LOCUS       NM_015204              10655 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens thrombospondin type 1 domain containing 7A (THSD7A),
            mRNA.
ACCESSION   NM_015204 XM_928187 XM_935904 XM_937164
VERSION     NM_015204.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Tang,X.L., DU,Y.Y., Yu,J., Ye,T., Zhu,H., Chen,Y.F. and Li,X.H.
  TITLE     [Clinical Significance of Thrombospondin Type 1 Domain-Containing
            7A and Neural Epidermal Growth Factor-Like 1 Protein in M-Type
            Phospholipase A2 Receptor-Negative Membranous Nephropathy]
  JOURNAL   Zhongguo Yi Xue Ke Xue Yuan Xue Bao 45 (2), 235-244 (2023)
   PUBMED   37157070
  REMARK    GeneRIF: [Clinical Significance of Thrombospondin Type 1
            Domain-Containing 7A and Neural Epidermal Growth Factor-Like 1
            Protein in M-Type Phospholipase A2 Receptor-Negative Membranous
            Nephropathy].
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   Zhang,R., Shi,Y., Lu,Y., Wu,Y., Chen,M., Fan,Y., Yuan,L. and Mao,R.
  TITLE     Comprehensively characterizing cellular changes and the expression
            of THSD7A and PLA2R1 under multiple in vitro models of podocyte
            injury
  JOURNAL   Cell Biochem Funct 40 (5), 501-515 (2022)
   PUBMED   35670653
  REMARK    GeneRIF: Comprehensively characterizing cellular changes and the
            expression of THSD7A and PLA2R1 under multiple in vitro models of
            podocyte injury.
REFERENCE   3  (bases 1 to 10655)
  AUTHORS   Chen,M., Zhang,L., Zhong,W., Zheng,K., Ye,W. and Wang,M.
  TITLE     Case Report: THSD7A-Positive Membranous Nephropathy Caused by
            Tislelizumab in a Lung Cancer Patient
  JOURNAL   Front Immunol 12, 619147 (2021)
   PUBMED   34040602
  REMARK    GeneRIF: Case Report: THSD7A-Positive Membranous Nephropathy Caused
            by Tislelizumab in a Lung Cancer Patient.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10655)
  AUTHORS   Kaya,B., Paydas,S., Balal,M., Eren Erdogan,K. and Gonlusen,G.
  TITLE     Renal expression of PLA2R, THSD7A, and IgG4 in patients with
            membranous nephropathy and correlation with clinical findings
  JOURNAL   Int J Clin Pract 75 (4), e13855 (2021)
   PUBMED   33249733
  REMARK    GeneRIF: Renal expression of PLA2R, THSD7A, and IgG4 in patients
            with membranous nephropathy and correlation with clinical findings.
REFERENCE   5  (bases 1 to 10655)
  AUTHORS   Suzuki,T., Han,W., Watanabe,S., Terashita,M., Nakata,M.,
            Ichikawa,D., Shirai,S. and Shibagaki,Y.
  TITLE     Clinical characteristics of thrombospondin type-1 domain-containing
            7A-associated membranous nephropathy
  JOURNAL   Ren Fail 42 (1), 966-968 (2020)
   PUBMED   32935600
  REMARK    GeneRIF: Clinical characteristics of thrombospondin type-1
            domain-containing 7A-associated membranous nephropathy.
REFERENCE   6  (bases 1 to 10655)
  AUTHORS   Kuo,M.W., Wang,C.H., Wu,H.C., Chang,S.J. and Chuang,Y.J.
  TITLE     Soluble THSD7A is an N-glycoprotein that promotes endothelial cell
            migration and tube formation in angiogenesis
  JOURNAL   PLoS One 6 (12), e29000 (2011)
   PUBMED   22194972
  REMARK    GeneRIF: THSD7A is a membrane-associated N-glycoprotein with a
            soluble form.
REFERENCE   7  (bases 1 to 10655)
  AUTHORS   Chen,X., Li,X., Wang,P., Liu,Y., Zhang,Z., Zhao,G., Xu,H., Zhu,J.,
            Qin,X., Chen,S., Hu,L. and Kong,X.
  TITLE     Novel association strategy with copy number variation for
            identifying new risk Loci of human diseases
  JOURNAL   PLoS One 5 (8), e12185 (2010)
   PUBMED   20808825
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 10655)
  AUTHORS   Secolin,R., Banzato,C.E., Oliveira,M.C., Bittar,M.F., Santos,M.L.,
            Dalgalarrondo,P. and Lopes-Cendes,I.
  TITLE     Family-based association study for bipolar affective disorder
  JOURNAL   Psychiatr Genet 20 (3), 126-129 (2010)
   PUBMED   20414141
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 10655)
  AUTHORS   Wang,C.H., Su,P.T., Du,X.Y., Kuo,M.W., Lin,C.Y., Yang,C.C.,
            Chan,H.S., Chang,S.J., Kuo,C., Seo,K., Leung,L.L. and Chuang,Y.J.
  TITLE     Thrombospondin type I domain containing 7A (THSD7A) mediates
            endothelial cell migration and tube formation
  JOURNAL   J Cell Physiol 222 (3), 685-694 (2010)
   PUBMED   20020485
  REMARK    GeneRIF: Thrombospondin type I domain containing 7A (THSD7A)
            mediates endothelial cell migration and tube formation.
REFERENCE   10 (bases 1 to 10655)
  AUTHORS   Mori,S., Kou,I., Sato,H., Emi,M., Ito,H., Hosoi,T. and Ikegawa,S.
  TITLE     Association of genetic variations of genes encoding thrombospondin,
            type 1, domain-containing 4 and 7A with low bone mineral density in
            Japanese women with osteoporosis
  JOURNAL   J Hum Genet 53 (8), 694-697 (2008)
   PUBMED   18488137
  REMARK    GeneRIF: Variations may be important determinants of osteoporosis
            in Japanese women.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK092252.1, AC004614.2,
            AB023177.2, AA297188.1, BF111945.1, AK001884.1, AC004160.1 and
            BC030083.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015204.2.
            
            Summary: The protein encoded by this gene is found almost
            exclusively in endothelial cells from placenta and umbilical cord.
            The encoded protein appears to interact with alpha(V)beta(3)
            integrin and paxillin to inhibit endothelial cell migration and
            tube formation. This protein may be involved in cytoskeletal
            organization. Variations in this gene may be associated with low
            bone mineral density in osteoporosis. [provided by RefSeq, Aug
            2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000423059.9/ ENSP00000406482.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-643               AK092252.1         1-643
            644-1105            AC004614.2         100129-100590       c
            1106-3052           AK092252.1         1106-3052
            3053-6385           AB023177.2         2337-5669
            6386-6454           AA297188.1         47-115
            6455-6678           BF111945.1         1-224               c
            6679-7816           AK001884.1         196-1333
            7817-10567          AC004160.1         73555-76305         c
            10568-10585         BC030083.1         877-894
            10586-10655         AC004160.1         73467-73536         c
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p21.3"
     gene            1..10655
                     /gene="THSD7A"
                     /note="thrombospondin type 1 domain containing 7A"
                     /db_xref="GeneID:221981"
                     /db_xref="HGNC:HGNC:22207"
                     /db_xref="MIM:612249"
     exon            1..442
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..5226
                     /gene="THSD7A"
                     /note="thrombospondin type-1 domain-containing protein 7A;
                     thrombospondin, type I, domain containing 7A"
                     /codon_start=1
                     /product="thrombospondin type-1 domain-containing protein
                     7A precursor"
                     /protein_id="NP_056019.1"
                     /db_xref="CCDS:CCDS47543.1"
                     /db_xref="GeneID:221981"
                     /db_xref="HGNC:HGNC:22207"
                     /db_xref="MIM:612249"
                     /translation="MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGA
                     GRAAAQGEAEAPTLYLWKTGPWGRCMGDECGPGGIQTRAVWCAHVEGWTTLHTNCKQA
                     ERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSLEKPLECIKGEEGIQVREIAC
                     IQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTCGSGLQHRT
                     RHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARR
                     RGKNKEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKT
                     GKAADLSFCQQEKLPMTFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTI
                     RQFPIGSEKECPEFEEKEPCLSQGDGVVPCATYGWRTTEWTECRVDPLLSQQDKRRGN
                     QTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMDLKLCTGPIPNTTQLCHIP
                     CPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCPHLLEAI
                     PCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIP
                     VACDAPCPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSA
                     LQEVRSCNEHPCTVYHWQTGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICV
                     RVNVGQVGPKKCPESLRPETVRPCLLPCKKDCIVTPYSDWTSCPSSCKEGDSSIRKQS
                     RHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKWRRCQLVPWSVQQDSPG
                     AQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLTSWSK
                     FSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDC
                     ILPEGKVEVLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACII
                     PCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVV
                     PCHSDCNQYLWVTEPWSICKVTFVNMRENCGEGVQTRKVRCMQNTADGPSEHVEDYLC
                     DPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRSADPIRQPADEGRSC
                     PNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVDLK
                     YCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQG
                     DGRPCPSLMDQSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADD
                     FSKVVDEEFCADIELIIDGNKNMVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDL
                     GFGGIQVRSRPVIIQELENQHLCPEQMLETKSCYDGQCYEYKWMASAWKGSSRTVWCQ
                     RSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGYTEVMSSNSTLEQ
                     CTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYG
                     VAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM"
     exon            443..1274
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1523
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1705
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1861
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..2074
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2269
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2504
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2620
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2753
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2857
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..3052
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..3316
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3495
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3501
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3502..3635
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3789
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3790..3934
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            3935..4050
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4051..4168
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4169..4314
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4315..4489
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4490..4663
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4664..4759
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4760..4842
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            4843..5053
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            5054..5141
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     exon            5142..10655
                     /gene="THSD7A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10557..10562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THSD7A"
                     /note="hexamer: CATAAA"
     polyA_site      10585
                     /gene="THSD7A"
                     /note="major polyA site"
     regulatory      10634..10639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THSD7A"
                     /note="hexamer: ATTAAA"
     polyA_site      10655
                     /gene="THSD7A"
ORIGIN      
        1 attctgcgag gaatggcagc ggcagcagca tccccgccag aggcggcggc ggcggcggca
       61 gcggccacgg ccaccaccgc ggacgctatt gttccctggt gttagacgcg ctctccctcc
      121 ttctcatctg aagcgaacaa tagcaggaaa gagctttgct ttctgtactc tttgaaaaga
      181 cgttccaaga gcggagaaaa attccctgcc gagcgtgcta cggctctgga ccctggagtg
      241 gctgcaggcg gcatggggct gcaagccagg cgctgggcgt ccgggagccg gggcgctgcg
      301 gggccgcgcc ggggcgtcct gcagctgctg ccgctgccgc tgccgctgcc gctgctcctg
      361 ctgctgctgc tacgcccggg cgccggcagg gctgcggcgc agggcgaggc ggaggcgccc
      421 accctctatc tgtggaagac tggtccatgg ggccgatgta tgggagatga atgtggtccc
      481 ggaggcatcc aaacgagggc tgtgtggtgt gctcatgtgg agggatggac tacactgcat
      541 actaactgta agcaggccga gagacccaat aaccagcaga attgtttcaa agtttgcgat
      601 tggcacaaag agttgtacga ctggagactg ggaccttgga atcagtgtca gcccgtgatt
      661 tcaaaaagcc tagagaaacc tcttgagtgc attaaggggg aagaaggtat tcaggtgagg
      721 gagatagcgt gcatccagaa agacaaagac attcctgcgg aggatatcat ctgtgagtac
      781 tttgagccca agcctctcct ggagcaggct tgcctcattc cttgccagca agattgcatc
      841 gtgtctgaat tttctgcctg gtccgaatgc tccaagacct gcggcagcgg gctccagcac
      901 cggacgcgtc atgtggtggc gcccccgcag ttcggaggct ctggctgtcc aaacctgacg
      961 gagttccagg tgtgccaatc cagtccatgc gaggccgagg agctcaggta cagcctgcat
     1021 gtggggccct ggagcacctg ctcaatgccc cactcccgac aagtaagaca agcaaggaga
     1081 cgcgggaaga ataaagaacg ggaaaaggac cgcagcaaag gagtaaagga tccagaagcc
     1141 cgcgagctta ttaagaaaaa gagaaacaga aacaggcaga acagacaaga gaacaaatat
     1201 tgggacatcc agattggata tcagaccaga gaggttatgt gcattaacaa gacggggaaa
     1261 gctgctgatt taagcttttg ccagcaagag aagcttccaa tgaccttcca gtcctgtgtg
     1321 atcaccaaag agtgccaggt ttccgagtgg tcagagtgga gcccctgctc aaaaacatgc
     1381 catgacatgg tgtcccctgc aggcactcgt gtaaggacac gaaccatcag gcagtttccc
     1441 attggcagtg aaaaggagtg tccagaattt gaagaaaaag aaccctgttt gtctcaagga
     1501 gatggagttg tcccctgtgc cacgtatggc tggagaacta cagagtggac tgagtgccgt
     1561 gtggaccctt tgctcagtca gcaggacaag aggcgcggca accagacggc cctctgtgga
     1621 gggggcatcc agacccgaga ggtgtactgc gtgcaggcca acgaaaacct cctctcacaa
     1681 ttaagtaccc acaagaacaa agaagcctca aagccaatgg acttaaaatt atgcactgga
     1741 cctatcccta atactacaca gctgtgccac attccttgtc caactgaatg tgaagtttca
     1801 ccttggtcag cttggggacc ttgtacttat gaaaactgta atgatcagca agggaaaaaa
     1861 ggcttcaaac tgaggaagcg gcgcattacc aatgagccca ctggaggctc tggggtaacc
     1921 ggaaactgcc ctcacttact ggaagccatt ccctgtgaag agcctgcctg ttatgactgg
     1981 aaagcagtga gactgggaaa ctgcgagcca gataacggaa aggagtgtgg tccaggcacg
     2041 caagttcaag aggttgtgtg catcaacagt gatggagaag aagttgacag acagctgtgc
     2101 agagatgcca tcttccccat ccctgtggcc tgtgatgccc catgcccgaa agactgtgtg
     2161 ctcagcacat ggtctacgtg gtcctcctgc tcacacacct gctcagggaa aacgacagaa
     2221 gggaaacaga tacgagcacg atccattctg gcctatgcgg gtgaagaagg tggaattcgc
     2281 tgtccaaata gcagtgcttt gcaagaagta cgaagctgta atgagcatcc ttgcacagtg
     2341 taccactggc aaactggtcc ctggggccag tgcattgagg acacctcagt atcgtccttc
     2401 aacacaacta cgacttggaa tggggaggcc tcctgctctg tcggcatgca gacaagaaaa
     2461 gtcatctgtg tgcgagtcaa tgtgggccaa gtgggaccca aaaaatgtcc tgaaagcctt
     2521 cgacctgaaa ctgtaaggcc ttgtctgctt ccttgtaaga aggactgtat tgtgacccca
     2581 tatagtgact ggacatcatg cccctcttcg tgtaaagaag gggactccag tatcaggaag
     2641 cagtctaggc atcgggtcat cattcagctg ccagccaacg ggggccgaga ctgcacagat
     2701 cccctctatg aagagaaggc ctgtgaggca cctcaagcgt gccaaagcta caggtggaag
     2761 actcacaaat ggcgcagatg ccaattagtc ccttggagcg tgcaacaaga cagccctgga
     2821 gcacaggaag gctgtgggcc tgggcgacag gcaagagcca ttacttgtcg caagcaagat
     2881 ggaggacagg ctggaatcca tgagtgccta cagtatgcag gccctgtgcc agcccttacc
     2941 caggcctgcc agatcccctg ccaggatgac tgtcaattga ccagctggtc caagttttct
     3001 tcatgcaatg gagactgtgg tgcagttagg accagaaagc gcactcttgt tggaaaaagt
     3061 aaaaagaagg aaaaatgtaa aaattcccat ttgtatcccc tgattgagac tcagtattgt
     3121 ccttgtgaca aatataatgc acaacctgtg gggaactggt cagactgtat tttaccagag
     3181 ggaaaagtgg aagtgttgct gggaatgaaa gtacaaggag acatcaagga atgcggacaa
     3241 ggatatcgtt accaagcaat ggcatgctac gatcaaaatg gcaggcttgt ggaaacatct
     3301 agatgtaaca gccatggtta cattgaggag gcctgcatca tcccctgccc ctcagactgc
     3361 aagctcagtg agtggtccaa ctggtcgcgc tgcagcaagt cctgtgggag tggtgtgaag
     3421 gttcgttcta aatggctgcg tgaaaaacca tataatggag gaaggccttg ccccaaactg
     3481 gaccatgtca accaggcaca ggtgtatgag gttgtcccat gccacagtga ctgcaaccag
     3541 tacctatggg tcacagagcc ctggagcatc tgcaaggtga cctttgtgaa tatgcgggag
     3601 aactgtggag agggcgtgca aacccgaaaa gtgagatgca tgcagaatac agcagatggc
     3661 ccttctgaac atgtagagga ttacctctgt gacccagaag agatgcccct gggctctaga
     3721 gtgtgcaaat taccatgccc tgaggactgt gtgatatctg aatggggtcc atggacccaa
     3781 tgtgttttgc cttgcaatca aagcagtttc cggcaaaggt cagctgatcc catcagacaa
     3841 ccagctgatg aaggaagatc ttgccctaat gctgttgaga aagaaccctg taacctgaac
     3901 aaaaactgct accactatga ttataatgta acagactgga gtacatgtca gctgagtgag
     3961 aaggcagttt gtggaaatgg aataaaaaca aggatgttgg attgtgttcg aagtgatggc
     4021 aagtcagttg acctgaaata ttgtgaagcg cttggcttgg agaagaactg gcagatgaac
     4081 acgtcctgca tggtggaatg ccctgtgaac tgtcagcttt ctgattggtc tccttggtca
     4141 gaatgttctc aaacatgtgg cctcacagga aaaatgatcc gaagacgaac agtgacccag
     4201 ccctttcaag gtgatggaag accatgccct tccctgatgg accagtccaa accctgccca
     4261 gtgaagcctt gttatcggtg gcaatatggc cagtggtctc catgccaagt gcaggaggcc
     4321 cagtgtggag aagggaccag aacaaggaac atttcttgtg tagtaagtga tgggtcagct
     4381 gatgatttca gcaaagtggt ggatgaggaa ttctgtgctg acattgaact cattatagat
     4441 ggtaataaaa atatggttct ggaggaatcc tgcagccagc cttgcccagg tgactgttat
     4501 ttgaaggact ggtcttcctg gagcctgtgt cagctgacct gtgtgaatgg tgaggatcta
     4561 ggctttggtg gaatacaggt cagatccaga ccggtgatta tacaagaact agagaatcag
     4621 catctgtgcc cagagcagat gttagaaaca aaatcatgtt atgatggaca gtgctatgaa
     4681 tataaatgga tggccagtgc ttggaagggc tcttcccgaa cagtgtggtg tcaaaggtca
     4741 gatggtataa atgtaacagg gggctgcttg gtgatgagcc agcctgatgc cgacaggtct
     4801 tgtaacccac cgtgtagtca accccactcg tactgtagcg agacaaaaac atgccattgt
     4861 gaagaagggt acactgaagt catgtcttct aacagcaccc ttgagcaatg cacacttatc
     4921 cccgtggtgg tattacccac catggaggac aaaagaggag atgtgaaaac cagtcgggct
     4981 gtacatccaa cccaaccctc cagtaaccca gcaggacggg gaaggacctg gtttctacag
     5041 ccatttgggc cagatgggag actaaagacc tgggtttacg gtgtagcagc tggggcattt
     5101 gtgttactca tctttattgt ctccatgatt tatctagctt gcaaaaagcc aaagaaaccc
     5161 caaagaaggc aaaacaaccg actgaaacct ttaaccttag cctatgatgg agatgccgac
     5221 atgtaacata taacttttcc tggcaacaac cagtttcggc tttctgactt catagatgtc
     5281 cagaggccac aacaaatgta tccaaactgt gtggattaaa atatatttta atttttaaaa
     5341 atggcatcat aaagacaaga gtgaaaatca tactgccact ggagatattt aagacagtac
     5401 cacttatata cagaccatca accgtgagaa ttataggaga tttagctgaa tacatgctgc
     5461 attctgaaag ttttatgtca tcttttctga aatctaccga ctgaaaaacc actttcatct
     5521 ctaaaaaata atggtggaat tggccagtta ggatgcctga tacaagaccg tctgcagtgt
     5581 taatccataa aacttcctag catgaagagt ttctaccaag atctccacaa tactatggtc
     5641 aaattaacat gtgtactcag ttgaatgaca cacattatgt cagattatgt acttgctaat
     5701 aagcaatttt aacaatgcat aacaaataaa ctctaagcta agcagaaaat ccactgaata
     5761 aattcagcat cttggtggtc gatggtagat tttattgacc tgcatttcag agacaaagcc
     5821 tcttttttaa gacttcttgt ctctctccaa agtaagaatg ctggacaagt actagtgtct
     5881 tagaagaacg agtcctcaag ttcagtattt tatagtggta attgtctgga aaactaattt
     5941 acttgtgtta atacaatacg tttctacttt ccctgatttt caaactggtt gcctgcatct
     6001 tttttgctat atggaaggca catttttgca ctatattagt gcagcacgat aggcgcttaa
     6061 ccagtattgc catagaaact gcctcttttc atgtgggatg aagacatctg tgccaagagt
     6121 ggcatgaaga catttgcaag ttcttgtatc ctgaagagag taaagttcag tttggatggc
     6181 agcaagatga aatcagctat tacacctgct gtacacacac ttcctcatca ctgcagccat
     6241 tgtgaaattg acaacatggc ggtaatttaa gtgttgaagt ccctaacccc ttaaccctct
     6301 aaaaggtgga ttcctctagt tggtttgtaa ttgttctttg aaggctgttt atgactagat
     6361 ttttatattt gttatctttg ttaagaaaaa aaaagaaaaa ggaactggat gtctttttaa
     6421 ttttgagcag atggagaaaa taaataatgt atcaatgacc tttgtaacta aaggaaatca
     6481 aaattatatg ttgatttttc tttctctctg atttcccagt ttcagattga atgtctgtct
     6541 tgcaggcagt tatttcaaaa tccatagtct tttgcctttc tcactggcaa aatttgaagc
     6601 aatctctctc ttcaatgtat gatttcaaaa ctaaaatttt aaaagagaag aataaatatc
     6661 ttatccaaga cttcattgca tatctagagc aagagtggca aactttttct gtaaaggact
     6721 atatagaaaa tattttccac aaggtctatg ttgcaacttc tcaactttgc cattctgcta
     6781 atgcaatctt agtaaataaa agaatgaaca taactgtttt cctatataac ttcccttaca
     6841 aaaacagcca gtaggccacc tttgcaggtc atattttgct gacccctggt ctggatgttt
     6901 tttaatgcta gctctccatg gtgtcatctt tcgttttcat tcatttttca aaggtgagaa
     6961 tttacagtca ttacatttcc cactgtatca gaagtagctg tattttctac agatgtagcc
     7021 ctgggaatgg cacagtggaa caatatttaa ctaactcagc agacttcttg caaatgaaaa
     7081 gaaaactcaa aaggtgccat taaaaagcaa taccattttc aggcaacatt attgcaacaa
     7141 cctttgtgag agaggacact agcttttcag gatatttgaa aaataatata actaacatgc
     7201 tattttatct taaacagctg tgttcctgta aaagtgtttt ggattagaag gtatttttac
     7261 cttcatactg gagtatgaac tccccctgct gttcattaga gtatttgcaa agattacaaa
     7321 agacatcagg ttaggatgtc attgtcccca aaatgaaaat atttgtatgt atttcaataa
     7381 gaactaaatg cagtaagcgc taattcctca ttcaatattc actatattag gaaatgtaaa
     7441 tcatttcaaa gagaacaggt agtcaggaag atgaaaattt tactttaaag caaggattta
     7501 aacaaagaaa accataaaaa caaagtccat tttatctgtt ttattaaaga ggaagaaaaa
     7561 ataatattgt tactttaaaa ctgtgattgt caatatatat aatcaggggt cttccagata
     7621 atcctaaagt tgatctcttt gaataaaata aactccattt gtccaaaata gttaagaaag
     7681 actctattgt ggttacttga aaaattcagc taaccctctt atgaaatgtg tgcaacacct
     7741 attaattttg aaatgtggga tagaatacct actaaagcta tatttaaggt attgtaaaat
     7801 gtcattaatt cagaaaagat tagtatcatc ctacctataa gaattaaatt agcaaatcaa
     7861 aattatagaa tgttatgaaa aatacatttt attcataaat taattagatt ttacagcata
     7921 tatgcatgca tatatatata tacacacaca cacacacaca cccatatata tgagagagag
     7981 gaaattaggt tcaacatgac ctaatattag agaacatatt ggaaggtcac tgtttgttag
     8041 gataaataaa tactggataa cctcgttaga aaggtggaaa gaggagaacg tagcaatctg
     8101 gctattttgg acaaagggaa ggttattata ttcatgttta taacatgaaa atggccttat
     8161 ttttgtttct gacatgagac aataatgagt taaatatttt attggaattt catcaaaacc
     8221 ttatttttac tgttccttct aaaaatttgg gaggggaata tagtagtaac ttctctattg
     8281 acacttttac ctcaccgagg gggtaaaaga tgtagaactg ctgttcccta gaatgaaggt
     8341 ctgttgtttg gtttttaaaa aaagaagagc agaaaaagtt tgacacagta ttatatgatt
     8401 ttgctggact atttcactag aaaccacgta gaataggact aactgatctc ttttgaaagg
     8461 ggctgatttg cttattcatc aataactaat ccaatatgtg gacagtaatc ttaatttcaa
     8521 agcctttttt gaggatacta cggtgtagct taagtgaatt taaaaagagt gcataaaagc
     8581 ttgtttttaa ttacttataa caaagcacaa attacttcta acaaggcaaa tatttattga
     8641 catataagac attctgctag gcattgtaaa aaaaaaaaaa aaagtaatgg cttttgtata
     8701 cataggtact taagacagac atccaaaaga aggaaaaaat gcttaatgag tgaagatagc
     8761 actgaaacat gttgactttt actctaggtt tgtttttaga tggggttagt attttcccct
     8821 acgtaatgta catgtcttta ggccacagta ttaggagttt gatttttccc tgccccaccc
     8881 tcaggtaggt ggcagtggtc attgtagctt aatcagaccc ctgttaagtt cctgggcatt
     8941 tcaaggtaaa ttcacgtctt ccttctcaca gaaattgaat agacctctca cacacttatt
     9001 tacctccccc aacaacctgt atccttggcc ctttctgatt ttctctgctt ggtctctttc
     9061 tactcttgac agctattctt acctacttcc cactaaacat gcccaatttt ttaattaaaa
     9121 aaaaaagaat tcctatattt ccttccctat tagaatcaaa gtgtcactca ctcagagtgt
     9181 gaacatcctg atttacacca aatccactgg tcaaagaaat gaataaaggg aaaaggaaaa
     9241 atgtggaagg catgcaggaa aaaaaaaaaa aaaaaagccc ttttctgtaa aaatgtccat
     9301 gcctacactt gctttagacc atcccatatc ctcctgcctc catctcgagt ctttgttcac
     9361 attccacacc acaagacagt gagggaggac tgtacttttc agtaccatac agaagggaga
     9421 gcctgttcta aagctgacgt gggtctcaat agtacatgag aagcacaatt cccaaatgat
     9481 ctgggatctc ctgtttataa atatattacc aaattcaagt gtgggttctt cttctatttc
     9541 cactgtaaga ttgagggtgt tgaaaaaaca gtagtccttg agcaaatctg tgaggaatct
     9601 tgtcagaatg tgatctttcc tgggactagt ttagttgtga gaactacatc tgcttgttct
     9661 gggaataagg gtctccccag gtcctgccct caggtggccc tggctgcagg acaggatttc
     9721 aggtatcaga aactggtgtg tgagccaagc aaatagttat ccatttataa aaaggatggt
     9781 ttgcagtgtg ttataataat tttatatcta gcagggacaa tgaaaaatgg ttatgtctac
     9841 attattaaat gaatgaaact aatgctaaac agttaatttt gcatttttat ttttctgtgt
     9901 ttctaccaag tcagatctta aaaatgggca gtatatgaag agctttagtt tgtgggagtc
     9961 atgttttcag tgatcaaaaa tctctatatt atttagaata taattgtcag tcttgttgtg
    10021 ttttgtagat ttattgtttt taagccatag tttctagatt acctactttt aaccctcaag
    10081 taaacttttc cctatttttc agaaatctgt ttgtgtttat ttttagaaag taaacattta
    10141 gattcctttt ctggtacttt ttttctccat ttctatcaca cagatttgaa gagatcagta
    10201 tctatcattg taaaatataa ctttattaaa atttgcaatc tgtctttcta taaattatta
    10261 ttccctaaat gtgtatttta tgttaattta actacctgaa tatttatttt gtttaaataa
    10321 ggaggcagct gtctaagaat ttttgttaat attactttgt aaattgcgta atgtaattta
    10381 ttttacatta tgtacatttc ctttttaacg cacaaaatgt ctatgtacaa ttaactacag
    10441 atttgcaaat aatgtcacta agctttattc agtcaataca gatggcatgt gaagatgtaa
    10501 tatgcttttt tacattttca tatgggttat ttgtaaataa ctatattgct gacaaacata
    10561 aatacggaaa tcattttcat tctaatcatg tgtatctgtg ttttgatttg caccaattgc
    10621 ttcttaaata tggattaaaa tttttttcca gtgaa
//
