LOCUS       NM_001081550            5600 bp    mRNA    linear   PRI 18-OCT-2024
DEFINITION  Homo sapiens THO complex subunit 2 (THOC2), mRNA.
ACCESSION   NM_001081550
VERSION     NM_001081550.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Zhou,X., Liu,X., Zhang,G., Zhang,Q., Chen,H., Wang,Y., Fang,F. and
            Sun,J.
  TITLE     Knockdown THOC2 suppresses the proliferation and invasion of
            melanoma
  JOURNAL   Bioengineered 10 (1), 635-645 (2019)
   PUBMED   31680623
  REMARK    GeneRIF: THOC2 reduction repressed melanoma cell proliferation and
            invasion, and induced cell apoptosis.
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Kumar,R., Gardner,A., Homan,C.C., Douglas,E., Mefford,H.,
            Wieczorek,D., Ludecke,H.J., Stark,Z., Sadedin,S., Nowak,C.B.,
            Douglas,J., Parsons,G., Mark,P., Loidi,L., Herman,G.E., Mihalic
            Mosher,T., Gillespie,M.K., Brady,L., Tarnopolsky,M., Madrigal,I.,
            Eiris,J., Domenech Salgado,L., Rabionet,R., Strom,T.M.,
            Ishihara,N., Inagaki,H., Kurahashi,H., Dudding-Byth,T.,
            Palmer,E.E., Field,M. and Gecz,J.
  CONSRTM   Broad CMG
  TITLE     Severe neurocognitive and growth disorders due to variation in
            THOC2, an essential component of nuclear mRNA export machinery
  JOURNAL   Hum Mutat 39 (8), 1126-1138 (2018)
   PUBMED   29851191
  REMARK    GeneRIF: Study presents detailed clinical assessment and functional
            studies on a de novo variant in a female with an epileptic
            encephalopathy and discuss an additional four families with rare
            variants in THOC2 with supportive evidence for pathogenicity.
REFERENCE   3  (bases 1 to 5600)
  AUTHORS   Kumar,R., Corbett,M.A., van Bon,B.W., Woenig,J.A., Weir,L.,
            Douglas,E., Friend,K.L., Gardner,A., Shaw,M., Jolly,L.A., Tan,C.,
            Hunter,M.F., Hackett,A., Field,M., Palmer,E.E., Leffler,M.,
            Rogers,C., Boyle,J., Bienek,M., Jensen,C., Van Buggenhout,G., Van
            Esch,H., Hoffmann,K., Raynaud,M., Zhao,H., Reed,R., Hu,H.,
            Haas,S.A., Haan,E., Kalscheuer,V.M. and Gecz,J.
  TITLE     THOC2 Mutations Implicate mRNA-Export Pathway in X-Linked
            Intellectual Disability
  JOURNAL   Am J Hum Genet 97 (2), 302-310 (2015)
   PUBMED   26166480
  REMARK    GeneRIF: THOC2 mutations implicate mRNA-export pathway in X-linked
            intellectual disability.
REFERENCE   4  (bases 1 to 5600)
  AUTHORS   Grolimund,L., Aeby,E., Hamelin,R., Armand,F., Chiappe,D.,
            Moniatte,M. and Lingner,J.
  TITLE     A quantitative telomeric chromatin isolation protocol identifies
            different telomeric states
  JOURNAL   Nat Commun 4, 2848 (2013)
   PUBMED   24270157
REFERENCE   5  (bases 1 to 5600)
  AUTHORS   Folco,E.G., Lee,C.S., Dufu,K., Yamazaki,T. and Reed,R.
  TITLE     The proteins PDIP3 and ZC11A associate with the human TREX complex
            in an ATP-dependent manner and function in mRNA export
  JOURNAL   PLoS One 7 (8), e43804 (2012)
   PUBMED   22928037
REFERENCE   6  (bases 1 to 5600)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 5600)
  AUTHORS   Strasser,K., Masuda,S., Mason,P., Pfannstiel,J., Oppizzi,M.,
            Rodriguez-Navarro,S., Rondon,A.G., Aguilera,A., Struhl,K., Reed,R.
            and Hurt,E.
  TITLE     TREX is a conserved complex coupling transcription with messenger
            RNA export
  JOURNAL   Nature 417 (6886), 304-308 (2002)
   PUBMED   11979277
REFERENCE   8  (bases 1 to 5600)
  AUTHORS   Coffey,A.J., Brooksbank,R.A., Brandau,O., Oohashi,T., Howell,G.R.,
            Bye,J.M., Cahn,A.P., Durham,J., Heath,P., Wray,P., Pavitt,R.,
            Wilkinson,J., Leversha,M., Huckle,E., Shaw-Smith,C.J., Dunham,A.,
            Rhodes,S., Schuster,V., Porta,G., Yin,L., Serafini,P., Sylla,B.,
            Zollo,M., Franco,B., Bolino,A., Seri,M., Lanyi,A., Davis,J.R.,
            Webster,D., Harris,A., Lenoir,G., de St Basile,G., Jones,A.,
            Behloradsky,B.H., Achatz,H., Murken,J., Fassler,R., Sumegi,J.,
            Romeo,G., Vaudin,M., Ross,M.T., Meindl,A. and Bentley,D.R.
  TITLE     Host response to EBV infection in X-linked lymphoproliferative
            disease results from mutations in an SH2-domain encoding gene
  JOURNAL   Nat Genet 20 (2), 129-135 (1998)
   PUBMED   9771704
REFERENCE   9  (bases 1 to 5600)
  AUTHORS   Gu,X.X., Decorte,R., Marynen,P., Fryns,J.P., Cassiman,J.J. and
            Raeymaekers,P.
  TITLE     Localisation of a new gene for non-specific mental retardation to
            Xq22-q26 (MRX35)
  JOURNAL   J Med Genet 33 (1), 52-55 (1996)
   PUBMED   8825049
REFERENCE   10 (bases 1 to 5600)
  AUTHORS   Mulley,J.C., Kerr,B., Stevenson,R. and Lubs,H.
  TITLE     Nomenclature guidelines for X-linked mental retardation
  JOURNAL   Am J Med Genet 43 (1-2), 383-391 (1992)
   PUBMED   1605216
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA504931.1, AW206982.1,
            AF441770.1 and BC054050.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001081550.1.
            
            Summary: The TREX multiprotein complex binds specifically to
            spliced mRNAs to facilitate mRNA export. The protein encoded by
            this gene is a member of the THO complex, a subset of the TREX
            complex. The encoded protein interacts with the THOC1
            protein.[provided by RefSeq, Jun 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.3428918.1,
                                           SRR1660807.247482.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245838.13/ ENSP00000245838.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-375               DA504931.1         3-377
            376-557             AW206982.1         342-523
            558-4827            AF441770.1         183-4452
            4828-5600           BC054050.1         504-1276
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq25"
     gene            1..5600
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /note="THO complex subunit 2"
                     /db_xref="GeneID:57187"
                     /db_xref="HGNC:HGNC:19073"
                     /db_xref="MIM:300395"
     exon            1..101
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..4812
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /note="THO complex 2"
                     /codon_start=1
                     /product="THO complex subunit 2"
                     /protein_id="NP_001075019.1"
                     /db_xref="CCDS:CCDS43988.1"
                     /db_xref="GeneID:57187"
                     /db_xref="HGNC:HGNC:19073"
                     /db_xref="MIM:300395"
                     /translation="MAAAAVVVPAEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRD
                     FQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKS
                     KRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQ
                     KFNLLREENEGYAKLIAELGQDLSGSITSDLILENIKSLIGCFNLDPNRVLDVILEVF
                     ECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQF
                     NLIDLDDLYVHLLPADNCIMDEHKREIAEAKQIVRKLTMVVLSSEKMDEREKEKEKEE
                     EKVEKPPDNQKLGLLEALLKIGDWQHAQNIMDQMPPYYAASHKLIALAICKLIHITIE
                     PLYRRVGVPKGAKGSPVNALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFA
                     KVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEEL
                     WGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGR
                     QIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPE
                     KERMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEV
                     VQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLAL
                     PLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVP
                     SIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMV
                     MAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKVQMKAID
                     DNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNE
                     TITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVAS
                     CTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQL
                     DYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALER
                     RVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGP
                     GGGPSSSSIGSASKSDESSTEETDKSRERSQCGVKAVNKASSTTPKGNSSNGNSGSNS
                     NKAVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKER
                     TPKSDKEKEKFKKEEKAKDEKFKTTVPNAESKSTQEREREKEPSRERDIAKEMKSKEN
                     VKGGEKTPVSGSLKSPVPRSDIPEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSA
                     KLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPP
                     DLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGSDSFKSEKMDK
                     ISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR"
     exon            102..160
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            161..252
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            253..304
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            305..375
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            376..497
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            498..631
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            632..798
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            799..891
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1047
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1220
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1416
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1458
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1589
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1691
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1776
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1870
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1951
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2048
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2166
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2346
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2511
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2787
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2929
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            2930..3087
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3216
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3217..3348
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3349..3533
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3712
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3713..3815
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            3816..4246
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4247..4305
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4306..4341
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4342..4479
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4480..4549
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4550..4707
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4708..4784
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4785..4830
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     exon            4831..5600
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5579..5584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /note="hexamer: AATAAA"
     polyA_site      5600
                     /gene="THOC2"
                     /gene_synonym="AMC7; CXorf3; dJ506G2.1; hTREX120; MRX12;
                     MRX35; THO2; XLID12"
                     /note="major polyA site"
ORIGIN      
        1 atccgggctt ctgctactag tgagaggaag atggcggccg cggctgtggt ggttcccgca
       61 gagtggataa agaactggga gaaatcaggg agaggcgaat ttttgcattt atgtcggatc
      121 ctcagtgaaa ataaaagcca tgatagttca acatacagag atttccagca agctctctat
      181 gagttgtcat atcatgtaat taaaggaaat ctaaagcatg aacaggcatc taatgttctt
      241 agtgacatta gtgaatttcg tgaggatatg ccctccattc ttgctgatgt attctgcata
      301 ttagacattg agacaaattg tttagaagaa aaaagcaaga gagactattt tacacagttg
      361 gtattagcat gtttgtattt agtttcagac acagttctaa aggaacgcct ggatccagaa
      421 acactggaat cattagggct tatcaaacaa tcacagcaat tcaatcaaaa gtcagttaaa
      481 atcaagacaa aactctttta taagcagcaa aaattcaatt tgttaagaga agagaatgaa
      541 ggttatgcca agctgattgc tgaattgggg caagatttat ctggaagtat tactagtgat
      601 ttaatcttag aaaatatcaa atctttaata ggatgcttta atctggatcc caatagagtt
      661 ttggatgtca ttttagaagt gtttgaatgc aggccagaac acgatgactt ctttatatct
      721 ttgttagaat cttacatgag tatgtgtgaa ccgcaaacac tgtgtcatat tcttgggttc
      781 aaattcaagt tttaccagga accaaatggc gagacaccat catctttata cagagttgca
      841 gcagtacttc tacaatttaa tcttattgat ttagatgatc tttatgtaca tcttcttccg
      901 gctgataatt gcattatgga tgaacacaaa cgagaaattg cggaagctaa gcaaattgtt
      961 agaaagctta cgatggttgt gttgtcttct gaaaaaatgg atgagcgaga gaaagaaaag
     1021 gaaaaagaag aggagaaagt agagaaacca cctgataacc aaaaacttgg cttgttggaa
     1081 gccttattaa agattggtga ttggcaacat gcacagaaca ttatggatca gatgcctcca
     1141 tactatgcag cttcacacaa gctaatagcc cttgctattt gcaagctcat tcatataact
     1201 attgagcctc tctaccgaag agttggagtt cctaaaggtg ctaaaggctc acctgttaat
     1261 gctttgcaaa acaagagagc accaaaacaa gctgagagct ttgaagattt gaggagagac
     1321 gtgttcaata tgttctgtta ccttggtcct cacctttctc acgatcccat tttatttgca
     1381 aaagtggtgc gcataggcaa gtcatttatg aaggagtttc agtctgatgg aagcaaacaa
     1441 gaagataaag aaaaaacgga agttatcctt agctgtttgc ttagcattac tgaccaggta
     1501 ctacttccat ctctttcttt gatggactgc aatgcttgta tgtctgagga actatgggga
     1561 atgtttaaaa catttccata tcagcataga tatcgtctgt atggccagtg gaagaatgaa
     1621 acttataaca gtcacccact tttagtaaaa gttaaagctc aaacaataga cagagccaaa
     1681 tatatcatga agcgcctaac caaggaaaat gtgaagcctt ctggaagaca aattgggaag
     1741 ttgagccaca gcaatccaac cattttgttt gattatatct tgtcacaaat acagaagtat
     1801 gataacttaa taacacctgt agtagattca ttgaaatacc tcacttcact gaattatgat
     1861 gtcttggcct attgtatcat tgaagcttta gctaatccag aaaaggaaag aatgaaacat
     1921 gatgacacaa ccatctcaag ctggcttcag agtctggcta gtttctgtgg tgcagttttt
     1981 cgtaaatatc caattgatct tgctggtctt cttcagtatg ttgccaatca gctaaaggcg
     2041 ggcaaaagtt ttgacctgct tatattgaaa gaagtggtac aaaaaatggc aggaatagaa
     2101 attacagagg aaatgacaat ggagcaacta gaggctatga ctggtggaga gcagctaaaa
     2161 gctgagggtg gttattttgg tcagatcaga aacactaaaa aatcctctca gagattaaag
     2221 gatgctctat tggaccatga tcttgccctt cctctctgtc tgcttatggc tcagcagaga
     2281 aatggggtaa tctttcagga aggtggagag aaacatttga aacttgtggg aaagctctat
     2341 gaccagtgtc atgataccct ggtgcagttt ggtgggtttt tagcatctaa tctgagcaca
     2401 gaagattata taaagcgagt gccttcaatt gatgtactct gtaatgaatt tcatacaccc
     2461 catgatgcag catttttcct gtctaggcca atgtatgccc atcatatttc gtcaaagtat
     2521 gatgaactta aaaaatcaga aaagggaagt aaacagcaac ataaagttca taagtacatt
     2581 acatcatgtg agatggtgat ggcgcctgtc catgaagcag tggtctcctt acatgtttcc
     2641 aaagtctggg atgacatcag ccctcaattc tatgctacat tctggtcatt gacaatgtat
     2701 gaccttgcag ttccacacac cagctatgaa cgagaagtca ataaacttaa agtccagatg
     2761 aaagcaattg atgacaatca ggaaatgccc ccaaataaaa agaaaaaaga gaaggagcgc
     2821 tgtactgccc ttcaggacaa gcttcttgaa gaagaaaaga aacagatgga acatgtacag
     2881 agagttctac agagattgaa actggaaaag gacaactggc ttttagcaaa atctaccaaa
     2941 aatgagacca tcacaaaatt tctacagctg tgtatatttc ctcgatgtat tttttcagca
     3001 attgatgctg tttactgtgc tcgttttgtt gaattggtac atcaacagaa aactccaaat
     3061 ttttccacac ttctttgcta tgatcgagtt ttctctgaca taatttacac agttgcaagc
     3121 tgtactgaaa atgaagccag tcgatacgga aggtttcttt gctgcatgtt agagactgtg
     3181 accaggtggc atagtgatag agccacatat gaaaaggaat gtggaaacta tccaggattc
     3241 cttaccatat tacgggcaac tggatttgat ggtggaaata aggctgatca attagactat
     3301 gaaaattttc gacatgttgt acataaatgg cattacaaac taaccaaggc atcggtacat
     3361 tgccttgaaa caggcgaata tactcacatc aggaatatct tgattgtgct aacaaaaata
     3421 cttccttggt acccaaaagt tttgaatctg ggtcaagctt tggaaagaag agtacacaaa
     3481 atctgccaag aagaaaaaga gaagaggcca gatctatatg cattggctat gggctactct
     3541 gggcagttga aaagtagaaa gtcatacatg atacctgaaa atgagtttca tcacaaagac
     3601 ccccctccga ggaatgcagt tgccagtgtg caaaatgggc ctggtggtgg gccttcttca
     3661 tcatcaatag gaagtgcatc taaatcggat gaaagcagta ctgaggagac tgataaatca
     3721 agggagagat ctcagtgtgg tgtgaaagct gttaataaag cttctagtac cacacctaaa
     3781 gggaattcaa gcaatggaaa tagtggctct aacagcaaca aagctgttaa agaaaatgac
     3841 aaagaaaaag ggaaagagaa agaaaaagag aaaaaagaaa agactccagc tactactcca
     3901 gaggccaggg tacttggtaa agatggtaaa gaaaaaccaa aggaagagcg gccaaataaa
     3961 gatgaaaaag caagagagac caaggaaaga acgccgaagt ctgacaaaga gaaagaaaaa
     4021 ttcaagaagg aagaaaaagc taaagatgag aaatttaaga ccactgtccc caacgcagaa
     4081 tcaaaatcaa ctcaagaaag ggaaagagag aaggagccat ccagagaaag agatatagca
     4141 aaggaaatga aatcaaagga aaatgttaaa ggaggagaaa aaacaccagt ttctgggtcc
     4201 ttgaaatcac ctgttcccag atcagatatt ccagagcctg aaagggaaca aaaacgccgc
     4261 aaaattgata ctcacccttc tccatcacat tcctccacag taaaggacag tctcatcgaa
     4321 ctcaaggaat cttcagcaaa gctctacatt aatcatactc ctccaccact gtccaagagt
     4381 aaggagagag aaatggacaa gaaagatttg gacaagtcaa gggaaagatc cagagaaaga
     4441 gagaaaaaag atgaaaagga caggaaagag cggaaaaggg atcactcaaa caacgaccgt
     4501 gaagtgccac cggacttaac caagagacgt aaagaggaga atggaacaat gggggtttca
     4561 aaacataaaa gtgaaagtcc ttgtgaatct ccttatccaa atgagaaaga caaggaaaaa
     4621 aataagtcaa aatcttcagg caaagaaaaa ggcagtgatt catttaaatc tgagaagatg
     4681 gataaaatct cctccggtgg caaaaaggag tccaggcatg ataaagaaaa gatagaaaag
     4741 aaagagaaac gggacagttc aggaggaaag gaagagaaga aacatcataa gtcctcggac
     4801 aagcacagat aatgaagact ttccatcaag gtgagatcgg actggaactg ttcggctgcg
     4861 accagaaatt tattttcctg agtaaattgc cgagaattaa gaatgaagag ggccatttgc
     4921 atctccttaa attattcagt tacctgcttt attgctccat gtggaaaact taaaattgtt
     4981 aagttgtgca ttactgtatt ttaacttgtt gcttagtttc tacatgttta ttttcagtaa
     5041 tggctgaaag tgttaactgt tccatacttt tagcacaatg tgctgcataa ggttacctgt
     5101 gtacagagtt ttactttaga ttaactaaat attgcctggg ttcagttttt atttccattc
     5161 tgaaatgctt cctttttatt gtttgaaact gaaaataaac aattgttgaa cccttttgat
     5221 tttacctcat tttaaaactg ttttaattta ttatttggct tgttcttaat attagtcact
     5281 aaaagcagtg ggagcattgt cttatgaaat gcttaggaat cattttatat agtacatgta
     5341 caacattaaa cgtgtttaaa aaagaaaaag gtaccagcga tcacttgtcc cttgccattt
     5401 tttcttgtaa ttatgttaga caaatcttgg cggcgggggg atcaaaacat aattgtttta
     5461 attctacagc tgtaggagct ttgtattgct gaactttcat ctggaaaagt ttcacagtga
     5521 catttttaaa agagaatttt tttatctgcc gaattctacc agtgtaacct tttttctaaa
     5581 taaacaatag ttttctcaaa
//
