LOCUS       NM_024817               9373 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens thrombospondin type 1 domain containing 4 (THSD4),
            transcript variant 1, mRNA.
ACCESSION   NM_024817 XM_005254678 XM_006720692
VERSION     NM_024817.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Liu,J., Huang,Z., Chen,H.N., Qin,S., Chen,Y., Jiang,J., Zhang,Z.,
            Luo,M., Ye,Q., Xie,N., Zhou,Z.G., Wei,Y., Xie,K. and Huang,C.
  TITLE     ZNF37A promotes tumor metastasis through transcriptional control of
            THSD4/TGF-beta axis in colorectal cancer
  JOURNAL   Oncogene 40 (19), 3394-3407 (2021)
   PUBMED   33875786
  REMARK    GeneRIF: ZNF37A promotes tumor metastasis through transcriptional
            control of THSD4/TGF-beta axis in colorectal cancer.
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Elbitar,S., Renard,M., Arnaud,P., Hanna,N., Jacob,M.P., Guo,D.C.,
            Tsutsui,K., Gross,M.S., Kessler,K., Tosolini,L., Dattilo,V.,
            Dupont,S., Jonquet,J., Langeois,M., Benarroch,L., Aubart,M.,
            Ghaleb,Y., Abou Khalil,Y., Varret,M., El Khoury,P., Ho-Tin-Noe,B.,
            Alembik,Y., Gaertner,S., Isidor,B., Gouya,L., Milleron,O.,
            Sekiguchi,K., Milewicz,D., De Backer,J., Le Goff,C., Michel,J.B.,
            Jondeau,G., Sakai,L.Y., Boileau,C. and Abifadel,M.
  TITLE     Pathogenic variants in THSD4, encoding the ADAMTS-like 6 protein,
            predispose to inherited thoracic aortic aneurysm
  JOURNAL   Genet Med 23 (1), 111-122 (2021)
   PUBMED   32855533
REFERENCE   3  (bases 1 to 9373)
  AUTHORS   Cohen,H., Ben-Hamo,R., Gidoni,M., Yitzhaki,I., Kozol,R.,
            Zilberberg,A. and Efroni,S.
  TITLE     Shift in GATA3 functions, and GATA3 mutations, control progression
            and clinical presentation in breast cancer
  JOURNAL   Breast Cancer Res 16 (6), 464 (2014)
   PUBMED   25410484
  REMARK    GeneRIF: In breast cancer, some GATA3 effects shift from tumor
            suppressing to tumor promoting during tumorigenesis, with
            deregulation of three genes, BCL2, DACH1, THSD4, representing major
            GATA3-controlled processes in cancer progression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9373)
  AUTHORS   Naba,A., Clauser,K.R., Whittaker,C.A., Carr,S.A., Tanabe,K.K. and
            Hynes,R.O.
  TITLE     Extracellular matrix signatures of human primary metastatic colon
            cancers and their metastases to liver
  JOURNAL   BMC Cancer 14, 518 (2014)
   PUBMED   25037231
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9373)
  AUTHORS   Deng,X., Sabino,E.C., Cunha-Neto,E., Ribeiro,A.L., Ianni,B.,
            Mady,C., Busch,M.P. and Seielstad,M.
  CONSRTM   REDSII Chagas Study Group from the NHLBI Retrovirus Epidemiology
            Donor Study-II Component International
  TITLE     Genome wide association study (GWAS) of Chagas cardiomyopathy in
            Trypanosoma cruzi seropositive subjects
  JOURNAL   PLoS One 8 (11), e79629 (2013)
   PUBMED   24324551
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9373)
  AUTHORS   Tsutsui,K., Manabe,R., Yamada,T., Nakano,I., Oguri,Y., Keene,D.R.,
            Sengle,G., Sakai,L.Y. and Sekiguchi,K.
  TITLE     ADAMTSL-6 is a novel extracellular matrix protein that binds to
            fibrillin-1 and promotes fibrillin-1 fibril formation
  JOURNAL   J Biol Chem 285 (7), 4870-4882 (2010)
   PUBMED   19940141
REFERENCE   7  (bases 1 to 9373)
  AUTHORS   Apte,S.S.
  TITLE     A disintegrin-like and metalloprotease (reprolysin-type) with
            thrombospondin type 1 motif (ADAMTS) superfamily: functions and
            mechanisms
  JOURNAL   J Biol Chem 284 (46), 31493-31497 (2009)
   PUBMED   19734141
  REMARK    Review article
REFERENCE   8  (bases 1 to 9373)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 9373)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   10 (bases 1 to 9373)
  AUTHORS   Mori,S., Kou,I., Sato,H., Emi,M., Ito,H., Hosoi,T. and Ikegawa,S.
  TITLE     Association of genetic variations of genes encoding thrombospondin,
            type 1, domain-containing 4 and 7A with low bone mineral density in
            Japanese women with osteoporosis
  JOURNAL   J Hum Genet 53 (8), 694-697 (2008)
   PUBMED   18488137
  REMARK    GeneRIF: Variations may be important determinants of osteoporosis
            in Japanese women.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC068181.9, AC015711.11, AC105132.4, AC104943.8, AC026636.7 and
            AC104938.7.
            
            On or before Apr 22, 2021 this sequence version replaced
            XM_006720692.3, NM_024817.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            CCDS Note: The coding region has been updated to include additional
            5' exons and to extend the N-terminus to one that is more supported
            by available transcript and conservation data. The update adds an
            additional TSP1 (Thrombospondin type 1 repeats) domain and an
            Adam_spacer 1 domain to the protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.1397821.1,
                                           SRR14038191.1173323.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261862.8/ ENSP00000261862.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               AC068181.9         79190-79417         c
            229-336             AC068181.9         53332-53439         c
            337-406             AC068181.9         39956-40025         c
            407-771             AC015711.11        72459-72823         c
            772-1219            AC015711.11        44762-45209         c
            1220-1322           AC015711.11        31143-31245         c
            1323-1459           AC105132.4         35406-35542         c
            1460-1664           AC104943.8         18083-18287         c
            1665-1840           AC026636.7         32043-32218         c
            1841-1937           AC026636.7         29550-29646         c
            1938-2213           AC026636.7         22760-23035         c
            2214-2343           AC026636.7         15532-15661         c
            2344-2548           AC026636.7         13725-13929         c
            2549-2722           AC026636.7         12173-12346         c
            2723-2896           AC026636.7         2692-2865           c
            2897-3076           AC104938.7         40364-40543
            3077-3221           AC104938.7         46408-46552
            3222-9373           AC104938.7         52576-58727
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q23"
     gene            1..9373
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /note="thrombospondin type 1 domain containing 4"
                     /db_xref="GeneID:79875"
                     /db_xref="HGNC:HGNC:25835"
                     /db_xref="MIM:614476"
     exon            1..228
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            229..336
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..3364
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; thrombospondin type-1 domain-containing protein
                     4; ADAMTS-like protein 6; A disintegrin and
                     metalloproteinase with thrombospondin motifs-like protein
                     6; thrombospondin, type I, domain containing 4"
                     /codon_start=1
                     /product="thrombospondin type-1 domain-containing protein
                     4 isoform 1 precursor"
                     /protein_id="NP_079093.2"
                     /db_xref="CCDS:CCDS10238.2"
                     /db_xref="GeneID:79875"
                     /db_xref="HGNC:HGNC:25835"
                     /db_xref="MIM:614476"
                     /translation="MVSHFMGSLSVLCFLLLLGFQFVCPQPSTQHRKVPQRMAAEGAP
                     EDDGGGGAPGVWGAWGPWSACSRSCSGGVMEQTRPCLPRSYRLRGGQRPGAPARAFAD
                     HVVSAVRTSVPLHRSRDETPALAGTDASRQGPTVLRGSRHPQPQGLEVTGDRRSRTRG
                     TIGPGKYGYGKAPYILPLQTDTAHTPQRLRRQKLSSRHSRSQGASSARHGYSSPAHQV
                     PQHGPLYQSDSGPRSGLQAAEAPIYQLPLTHDQGYPAASSLFHSPETSNNHGVGTHGA
                     TQSFSQPARSTAISCIGAYRQYKLCNTNVCPESSRSIREVQCASYNNKPFMGRFYEWE
                     PFAEVKGNRKCELNCQAMGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDYL
                     GSDKVVDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPEGATKINITEMYKSNN
                     YLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEISSTAGESFLAEGPTNEIL
                     DVYMIHQQPNPGVHYEYVIMGTNAISPQVPPHRRPGEPFNGQMVTEGRSQEEGEQKGR
                     NEEKEDLRGEAPEMFTSESAQTFPVRHPDRFSPHRPDNLVPPAPQPPRRSRDHNWKQL
                     GTTECSTTCGKGSQYPIFRCVHRSTHEEAPESYCDSSMKPTPEEEPCNIFPCPAFWDI
                     GEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQ
                     IRTDWTSCSVPCGVGQRTRDVKCVSNIGDVVDDEECNMKLRPNDIENCDMGPCAKSWF
                     LTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPAEATPCDNGPCTGKVEW
                     FAGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPSECSFLEKPPSQQSCHLKPCGA
                     KWFSTEWSMCSKSCQGGFRVREVRCLSDDMTLSNLCDPQLKPEERESCNPQDCVPEVD
                     ENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRQTGFLGSR"
     exon            337..406
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            407..771
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            772..1219
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1322
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1459
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1664
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1840
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1937
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2213
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2343
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2548
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2722
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2896
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..3076
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3221
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..9373
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9247..9252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /note="hexamer: AATAAA"
     polyA_site      9266
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /note="major polyA site"
     regulatory      9354..9359
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
                     /note="hexamer: AATAAA"
     polyA_site      9373
                     /gene="THSD4"
                     /gene_synonym="AAT12; ADAMTSL-6; ADAMTSL6; FVSY9334;
                     PRO34005"
ORIGIN      
        1 gcaaactctg agccagagct ctccgcgcgc gcctgaaccg ctggccgccc gcgcagcccg
       61 acgcggaatc ggggcacagc gggagcccgt gcagggcggg cgcggggcgg ctgggcggcg
      121 gtggcggccg tccatgcggc ggcgctcggg gctgcccggc gccgggaacc acgcgggggc
      181 gaggcgaggc gaggcggccg ccggtcgctc cgggacgcgg accgccagga cttgaacgca
      241 actcccaatt gcagaaaatt ggcaacgtct ctgaagagcc cttgcttttg cctggacccc
      301 cagcatcatg gtttcccatt tcatggggtc tctcagtgtc ctgtgtttcc ttctgctgct
      361 tggattccag ttcgtctgcc cacagccctc cactcaacac aggaaggtcc cgcagcggat
      421 ggcggcggag ggcgcccccg aggacgacgg cggcggcggc gccccgggag tgtggggcgc
      481 ctggggcccc tggtcggcct gctcgcgtag ctgcagcggc ggcgtgatgg agcagacgcg
      541 gccctgcctg ccccgctcct accgcctgcg cggcggccag cggcctggcg cccctgcgcg
      601 cgccttcgcg gaccacgtgg tgtcggcggt gcgcacgtcg gtgccactgc accggagccg
      661 cgacgagacg ccagcgctgg ccggtacgga cgccagccgc cagggcccca cggtgctgcg
      721 aggcagccgg cacccacagc cccagggcct cgaagtcact ggggacagaa ggagcaggac
      781 ccgtggtacc attggccctg gcaagtatgg ctatggtaag gccccatata tcttaccact
      841 gcagacagac actgcacaca cgccacagag gctccggaga cagaagctct catcccgcca
      901 ttccaggtcc cagggagcat cttctgctag gcatggctac agttcaccag cccaccaggt
      961 cccccaacat gggcctttgt accaaagtga cagtggccct cgctctggac tgcaggctgc
     1021 ggaggccccc atctaccagc tacctttgac ccatgatcaa ggctaccctg cagcttcaag
     1081 tctctttcac agcccagaaa caagcaacaa ccacggtgtg gggacccatg gggcaactca
     1141 gagcttctct cagcctgccc gatctacagc aatctcatgc atcggggcct atcggcagta
     1201 caagctgtgc aacaccaacg tatgtccaga aagcagtaga agtatccggg aggtacagtg
     1261 tgcatcctac aacaacaagc cattcatggg ccggttttat gagtgggaac catttgcaga
     1321 agtaaaaggc aatcgcaaat gtgagttgaa ctgccaggca atgggctacc gcttctatgt
     1381 acggcaagct gagaaagtca tcgatggcac cccctgtgac cagaacggca cggccatctg
     1441 tgtgtctggg cagtgcaaga gcattggctg tgatgactac ttaggctccg acaaagtcgt
     1501 ggacaaatgt ggggtgtgtg gaggagacaa cacgggctgt caggttgtgt cgggcgtgtt
     1561 taagcatgcc ctcaccagcc tgggctacca ccgcgtcgtg gagattcccg agggagccac
     1621 gaaaatcaac atcacggaga tgtacaagag caacaactat ttggccctga gaagtcgttc
     1681 tggacgctcc atcatcaatg ggaactgggc aattgatcga ccaggaaaat acgagggcgg
     1741 agggaccatg ttcacctaca agcgtccaaa tgagatttcg agcactgccg gagagtcctt
     1801 tttggcggaa ggtcccacca acgagatctt ggatgtctac atgatacacc agcagccaaa
     1861 cccaggcgtg cactacgagt acgtgatcat ggggaccaac gccatcagcc cccaggtgcc
     1921 accccacagg agaccagggg aacccttcaa tggccagatg gtgacagaag gcaggagcca
     1981 ggaggaggga gaacagaaag ggaggaacga ggagaaggaa gacttgcgtg gggaggcccc
     2041 tgagatgttc acctcagaat cggcacagac cttcccagtc aggcatccag acagattttc
     2101 tccccatcga ccggacaact tggtgccacc agcaccgcag cccccacggc gcagccggga
     2161 tcacaactgg aagcagcttg ggacaacaga atgttccacg acctgtggga aaggatcgca
     2221 gtaccctatt ttccgctgtg tgcacagaag cactcatgaa gaggctcctg agagttactg
     2281 tgactccagc atgaagccga cccccgagga ggagccctgc aacatcttcc cttgcccagc
     2341 cttctgggac atcggggagt ggtctgagtg cagcaagacc tgtggcctgg gcatgcagca
     2401 ccgccaggtt ctgtgccgcc aggtgtacgc caaccgcagc ctgacggtgc agccctaccg
     2461 ctgccagcac ctggagaaac ctgagaccac cagcacctgc caactcaaga tctgcagcga
     2521 gtggcagatc cggaccgact ggacctcgtg ctcggtgccc tgcggcgtgg gacagaggac
     2581 ccgtgatgtg aagtgtgtga gcaacattgg ggatgtggtt gacgatgagg aatgcaacat
     2641 gaagctccgg ccgaatgaca ttgagaactg cgacatggga ccctgtgcca agagctggtt
     2701 cctcaccgag tggagcgaaa ggtgctcagc ggagtgtggg gccggagtgc ggacacgctc
     2761 ggtggtgtgc atgaccaacc atgtcagcag cctgcccctg gagggctgtg ggaacaaccg
     2821 gccggcagag gccaccccat gtgacaacgg accctgcacg ggcaaggtgg agtggtttgc
     2881 cgggagctgg agtcagtgtt ccatcgagtg tgggagcggg acgcaacaga gggaggtgat
     2941 ttgtgttaga aagaatgcag acacctttga agtgttggac ccctctgaat gttctttcct
     3001 ggagaaaccc cccagccagc aatcctgcca cctcaagcct tgcggagcca aatggtttag
     3061 caccgaatgg agcatgtgtt ccaagagctg ccagggtggc tttcgggtcc gggaagtgcg
     3121 gtgtctgtct gatgacatga ctctaagtaa cctctgtgac cctcagttga aaccagaaga
     3181 gagagaatct tgtaaccctc aggactgtgt ccctgaagtt gatgaaaact gcaaggacaa
     3241 gtactacaac tgcaacgtgg tggtccaggc aagactctgt gtctacaact actacaagac
     3301 cgcctgctgt gcctcctgca cccgtgtggc caacaggcag acgggcttcc tggggagcag
     3361 ataacactcc tgcaccccca tcagtagggc agcatcactg ccttcccggg ggcttcagca
     3421 gtgcgcctgg ctggctgctg ctccaccacg ggccccctgg cccaggcgct gccaaccaac
     3481 ttagtcacca cccctgcctc cggtgaatgc accccgtggt acccaggggc tttttacaca
     3541 agatgtttga aagccacagt cagtccttta agcatcacca tgtactgatg atcccctcct
     3601 tggacctggc atctgctaat ggtgcccttt gaaagtcaag cagtgggaag tacatggagc
     3661 tctcagccct gctcccatct ggcaccttca agtcagcaga tgggccactg actgagcact
     3721 gccccgtccc tggtgctact ggtctttcta aacttagcac cctggagagt ccaaggaggc
     3781 agcgccccca acccagcgcc ccactaagcc ttgctgacac gcgtgcatcc ctctgtgacc
     3841 tcagcccaga tgtgcctgtt ttcattctca aagacattag actgttttcc tgccctatga
     3901 cacagatagc tcacatgaat attgtgcttt atttagcagg tgtactcaca gatactagct
     3961 ccttagcagc tcacaacatc ccagaatggg aggcaggggg tgactcatta tccccatttt
     4021 actgacaggg aaactgaggc tcaacttaag taattgacct gccaggtata ttcacccatc
     4081 cagtggaaga gctgagtccc cgccccagtc atctaccagt atccagcctg gggcctgtac
     4141 ttagatgtga aaggtgctgc ttcatttctg accaagagac tgagaagttt cccagaatgc
     4201 aaacaaagcc caggcccctg aaatctttcc ggtcaagcct ttatcccagc actcagttgt
     4261 tttggatgtc tgttcctact tgcccttacc cccaaagtta cagatcctag ttacaggact
     4321 ctgccagctt tgttaaactg tccgtgagac aagaaagcca ttggggaaac caggtgattg
     4381 cctgaaattc ttactccgtt ccaagtgctg ttcctcccag gaaatcaaag gccagggtcc
     4441 ttatggccgt ggagccttcc cgaccacaga gccaacttgt gaagcacaca gctctgcagc
     4501 ctgggctctg ccctgcctca gccgcctccc ccacgctctt caccacgttc ctggagagtc
     4561 cggccaacct gtcccagccg aaacactgct gtattagaaa aagtctcttt ctggtctttc
     4621 tggttttgtt tatgaatttc cctctgtggc cacaaattcc tcccctcccc catgactcac
     4681 agtccatatg gcccaccccc agacttgagc accaagctct gcattaatgc agttggcctg
     4741 cgacaaggag ctgtggaccc ttccccatct cttccaattc actttcccca actatccagt
     4801 tccagaggcc gcaggcctgg aaggatgcag tgcatattga aaggtggacc ctctgaaaac
     4861 agttaagagg aatatatgta tgttttaccc attaagaaaa aatggcaagc taaacaaatg
     4921 ttaaacttac agaaaatttg tcttatggtc ctgagcatat ttccctttta gagcaagcct
     4981 ggattcttag caaagtgttt cccccatttg ctcttttagc tgacaaatct gccactgtga
     5041 tgatggtttg cagcttttgg aagcagtatg gcaacctggc ctgacatgct ctttaggctt
     5101 ccactaacct ggggctttca gaaattctat ttggcctttc tgtgggtagc tttccagctt
     5161 ctcttctagg gagccccagg catcatttcc caaaagcatc cccatctcct gattctcttg
     5221 gaactcctac agataagcat cctggcagag gcccaggctc ccaaaccgac aaagtgaaaa
     5281 gagaccagag aggccaagca tattgactgg tgctgttcag ggcctgctct tttccactca
     5341 ccacttgttt tgctgcttgt cacgaggaga gttgttcctg tatgtggctg ctctcagatc
     5401 tttccaagca agccagtcat ttgaagaggt tttcttttca tgctggaggg caggctaaga
     5461 tcaatgagtg gaagagagaa aggctgtttt agctcaagtt aaaggaacac cttctagcca
     5521 tcaaagccgc ccaacagagg caagggccac cacacatgag agagcgctct gtccttaaag
     5581 ggaattctct gttgagtggg aggtgaacac cctggttctt ccaactcagg aattctcgtg
     5641 gctgggctgg gtcagtgatg gctttgtctc tttatgtcta aagtgcccta tggctgctga
     5701 aggttaccta accattcttt aaaaggagaa tgaccctcca tgggaatggc cagcctgcca
     5761 actgtgcaat tgaagaagac ccgatggatc aaccccatgt ctcccttggg gagaaagtgc
     5821 ataaaccagg ggtctctttt tttttttcaa caaaccattg agctgttctt ggagttcatc
     5881 tctggagagg ttatacatta ttagaagttt gattattatt atagtttgat caatttattt
     5941 gtcttagaga tccaattttt actaattccc tagtttttta tttcagcatc tgaatgtctt
     6001 tctccctagc acagtgcata caatcagggc cttgggtatt tccagtgata actttccttg
     6061 gagaggatct aagaaaagcc cagatttcgg tagccatctc cctccaaata tgtctctttc
     6121 tgctttctta gtgcccatta tttccccctc tcctttcttc tgtcactgcc atctccttct
     6181 tggtcttccc attgttcttt aactggccgt aatgtggaat tgatatttac attttgatac
     6241 ggtttttttc ttggcctgtg tacgggattg cctcatttcc tgctctgaat tttaaaatta
     6301 gatattaaag ctgtcatatg gtttcctcac aaaagtcaac aaagtccaaa caaaaatagt
     6361 ttgccgtttt actttcatcc attgaaaaag gaaattgtgc ctcttgcagc ctaggcaaag
     6421 gacatttagt actatcgatt ctttccaccc tcacgatgac ttgcggttct ctctgtagaa
     6481 aagggatggc ctaagaaata caactaaaaa aacaaacaaa aacaccaaaa gaaaaaaaaa
     6541 agccatttaa agccagccac tagagggagt cagttcagtt ccgtaaaggt atgctcagtg
     6601 cccgctgcct gcaagctgtt ggggacccca gggagggcaa ggcagcctgt ccccgccccc
     6661 agggaactag aacatgacaa gaattctccg cactgtgcct acctgtccct ttaccttacc
     6721 tctctggccc agagttcttg gagggttttt tctttatttt cttatgtact catctactta
     6781 ttctcaaagt atttagcatt caacactctt tttgctttaa aaagaatggc cttacaaagg
     6841 gacagaaaag agaagacacg agcttggtgt attttcatca agttatgtgg cagagaaatc
     6901 cagatattac caggacctgt ctaaacaaat gttgtgggtt ttcttttcat tcggatagcc
     6961 actttatagt tggaatatca attctaatga ggaggaagac ataaatataa gtggtaaaaa
     7021 gaaacatgac ttcccttaaa acaggctgga taatctatat cagccttgtg ggtggagact
     7081 agtatttgat ccttgccata taaaacattt taatatggtt tacatgggaa aatatcgatg
     7141 gcttcctcac aaaatgtatg ggtgacgtga agttgaagag ccaatggctt gggtgacacg
     7201 tgctggatcc aaaaagatca gggagactag aataaaactt ggatgttaaa aattcaccag
     7261 gaatccacat aaagtactat atttgggcta aaatgaaaaa ctaaatacaa ggtgggagag
     7321 aggcaagaat ttcagttgac taagctcagt gtgagtcaaa gtgggatgga accatgcaaa
     7381 aacaaaaccc acagacatgc aggctacgtg aggagaaaac agtggtgagg atcacatcac
     7441 attgtgtttg catttgccgg aaccatactt taagaagaaa accgatcatc tataataaca
     7501 tcagtttatc aatgccccgt cctgatgaag tgtgcagact ctcagaaaca gcaggaagga
     7561 cttcatgaga accctcaggc tggagaaggc actagggcac aaggagagct ctcctaggac
     7621 caggaccaag aagctacagg caggcacagt ttagctcctg cagagaccca gcttttcaca
     7681 agttggagcc ttccagagat agagggactg tggtaggtgg tgacccaccc atcactggag
     7741 gtggaagcag aggccgtttg ccagggatgc tggagagggg attcaagcat ctggctgggc
     7801 aacgtgatgc tcagggccgt ctccactcag ggcttagggg agtctgtgag tagaagagct
     7861 ttaggtgatt tgtttggtgg gggaaggcaa gtacacagct atgcactttc cgtttctgac
     7921 ttttgccacc ctgtcagcca tggggagccc actgtgggac tgaaaccctg agctgaatgc
     7981 ggcctcatgt ctcagagaaa cactggcaag ttggtcagag ccgccgtctg catcgaggcg
     8041 tagctgagcg gcaggatggg gggctgcctg cccagggtct ctcaccgtgg tgtaagcaga
     8101 gccatggctt gcctaggacc ctatagatac catcactctt tctcagctcg actggagttt
     8161 ctgcaccttt gcaggggcaa agtaactccc tgcaccctga accacccccc attcctgttc
     8221 atttcagcag ataatgatgg aggggggggg tgtccatcgt gctgagggtg tgaccgcaag
     8281 agggtgaaaa cttccagcca actttctcag tcctttctct tgcgagaggg aagccacctg
     8341 ctatacaaac taataccccc tgccttgacc ccttccccac gactcagttg acagaaggat
     8401 atactttgtt ataacttatt attttgttct ctgtaaatac aagatgttta taggaaatat
     8461 gtattctgaa ctctatctgc agaatgagtc actacaccaa aatagttcta ttatttagaa
     8521 tgtgttaatt ttaaagggac ctgataggta tttatttaca tatgcgatcc acatttgtgt
     8581 gaaagcatgt gatcatacta acccagcctc ctggaatgtc gctgtacgat gattgatgtc
     8641 tttttctcag tccatagtta caattgttta gtatgctaat cagtccagtt ccctgaggtt
     8701 taagatcaaa tataaattac tctgcttttc gactcattca ggtagcattg tacctgaacc
     8761 tgattgctac tttttcatct taaatattat atttcctcat ctaatctgcc ttcccctcat
     8821 ccacagacat ttggagaagg aaatgggagg gtgtctgtta tccctttctc tttgctttgt
     8881 ccccgttgtt agactggcag cgtcagttgc tcggtgggct tggttagagc cgtgggtgag
     8941 gcaggtggct ggcggggaca gggagaggct gagagggaag tggtggcatt tactgctctg
     9001 acacttccac tgtccctgct ggggatgctg gggccaaggc ctgtggggcc tgtgaactgc
     9061 acagccagga gcaaggaacc cactaaatac tccgtcacct ccatgtcccc tctacagtgt
     9121 taaattatta cataagcagg tgaaaggtag aaggcgaatt atgtgagtaa atatggtctg
     9181 ttttctcttc agcaaaaatg actatttttg tgtgtgacta atttattttt attattgtaa
     9241 agatacaata aaccggttga aatatctgct ttgttgacaa gcgtgtgctt tctctggcct
     9301 tattcgcgtt ctgttctcct gcaaatagcg ccctctaaaa agaagagtca gacaataaac
     9361 tggttgaaat atc
//
