LOCUS       NM_001010923            3837 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens thymocyte selection associated (THEMIS), transcript
            variant 2, mRNA.
ACCESSION   NM_001010923 XM_376535
VERSION     NM_001010923.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Sun,C., Shou,P., Du,H., Hirabayashi,K., Chen,Y., Herring,L.E.,
            Ahn,S., Xu,Y., Suzuki,K., Li,G., Tsahouridis,O., Su,L., Savoldo,B.
            and Dotti,G.
  TITLE     THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated Priming of
            Chimeric Antigen Receptor-Redirected T Cells
  JOURNAL   Cancer Cell 37 (2), 216-225 (2020)
   PUBMED   32004441
  REMARK    GeneRIF: THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated
            Priming of Chimeric Antigen Receptor-Redirected T Cells
REFERENCE   3  (bases 1 to 3837)
  AUTHORS   Choi,S., Warzecha,C., Zvezdova,E., Lee,J., Argenty,J., Lesourne,R.,
            Aravind,L. and Love,P.E.
  TITLE     THEMIS enhances TCR signaling and enables positive selection by
            selective inhibition of the phosphatase SHP-1
  JOURNAL   Nat Immunol 18 (4), 433-441 (2017)
   PUBMED   28250424
  REMARK    GeneRIF: we found that THEMIS directly regulated the catalytic
            activity of the tyrosine phosphatase SHP-1.
            Erratum:[Nat Immunol. 2017 May 18;18(6):705. doi:
            10.1038/ni0617-705c.. PMID: 28518157]
REFERENCE   4  (bases 1 to 3837)
  AUTHORS   Kinosada,H., Yasunaga,J.I., Shimura,K., Miyazato,P., Onishi,C.,
            Iyoda,T., Inaba,K. and Matsuoka,M.
  TITLE     HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the
            Suppressive Signaling of Co-inhibitory Receptors
  JOURNAL   PLoS Pathog 13 (1), e1006120 (2017)
   PUBMED   28046066
  REMARK    Erratum:[PLoS Pathog. 2017 Feb 23;13(2):e1006228. doi:
            10.1371/journal.ppat.1006228.. PMID: 28231270]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3837)
  AUTHORS   Davies,J.L., Thompson,S., Kaur-Sandhu,H., Sawcer,S., Coles,A.,
            Ban,M. and Jones,J.
  TITLE     Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated
            with a Genotype that Is Protective against Multiple Sclerosis
  JOURNAL   PLoS One 11 (7), e0158327 (2016)
   PUBMED   27438997
  REMARK    GeneRIF: we have demonstrated mutually exclusive use of exon 1 and
            2 amongst 16 novel THEMIS isoforms. We also show inverse
            correlation between THEMIS expression in human CD4+ T-cells and
            dosage of the multiple sclerosis risk allele at rs13204742, driven
            by reduced expression of exon 1- containing isoforms
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3837)
  AUTHORS   Kakugawa,K., Yasuda,T., Miura,I., Kobayashi,A., Fukiage,H.,
            Satoh,R., Matsuda,M., Koseki,H., Wakana,S., Kawamoto,H. and
            Yoshida,H.
  TITLE     A novel gene essential for the development of single positive
            thymocytes
  JOURNAL   Mol Cell Biol 29 (18), 5128-5135 (2009)
   PUBMED   19620281
REFERENCE   7  (bases 1 to 3837)
  AUTHORS   Allen,P.M.
  TITLE     Themis imposes new law and order on positive selection
  JOURNAL   Nat Immunol 10 (8), 805-806 (2009)
   PUBMED   19621038
REFERENCE   8  (bases 1 to 3837)
  AUTHORS   Fu,G., Vallee,S., Rybakin,V., McGuire,M.V., Ampudia,J.,
            Brockmeyer,C., Salek,M., Fallen,P.R., Hoerter,J.A., Munshi,A.,
            Huang,Y.H., Hu,J., Fox,H.S., Sauer,K., Acuto,O. and Gascoigne,N.R.
  TITLE     Themis controls thymocyte selection through regulation of T cell
            antigen receptor-mediated signaling
  JOURNAL   Nat Immunol 10 (8), 848-856 (2009)
   PUBMED   19597499
  REMARK    GeneRIF: Functional analysis of the mouse gene by knockout, and
            comparison of the mouse and human proteins.
REFERENCE   9  (bases 1 to 3837)
  AUTHORS   Lesourne,R., Uehara,S., Lee,J., Song,K.D., Li,L., Pinkhasov,J.,
            Zhang,Y., Weng,N.P., Wildt,K.F., Wang,L., Bosselut,R. and Love,P.E.
  TITLE     Themis, a T cell-specific protein important for late thymocyte
            development
  JOURNAL   Nat Immunol 10 (8), 840-847 (2009)
   PUBMED   19597498
  REMARK    GeneRIF: Functional characterization of the homologous mouse gene.
            Erratum:[Nat Immunol. 2010 Jan;11(1):97]
REFERENCE   10 (bases 1 to 3837)
  AUTHORS   Johnson,A.L., Aravind,L., Shulzhenko,N., Morgun,A., Choi,S.Y.,
            Crockford,T.L., Lambe,T., Domaschenz,H., Kucharska,E.M., Zheng,L.,
            Vinuesa,C.G., Lenardo,M.J., Goodnow,C.C., Cornall,R.J. and
            Schwartz,R.H.
  TITLE     Themis is a member of a new metazoan gene family and is required
            for the completion of thymocyte positive selection
  JOURNAL   Nat Immunol 10 (8), 831-839 (2009)
   PUBMED   19597497
  REMARK    Erratum:[Nat Immunol. 2010 Jan;11(1):97]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035470.10, AL365224.8 and
            AL356432.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 23, 2021 this sequence version replaced NM_001010923.2.
            
            Summary: This gene encodes a protein that plays a regulatory role
            in both positive and negative T-cell selection during late
            thymocyte development. The protein functions through T-cell antigen
            receptor signaling, and is necessary for proper lineage commitment
            and maturation of T-cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the central coding region, compared to variant 1, resulting
            in an isoform (2) that is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC130516.1, AK292046.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368248.5/ ENSP00000357231.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL035470.10        93665-93881
            218-376             AL365224.8         32192-32350         c
            377-835             AL365224.8         6638-7096           c
            836-1884            AL356432.17        160081-161129       c
            1885-2020           AL356432.17        66886-67021         c
            2021-3837           AL356432.17        55398-57214         c
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.33"
     gene            1..3837
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="thymocyte selection associated"
                     /db_xref="GeneID:387357"
                     /db_xref="HGNC:HGNC:21569"
                     /db_xref="MIM:613607"
     exon            1..217
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..2052
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="isoform 2 is encoded by transcript variant 2;
                     thymocyte selection pathway associated; GRB2-associated
                     protein; signaling phosphoprotein specific for T cells;
                     thymocyte-expressed molecule involved in selection;
                     protein THEMIS"
                     /codon_start=1
                     /product="protein THEMIS isoform 2"
                     /protein_id="NP_001010923.1"
                     /db_xref="CCDS:CCDS34534.1"
                     /db_xref="GeneID:387357"
                     /db_xref="HGNC:HGNC:21569"
                     /db_xref="MIM:613607"
                     /translation="MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFS
                     TGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEIT
                     RTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQT
                     HSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKD
                     FYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEM
                     TSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLI
                     PTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVH
                     QSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELC
                     KQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGR
                     LNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEET
                     KLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSN
                     RGATAIAETFKNEKHQK"
     exon            218..376
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            377..835
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            836..1884
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2020
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..3837
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3818..3823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="hexamer: AATAAA"
     polyA_site      3837
                     /gene="THEMIS"
                     /gene_synonym="C6orf190; C6orf207; GASP; SPOT; THEMIS1;
                     TSEPA"
                     /note="major polyA site"
ORIGIN      
        1 gtagtgtgac attcctgcct gcctgaggaa gaaatggtga gcaggggctg caattgcaga
       61 caagtgtcac ccagaagcca caagtttctg tgagcaccag gtctacaaac tacccaaggc
      121 atagcaatgg cattatcact ggaagaattc gtccactccc ttgacctcag gaccctaccc
      181 agggttctag aaatccaggc aggcatctat cttgaaggct ctatttatga aatgtttgga
      241 aatgaatgct gtttttcaac aggagaagtg attaaaatta ctggtctcaa agttaagaag
      301 atcatagctg aaatttgtga gcagattgaa ggttgtgagt ctctacagcc atttgaactg
      361 cctatgaatt ttccaggtct ttttaagatt gtggctgata aaactccata ccttactatg
      421 gaagaaatca caaggaccat tcatattgga ccaagtagac tagggcatcc ttgcttctat
      481 catcagaagg atataaaact agagaacctc atcataaagc agggtgagca aatcatgctc
      541 aactcagttg aagagattga tggagaaata atggtgagct gtgcagtagc aaggaatcat
      601 caaactcact catttaattt gcctttgtca caagaaggag aattctacga gtgtgaagat
      661 gaacgtattt acactctaaa ggagattgtt gaatggaaga ttcctaagaa cagaacaaga
      721 actgtaaacc ttacagattt ttcaaataag tgggactcaa cgaatccatt tcctaaagac
      781 ttttatggta ccctgattct caagcctgtt tatgaaattc aaggtgtgat gaaatttcga
      841 aaagatataa tccgcatcct ccccagtcta gatgtcgaag tcaaagacat cactgattct
      901 tacgatgcta actggtttct tcagctgtta tcaacagaag atctttttga aatgactagt
      961 aaagagttcc ccatagtgac tgaagtcata gaagcacctg aaggaaacca cctgccccaa
     1021 agcattttac agcctgggaa aaccattgtg atccacaaaa agtaccaggc atcaagaatc
     1081 ttagcttcag aaattagaag caattttcct aaaagacact tcttgatccc cactagctat
     1141 aaaggcaagt tcaagcggcg accgagggag ttcccaacgg cctatgacct agagatcgct
     1201 aagagtgaaa aggagcctct tcacgtggtg gccaccaaag cgtttcattc ccctcatgac
     1261 aagctgtcat ccgtatctgt tggggaccag tttctggtgc atcagtcaga gacgactgaa
     1321 gtcctctgtg agggaataaa aaaagtggtg aatgttctgg cctgtgaaaa aatcctcaaa
     1381 aagtcctatg aggctgcgct gctccctttg tacatggaag gaggttttgt agaggtgatt
     1441 catgataaga aacagtaccc gatttctgag ctctgtaaac agttccgttt gcccttcaat
     1501 gtgaaggtgt ctgtcaggga tctttccatt gaagaggacg tgttggctgc cacaccagga
     1561 ctgcagttgg aggaggacat tacagactct tacctactca taagtgactt tgccaacccc
     1621 acggagtgct gggaaattcc tgtgggccgc ttgaatatga ctgttcagtt agttagtaat
     1681 ttctctaggg atgcagaacc atttctagtc aggactctgg tagaagagat cactgaagag
     1741 caatattaca tgatgcggag atatgaaagc tcagcctcac atcccccacc tcgccctccg
     1801 aaacacccct cagtagagga aacaaagtta accctgctaa ccttagcaga agaaaggacg
     1861 gtagacctgc ccaagtctcc caagcgtcat cacgtagaca taaccaagaa acttcaccca
     1921 aatcaagctg gcctggattc aaaagtactg attggtagtc agaatgattt ggtggatgaa
     1981 gagaaagaaa ggagcaaccg tggggccaca gcaatagcag aaacattcaa aaatgaaaaa
     2041 catcaaaaat aacaagatgt gacagaagcc acttaggcag caaacataaa tgttgcagtg
     2101 aaaaaagaag ctagccttct agctgaaaaa cgagtattcc ccaatggact ccagaagaaa
     2161 cttgattcat cgctgcaaag gaaagaacaa ccttaaaact tttaacagat aaaacttaca
     2221 gaaacctatg atatagaatt catatagtct attctgttgt gtctaaatct gtaggcattg
     2281 tgttgttgtt ctttaggacg tatttattta acttgcacat tttttcagat tcttatttct
     2341 actaccaaca actaagtaat tgggaaataa ttctgtattt cagtttctga gtaaaaccag
     2401 tctgaaatag gataaaagcc accaaatatt ttcttttttt tccagaattt gttttgccat
     2461 tttttagtgc tatcatcatt cctaacaaga ctaacttaca gaaaaataat tatatctgac
     2521 tgatttaaaa tgttcaggtt tcttatccaa atcccttgga actatggaaa ggagtttgat
     2581 ttcacattca cagtgtattt acaaaatacg ctgtgtcata aatatgtttg aattccaaca
     2641 gccaaagcca ttgagagtca taggagtttt ccataacctt ctcttctatg acccaacaac
     2701 aagctcatga ctgaaatttc accagatttc tgagacgatg tcttaatatt ctatgtgcta
     2761 tgtaccagat aattctttag atgaatgttt cttaggattg taggaaaatt atctagttaa
     2821 tcataatatt tgatggaaag aaaaagacaa taaaattgta atataataaa tttggctgac
     2881 aagaaaccaa agtgattctt aattagtata catcagaatg atgctcttat agttgtacca
     2941 tctataaaaa ttactttaag ggctctcaca ttttaataat ttatcttatt atgtattaag
     3001 tatacaggaa caatattatt tttcctttaa caaaatgaag agacaggcta tctggttaat
     3061 gttacatagg aatttaatag taatgcttga acttcatcca tagatcatac tctgtacaaa
     3121 atttgttagc taacatccta tctcataatt attttatgtt ttgtggagaa atttgttgat
     3181 tttgtaccaa agtgtttctg aagacaataa attgtgagtc aactttagaa caaaaaaaat
     3241 tagagttttt tcaatgttta tattctgatt aagcttactt taccttacat tttttctaag
     3301 taacaatgaa tcctgatttc tagtgtccta aaaattgctt agtgatttga ttgtggtaat
     3361 atcatttctt atctacaatg tctaaagttt tatgggacag tttttctttt atttattttg
     3421 cctgtttgtg cagataagaa cagaaacttt tctaagaccc acatttggtt attgaaggcc
     3481 acagcgaatc ttaacctaac agccttgaca aactgcacca taggtgtttt tagactcata
     3541 taatttgtta tttttcaaac aatagtgaat aattaatatt tttgtttgga atttgagaac
     3601 aattaaattt gtacttttag taactaccat tctttgatta gaaaattaag agaatgcata
     3661 tcttactttg gttgtaaatt atcaagggct ttctaataga aatcatatat aacatttcta
     3721 aatataagtc ctttcacata ctgtgtttcc agttgtcttg atattgaaaa gtgtaataaa
     3781 cttcatgctc acctattgga gatttgggaa ggttgaaaat aaacttccta attttta
//
