LOCUS       NM_001353694            7218 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens TIAM Rac1 associated GEF 1 (TIAM1), transcript variant
            12, mRNA.
ACCESSION   NM_001353694 XM_005261037
VERSION     NM_001353694.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Liu,F., Zhang,T., Sun,X., Liu,Z., Xu,W., Dai,X. and Zhang,X.
  TITLE     Deficiency in SPOP-mediated ubiquitination and degradation of TIAM1
            promotes gastric cancer progression
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (3), 167032 (2024)
   PUBMED   38246227
  REMARK    GeneRIF: Deficiency in SPOP-mediated ubiquitination and degradation
            of TIAM1 promotes gastric cancer progression.
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Li,X., Liu,B., Wang,X., Zhuang,Y., Gao,T. and Sun,N.
  TITLE     Tiam1 regulates proliferation, invasion, and differentiation in
            neuroblastoma through the Tiam1/Rac1 signaling pathway
  JOURNAL   Neoplasma 71 (1), 37-47 (2024)
   PUBMED   38506031
  REMARK    GeneRIF: Tiam1 regulates proliferation, invasion, and
            differentiation in neuroblastoma through the Tiam1/Rac1 signaling
            pathway.
REFERENCE   3  (bases 1 to 7218)
  AUTHORS   Ginn,L., Maltas,J., Baker,M.J., Chaturvedi,A., Wilson,L.,
            Guilbert,R., Amaral,F.M.R., Priest,L., Mole,H., Blackhall,F.,
            Diamantopoulou,Z., Somervaille,T.C.P., Hurlstone,A. and Malliri,A.
  TITLE     A TIAM1-TRIM28 complex mediates epigenetic silencing of
            protocadherins to promote migration of lung cancer cells
  JOURNAL   Proc Natl Acad Sci U S A 120 (40), e2300489120 (2023)
   PUBMED   37748077
  REMARK    GeneRIF: A TIAM1-TRIM28 complex mediates epigenetic silencing of
            protocadherins to promote migration of lung cancer cells.
REFERENCE   4  (bases 1 to 7218)
  AUTHORS   Yin,J., Wan,Y., Wang,J. and Xue,M.
  TITLE     Upregulation of miR-22 alleviates oxygen-glucose
            deprivation/reperfusion-induced injury by targeting Tiam1 in
            SH-SY5Y cells
  JOURNAL   Acta Biochim Pol 70 (3), 495-501 (2023)
   PUBMED   37672720
  REMARK    GeneRIF: Upregulation of miR-22 alleviates oxygen-glucose
            deprivation/reperfusion-induced injury by targeting Tiam1 in
            SH-SY5Y cells.
REFERENCE   5  (bases 1 to 7218)
  AUTHORS   Hsu,G.C., Wang,Y., Lu,A.Z., Gomez-Salazar,M.A., Xu,J., Li,D.,
            Meyers,C., Negri,S., Wangsiricharoen,S., Broderick,K., Peault,B.,
            Morris,C. and James,A.W.
  TITLE     TIAM1 acts as an actin organization regulator to control adipose
            tissue-derived pericyte cell fate
  JOURNAL   JCI Insight 8 (13), e159141 (2023)
   PUBMED   37219951
  REMARK    GeneRIF: TIAM1 acts as an actin organization regulator to control
            adipose tissue-derived pericyte cell fate.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7218)
  AUTHORS   Michiels,F., Stam,J.C., Hordijk,P.L., van der Kammen,R.A.,
            Ruuls-Van Stalle,L., Feltkamp,C.A. and Collard,J.G.
  TITLE     Regulated membrane localization of Tiam1, mediated by the
            NH2-terminal pleckstrin homology domain, is required for
            Rac-dependent membrane ruffling and C-Jun NH2-terminal kinase
            activation
  JOURNAL   J Cell Biol 137 (2), 387-398 (1997)
   PUBMED   9128250
REFERENCE   7  (bases 1 to 7218)
  AUTHORS   Chen,H. and Antonarakis,S.E.
  TITLE     Localization of a human homolog of the mouse Tiam-1 gene to
            chromosome 21q22.1
  JOURNAL   Genomics 30 (1), 123-127 (1995)
   PUBMED   8595894
REFERENCE   8  (bases 1 to 7218)
  AUTHORS   Michiels,F., Habets,G.G., Stam,J.C., van der Kammen,R.A. and
            Collard,J.G.
  TITLE     A role for Rac in Tiam1-induced membrane ruffling and invasion
  JOURNAL   Nature 375 (6529), 338-340 (1995)
   PUBMED   7753201
REFERENCE   9  (bases 1 to 7218)
  AUTHORS   Habets,G.G., van der Kammen,R.A., Stam,J.C., Michiels,F. and
            Collard,J.G.
  TITLE     Sequence of the human invasion-inducing TIAM1 gene, its
            conservation in evolution and its expression in tumor cell lines of
            different tissue origin
  JOURNAL   Oncogene 10 (7), 1371-1376 (1995)
   PUBMED   7731688
REFERENCE   10 (bases 1 to 7218)
  AUTHORS   Habets,G.G., van der Kammen,R.A., Jenkins,N.A., Gilbert,D.J.,
            Copeland,N.G., Hagemeijer,A. and Collard,J.G.
  TITLE     The invasion-inducing TIAM1 gene maps to human chromosome band
            21q22 and mouse chromosome 16
  JOURNAL   Cytogenet Cell Genet 70 (1-2), 48-51 (1995)
   PUBMED   7736788
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000248.1, AP000247.2 and
            AP000246.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001353694.1.
            
            Summary: This gene encodes a RAC1-specific guanine nucleotide
            exchange factor (GEF). GEFs mediate the exchange of guanosine
            diphosphate (GDP) for guanosine triphosphate (GTP). The binding of
            GTP induces a conformational change in RAC1 that allows downstream
            effectors to bind and transduce a signal. This gene thus regulates
            RAC1 signaling pathways that affect cell shape, migration,
            adhesion, growth, survival, and polarity, as well as influencing
            actin cytoskeletal formation, endocytosis, and membrane
            trafficking. This gene thus plays an important role in cell
            invasion, metastasis, and carcinogenesis. In addition to RAC1, the
            encoded protein activates additional Rho-like GTPases such as
            CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein
            isoforms that experience a diverse array of intramolecular,
            protein-protein, and phosphorylation interactions as well as
            phosphoinositide binding. Both the longer and shorter isoforms have
            C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains
            while only the longer isoforms of this gene have the N-terminal
            myristoylation site and the downstream N-terminal PH domain,
            ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain.
            [provided by RefSeq, Jul 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.1711769.1,
                                           SRR1803611.140493.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000541036.6/ ENSP00000441570.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AP000248.1         69244-69367         c
            125-304             AP000248.1         64349-64528         c
            305-481             AP000248.1         1839-2015           c
            482-1455            AP000247.2         102579-103552       c
            1456-1903           AP000247.2         88311-88758         c
            1904-2076           AP000247.2         82057-82229         c
            2077-2301           AP000247.2         62295-62519         c
            2302-2487           AP000247.2         59975-60160         c
            2488-2634           AP000247.2         54122-54268         c
            2635-2709           AP000247.2         49967-50041         c
            2710-2880           AP000247.2         46614-46784         c
            2881-2985           AP000247.2         39477-39581         c
            2986-3067           AP000247.2         31793-31874         c
            3068-3154           AP000247.2         23570-23656         c
            3155-3379           AP000247.2         18991-19215         c
            3380-3483           AP000247.2         1532-1635           c
            3484-3663           AP000246.1         84389-84568         c
            3664-3732           AP000246.1         83208-83276         c
            3733-3858           AP000246.1         82778-82903         c
            3859-3967           AP000246.1         77037-77145         c
            3968-4147           AP000246.1         71467-71646         c
            4148-4266           AP000246.1         71260-71378         c
            4267-4375           AP000246.1         66075-66183         c
            4376-4434           AP000246.1         61032-61090         c
            4435-4537           AP000246.1         60355-60457         c
            4538-4625           AP000246.1         57207-57294         c
            4626-4798           AP000246.1         54664-54836         c
            4799-7218           AP000246.1         48560-50979         c
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.11"
     gene            1..7218
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /note="TIAM Rac1 associated GEF 1"
                     /db_xref="GeneID:7074"
                     /db_xref="HGNC:HGNC:11805"
                     /db_xref="MIM:600687"
     exon            1..124
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            125..304
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            305..481
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            482..1455
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             493..5268
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /note="isoform 1 is encoded by transcript variant 12;
                     human T-lymphoma invasion and metastasis inducing TIAM1
                     protein; T-lymphoma invasion and metastasis-inducing
                     protein 1; T cell lymphoma invasion and metastasis 1; rho
                     guanine nucleotide exchange factor TIAM1"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor TIAM1
                     isoform 1"
                     /protein_id="NP_001340623.1"
                     /db_xref="CCDS:CCDS13609.1"
                     /db_xref="GeneID:7074"
                     /db_xref="HGNC:HGNC:11805"
                     /db_xref="MIM:600687"
                     /translation="MGNAESQHVEHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASS
                     GKVIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVS
                     YTDSSVTPSVDSSIVLTAASVQSMPDTEESRLYGDDATYLAEGGRRQHSYTSNGPTFM
                     ETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARGMETRA
                     SPRQLSTCQRANSLGDLYAQKNSGVTANGGPGSKFAGYCRNLVSDIPNLANHKMPPAA
                     AEETPPYSNYNTLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQDVNAGEGSEFADS
                     GIEGATTDTDLLSRRSNATNSSYSPTTGRAFVGSDSGSSSTGDAARQGVYENFRRELE
                     MSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKK
                     NKKVESATRRKWKHYWVSLKGCTLFFYESDGRSGIDHNSIPKHAVWVENSIVQAVPEH
                     PKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTLRLLKSE
                     IKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCY
                     LASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAM
                     SRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLEGIFDDIVPDGKR
                     EKEVVLPNVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTH
                     QLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICPKVTQSIHIEKSDTAAD
                     TYGFSLSSVEEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAADALNSSMLKDF
                     LSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPLAFLTSNPGHSLCSEQGS
                     SAETAPEETEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQSPSPQDSTGPQLA
                     TMRQLSDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNL
                     TEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFC
                     ASHTKVPKVLVKAKTDTAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLRELF
                     ALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADL
                     SMGDLLLHTTVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRL
                     SIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCS
                     SPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAV
                     SAPSKSLGRRRRRLARNRFTIDSDAVSASSPEKESQQPPGGGDTDRWVEEQFDLAQYE
                     EQDDIKETDILSDDDEFCESVKGASVDRDLQERLQATSISQRERGRKTLDSHASRMAQ
                     LKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI"
     exon            1456..1903
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..2076
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2301
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2487
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2634
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2635..2709
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2880
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..2985
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3067
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3154
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3379
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3380..3483
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3484..3663
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3664..3732
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3858
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3859..3967
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3968..4147
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4148..4266
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4375
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4376..4434
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4435..4537
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4538..4625
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4626..4798
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4799..7218
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7199..7204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /note="hexamer: AATAAA"
     polyA_site      7218
                     /gene="TIAM1"
                     /gene_synonym="NEDLDS; TIAM-1"
                     /note="major polyA site"
ORIGIN      
        1 acaagttaca cccactgcct ccgccagcag ccggggaagg ctgctgcttc ctcatttctg
       61 ttactggaag tttgggacat gtgaagccag acggggagaa acgtgcgcgc cttgttgagc
      121 agcggtagaa tcactgtgga ctgagaccca cctttctaga cctgaagccc aggaggagga
      181 agaggaggct ggttggtacc atgggcataa tgctctgaat cctagtctct cacctagtat
      241 gtgagcagtc cctgcagatg gcccatttgg agatcttgac aaagcctctt ctgtttccaa
      301 tggggttttt ggcgcattct cacagactta gatgaaactg tgatggccac cgcagggggc
      361 aggtgctgac atcgtcccca gccctgtggc tgttcatccg gacatcattt ccaacctcaa
      421 tatctaaatg ccacagtgct cttggagcaa gttgggctgg ggaccactgt tgccttttaa
      481 gaccataaaa ccatgggaaa cgcagaaagt caacatgtag agcacgagtt ttatggagaa
      541 aagcatgcca gcctggggcg caagcacact tcccgctccc tgcgcctctc gcacaagacg
      601 cggaggacca ggcacgcttc ctcggggaag gtgatccaca ggaactccga agtgagcacc
      661 cgatccagca gcacccccag catcccccag tccctggctg aaaatggcct ggagcccttc
      721 tcccaagatg gtaccctaga agacttcggg agccccatct gggtggaccg agtggacatg
      781 ggcttgagac ctgtgtctta cactgactct tctgtcactc ccagcgtaga cagcagcatc
      841 gtcctcacag cagcctctgt gcagagcatg ccagacactg aggagagcag gctttacggg
      901 gatgacgcta catatttggc tgagggaggc aggaggcagc attcctatac atccaatggg
      961 cccactttca tggagacggc gagctttaag aagaaacgct ccaaatctgc agacatctgg
     1021 cgggaggaca gcctggaatt ctcactctct gatctgagcc aagaacattt aacaagcaac
     1081 gaagaaatct tgggttccgc cgaagagaag gactgcgagg aggctcgggg gatggaaacg
     1141 cgggcgagtc cgcggcagct cagcacctgt cagagagcca attccttggg tgacttgtat
     1201 gctcagaaaa actctggagt gacagcaaac ggggggccgg ggagcaaatt tgcaggctac
     1261 tgtcggaatt tggtgtctga tattcccaat cttgcaaacc ataagatgcc accagctgct
     1321 gctgaagaga ctcctccgta cagtaattat aacacacttc cctgtaggaa atctcactgt
     1381 ctctctgaag gtgccaccaa cccacaaatt agccatagca acagcatgca aggcagaaga
     1441 gctaaaacaa ctcaggatgt taatgcaggc gagggcagtg agtttgcaga cagtgggatt
     1501 gaaggggcca ctaccgacac ggacctcctg tccaggcgat ctaatgccac caactccagc
     1561 tactcaccca ccacaggccg ggcctttgtg ggcagcgaca gcggcagcag ctccaccggg
     1621 gatgcggctc gtcagggggt gtacgagaac ttccggcggg agctggagat gagcaccacc
     1681 aacagcgaga gcctggagga ggccggctcg gcgcacagcg atgagcagag cagcggcacc
     1741 ctgagctctc cgggccagtc ggacatcctg ctgaccgccg cacagggcac ggtgcgcaag
     1801 gccggcgccc tggccgtcaa gaacttcctg gtgcacaaga agaacaagaa ggtggagtca
     1861 gccacccgga ggaagtggaa gcactactgg gtgtccctga aaggatgcac gctatttttc
     1921 tacgagagcg acggcaggtc tgggatagac cacaacagca tccccaaaca cgccgtctgg
     1981 gtggagaaca gcattgtgca ggcggtgcct gagcacccca agaaggactt tgtcttctgc
     2041 ctcagcaatt ccctgggtga tgccttcctt tttcagacca ctagccagac ggagcttgaa
     2101 aactggatca ccgccatcca ctctgcctgc gccactgcgg tcgcgaggca ccaccacaag
     2161 gaagacacgc tccgactcct gaaatcagag atcaaaaaac tggaacagaa gattgacatg
     2221 gatgaaaaga tgaagaaaat gggtgaaatg cagctgtctt cagtcactga ctcaaagaaa
     2281 aagaaaacaa tattagatca gatctttgtc tgggagcaaa atctcgagca gttccaaatg
     2341 gacctgtttc gtttccgctg ttatttagcc agccttcagg gtggggagct gccaaacccc
     2401 aaaaggcttc tcgcttttgc aagtcgacca acgaaagtgg ccatgggccg ccttggaatc
     2461 ttttcggtat catcgtttca tgccctggtg gcagcacgca ctggtgaaac tggagtgaga
     2521 agacgtactc aggccatgtc cagatccgcg agcaagcgaa ggagcaggtt ttcttctctg
     2581 tggggtctgg atactacctc caaaaagaag cagggacggc caagcatcaa tcaggtgttt
     2641 ggagagggaa ccgaagctgt aaagaaatct ttagagggaa tatttgatga cattgttcca
     2701 gatggcaaga gggagaaaga agtggtctta cctaacgttc accagcacaa ccctgactgc
     2761 gacatttggg tccacgagta tttcactcca tcctggttct gtctgcccaa taatcagcct
     2821 gccctgacgg tcgtccggcc aggcgacact gcacgggaca ccctggagct gatttgcaag
     2881 acacatcaac tggatcattc tgctcattac ctgcgcctga aatttctaat agaaaacaaa
     2941 atgcagctct atgttccaca gcccgaggaa gacatctatg agctgctgta caaagaaatt
     3001 gaaatctgtc caaaagtcac tcagagcatc cacattgaga agtcagatac agctgctgat
     3061 acttacgggt tttcactttc ttctgtggaa gaagatggta ttcgaaggct gtacgtgaat
     3121 agtgtgaagg aaaccggttt agcttccaag aaaggcctga aagcaggaga tgagattctt
     3181 gagatcaata atcgtgctgc tgacgccctg aactcttcta tgctcaaaga tttcctctca
     3241 cagccctcgc tgggcctcct ggtgaggacc taccccgagc tggaggaagg agtggagctg
     3301 ctggaaagcc cgccccaccg agtggacggc cctgccgacc ttggcgagag ccccctcgcc
     3361 tttctcacca gcaacccagg gcacagcctt tgcagcgagc agggcagcag tgctgagacc
     3421 gctccagagg agaccgaggg gccagacttg gaatcctcag atgagactga tcacagcagc
     3481 aagagtacag aacaggtggc cgcattttgc cgcagtttgc atgagatgaa cccctctgac
     3541 cagagcccat ctcctcagga ctccacgggg cctcagctgg cgaccatgag acaactctcg
     3601 gatgcagata agctgcgcaa ggtgatctgc gagctcctgg agacggagcg cacctacgtg
     3661 aaggatttaa actgtcttat ggagagatac ctaaagcctc ttcaaaaaga aacttttctc
     3721 acccaggatg agcttgacgt gctttttgga aatttaacgg aaatggtaga gtttcaagta
     3781 gaattcctta aaactctaga agatggagtg agactggtac ctgatttgga aaagcttgag
     3841 aaggttgatc aatttaagaa agtgctgttc tctctggggg gatcattcct gtattatgct
     3901 gaccgcttca agctctacag tgccttctgc gccagccaca caaaagttcc caaggtcctg
     3961 gtgaaagcca agacagacac ggctttcaag gcattcttgg atgcccagaa cccgaagcag
     4021 cagcactcat ccacgctgga gtcgtacctc atcaagccca tccagaggat cctcaagtac
     4081 ccacttctgc tcagggagct gttcgccctg accgatgcgg agagcgagga gcactaccac
     4141 ctggacgtgg ccatcaagac catgaacaag gttgccagtc acatcaatga gatgcagaaa
     4201 atccatgaag agtttggggc tgtgtttgac cagctgattg ctgaacagac tggtgagaaa
     4261 aaagaggttg cagatctgag catgggagac ctgcttttgc acactaccgt gatctggctg
     4321 aacccgccgg cctcgctggg caagtggaaa aaggaaccag agttggcagc attcgtcttc
     4381 aaaactgctg tggtccttgt gtataaagat ggttccaaac agaagaagaa acttgtagga
     4441 tctcacaggc tttccattta tgaggactgg gaccccttca gatttcgaca catgatcccc
     4501 acggaagcgc tgcaggttcg agctttggcg agtgcagatg cagaggcaaa tgccgtgtgt
     4561 gaaattgtcc atgtaaaatc cgagtctgaa gggaggccgg agagggtctt tcacttgtgc
     4621 tgcagctccc cagagagccg aaaggatttc ctaaaggctg tgcattcaat cctgcgtgat
     4681 aagcacagaa gacagctcct caaaaccgag agccttccct catcccagca atatgtccct
     4741 tttggaggca aaagattgtg tgcactgaag ggggccaggc cggccatgag cagggcagtg
     4801 tctgccccaa gcaagtctct tgggaggagg aggcggcggc tggctcgaaa caggtttacc
     4861 attgattctg atgccgtctc cgcaagcagc ccggagaaag agtcccagca gccccccggt
     4921 ggtggggaca ctgaccgatg ggtagaggag cagtttgatc ttgctcagta tgaggagcaa
     4981 gatgacatca aggagacaga catcctcagt gacgatgatg agttctgtga gtccgtgaag
     5041 ggtgcctcag tggacagaga cctgcaggag cggcttcagg ccacctccat cagtcagcgg
     5101 gaaagaggcc ggaaaaccct ggatagtcac gcgtcccgca tggcacagct caagaagcaa
     5161 gctgccctgt cggggatcaa tggaggcctg gagagcgcaa gcgaggaagt catttgggtt
     5221 aggcgtgaag actttgcccc ctccaggaaa ctgaacactg agatctgact gcgtcacctg
     5281 ccccgtagag aatgtgtgta gatacttcct gccctaactc tgcccaccct cctgtaccgt
     5341 cgacaagaat gtccccttag gtcgcgctct tgcacacacg gttttggcag ctgacttggt
     5401 tctgaagcca tgtagccacc caactttgtc attttcaaca acatcagaaa gaattgatca
     5461 gaatcccaaa taagcttgag tcctatcttc tgtatattac taagggcttt tatttattct
     5521 caataaatca gggcctgaac aattaaaaga aaaaagattc tatagcactg gaaagcaaat
     5581 caccccagga gttaacggat gtacaacaga ttaatttaag ggatagtagc acacacacga
     5641 tccttctatc tgaaatcagt ctcctagctg gggaaacctc tttcacacac aaaatgaaat
     5701 gtgtacagct tgccgtgttc tgactgtacc cttccctctt ccatgtctga gaatctccgt
     5761 gtattttaag aatgtgtgag gagagggtgg cgattcatgt ttcaatgagc ctcttttttt
     5821 ttttccttcc tgttttggtc tatggctggt cttactctgt gtccatgttc ggaagctcta
     5881 gttttgcata gaattataga gatgccaaac tctttgaaaa gagatccaaa tttatcgctt
     5941 gagagaaaga aaagaaacac tattttttgt attttacctg agatacaggg gcacaaatag
     6001 atgagaattt tacagtgtta gtgtatgtat ccctgagcct aaaaaatgag gatataacct
     6061 tttacagaga gagtgaggcg tggtggtttt atatttatat atgaaaggcc agcaagctca
     6121 tgcgaaggat atacttttct tccaaaaagc ggattttttt tttttaatgt ttgaatctat
     6181 atttgagatg ggagtttggt tggattaaac atgacacccc ggtgggcggt gtgtgtgtct
     6241 gttgcacatg gcagggaggg gagcctcctt ctcatggggt tgccatggtg atcattggtt
     6301 tttccatcaa aattgcatct tcatccatag attaccttcc ccttccctga cagtccataa
     6361 ccaaaccttt aaacagaaca acctctttaa aaacttctct tgtgtttaac actttcttca
     6421 tgccaacgaa acagggtaaa catgctcaaa acattaacag tctaaacaga tatccaaata
     6481 ctaagaagaa aaacaagtta tagcactttc aatttttttt ttttttttaa aaaaaggttt
     6541 atagcttttt cttttcccat gtcacaatgt ccacttccta agaagggttt aaaatactat
     6601 gaaaactttc tttttgggga aaatatctat ttggtgtttg acacatcagt aggtacttta
     6661 aagacctgaa ttttatagta gctttaggag ttatatttta taaaaatcag ttatgacttt
     6721 atatttccag acaatagaga gttcagtaca tcatgctctt gtgcctctgc ctgcttttcc
     6781 tgcgttccca ccctgtattc cccccgcctt tcgggtttcc agggcttcga gcttgatctt
     6841 ttgaaagttt tattctatta aatttttgct atatcttctg gttttctgaa aaagctttag
     6901 aatggtttct ataccctttg tatcactgca tttttccata tcatctccgg ttcgatcgcg
     6961 tccagatgga aaacggaagc agaggcttct aatcgtcgca tttactggct ccagtgcaac
     7021 acatccatct gaaaacactc ggaagtctgg tgcttggaga gggtgccatt gtctcttgta
     7081 cataaggtca tgacgtgtct atgtcaaaag ttcttatata tttcttttat aagctgaaag
     7141 aaggtctatt tttatgtttt taggtctatg aatggaacgt tgtaaatgct tgtcaaacaa
     7201 taaaaataac gaaaagtg
//
