aaaaaaaaaa
     6481 aaagaaaaag aaaaatcact gagctactgt atcctcatag agaataatta cagtaatgtg
     6541 tatgtgcgtg tgttttggta atcttttatc ttggatttat caaacctaca gaaaagttgc
     6601 aagaacagta gaaggaatgg ctgtatactc ttcacactta ttcaccactt gcttacctgt
     6661 tgtcccactt gctttgccat tttttctctc tccctctgtt ttcccctgaa acattggaga
     6721 gtaactttga gtcctcatac tcttccccta taaaatttca atgtgtgttt cctgagatca
     6781 acaatgttct tatatataac ccagaataaa tatctgagtc aggaaatttg acattgatat
     6841 tgcctattag ctaatcctta gaccaaattt aaattctgtc aattattttg ttgatgtacc
     6901 tgtggctttt tttttttttc tgaatcaaga atccaatcaa tccaggactg tgtgttgtgt
     6961 tgcatttagt tgtcatgttt cttcatgtcc ttccgtctac tcctttgcct tcctttgccc
     7021 ttcttttttt ctcccaccat ctatctaacc agatgaactt tgtctttctt gatcttgtta
     7081 ctttttaaaa aataggtgag ttcttgtggt tttttttcct gacatatcct cattattaga
     7141 tttggattgt atacttttgt cagaagcagc ccagaaagga tgtgtccttg gtcatgtcag
     7201 gagggacagg acgttgtatc aatatgtctg gttcctcatt aaatttgcaa ctactctttt
     7261 agcctttatt gtttattttc tgactctgtt ttttttttct ttagccaact cacgccatga
     7321 ctctactttt gcttctatgt ttattagttg gcattctgta ctaagacagc tttacccttc
     7381 ttgcctactt atttatgtca gtatgagctc agaagttctt attcaggggc ttataataca
     7441 gtactcttat tattttatgg ttacacagtc ccaactttgt cctgtgggaa ccctttcagg
     7501 ctggctctta tgttctggtg acataatctc atccttcttt aagtactttc tttctggtat
     7561 taacaatatg ctccagactc attcttcccg agccccagcc ccagaatcag ctttttctct
     7621 aaagagccct ggttctttgg aaatgaaatg aaagacgcct ggtgtgcatg gtatttagaa
     7681 accaagatct cggtgccagg gtgctcattg ctaatagtgc ctttaaaaaa attactgcat
     7741 tgaattccaa tgcatagata cacaacagtt tgtttattct tccattgacg gaacacaagg
     7801 accatttcta gtttggagct attatgaata aagctgctat aaatattttt acaagtcttt
     7861 ttgcggacgt atgttttcat ttctcttggg taaataatct gggagtggaa ttgctgagtt
     7921 ataggttata tgttggtgta tgtttagttt tataagacac ttgccagatc tttttccatg
     7981 gtggttgtac catttcatgc ttatcaacaa tgagttctag gtgcttcaca tccttgccaa
     8041 catttgttat tatcagtttc taaaatttta gccattctga ttgttgtatg gtagtatatc
     8101 cctgcaattt taactggtga tatggaatgc tttttaatgt acaattctta ttgatcattc
     8161 atacagcttt ctttgtgaaa tgtgtattaa aatcttatcc attaaaaaa
//
