LOCUS       NM_003248               3222 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens thrombospondin 4 (THBS4), transcript variant 1, mRNA.
ACCESSION   NM_003248
VERSION     NM_003248.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Maemets-Allas,K., Klaas,M., Cardenas-Leon,C.G., Arak,T., Kankuri,E.
            and Jaks,V.
  TITLE     Stimulation with THBS4 activates pathways that regulate
            proliferation, migration and inflammation in primary human
            keratinocytes
  JOURNAL   Biochem Biophys Res Commun 642, 97-106 (2023)
   PUBMED   36566568
  REMARK    GeneRIF: Stimulation with THBS4 activates pathways that regulate
            proliferation, migration and inflammation in primary human
            keratinocytes.
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Andres Sastre,E., Maly,K., Zhu,M., Witte-Bouma,J., Trompet,D.,
            Bohm,A.M., Brachvogel,B., van Nieuwenhoven,C.A., Maes,C., van
            Osch,G.J.V.M., Zaucke,F. and Farrell,E.
  TITLE     Spatiotemporal distribution of thrombospondin-4 and -5 in cartilage
            during endochondral bone formation and repair
  JOURNAL   Bone 150, 115999 (2021)
   PUBMED   33971315
  REMARK    GeneRIF: Spatiotemporal distribution of thrombospondin-4 and -5 in
            cartilage during endochondral bone formation and repair.
REFERENCE   3  (bases 1 to 3222)
  AUTHORS   He,L., Wang,W., Shi,H., Jiang,C., Yao,H., Zhang,Y., Qian,W. and
            Lin,R.
  TITLE     THBS4/integrin alpha2 axis mediates BM-MSCs to promote angiogenesis
            in gastric cancer associated with chronic Helicobacter pylori
            infection
  JOURNAL   Aging (Albany NY) 13 (15), 19375-19396 (2021)
   PUBMED   34390328
  REMARK    GeneRIF: THBS4/integrin alpha2 axis mediates BM-MSCs to promote
            angiogenesis in gastric cancer associated with chronic Helicobacter
            pylori infection.
REFERENCE   4  (bases 1 to 3222)
  AUTHORS   Harada,J., Miyata,Y., Araki,K., Matsuda,T., Nagashima,Y., Mukae,Y.,
            Mitsunari,K., Matsuo,T., Ohba,K., Mochizuki,Y. and Sakai,H.
  TITLE     Pathological Significance and Prognostic Roles of Thrombospondin-3,
            4 and 5 in Bladder Cancer
  JOURNAL   In Vivo 35 (3), 1693-1701 (2021)
   PUBMED   33910854
  REMARK    GeneRIF: Pathological Significance and Prognostic Roles of
            Thrombospondin-3, 4 and 5 in Bladder Cancer.
REFERENCE   5  (bases 1 to 3222)
  AUTHORS   Newton,G., Weremowicz,S., Morton,C.C., Jenkins,N.A., Gilbert,D.J.,
            Copeland,N.G. and Lawler,J.
  TITLE     The thrombospondin-4 gene
  JOURNAL   Mamm Genome 10 (10), 1010-1016 (1999)
   PUBMED   10501972
REFERENCE   6  (bases 1 to 3222)
  AUTHORS   Arber,S. and Caroni,P.
  TITLE     Thrombospondin-4, an extracellular matrix protein expressed in the
            developing and adult nervous system promotes neurite outgrowth
  JOURNAL   J Cell Biol 131 (4), 1083-1094 (1995)
   PUBMED   7490284
  REMARK    GeneRIF: The functional analysis of the rat counterpart.
REFERENCE   7  (bases 1 to 3222)
  AUTHORS   Lawler,J., McHenry,K., Duquette,M. and Derick,L.
  TITLE     Characterization of human thrombospondin-4
  JOURNAL   J Biol Chem 270 (6), 2809-2814 (1995)
   PUBMED   7852353
REFERENCE   8  (bases 1 to 3222)
  AUTHORS   Adams,J.C. and Lawler,J.
  TITLE     Cell-type specific adhesive interactions of skeletal myoblasts with
            thrombospondin-1
  JOURNAL   Mol Biol Cell 5 (4), 423-437 (1994)
   PUBMED   7519904
REFERENCE   9  (bases 1 to 3222)
  AUTHORS   Lawler,J., Duquette,M., Whittaker,C.A., Adams,J.C., McHenry,K. and
            DeSimone,D.W.
  TITLE     Identification and characterization of thrombospondin-4, a new
            member of the thrombospondin gene family
  JOURNAL   J Cell Biol 120 (4), 1059-1067 (1993)
   PUBMED   8432726
REFERENCE   10 (bases 1 to 3222)
  AUTHORS   McLaren,K.M.
  TITLE     Immunohistochemical localisation of thrombospondin in human
            megakaryocytes and platelets
  JOURNAL   J Clin Pathol 36 (2), 197-199 (1983)
   PUBMED   6338048
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC050456.1 and AC112184.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003248.5.
            
            Summary: The protein encoded by this gene belongs to the
            thrombospondin protein family. Thrombospondin family members are
            adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix
            interactions. This protein forms a pentamer and can bind to heparin
            and calcium. It is involved in local signaling in the developing
            and adult nervous system, and it contributes to spinal
            sensitization and neuropathic pain states. This gene is activated
            during the stromal response to invasive breast cancer. It may also
            play a role in inflammatory responses in Alzheimer's disease.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z19585.1, SRR1803612.261873.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000350881.6/ ENSP00000339730.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BC050456.1         134-156
            24-24               AC112184.2         156344-156344
            25-3222             BC050456.1         158-3355
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.1"
     gene            1..3222
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /note="thrombospondin 4"
                     /db_xref="GeneID:7060"
                     /db_xref="HGNC:HGNC:11788"
                     /db_xref="MIM:600715"
     exon            1..278
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..3076
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /note="isoform a precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="thrombospondin-4 isoform a precursor"
                     /protein_id="NP_003239.2"
                     /db_xref="CCDS:CCDS4049.1"
                     /db_xref="GeneID:7060"
                     /db_xref="HGNC:HGNC:11788"
                     /db_xref="MIM:600715"
                     /translation="MLAPRGAAVLLLHLVLQRWLAAGAQATPQVFDLLPSSSQRLNPG
                     ALLPVLTDPALNDLYVISTFKLQTKSSATIFGLYSSTDNSKYFEFTVMGRLNKAILRY
                     LKNDGKVHLVVFNNLQLADGRRHRILLRLSNLQRGAGSLELYLDCIQVDSVHNLPRAF
                     AGPSQKPETIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEPLAATGTGD
                     FNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLKFQSPTPSTVVP
                     PAPPAPPTRPPRRCDSNPCFRGVQCTDSRDGFQCGPCPEGYTGNGITCIDVDECKYHP
                     CYPGVHCINLSPGFRCDACPVGFTGPMVQGVGISFAKSNKQVCTDIDECRNGACVPNS
                     ICVNTLGSYRCGPCKPGYTGDQIRGCKAERNCRNPELNPCSVNAQCIEERQGDVTCVC
                     GVGWAGDGYICGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACD
                     EDADGDGILNEQDNCVLIHNVDQRNSDKDIFGDACDNCLSVLNNDQKDTDGDGRGDAC
                     DDDMDGDGIKNILDNCPKFPNRDQRDKDGDGVGDACDSCPDVSNPNQSDVDNDLVGDS
                     CDTNQDSDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDN
                     CRLVPNPAQEDSNSDGVGDICESDFDQDQVIDRIDVCPENAEVTLTDFRAYQTVVLDP
                     EGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGF
                     IFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHT
                     GDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDT
                     TMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQNFDRFDN"
     exon            279..482
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            483..730
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            731..839
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            840..922
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            923..974
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1177
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1315
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1384
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1537
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1642
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1750
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1910
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2029
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2082
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2276
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2455
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2504
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2701
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2874
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            2875..3014
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3222
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3194..3199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /note="hexamer: AATAAA"
     polyA_site      3222
                     /gene="THBS4"
                     /gene_synonym="TSP-4; TSP4"
                     /note="major polyA site"
ORIGIN      
        1 agcagccagc tccccagcac cgcacggcgg ggacgcgagc gcgcccccga cggcagcccg
       61 gacgccgagc acgggtcacc tgcggcgccg gcccgggcgc cgaccgaggt tcaacgcacg
      121 gcccggggac ccccaggcgg ggccaacgcc gccgtcgccc ccggcctcgc ggggagcagg
      181 aagagccaac atgctggccc cgcgcggagc cgccgtcctc ctgctgcacc tggtcctgca
      241 gcggtggcta gcggcaggcg cccaggccac cccccaggtc tttgaccttc tcccatcttc
      301 cagtcagagg ctaaacccag gcgctctgct gccagtcctg acagaccccg ccctgaatga
      361 tctctatgtg atttccacct tcaagctgca gactaaaagt tcagccacca tcttcggtct
      421 ttactcttca actgacaaca gtaaatattt tgaatttact gtgatgggac gcttaaacaa
      481 agccatcctc cgttacctga agaacgatgg gaaggtgcat ttggtggttt tcaacaacct
      541 gcagctggca gacggaaggc ggcacaggat cctcctgagg ctgagcaatt tgcagcgagg
      601 ggccggctcc ctagagctct acctggactg catccaggtg gattccgttc acaatctccc
      661 cagggccttt gctggcccct cccagaaacc tgagaccatt gaattgagga ctttccagag
      721 gaagccacag gacttcttgg aagagctgaa gctggtggtg agaggctcac tgttccaggt
      781 ggccagcctg caagactgct tcctgcagca gagtgagcca ctggctgcca caggcacagg
      841 ggactttaac cggcagttct tgggtcaaat gacacaatta aaccaactcc tgggagaggt
      901 gaaggacctt ctgagacagc aggttaagga aacatcattt ttgcgaaaca ccatagctga
      961 atgccaggct tgcggtcctc tcaagtttca gtctccgacc ccaagcacgg tggtgccccc
     1021 ggctccccct gcaccgccaa cacgcccacc tcgtcggtgt gactccaacc catgtttccg
     1081 aggtgtccaa tgtaccgaca gtagagatgg cttccagtgt gggccctgcc ccgagggcta
     1141 cacaggaaac gggatcacct gtattgatgt tgatgagtgc aaataccatc cctgctaccc
     1201 gggcgtgcac tgcataaatt tgtctcctgg cttcagatgt gacgcctgcc cagtgggctt
     1261 cacagggccc atggtgcagg gtgttgggat cagttttgcc aagtcaaaca agcaggtctg
     1321 cactgacatt gatgagtgtc gaaatggagc gtgcgttccc aactcgatct gcgttaatac
     1381 tttgggatct taccgctgtg ggccttgtaa gccggggtat actggtgatc agataagggg
     1441 atgcaaagcg gaaagaaact gcagaaaccc agagctgaac ccttgcagtg tgaatgccca
     1501 gtgcattgaa gagaggcagg gggatgtgac atgtgtgtgt ggagtcggtt gggctggaga
     1561 tggctatatc tgtggaaagg atgtggacat cgacagttac cccgacgaag aactgccatg
     1621 ctctgccagg aactgtaaaa aggacaactg caaatatgtg ccaaattctg gccaagaaga
     1681 tgcagacaga gatggcattg gcgacgcttg tgacgaggat gctgacggag atgggatcct
     1741 gaatgagcag gataactgtg tcctgattca taatgtggac caaaggaaca gcgataaaga
     1801 tatctttggg gatgcctgtg ataactgcct gagtgtctta aataacgacc agaaagacac
     1861 cgatggggat ggaagaggag atgcctgtga tgatgacatg gatggagatg gaataaaaaa
     1921 cattctggac aactgcccaa aatttcccaa tcgtgaccaa cgggacaagg atggtgatgg
     1981 tgtgggggat gcctgtgaca gttgtcctga tgtcagcaac cctaaccagt ctgatgtgga
     2041 taatgatctg gttggggact cctgtgacac caatcaggac agtgatggag atgggcacca
     2101 ggacagcaca gacaactgcc ccaccgtcat taacagtgcc cagctggaca ccgataagga
     2161 tggaattggt gacgagtgtg atgatgatga tgacaatgat ggtatcccag acctggtgcc
     2221 ccctggacca gacaactgcc ggctggtccc caacccagcc caggaggata gcaacagcga
     2281 cggagtggga gacatctgtg agtctgactt tgaccaggac caggtcatcg atcggatcga
     2341 cgtctgccca gagaacgcag aggtcaccct gaccgacttc agggcttacc agaccgtggt
     2401 cctggatcct gaaggggatg cccagatcga tcccaactgg gtggtcctga accagggcat
     2461 ggagattgta cagaccatga acagtgatcc tggcctggca gtggggtaca cagcttttaa
     2521 tggagttgac ttcgaaggga ccttccatgt gaatacccag acagatgatg actatgcagg
     2581 ctttatcttt ggctaccaag atagctccag cttctacgtg gtcatgtgga agcagacgga
     2641 gcagacatat tggcaagcca ccccattccg agcagttgca gaacctggca ttcagctcaa
     2701 ggctgtgaag tctaagacag gtccagggga gcatctccgg aactccctgt ggcacacggg
     2761 ggacaccagt gaccaggtca ggctgctgtg gaaggactcc aggaatgtgg gctggaagga
     2821 caaggtgtcc taccgctggt tcctacagca caggccccag gtgggctaca tcagggtacg
     2881 attttatgaa ggctctgagt tggtggctga ctctggcgtc accatagaca ccacaatgcg
     2941 tggaggccga cttggcgttt tctgcttctc tcaagaaaac atcatctggt ccaacctcaa
     3001 gtatcgctgc aatgacacca tccctgagga cttccaagag tttcaaaccc agaatttcga
     3061 ccgcttcgat aattaaacca aggaagcaat ctgtaactgc ttttcggaac actaaaacca
     3121 tatatatttt aacttcaatt ttctttagct tttaccaacc caaatatatc aaaacgtttt
     3181 atgtgaatgt ggcaataaag gagaagagat catttttaaa aa
//
