LOCUS       NM_001394520            3815 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens thymocyte selection associated (THEMIS), transcript
            variant 5, mRNA.
ACCESSION   NM_001394520 XM_011535814
VERSION     NM_001394520.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Sun,C., Shou,P., Du,H., Hirabayashi,K., Chen,Y., Herring,L.E.,
            Ahn,S., Xu,Y., Suzuki,K., Li,G., Tsahouridis,O., Su,L., Savoldo,B.
            and Dotti,G.
  TITLE     THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated Priming of
            Chimeric Antigen Receptor-Redirected T Cells
  JOURNAL   Cancer Cell 37 (2), 216-225 (2020)
   PUBMED   32004441
  REMARK    GeneRIF: THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated
            Priming of Chimeric Antigen Receptor-Redirected T Cells
REFERENCE   3  (bases 1 to 3815)
  AUTHORS   Choi,S., Warzecha,C., Zvezdova,E., Lee,J., Argenty,J., Lesourne,R.,
            Aravind,L. and Love,P.E.
  TITLE     THEMIS enhances TCR signaling and enables positive selection by
            selective inhibition of the phosphatase SHP-1
  JOURNAL   Nat Immunol 18 (4), 433-441 (2017)
   PUBMED   28250424
  REMARK    GeneRIF: we found that THEMIS directly regulated the catalytic
            activity of the tyrosine phosphatase SHP-1.
            Erratum:[Nat Immunol. 2017 May 18;18(6):705. doi:
            10.1038/ni0617-705c.. PMID: 28518157]
REFERENCE   4  (bases 1 to 3815)
  AUTHORS   Kinosada,H., Yasunaga,J.I., Shimura,K., Miyazato,P., Onishi,C.,
            Iyoda,T., Inaba,K. and Matsuoka,M.
  TITLE     HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the
            Suppressive Signaling of Co-inhibitory Receptors
  JOURNAL   PLoS Pathog 13 (1), e1006120 (2017)
   PUBMED   28046066
  REMARK    Erratum:[PLoS Pathog. 2017 Feb 23;13(2):e1006228. doi:
            10.1371/journal.ppat.1006228.. PMID: 28231270]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3815)
  AUTHORS   Davies,J.L., Thompson,S., Kaur-Sandhu,H., Sawcer,S., Coles,A.,
            Ban,M. and Jones,J.
  TITLE     Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated
            with a Genotype that Is Protective against Multiple Sclerosis
  JOURNAL   PLoS One 11 (7), e0158327 (2016)
   PUBMED   27438997
  REMARK    GeneRIF: we have demonstrated mutually exclusive use of exon 1 and
            2 amongst 16 novel THEMIS isoforms. We also show inverse
            correlation between THEMIS expression in human CD4+ T-cells and
            dosage of the multiple sclerosis risk allele at rs13204742, driven
            by reduced expression of exon 1- containing isoforms
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3815)
  AUTHORS   Kakugawa,K., Yasuda,T., Miura,I., Kobayashi,A., Fukiage,H.,
            Satoh,R., Matsuda,M., Koseki,H., Wakana,S., Kawamoto,H. and
            Yoshida,H.
  TITLE     A novel gene essential for the development of single positive
            thymocytes
  JOURNAL   Mol Cell Biol 29 (18), 5128-5135 (2009)
   PUBMED   19620281
REFERENCE   7  (bases 1 to 3815)
  AUTHORS   Allen,P.M.
  TITLE     Themis imposes new law and order on positive selection
  JOURNAL   Nat Immunol 10 (8), 805-806 (2009)
   PUBMED   19621038
REFERENCE   8  (bases 1 to 3815)
  AUTHORS   Fu,G., Vallee,S., Rybakin,V., McGuire,M.V., Ampudia,J.,
            Brockmeyer,C., Salek,M., Fallen,P.R., Hoerter,J.A., Munshi,A.,
            Huang,Y.H., Hu,J., Fox,H.S., Sauer,K., Acuto,O. and Gascoigne,N.R.
  TITLE     Themis controls thymocyte selection through regulation of T cell
            antigen receptor-mediated signaling
  JOURNAL   Nat Immunol 10 (8), 848-856 (2009)
   PUBMED   19597499
  REMARK    GeneRIF: Functional analysis of the mouse gene by knockout, and
            comparison of the mouse and human proteins.
REFERENCE   9  (bases 1 to 3815)
  AUTHORS   Lesourne,R., Uehara,S., Lee,J., Song,K.D., Li,L., Pinkhasov,J.,
            Zhang,Y., Weng,N.P., Wildt,K.F., Wang,L., Bosselut,R. and Love,P.E.
  TITLE     Themis, a T cell-specific protein important for late thymocyte
            development
  JOURNAL   Nat Immunol 10 (8), 840-847 (2009)
   PUBMED   19597498
  REMARK    GeneRIF: Functional characterization of the homologous mouse gene.
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
REFERENCE   10 (bases 1 to 3815)
  AUTHORS   Johnson,A.L., Aravind,L., Shulzhenko,N., Morgun,A., Choi,S.Y.,
            Crockford,T.L., Lambe,T., Domaschenz,H., Kucharska,E.M., Zheng,L.,
            Vinuesa,C.G., Lenardo,M.J., Goodnow,C.C., Cornall,R.J. and
            Schwartz,R.H.
  TITLE     Themis is a member of a new metazoan gene family and is required
            for the completion of thymocyte positive selection
  JOURNAL   Nat Immunol 10 (8), 831-839 (2009)
   PUBMED   19597497
  REMARK    Erratum:[Nat Immunol. 2010 Jan;11(1):97]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035470.10, AL365224.8 and
            AL356432.17.
            
            On Apr 23, 2021 this sequence version replaced XM_011535814.2.
            
            Summary: This gene encodes a protein that plays a regulatory role
            in both positive and negative T-cell selection during late
            thymocyte development. The protein functions through T-cell antigen
            receptor signaling, and is necessary for proper lineage commitment
            and maturation of T-cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AL035470.10        76128-76295
            169-327             AL365224.8         32192-32350         c
            328-354             AL365224.8         19223-19249         c
            355-813             AL365224.8         6638-7096           c
            814-1862            AL356432.17        160081-161129       c
            1863-1998           AL356432.17        66886-67021         c
            1999-3815           AL356432.17        55398-57214         c
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.33"
     gene            1..3815
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="thymocyte selection associated"
                     /db_xref="GeneID:387357"
                     /db_xref="HGNC:HGNC:21569"
                     /db_xref="MIM:613607"
     exon            1..168
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            169..327
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2030
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="isoform 5 is encoded by transcript variant 5;
                     thymocyte selection pathway associated; GRB2-associated
                     protein; signaling phosphoprotein specific for T cells;
                     thymocyte-expressed molecule involved in selection;
                     protein THEMIS"
                     /codon_start=1
                     /product="protein THEMIS isoform 5"
                     /protein_id="NP_001381449.1"
                     /db_xref="GeneID:387357"
                     /db_xref="HGNC:HGNC:21569"
                     /db_xref="MIM:613607"
                     /translation="MFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELP
                     MNFPGTFVKRSKECLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENL
                     IIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKE
                     IVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRI
                     LPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQ
                     PGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKS
                     EKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILK
                     KSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAAT
                     PGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEE
                     ITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDI
                     TKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK"
     exon            328..354
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            355..813
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            814..1862
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..1998
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..3815
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3796..3801
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="hexamer: AATAAA"
     polyA_site      3815
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="major polyA site"
ORIGIN      
        1 acttacaatt acgagattta tatttgcatt agtctctttg gctggtgggt aggggtgaga
       61 ggctcttcct ggatccctta ttttctacag gagaggagga aaacacctgg gatgctccag
      121 tgctcttacg cagataatga tcattaacat cagcctctct gatcaaaggc tctatttatg
      181 aaatgtttgg aaatgaatgc tgtttttcaa caggagaagt gattaaaatt actggtctca
      241 aagttaagaa gatcatagct gaaatttgtg agcagattga aggttgtgag tctctacagc
      301 catttgaact gcctatgaat tttccaggga cttttgttaa aagaagcaag gaatgtcttt
      361 ttaagattgt ggctgataaa actccatacc ttactatgga agaaatcaca aggaccattc
      421 atattggacc aagtagacta gggcatcctt gcttctatca tcagaaggat ataaaactag
      481 agaacctcat cataaagcag ggtgagcaaa tcatgctcaa ctcagttgaa gagattgatg
      541 gagaaataat ggtgagctgt gcagtagcaa ggaatcatca aactcactca tttaatttgc
      601 ctttgtcaca agaaggagaa ttctacgagt gtgaagatga acgtatttac actctaaagg
      661 agattgttga atggaagatt cctaagaaca gaacaagaac tgtaaacctt acagattttt
      721 caaataagtg ggactcaacg aatccatttc ctaaagactt ttatggtacc ctgattctca
      781 agcctgttta tgaaattcaa ggtgtgatga aatttcgaaa agatataatc cgcatcctcc
      841 ccagtctaga tgtcgaagtc aaagacatca ctgattctta cgatgctaac tggtttcttc
      901 agctgttatc aacagaagat ctttttgaaa tgactagtaa agagttcccc atagtgactg
      961 aagtcataga agcacctgaa ggaaaccacc tgccccaaag cattttacag cctgggaaaa
     1021 ccattgtgat ccacaaaaag taccaggcat caagaatctt agcttcagaa attagaagca
     1081 attttcctaa aagacacttc ttgatcccca ctagctataa aggcaagttc aagcggcgac
     1141 cgagggagtt cccaacggcc tatgacctag agatcgctaa gagtgaaaag gagcctcttc
     1201 acgtggtggc caccaaagcg tttcattccc ctcatgacaa gctgtcatcc gtatctgttg
     1261 gggaccagtt tctggtgcat cagtcagaga cgactgaagt cctctgtgag ggaataaaaa
     1321 aagtggtgaa tgttctggcc tgtgaaaaaa tcctcaaaaa gtcctatgag gctgcgctgc
     1381 tccctttgta catggaagga ggttttgtag aggtgattca tgataagaaa cagtacccga
     1441 tttctgagct ctgtaaacag ttccgtttgc ccttcaatgt gaaggtgtct gtcagggatc
     1501 tttccattga agaggacgtg ttggctgcca caccaggact gcagttggag gaggacatta
     1561 cagactctta cctactcata agtgactttg ccaaccccac ggagtgctgg gaaattcctg
     1621 tgggccgctt gaatatgact gttcagttag ttagtaattt ctctagggat gcagaaccat
     1681 ttctagtcag gactctggta gaagagatca ctgaagagca atattacatg atgcggagat
     1741 atgaaagctc agcctcacat cccccacctc gccctccgaa acacccctca gtagaggaaa
     1801 caaagttaac cctgctaacc ttagcagaag aaaggacggt agacctgccc aagtctccca
     1861 agcgtcatca cgtagacata accaagaaac ttcacccaaa tcaagctggc ctggattcaa
     1921 aagtactgat tggtagtcag aatgatttgg tggatgaaga gaaagaaagg agcaaccgtg
     1981 gggccacagc aatagcagaa acattcaaaa atgaaaaaca tcaaaaataa caagatgtga
     2041 cagaagccac ttaggcagca aacataaatg ttgcagtgaa aaaagaagct agccttctag
     2101 ctgaaaaacg agtattcccc aatggactcc agaagaaact tgattcatcg ctgcaaagga
     2161 aagaacaacc ttaaaacttt taacagataa aacttacaga aacctatgat atagaattca
     2221 tatagtctat tctgttgtgt ctaaatctgt aggcattgtg ttgttgttct ttaggacgta
     2281 tttatttaac ttgcacattt tttcagattc ttatttctac taccaacaac taagtaattg
     2341 ggaaataatt ctgtatttca gtttctgagt aaaaccagtc tgaaatagga taaaagccac
     2401 caaatatttt cttttttttc cagaatttgt tttgccattt tttagtgcta tcatcattcc
     2461 taacaagact aacttacaga aaaataatta tatctgactg atttaaaatg ttcaggtttc
     2521 ttatccaaat cccttggaac tatggaaagg agtttgattt cacattcaca gtgtatttac
     2581 aaaatacgct gtgtcataaa tatgtttgaa ttccaacagc caaagccatt gagagtcata
     2641 ggagttttcc ataaccttct cttctatgac ccaacaacaa gctcatgact gaaatttcac
     2701 cagatttctg agacgatgtc ttaatattct atgtgctatg taccagataa ttctttagat
     2761 gaatgtttct taggattgta ggaaaattat ctagttaatc ataatatttg atggaaagaa
     2821 aaagacaata aaattgtaat ataataaatt tggctgacaa gaaaccaaag tgattcttaa
     2881 ttagtataca tcagaatgat gctcttatag ttgtaccatc tataaaaatt actttaaggg
     2941 ctctcacatt ttaataattt atcttattat gtattaagta tacaggaaca atattatttt
     3001 tcctttaaca aaatgaagag acaggctatc tggttaatgt tacataggaa tttaatagta
     3061 atgcttgaac ttcatccata gatcatactc tgtacaaaat ttgttagcta acatcctatc
     3121 tcataattat tttatgtttt gtggagaaat ttgttgattt tgtaccaaag tgtttctgaa
     3181 gacaataaat tgtgagtcaa ctttagaaca aaaaaaatta gagttttttc aatgtttata
     3241 ttctgattaa gcttacttta ccttacattt tttctaagta acaatgaatc ctgatttcta
     3301 gtgtcctaaa aattgcttag tgatttgatt gtggtaatat catttcttat ctacaatgtc
     3361 taaagtttta tgggacagtt tttcttttat ttattttgcc tgtttgtgca gataagaaca
     3421 gaaacttttc taagacccac atttggttat tgaaggccac agcgaatctt aacctaacag
     3481 ccttgacaaa ctgcaccata ggtgttttta gactcatata atttgttatt tttcaaacaa
     3541 tagtgaataa ttaatatttt tgtttggaat ttgagaacaa ttaaatttgt acttttagta
     3601 actaccattc tttgattaga aaattaagag aatgcatatc ttactttggt tgtaaattat
     3661 caagggcttt ctaatagaaa tcatatataa catttctaaa tataagtcct ttcacatact
     3721 gtgtttccag ttgtcttgat attgaaaagt gtaataaact tcatgctcac ctattggaga
     3781 tttgggaagg ttgaaaataa acttcctaat tttta
//
