LOCUS       NM_032361               1601 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens THO complex subunit 3 (THOC3), transcript variant 1,
            mRNA.
ACCESSION   NM_032361 XM_942099
VERSION     NM_032361.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Yu,T., Zhang,Q., Yu,S.K., Nie,F.Q., Zhang,M.L., Wang,Q. and Lu,K.H.
  TITLE     THOC3 interacts with YBX1 to promote lung squamous cell carcinoma
            progression through PFKFB4 mRNA modification
  JOURNAL   Cell Death Dis 14 (7), 475 (2023)
   PUBMED   37500615
  REMARK    GeneRIF: THOC3 interacts with YBX1 to promote lung squamous cell
            carcinoma progression through PFKFB4 mRNA modification.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Chi,B., Wang,Q., Wu,G., Tan,M., Wang,L., Shi,M., Chang,X. and
            Cheng,H.
  TITLE     Aly and THO are required for assembly of the human TREX complex and
            association of TREX components with the spliced mRNA
  JOURNAL   Nucleic Acids Res 41 (2), 1294-1306 (2013)
   PUBMED   23222130
REFERENCE   3  (bases 1 to 1601)
  AUTHORS   Grolimund,L., Aeby,E., Hamelin,R., Armand,F., Chiappe,D.,
            Moniatte,M. and Lingner,J.
  TITLE     A quantitative telomeric chromatin isolation protocol identifies
            different telomeric states
  JOURNAL   Nat Commun 4, 2848 (2013)
   PUBMED   24270157
REFERENCE   4  (bases 1 to 1601)
  AUTHORS   Katahira,J.
  TITLE     mRNA export and the TREX complex
  JOURNAL   Biochim Biophys Acta 1819 (6), 507-513 (2012)
   PUBMED   22178508
  REMARK    Review article
REFERENCE   5  (bases 1 to 1601)
  AUTHORS   Boyne,J.R., Colgan,K.J. and Whitehouse,A.
  TITLE     Recruitment of the complete hTREX complex is required for Kaposi's
            sarcoma-associated herpesvirus intronless mRNA nuclear export and
            virus replication
  JOURNAL   PLoS Pathog 4 (10), e1000194 (2008)
   PUBMED   18974867
REFERENCE   6  (bases 1 to 1601)
  AUTHORS   Cheng,H., Dufu,K., Lee,C.S., Hsu,J.L., Dias,A. and Reed,R.
  TITLE     Human mRNA export machinery recruited to the 5' end of mRNA
  JOURNAL   Cell 127 (7), 1389-1400 (2006)
   PUBMED   17190602
REFERENCE   7  (bases 1 to 1601)
  AUTHORS   Masuda,S., Das,R., Cheng,H., Hurt,E., Dorman,N. and Reed,R.
  TITLE     Recruitment of the human TREX complex to mRNA during splicing
  JOURNAL   Genes Dev 19 (13), 1512-1517 (2005)
   PUBMED   15998806
  REMARK    GeneRIF: recruitment of the human TREX complex to spliced mRNA is
            not directly coupled to transcription, but is instead coupled to
            transcription indirectly through splicing
REFERENCE   8  (bases 1 to 1601)
  AUTHORS   Guo,S., Hakimi,M.A., Baillat,D., Chen,X., Farber,M.J.,
            Klein-Szanto,A.J., Cooch,N.S., Godwin,A.K. and Shiekhattar,R.
  TITLE     Linking transcriptional elongation and messenger RNA export to
            metastatic breast cancers
  JOURNAL   Cancer Res 65 (8), 3011-3016 (2005)
   PUBMED   15833825
REFERENCE   9  (bases 1 to 1601)
  AUTHORS   Strasser,K., Masuda,S., Mason,P., Pfannstiel,J., Oppizzi,M.,
            Rodriguez-Navarro,S., Rondon,A.G., Aguilera,A., Struhl,K., Reed,R.
            and Hurt,E.
  TITLE     TREX is a conserved complex coupling transcription with messenger
            RNA export
  JOURNAL   Nature 417 (6886), 304-308 (2002)
   PUBMED   11979277
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC138965.2.
            
            On Nov 22, 2019 this sequence version replaced NM_032361.3.
            
            Summary: This gene encodes a component of the nuclear THO
            transcription elongation complex, which is part of the larger
            transcription export (TREX) complex that couples messenger RNA
            processing and export. In humans, the transcription export complex
            is recruited to the 5'-end of messenger RNAs in a splicing- and
            cap-dependent manner. Studies of a related complex in mouse suggest
            that the metazoan transcription export complex is involved in cell
            differentiation and development. A pseudogene of this gene has been
            defined on chromosome 5. [provided by RefSeq, May 2013].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006849.1, SRR1163657.215830.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149004, SAMEA2156670
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000265097.9/
                                                ENSP00000265097.5
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-374               AC138965.2         151369-151742       c
            375-531             AC138965.2         150538-150694       c
            532-736             AC138965.2         148378-148582       c
            737-898             AC138965.2         144700-144861       c
            899-999             AC138965.2         144478-144578       c
            1000-1601           AC138965.2         142958-143559       c
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.2"
     gene            1..1601
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /note="THO complex subunit 3"
                     /db_xref="GeneID:84321"
                     /db_xref="HGNC:HGNC:19072"
                     /db_xref="MIM:606929"
     exon            1..374
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..1163
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /note="isoform 1 is encoded by transcript variant 1; TEX1
                     homolog; THO complex 3"
                     /codon_start=1
                     /product="THO complex subunit 3 isoform 1"
                     /protein_id="NP_115737.1"
                     /db_xref="CCDS:CCDS4397.1"
                     /db_xref="GeneID:84321"
                     /db_xref="HGNC:HGNC:19072"
                     /db_xref="MIM:606929"
                     /translation="MAVPAAAMGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRG
                     HSKTREFLAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEKDRLVKENNYRGHGDSV
                     DQLCWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGN
                     KDDVVTFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQ
                     SINAHPSNCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSH
                     DGKMLASASEDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYD
                     SSREAGTVKLFGLPNDS"
     exon            375..531
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     exon            532..736
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     exon            737..898
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     exon            899..999
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1601
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1575..1580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /note="hexamer: AATAAA"
     polyA_site      1601
                     /gene="THOC3"
                     /gene_synonym="hTREX45; THO3"
                     /note="major polyA site"
ORIGIN      
        1 agaggggccg tcgctcttcc gggcgcatgc gtgcggcagc ggcgccagga ctgactgcgc
       61 cgtggaggct gctgcagtgt tgtgagttgg aagctgggga gctcggcatg gcggtccccg
      121 ctgcagccat ggggccctcg gcgttgggcc agagcggccc cggctcgatg gccccgtggt
      181 gctcagtgag cagcggcccg tcgcgctacg tgcttgggat gcaggagctg ttccggggcc
      241 acagcaagac gcgcgagttc ctggcgcaca gcgccaaggt gcactcggtg gcctggagtt
      301 gcgacgggcg tcgcctagcc tcggggtcct tcgacaagac ggccagcgtc ttcttgctgg
      361 agaaggaccg gttggtcaaa gaaaacaatt atcggggaca tggggatagt gtggaccagc
      421 tttgttggca tccaagtaat cctgacctat ttgttacggc gtccggagat aaaaccattc
      481 gcatctggga tgtgaggact acaaaatgca ttgccactgt gaacactaaa ggggagaaca
      541 ttaatatctg ctggagtcct gatgggcaga ccattgctgt aggcaacaag gatgatgtgg
      601 tgacctttat tgatgccaag acacaccgtt ccaaagcaga agagcagttc aagttcgagg
      661 tcaacgaaat ctcctggaac aatgacaata atatgttctt cctgacaaat ggcaatggtt
      721 gtatcaacat cctcagctac ccagaactga agcctgtgca gtccatcaac gcccatcctt
      781 ccaactgcat ctgtatcaag tttgacccca tggggaagta ctttgccaca ggaagtgcag
      841 atgctttggt cagcctctgg gatgtggatg agttagtgtg tgttcggtgc ttttccaggc
      901 tggattggcc tgtaagaacc ctcagtttca gccatgatgg gaaaatgctg gcgtcagcat
      961 cggaagatca ttttattgac attgctgaag tggagacagg ggacaaacta tgggaggtac
     1021 agtgtgagtc tccgaccttc acagtggcgt ggcaccccaa aaggcctctg ctggcatttg
     1081 cctgtgatga caaagacggc aaatatgaca gcagccggga agccggaact gtgaagctgt
     1141 ttgggcttcc taatgattct tgagaggagg ttgtagggag aggaggcccc ggcagaggtc
     1201 ttccttcatg tggttagttt ggtctgttct ctcggagttg gtgggcaccc taaatatttg
     1261 taagttggta taaattgtaa acgtctctgg tcaggctgcg catttcattc ttttgctttg
     1321 tctgtgtatt agctctttcc attctttgcc cccagcatga gttaactcgc gtggactctg
     1381 cagtgcgagt agtgacccca ccataccttg tcctctggac ctcctgtctt ctctgcttct
     1441 gggtgcatgg tagactttgt ggcatttgat acaacttgga caatacctag tttggaggga
     1501 ggggaatgga agggcatgga agttttttta aataattaaa aatatatata tataattttg
     1561 agaattgagc atttaataaa ctgacttttg ttattatgga a
//
