LOCUS       NM_001114092            3754 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens THUMP domain 3 tRNA guanosine methyltransferase
            (THUMPD3), transcript variant 2, mRNA.
ACCESSION   NM_001114092
VERSION     NM_001114092.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Klimontova,M., Chung,K.C.K., Zhang,H., Kouzarides,T.,
            Bannister,A.J. and Hili,R.
  TITLE     PhOxi-seq Detects Enzyme-Dependent m2G in Multiple RNA Types
  JOURNAL   ACS Chem Biol 19 (12), 2399-2405 (2024)
   PUBMED   39611406
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Wang,C., Ulryck,N., Herzel,L., Pythoud,N., Kleiber,N.,
            Guerineau,V., Jactel,V., Moritz,C., Bohnsack,M.T., Carapito,C.,
            Touboul,D., Bohnsack,K.E. and Graille,M.
  TITLE     N 2-methylguanosine modifications on human tRNAs and snRNA U6 are
            important for cell proliferation, protein translation and pre-mRNA
            splicing
  JOURNAL   Nucleic Acids Res 51 (14), 7496-7519 (2023)
   PUBMED   37283053
  REMARK    Erratum:[Nucleic Acids Res. 2024 Jul 22;52(13):8033. doi:
            10.1093/nar/gkae524.. PMID: 38888115]
REFERENCE   3  (bases 1 to 3754)
  AUTHORS   Brumele,B., Mutso,M., Telanne,L., Ounap,K., Spunde,K., Abroi,A. and
            Kurg,R.
  TITLE     Human TRMT112-Methyltransferase Network Consists of Seven Partners
            Interacting with a Common Co-Factor
  JOURNAL   Int J Mol Sci 22 (24), 13593 (2021)
   PUBMED   34948388
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3754)
  AUTHORS   Yang,W.Q., Xiong,Q.P., Ge,J.Y., Li,H., Zhu,W.Y., Nie,Y., Lin,X.,
            Lv,D., Li,J., Lin,H. and Liu,R.J.
  TITLE     THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range
            of tRNA substrates
  JOURNAL   Nucleic Acids Res 49 (20), 11900-11919 (2021)
   PUBMED   34669960
  REMARK    GeneRIF: THUMPD3-TRMT112 is a m2G methyltransferase working on a
            broad range of tRNA substrates.
REFERENCE   5  (bases 1 to 3754)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3754)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   7  (bases 1 to 3754)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   8  (bases 1 to 3754)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA713744.1, AK001680.1, AL117483.1 and AC018506.5.
            
            On Nov 22, 2018 this sequence version replaced NM_001114092.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001622.1, SRR1803611.218903.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000452837.7/ ENSP00000395893.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DA713744.1         22-36
            16-1410             AK001680.1         1-1395
            1411-1833           AL117483.1         855-1277
            1834-3754           AC018506.5         124067-125987       c
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..3754
                     /gene="THUMPD3"
                     /note="THUMP domain 3 tRNA guanosine methyltransferase"
                     /db_xref="GeneID:25917"
                     /db_xref="HGNC:HGNC:24493"
     exon            1..74
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            75..379
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1651
                     /gene="THUMPD3"
                     /EC_number="2.1.1.256"
                     /note="THUMP domain-containing protein 3; tRNA
                     (guanine(6)-N2)-methyltransferase THUMP3; THUMP domain
                     containing 3"
                     /codon_start=1
                     /product="tRNA (guanine(6)-N(2))-methyltransferase THUMP3"
                     /protein_id="NP_001107564.1"
                     /db_xref="CCDS:CCDS2573.1"
                     /db_xref="GeneID:25917"
                     /db_xref="HGNC:HGNC:24493"
                     /translation="MCDIEEATNQLLDVNLHENQKSVQVTESDLGSESELLVTIGATV
                     PTGFEQTAADEVREKLGSSCKISRDRGKIYFVISVESLAQVHCLRSVDNLFVVVQEFQ
                     DYQFKQTKEEVLKDFEDLAGKLPWSNPLKVWKINASFKKKKAKRKKINQNSSKEKINN
                     GQEVKIDQRNVKKEFTSHALDSHILDYYENPAIKEDVSTLIGDDLASCKDETDESSKE
                     ETEPQVLKFRVTCNRAGEKHCFTSNEAARDFGGAVQDYFKWKADMTNFDVEVLLNIHD
                     NEVIVGIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCDPLPYDIIVDPMCGTGAIP
                     IEGATEWSDCFHIAGDNNPLAVNRAANNIASLLTKSQIKEGKPSWGLPIDAVQWDICN
                     LPLRTGSVDIIVTDLPFGKRMGSKKRNWNLYPACLREMSRVCTPTTGRAVLLTQDTKC
                     FTKALSGMRHVWRKVDTVWVNVGGLRAAVYVLIRTPQAFVHPSEQDGERGTLWQCKE"
     exon            380..457
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            458..934
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1065
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1135
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1251
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1362
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1486
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..3754
                     /gene="THUMPD3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1833
                     /gene="THUMPD3"
                     /note="major polyA site"
     regulatory      2639..2644
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THUMPD3"
                     /note="hexamer: ATTAAA"
     polyA_site      2666
                     /gene="THUMPD3"
                     /note="major polyA site"
     regulatory      3498..3503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THUMPD3"
                     /note="hexamer: AATAAA"
     polyA_site      3525
                     /gene="THUMPD3"
     regulatory      3729..3734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THUMPD3"
                     /note="hexamer: AATAAA"
     polyA_site      3754
                     /gene="THUMPD3"
ORIGIN      
        1 ggcttccggc ggcgtgacct gaccgcaaga ggccaatgga gtgtgggagc tgaaagggtc
       61 ttcgctggcg gccggacagt actgctttta aagagacagt gttagggatc ttggaagcac
      121 agccaacatg tgtgacattg aagaagccac taaccaactc ctagatgtga accttcatga
      181 gaaccagaag tctgtacaag tgacagaaag tgacctcgga agtgaatctg agcttctagt
      241 cactattgga gccactgtac ctactggctt tgagcaaaca gctgcagatg aagtcagaga
      301 gaaacttggg tcatcatgca aaatcagcag agaccgtggc aagatatatt ttgtcatttc
      361 agtggaaagt ctggcacagg ttcattgtct gagatcagtt gataacttat ttgtggtggt
      421 tcaggagttt caagattacc agttcaaaca aacaaaggaa gaagttctaa aggattttga
      481 agacttggct ggaaaactcc catggtcaaa ccccttaaaa gtgtggaaaa ttaatgccag
      541 ttttaaaaag aaaaaagcaa agcgcaaaaa gataaatcag aattcaagta aagagaagat
      601 taataatgga caagaagtca aaatcgatca gagaaatgtt aaaaaagagt tcactagcca
      661 tgctttagat tctcatatct tagattatta tgaaaatcca gccatcaaag aggatgtatc
      721 aacattaata ggtgatgatt tggcatcttg caaagatgag actgatgaaa gctcaaaaga
      781 agaaactgag cctcaagtgc tgaagtttag agtcacatgc aacagggcag gagagaaaca
      841 ttgctttacc tcaaatgagg ctgcaagaga ttttgggggt gctgttcaag attattttaa
      901 gtggaaggcc gacatgacca actttgatgt ggaggttctt ttgaacatcc atgataatga
      961 agtcattgtg ggcattgcat tgactgaaga gagtctccac cgaagaaata taacacattt
     1021 tggacctaca actcttagat caactcttgc ctatgggatg ctcaggctct gtgatcctct
     1081 accttatgat ataatagtcg atccaatgtg tggaactggg gcaataccaa tagagggggc
     1141 cactgaatgg tctgactgtt tccatattgc tggtgataat aatccactgg ctgtgaatag
     1201 agcagcaaat aacattgcat ctttattgac caagagccaa attaaagaag gcaaaccctc
     1261 ctggggcttg cccatagatg ctgttcagtg ggatatctgc aatctgccat tgagaactgg
     1321 ctctgtggat attattgtaa cagatttgcc atttggaaaa aggatgggat ccaagaagag
     1381 aaactggaac ctttatccag cttgcctacg ggagatgagc cgtgtctgca cacctaccac
     1441 aggccgagct gtactactta ctcaagacac aaaatgcttt accaaggcgt tatctggaat
     1501 gcgacacgta tggcgaaagg tggatacagt ctgggtgaac gttggtggtc ttcgtgctgc
     1561 agtttacgtt ctgatacgta cacctcaagc ttttgttcat ccttcagaac aagacggaga
     1621 aagaggaact ctttggcaat gcaaagaatg aagatgacta atagtacttg tacttcccac
     1681 cactggaaat gttagcataa aagaacttgg agaggaaaaa agtattaaca aaactgcagt
     1741 ctgcactctt taaacctgtt taaggctctt catcctggtt agcaaaaggt gtgaatgtaa
     1801 tgtgatggaa tttaaaagtt ttatgagacc aggcacagtg gctcacgact gtaattccag
     1861 cagtttagga agccgaagtg tgcagatcac ctgaggtccg gagaccagcc tggccaacat
     1921 ggtgaaaccc tgtctctact agaaatacaa aaattagcca ggtgtggtgg cgggcgcctg
     1981 taatcccaac tactcaggag gctgaggcta gagaatcact tgaacccagc aggcggaggt
     2041 tgcggtgagt cgagatcacg ccattgcact ccagcctgtg cgacaagagc gaaactctgt
     2101 ctcaaaaaga ttttataaga aagcagagct tttccttgaa gctcttttga agtggtagct
     2161 taattagtat tttgttgaaa atactttaaa gatgcctagt gaaaagccta ctaaagtgct
     2221 gtgagattgg ggtttagaac attttatttt caggctttat ggcctatttt ccattgtgtc
     2281 aagtgcaaaa ctaccctggc ccaaaggaag ggcagagaac ataattacat cttaggccac
     2341 atttcattct ttgcagcttt gctaatccag ttgctcaagt tctttacctc aacctgaagg
     2401 aatgaagcat tattacattt tgtgaatggt gttcagccta taagacgaag tctcacaaga
     2461 cctataggaa agtttaccat ctgccttaaa tattaaaata tccatctgtt attcacatta
     2521 atacacagaa tgaaaataga ggtctaggaa ttatcaaagg tactgcaaaa atactgtata
     2581 gaacttattt gtaaatagca catacattga tagatggggt gtgggaccaa caaaccaaat
     2641 taaaagaaat tgtttttctt tcagtacact aaggtgtgtg ttttcaaaac caaacacaaa
     2701 cctgtcaaga gtatttgttg gctagtttct taaaaggcca agagaaaaaa aatgatatat
     2761 ctttggaaaa cttttaattt gggagttatg tgagttgtta ctttctcctg gatcacgatt
     2821 cacatttaat tgcttttcca gttgcaacaa gtccttcttg agagttttgt ggaaaatggt
     2881 gcttccctgg aacaaagagt agttgaggtt tttttgtgga actcatcata gtattgttta
     2941 ttttggagcc tctggctgca atcagtatag attttcagta tcctattagt attataatag
     3001 tactcctctt aatactttct gatgtctcca ttgagaataa aaggacacaa tacttttgaa
     3061 agaagagttc acattagcag cacagccagc aatcctcatc tggtttctct gatctcctac
     3121 cccacacttc ataatgaatg ggaaataaga tgtttatgaa ggttttatat cctagactgg
     3181 cctttttacc actatcagaa ttcacagctg ctttgtttat gggccgaaca caaaactttt
     3241 cacctgatgg gagaaaccat ttaaacctca aattaagcaa cccacagaac caggaagttc
     3301 aaggaccatg tctgttttca ccacgatgtc tttccccacc cccccacccc ccactccacc
     3361 ccccactaag ggcagggtat tgtatctgcc agactgggta tttgttgaac aagcgagtat
     3421 tttcgcctat tagcttagtt tttaaggaaa tcatttttta cttgattcat catagcttta
     3481 attctattac atactacaat aaaaatttga caagactgat acaaatatgt agtgggcaat
     3541 agtttgccgt cttcttccct agtatggtgt ttttcaatct ggtgactaga ataggcagtg
     3601 ggctataagc aggattcata aggcctggag ctgagttata tgtgacactg ccacctattc
     3661 attgtgtgac cttggtttta accttcaaag tgggtctcct ggactaaaag aatgtgaaaa
     3721 gatggggaaa taaatctgta atctgaacat ggaa
//
