LOCUS       NM_012454               6094 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens TIAM Rac1 associated GEF 2 (TIAM2), transcript variant
            1, mRNA.
ACCESSION   NM_012454
VERSION     NM_012454.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Arunachalam,V., Lea,R., Hoy,W., Lee,S., Mott,S., Savige,J.,
            Mathews,J.D., McMorran,B.J. and Nagaraj,S.H.
  TITLE     Novel genetic markers for chronic kidney disease in a
            geographically isolated population of Indigenous Australians:
            Individual and multiple phenotype genome-wide association study
  JOURNAL   Genome Med 16 (1), 29 (2024)
   PUBMED   38347632
  REMARK    GeneRIF: Novel genetic markers for chronic kidney disease in a
            geographically isolated population of Indigenous Australians:
            Individual and multiple phenotype genome-wide association study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Chu,C.H., Chen,J.S., Chan,Y.L., Lu,W.J., Huang,Y.T., Mao,P.C.,
            Sze,C.I. and Sun,H.S.
  TITLE     TIAM2S-positive microglia enhance inflammation and neurotoxicity
            through soluble ICAM-1-mediated immune priming
  JOURNAL   FASEB J 37 (11), e23242 (2023)
   PUBMED   37801065
  REMARK    GeneRIF: TIAM2S-positive microglia enhance inflammation and
            neurotoxicity through soluble ICAM-1-mediated immune priming.
REFERENCE   3  (bases 1 to 6094)
  AUTHORS   Cooke,M., Kreider-Letterman,G., Baker,M.J., Zhang,S.,
            Sullivan,N.T., Eruslanov,E., Abba,M.C., Goicoechea,S.M.,
            Garcia-Mata,R. and Kazanietz,M.G.
  TITLE     FARP1, ARHGEF39, and TIAM2 are essential receptor tyrosine kinase
            effectors for Rac1-dependent cell motility in human lung
            adenocarcinoma
  JOURNAL   Cell Rep 37 (5), 109905 (2021)
   PUBMED   34731623
  REMARK    GeneRIF: FARP1, ARHGEF39, and TIAM2 are essential receptor tyrosine
            kinase effectors for Rac1-dependent cell motility in human lung
            adenocarcinoma.
REFERENCE   4  (bases 1 to 6094)
  AUTHORS   Jiang,B., Zhou,L., Lu,J., Wang,Y., Liu,C., Zhou,W. and Guo,J.
  TITLE     Elevated TIAM2 expression promotes tumor progression and is
            associated with unfavorable prognosis in pancreatic cancer
  JOURNAL   Scand J Gastroenterol 56 (1), 59-67 (2021)
   PUBMED   33284659
  REMARK    GeneRIF: Elevated TIAM2 expression promotes tumor progression and
            is associated with unfavorable prognosis in pancreatic cancer.
REFERENCE   5  (bases 1 to 6094)
  AUTHORS   Woroniuk,A., Porter,A., White,G., Newman,D.T., Diamantopoulou,Z.,
            Waring,T., Rooney,C., Strathdee,D., Marston,D.J., Hahn,K.M.,
            Sansom,O.J., Zech,T. and Malliri,A.
  TITLE     STEF/TIAM2-mediated Rac1 activity at the nuclear envelope regulates
            the perinuclear actin cap
  JOURNAL   Nat Commun 9 (1), 2124 (2018)
   PUBMED   29844364
  REMARK    GeneRIF: The authors show that the Rac1 selective guanine
            nucleotide exchange factor Sif and TIAM1-like Exchange Factor STEF,
            also known as TIAM2, localises to the nuclear envelope,
            co-localises with key perinuclear proteins and regulates the
            activity of perinuclear Rac1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6094)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   7  (bases 1 to 6094)
  AUTHORS   Yoshizawa,M., Hoshino,M., Sone,M. and Nabeshima,Y.
  TITLE     Expression of stef, an activator of Rac1, correlates with the
            stages of neuronal morphological development in the mouse brain
  JOURNAL   Mech Dev 113 (1), 65-68 (2002)
   PUBMED   11900975
  REMARK    GeneRIF: stef gene expressed in a stage- and region-specific manner
            in the mouse brain
REFERENCE   8  (bases 1 to 6094)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   9  (bases 1 to 6094)
  AUTHORS   Chiu,C.Y., Leng,S., Martin,K.A., Kim,E., Gorman,S. and Duhl,D.M.
  TITLE     Cloning and characterization of T-cell lymphoma invasion and
            metastasis 2 (TIAM2), a novel guanine nucleotide exchange factor
            related to TIAM1
  JOURNAL   Genomics 61 (1), 66-73 (1999)
   PUBMED   10512681
  REMARK    Erratum:[Genomics 2000 Feb 1;63(3):439]
REFERENCE   10 (bases 1 to 6094)
  AUTHORS   Hoshino,M., Sone,M., Fukata,M., Kuroda,S., Kaibuchi,K.,
            Nabeshima,Y. and Hama,C.
  TITLE     Identification of the stef gene that encodes a novel guanine
            nucleotide exchange factor specific for Rac1
  JOURNAL   J Biol Chem 274 (25), 17837-17844 (1999)
   PUBMED   10364228
  REMARK    GeneRIF: STEF protein is a guanine nucleotide exchange factor that
            specifically activates Rac1 in vitro and in cultured cells, which
            may lead to changes in the actin cytoskeletal network.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL136228.8, AL355343.18,
            AL596202.5 and AL139101.13.
            
            On Jun 20, 2020 this sequence version replaced NM_012454.3.
            
            Summary: This gene encodes a guanine nucleotide exchange factor. A
            highly similar mouse protein specifically activates ras-related C3
            botulinum substrate 1, converting this Rho-like guanosine
            triphosphatase (GTPase) from a guanosine diphosphate-bound inactive
            state to a guanosine triphosphate-bound active state. The encoded
            protein may play a role in neural cell development. Alternatively
            spliced transcript variants encoding different isoforms have been
            described. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.24698.1,
                                           SRR11853561.25535.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144835
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000682666.1/ ENSP00000507157.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               AL136228.8         68408-68585
            179-269             AL355343.18        35961-36051
            270-380             AL355343.18        73162-73272
            381-1580            AL355343.18        74890-76089
            1581-2016           AL355343.18        82849-83284
            2017-2189           AL355343.18        90278-90450
            2190-2414           AL355343.18        93782-94006
            2415-2600           AL355343.18        110087-110272
            2601-2747           AL355343.18        110935-111081
            2748-2909           AL355343.18        122488-122649
            2910-3014           AL355343.18        124711-124815
            3015-3093           AL355343.18        125050-125128
            3094-3186           AL355343.18        127898-127990
            3187-3450           AL355343.18        128909-129172
            3451-3554           AL355343.18        156876-156979
            3555-3734           AL596202.5         8940-9119
            3735-3803           AL596202.5         12421-12489
            3804-3929           AL596202.5         13068-13193
            3930-4038           AL596202.5         14033-14141
            4039-4218           AL596202.5         16410-16589
            4219-4337           AL596202.5         18261-18379
            4338-4446           AL596202.5         19323-19431
            4447-4505           AL596202.5         20355-20413
            4506-4611           AL596202.5         21358-21463
            4612-4699           AL596202.5         22383-22470
            4700-4854           AL596202.5         22829-22983
            4855-6094           AL139101.13        612-1851
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.2-q25.3"
     gene            1..6094
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="TIAM Rac1 associated GEF 2"
                     /db_xref="GeneID:26230"
                     /db_xref="HGNC:HGNC:11806"
                     /db_xref="MIM:604709"
     exon            1..178
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            179..269
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            270..380
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            381..1580
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             387..5492
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="isoform a is encoded by transcript variant 1;
                     T-lymphoma invasion and metastasis-inducing protein 2; SIF
                     and TIAM1-like exchange factor; T cell lymphoma invasion
                     and metastasis 2; rho guanine nucleotide exchange factor
                     TIAM2"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor TIAM2
                     isoform a"
                     /protein_id="NP_036586.3"
                     /db_xref="CCDS:CCDS34558.1"
                     /db_xref="GeneID:26230"
                     /db_xref="HGNC:HGNC:11806"
                     /db_xref="MIM:604709"
                     /translation="MGNSDSQYTLQGSKNHSNTITGAKQIPCSLKIRGIHAKEEKSLH
                     GWGHGSNGAGYKSRSLARSCLSHFKSNQPYASRLGGPTCKVSRGVAYSTHRTNAPGKD
                     FQGISAAFSTENGFHSVGHELADNHITSRDCNGHLLNCYGRNESIASTPPGEDRKSPR
                     VLIKTLGKLDGCLRVEFHNGGNPSKVPAEDCSEPVQLLRYSPTLASETSPVPEARRGS
                     SADSLPSHRPSPTDSRLRSSKGSSLSSESSWYDSPWGNAGELSEAEGSFLAPGMPDPS
                     LHASFPPGDAKKPFNQSSSLSSLRELYKDANLGSLSPSGIRLSDEYMGTHASLSNRVS
                     FASDIDVPSRVAHGDPIQYSSFTLPCRKPKAFVEDTAKKDSLKARMRRISDWTGSLSR
                     KKRKLQEPRSKEGSDYFDSRSDGLNTDVQGSSQASAFLWSGGSTQILSQRSESTHAIG
                     SDPLRQNIYENFMRELEMSRTNTENIETSTETAESSSESLSSLEQLDLLFEKEQGVVR
                     KAGWLFFKPLVTVQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSMDQSSAPRC
                     ALFAEDSIVQSVPEHPKKENVFCLSNSFGDVYLFQATSQTDLENWVTAVHSACASLFA
                     KKHGKEDTLRLLKNQTKNLLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIQQWEQ
                     NLEKFHMDLFRMRCYLASLQGGELPNPKSLLAAASRPSKLALGRLGILSVSSFHALVC
                     SRDDSALRKRTLSLTQRGRNKKGIFSSLKGLDTLARKGKEKRPSITQVDELLHIYGST
                     VDGVPRDNAWEIQTYVHFQDNHGVTVGIKPEHRVEDILTLACKMRQLEPSHYGLQLRK
                     LVDDNVEYCIPAPYEYMQQQVYDEIEVFPLNVYDVQLTKTGSVCDFGFAVTAQVDERQ
                     HLSRIFISDVLPDGLAYGEGLRKGNEIMTLNGEAVSDLDLKQMEALFSEKSVGLTLIA
                     RPPDTKATLCTSWSDSDLFSRDQKSLLPPPNQSQLLEEFLDNFKKNTANDFSNVPDIT
                     TGLKRSQTDGTLDQVSHREKMEQTFRSAEQITALCRSFNDSQANGMEGPRENQDPPPR
                     SLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEMESLFGS
                     LPEMLEFQKVFLETLEDGISASSDFNTLETPSQFRKLLFSLGGSFLYYADHFKLYSGF
                     CANHIKVQKVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLKEL
                     VSLTDQESEEHYHLTEALKAMEKVASHINEMQKIYEDYGTVFDQLVAEQSGTEKEVTE
                     LSMGELLMHSTVSWLNPFLSLGKARKDLELTVFVFKRAVILVYKENCKLKKKLPSNSR
                     PAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLC
                     CSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRS
                     LKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHP
                     GLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQ
                     PGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATI
                     DLNSVLEREFSVQSLTSVVSEECFYETESHGKS"
     exon            1581..2016
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2189
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2414
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2600
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2747
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2748..2909
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3014
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3093
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3186
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3187..3450
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3554
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3734
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3735..3803
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3929
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3930..4038
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4039..4218
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4337
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4338..4446
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4447..4505
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4611
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4612..4699
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4700..4854
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4855..6094
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6011..6016
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="hexamer: ATTAAA"
     polyA_site      6033
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="major polyA site"
     regulatory      6073..6078
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="hexamer: AGTAAA"
     polyA_site      6094
                     /gene="TIAM2"
                     /gene_synonym="STEF; TIAM-2"
                     /note="major polyA site"
ORIGIN      
        1 agcggtaacc gtaactgtgg ccgcggccgc cgcaggcgca cagcgcgcgt ccaagtggag
       61 aacaaagtga cccccagaac ttctccaact cctcccggcg ttcccgcgag ggccacgcgc
      121 cgagggtagc ggagggcggc gcgcgaccgg ccccaccgag cccggcgcgc gacccgaggg
      181 ctctgtgtat gaatgacaag gataccttca gccagctcat tctggatgaa tgaatgatta
      241 cactaagtgt cctccacatt cctctgtggg ctcacttcat ggactcactt tgcgtgcttg
      301 ttaaatgtgc tgtgttgctc ccaagaccat gtaaagccta ctgaccacta acctccctca
      361 cagcagaaac tagacgtcag gttaaaatgg gcaactccga cagtcagtac acccttcaag
      421 gatctaaaaa tcatagcaat actattactg gtgctaagca aattccttgc tccctgaaaa
      481 tacgtggcat tcatgcaaaa gaggaaaagt cattgcatgg atggggtcac ggaagcaacg
      541 gagcaggtta caagtccagg tccctggccc gaagctgcct ttctcacttt aagagtaacc
      601 agccttacgc atcgagactc ggtggcccca catgcaaggt ctccagaggt gttgcctact
      661 ccacgcacag gacaaatgcc ccagggaagg atttccaggg catcagtgct gctttctcaa
      721 ctgagaatgg cttccactct gttggccacg agctggcaga taaccacatc acctccagag
      781 actgcaacgg acaccttctc aactgctacg ggaggaatga gagcattgcc tccaccccac
      841 cgggcgaaga ccgcaagagc ccccgagtgc tcatcaaaac gctggggaag ctggatgggt
      901 gtttaagggt cgagttccac aatggtggca accccagcaa agtgcctgca gaggactgca
      961 gtgagccggt gcagctgctg aggtactcac ctaccttagc atcggaaacc tcccctgtgc
     1021 ctgaagccag gagggggtcc agcgccgatt ccctgcccag ccatcgcccc tctcccacgg
     1081 actctcgcct gcggtccagc aaaggcagct ccctgagttc tgagtcatcc tggtacgact
     1141 ccccttgggg caatgctgga gagctgagcg aggctgaggg ctccttcctg gcccccggca
     1201 tgcctgaccc cagtctccat gccagcttcc cacctggcga tgccaaaaag cctttcaacc
     1261 aaagctcttc cctctcctcc ctccgggaac tgtacaaaga tgccaacctg gggagcctct
     1321 ccccctcagg tatccgcctt tctgatgaat acatgggcac gcatgccagc ctgagcaacc
     1381 gtgtctcttt tgcttccgac attgatgtgc cctccagagt ggcacacggg gaccccatcc
     1441 agtacagttc cttcactctc ccctgtcgga agcccaaagc ctttgttgag gatactgcga
     1501 agaaggactc cctcaaagcc aggatgcgac ggatcagtga ctggacggga agcctctcaa
     1561 ggaagaaaag gaaactccag gagccgaggt ccaaggaggg cagtgactac tttgacagtc
     1621 gctctgatgg actgaataca gatgtgcagg gatcctccca ggcatctgct tttctgtggt
     1681 cagggggctc tactcagatc ctgtctcaga gaagtgaatc cacacatgcg attggcagcg
     1741 atcccctccg gcagaacatt tatgagaatt tcatgcgaga gttggaaatg agcaggacca
     1801 acactgagaa catagaaaca tctacagaaa ccgccgagtc cagcagcgag tcactcagct
     1861 ctctggaaca gctggatctg ctctttgaga aggaacaggg ggtggtccgg aaggccgggt
     1921 ggctcttctt caagcccctg gtcactgtgc agaaggaaag gaagcttgag ctggtggcac
     1981 gaaggaaatg gaaacagtac tgggtaacgc tgaaaggatg cacgctgctg ttttatgaga
     2041 cctatgggaa gaattccatg gatcagagca gtgcccctcg gtgtgctctg tttgcagaag
     2101 acagcatagt gcagtctgtt ccagagcatc ccaagaaaga aaatgtgttc tgcctcagca
     2161 actcctttgg agatgtctac cttttccagg ccaccagcca gacagatcta gaaaactggg
     2221 tcactgctgt acactctgct tgtgcatccc tttttgcaaa gaagcatggg aaagaggaca
     2281 cgctgcggct gctgaagaac cagaccaaaa acctgcttca gaagatagac atggacagca
     2341 agatgaagaa gatggcagag ctgcagctgt ccgtggtgag cgacccaaag aacaggaaag
     2401 ccatagagaa ccagatccag caatgggagc agaatcttga gaaatttcac atggatctgt
     2461 tcaggatgcg ctgctatctg gccagcctac aaggtgggga gttaccgaac ccaaagagtc
     2521 tccttgcagc cgccagccgc ccctccaagc tggccctcgg caggctgggc atcttgtctg
     2581 tttcctcttt ccatgctctg gtatgttcta gagatgactc tgctctccgg aaaaggacac
     2641 tgtcactgac ccagcgaggg agaaacaaga agggaatatt ttcttcgtta aaagggctgg
     2701 acacactggc cagaaaaggc aaggagaaga gaccttctat aactcaggtc gatgaacttc
     2761 tgcatatata tggttcaaca gtagacggtg ttccccgaga caatgcatgg gaaatccaga
     2821 cttatgtcca ctttcaggac aatcacggag ttactgtagg gatcaagcca gagcacagag
     2881 tagaagatat tttgactttg gcatgcaaga tgaggcagtt ggaacccagc cattatggcc
     2941 tacagcttcg aaaattagta gatgacaatg ttgagtattg catccctgca ccatatgaat
     3001 atatgcaaca acaggtttat gatgaaatag aagtctttcc actaaatgtt tatgacgtgc
     3061 agctcacgaa gactgggagt gtgtgtgact ttgggtttgc agttacagcg caggtggatg
     3121 agcgtcagca tctcagccgg atatttataa gcgacgttct tcccgatggc ctggcgtatg
     3181 gggaagggct gagaaagggc aatgagatca tgaccttaaa tggggaagct gtgtctgatc
     3241 ttgaccttaa gcagatggag gccctgtttt ctgagaagag cgtcggactc actctgattg
     3301 cccggcctcc ggacacaaaa gcaaccctgt gtacatcctg gtcagacagt gacctgttct
     3361 ccagggacca gaagagtctg ctgccccctc ctaaccagtc ccaactgctg gaggaattcc
     3421 tggataactt taaaaagaat acagccaatg atttcagcaa cgtccctgat atcacaacag
     3481 gtctgaaaag gagtcagaca gatggcactc tggatcaggt ttcccacagg gagaaaatgg
     3541 agcagacatt caggagtgct gagcagatca ctgcactgtg caggagtttt aacgacagtc
     3601 aggccaacgg catggaagga ccgcgggaga atcaggatcc tcctccgagg tctctggccc
     3661 gccacctgtc tgatgcagac cgcctccgca aagtcatcca ggagcttgtg gacacagaga
     3721 agtcctacgt gaaggatttg agctgcctct ttgaattata cttggagcca cttcagaatg
     3781 agacctttct tacccaagat gagatggagt cactttttgg aagtttgcca gagatgcttg
     3841 agtttcagaa ggtgtttctg gagaccctgg aggatgggat ttcagcatca tctgacttta
     3901 acaccctaga aaccccctca cagtttagaa aattactgtt ttcccttgga ggctctttcc
     3961 tttattacgc ggaccacttt aaactgtaca gtggattctg tgctaaccat atcaaagtac
     4021 agaaggttct ggagcgagct aaaactgaca aagccttcaa ggcttttctg gacgcccgga
     4081 accccaccaa gcagcattcc tccacgctgg agtcctacct catcaagccg gttcagagag
     4141 tgctcaagta cccgctgctg ctcaaggagc tggtgtccct gacggaccag gagagcgagg
     4201 agcactacca cctgacggaa gcactaaagg caatggagaa agtagcgagc cacatcaatg
     4261 agatgcagaa gatctatgag gattatggga ccgtgtttga ccagctagta gctgagcaga
     4321 gcggaacaga gaaggaggta acagaacttt cgatgggaga gcttctgatg cactctacgg
     4381 tttcctggtt gaatccattt ctgtctctag gaaaagctag aaaggacctt gagctcacag
     4441 tatttgtttt taagagagcc gtcatactgg tttataaaga aaactgcaaa ctgaaaaaga
     4501 aattgccctc gaattcccgg cctgcacaca actctactga cttggaccca tttaaattcc
     4561 gctggttgat ccccatctcc gcgcttcaag tcagactggg gaatccagca gggacagaaa
     4621 ataattccat atgggaactg atccatacga agtcagaaat agaaggacgg ccagaaacca
     4681 tctttcagtt gtgttgcagt gacagtgaaa gcaaaaccaa cattgttaag gtgattcgtt
     4741 ctattctgag ggagaacttc aggcgtcaca taaagtgtga attaccactg gagaaaacgt
     4801 gtaaggatcg cctggtacct cttaagaacc gagttcctgt ttcggccaaa ttagcttcat
     4861 ccaggtcttt aaaagtcctg aagaattcct ccagcaacga gtggaccggt gagactggca
     4921 agggaacctt gctggactct gacgagggca gcttgagcag cggcacccag agcagcggct
     4981 gccccacggc tgagggcagg caggactcca agagcacttc tcccgggaaa tacccacacc
     5041 ccggcttggc agattttgcc gacaatctca tcaaagagag tgacatcctg agcgatgaag
     5101 atgatgacca ccgtcagact gtgaagcagg gcagccctac taaagacatc gaaattcagt
     5161 tccagagact gaggatttcc gaggacccag acgttcaccc cgaggctgag cagcagcctg
     5221 gcccggagtc gggtgagggt cagaaaggag gagagcagcc caaactggtc cgggggcact
     5281 tctgccccat taaacgaaaa gccaacagca ccaagaggga cagaggaact ttgctcaagg
     5341 cgcagatccg tcaccagtcc cttgacagtc agtctgaaaa tgccaccatc gacctaaatt
     5401 ctgttctaga gcgagaattc agtgtccaga gtttaacatc tgttgtcagt gaggagtgtt
     5461 tttatgaaac agagagccac ggaaaatcat agtatgattc aatccagata tgggttaaat
     5521 tcctcatttt acttttaaac tggtggtaaa gtggaaattg caaaaaaaaa aaaaaaaaaa
     5581 aactgttcat tcctgggttt tgtgcagtat acattttccc acaaaatggt tgtaaagatt
     5641 taagttattt taatttattg tggatcagaa acctagatga aactggtcag aatctgtaaa
     5701 ttacttagtt tatatccact ttgagcaggt atcaaatgat ttaggatcct taaaattaca
     5761 ttctaataat taagttatgt ggaaaaagta aggctgggga agtcgtgatt aatagttttc
     5821 aaagggccat tttttaaaat cctctgggca ttttctttca gctgtttgtt agtttttgct
     5881 ttatttaaag catatttaag ttattttaat gtggtttagg ggcaaaatgt gcagatactt
     5941 catttttgta agatagattg taatagatgc tgtttatact aaacatgtca taactatcta
     6001 tacagtatat attaaaagaa agcttgtact gtatcttatt tgatgatatt tattttctct
     6061 gccaagctgt atagtaaaag gaaaataagt caca
//
