LOCUS       NM_003247               5701 bp    mRNA    linear   PRI 28-JUN-2024
DEFINITION  Homo sapiens thrombospondin 2 (THBS2), transcript variant 1, mRNA.
ACCESSION   NM_003247
VERSION     NM_003247.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Hadar,N., Porgador,O., Cohen,I., Levi,H., Dolgin,V., Yogev,Y.,
            Sued-Hendrickson,S., Shelef,I., Didkovsky,E., Eskin-Schwartz,M. and
            Birk,O.S.
  TITLE     Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos syndrome
            with prominent vascular features in humans and mice
  JOURNAL   Eur J Hum Genet 32 (5), 550-557 (2024)
   PUBMED   38433265
  REMARK    GeneRIF: Heterozygous THBS2 pathogenic variant causes Ehlers-Danlos
            syndrome with prominent vascular features in humans and mice.
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Corbella,E., Fara,C., Covarelli,F., Porreca,V., Palmisano,B.,
            Mignogna,G., Corsi,A., Riminucci,M., Maras,B. and Mancone,C.
  TITLE     THBS1 and THBS2 Enhance the In Vitro Proliferation, Adhesion,
            Migration and Invasion of Intrahepatic Cholangiocarcinoma Cells
  JOURNAL   Int J Mol Sci 25 (3), 1782 (2024)
   PUBMED   38339060
  REMARK    GeneRIF: THBS1 and THBS2 Enhance the In Vitro Proliferation,
            Adhesion, Migration and Invasion of Intrahepatic Cholangiocarcinoma
            Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5701)
  AUTHORS   Kimura,T., Iwadare,T., Wakabayashi,S.I., Kuldeep,S., Nakajima,T.,
            Yamazaki,T., Aomura,D., Zafar,H., Iwaya,M., Joshita,S., Uehara,T.,
            Pydi,S.P., Tanaka,N. and Umemura,T.
  TITLE     Thrombospondin 2 is a key determinant of fibrogenesis in
            non-alcoholic fatty liver disease
  JOURNAL   Liver Int 44 (2), 483-496 (2024)
   PUBMED   38010940
  REMARK    GeneRIF: Thrombospondin 2 is a key determinant of fibrogenesis in
            non-alcoholic fatty liver disease.
REFERENCE   4  (bases 1 to 5701)
  AUTHORS   Zhou,X., Zhang,L., Lin,X., Chen,X., Liu,H., Yuan,X., Zhao,Q.,
            Wang,W., Lei,X., Jose,P.A., Deng,C. and Yang,J.
  TITLE     Thrombospondin 2 is a novel biomarker of essential hypertension and
            associated with nocturnal Na+ excretion and insulin resistance
  JOURNAL   Clin Exp Hypertens 45 (1), 2276029 (2023)
   PUBMED   37943619
  REMARK    GeneRIF: Thrombospondin 2 is a novel biomarker of essential
            hypertension and associated with nocturnal Na[+] excretion and
            insulin resistance.
REFERENCE   5  (bases 1 to 5701)
  AUTHORS   Wang,X., Xu,J., Hua,F., Wang,Y., Fang,G., Zhang,H. and Wu,X.
  TITLE     MiR-214-3p suppresses cervical cancer cell metastasis by
            downregulating THBS2
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 69 (9), 195-200 (2023)
   PUBMED   37807313
  REMARK    GeneRIF: MiR-214-3p suppresses cervical cancer cell metastasis by
            downregulating THBS2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5701)
  AUTHORS   LaBell,T.L. and Byers,P.H.
  TITLE     Sequence and characterization of the complete human thrombospondin
            2 cDNA: potential regulatory role for the 3' untranslated region
  JOURNAL   Genomics 17 (1), 225-229 (1993)
   PUBMED   8406456
REFERENCE   7  (bases 1 to 5701)
  AUTHORS   O'Rourke,K.M., Laherty,C.D. and Dixit,V.M.
  TITLE     Thrombospondin 1 and thrombospondin 2 are expressed as both homo-
            and heterotrimers
  JOURNAL   J Biol Chem 267 (35), 24921-24924 (1992)
   PUBMED   1459996
REFERENCE   8  (bases 1 to 5701)
  AUTHORS   LaBell,T.L., Milewicz,D.J., Disteche,C.M. and Byers,P.H.
  TITLE     Thrombospondin II: partial cDNA sequence, chromosome location, and
            expression of a second member of the thrombospondin gene family in
            humans
  JOURNAL   Genomics 12 (3), 421-429 (1992)
   PUBMED   1559694
REFERENCE   9  (bases 1 to 5701)
  AUTHORS   Breton-Gorius,J., Clezardin,P., Guichard,J., Debili,N., Malaval,L.,
            Vainchenker,W., Cramer,E.M. and Delmas,P.D.
  TITLE     Localization of platelet osteonectin at the internal face of the
            alpha-granule membranes in platelets and megakaryocytes
  JOURNAL   Blood 79 (4), 936-941 (1992)
   PUBMED   1737102
REFERENCE   10 (bases 1 to 5701)
  AUTHORS   McLaren,K.M.
  TITLE     Immunohistochemical localisation of thrombospondin in human
            megakaryocytes and platelets
  JOURNAL   J Clin Pathol 36 (2), 197-199 (1983)
   PUBMED   6338048
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX322234.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 20, 2020 this sequence version replaced NM_003247.4.
            
            Summary: The protein encoded by this gene belongs to the
            thrombospondin family. It is a disulfide-linked homotrimeric
            glycoprotein that mediates cell-to-cell and cell-to-matrix
            interactions. This protein has been shown to function as a potent
            inhibitor of tumor growth and angiogenesis. Studies of the mouse
            counterpart suggest that this protein may modulate the cell surface
            properties of mesenchymal cells and be involved in cell adhesion
            and migration. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC146676.1, SRR1803614.271131.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000617924.6/ ENSP00000482784.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               BX322234.7         64704-64826         c
            124-197             BX322234.7         61713-61786         c
            198-754             BX322234.7         59397-59953         c
            755-839             BX322234.7         57177-57261         c
            840-1036            BX322234.7         52742-52938         c
            1037-1177           BX322234.7         51432-51572         c
            1178-1274           BX322234.7         50579-50675         c
            1275-1445           BX322234.7         48605-48775         c
            1446-1622           BX322234.7         48150-48326         c
            1623-1796           BX322234.7         45714-45887         c
            1797-1924           BX322234.7         43870-43997         c
            1925-2077           BX322234.7         43644-43796         c
            2078-2296           BX322234.7         42960-43178         c
            2297-2404           BX322234.7         40552-40659         c
            2405-2564           BX322234.7         39102-39261         c
            2565-2683           BX322234.7         37160-37278         c
            2684-2918           BX322234.7         36125-36359         c
            2919-3146           BX322234.7         34228-34455         c
            3147-3418           BX322234.7         33177-33448         c
            3419-3516           BX322234.7         32410-32507         c
            3517-3656           BX322234.7         31178-31317         c
            3657-5701           BX322234.7         26765-28809         c
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..5701
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="thrombospondin 2"
                     /db_xref="GeneID:7058"
                     /db_xref="HGNC:HGNC:11786"
                     /db_xref="MIM:188061"
     exon            1..123
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            124..197
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..3664
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="thrombospondin-2 isoform 1 precursor"
                     /protein_id="NP_003238.2"
                     /db_xref="CCDS:CCDS34574.1"
                     /db_xref="GeneID:7058"
                     /db_xref="HGNC:HGNC:11786"
                     /db_xref="MIM:188061"
                     /translation="MVWRLVLLALWVWPSTQAGHQDKDTTFDLFSISNINRKTIGAKQ
                     FRGPDPGVPAYRFVRFDYIPPVNADDLSKITKIMRQKEGFFLTAQLKQDGKSRGTLLA
                     LEGPGLSQRQFEIVSNGPADTLDLTYWIDGTRHVVSLEDVGLADSQWKNVTVQVAGET
                     YSLHVGCDLIDSFALDEPFYEHLQAEKSRMYVAKGSARESHFRGLLQNVHLVFENSVE
                     DILSKKGCQQGQGAEINAISENTETLRLGPHVTTEYVGPSSERRPEVCERSCEELGNM
                     VQELSGLHVLVNQLSENLKRVSNDNQFLWELIGGPPKTRNMSACWQDGRFFAENETWV
                     VDSCTTCTCKKFKTICHQITCPPATCASPSFVEGECCPSCLHSVDGEEGWSPWAEWTQ
                     CSVTCGSGTQQRGRSCDVTSNTCLGPSIQTRACSLSKCDTRIRQDGGWSHWSPWSSCS
                     VTCGVGNITRIRLCNSPVPQMGGKNCKGSGRETKACQGAPCPIDGRWSPWSPWSACTV
                     TCAGGIRERTRVCNSPEPQYGGKACVGDVQERQMCNKRSCPVDGCLSNPCFPGAQCSS
                     FPDGSWSCGSCPVGFLGNGTHCEDLDECALVPDICFSTSKVPRCVNTQPGFHCLPCPP
                     RYRGNQPVGVGLEAAKTEKQVCEPENPCKDKTHNCHKHAECIYLGHFSDPMYKCECQT
                     GYAGDGLICGEDSDLDGWPNLNLVCATNATYHCIKDNCPHLPNSGQEDFDKDGIGDAC
                     DDDDDNDGVTDEKDNCQLLFNPRQADYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDA
                     CSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPLVHNPDQTDVDNDLVGD
                     QCDNNEDIDDDGHQNNQDNCPYISNANQADHDRDGQGDACDPDDDNDGVPDDRDNCRL
                     VFNPDQEDLDGDGRGDICKDDFDNDNIPDIDDVCPENNAISETDFRNFQMVPLDPKGT
                     TQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFG
                     YQSSSRFYVVMWKQVTQTYWEDQPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNT
                     PGQVRTLWHDPRNIGWKDYTAYRWHLTHRPKTGYIRVLVHEGKQVMADSGPIYDQTYA
                     GGRLGLFVFSQEMVYFSDLKYECRDI"
     exon            198..754
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            755..839
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            840..1036
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1177
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1274
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1445
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1622
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1796
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1924
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2077
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2296
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2404
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2564
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2683
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2918
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2919..3146
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3418
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3516
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3517..3656
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3657..5701
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4053..4058
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="hexamer: GATAAA"
     polyA_site      4074
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="major polyA site"
     regulatory      5392..5397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="hexamer: ATTAAA"
     polyA_site      5412
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="major polyA site"
     regulatory      5675..5680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
                     /note="hexamer: AATAAA"
     polyA_site      5676
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
     polyA_site      5701
                     /gene="THBS2"
                     /gene_synonym="EDSCLL3; TSP2"
ORIGIN      
        1 ggccacgtcc accggagcat cctgcactgc agggccggtc tctcgctcca gcagagcctg
       61 cgcctttctg actcggtccg gaacactgaa accagtcatc actgcatctt tttggcaaac
      121 caggagctca gctgcaggag gcaggatggt ctggaggctg gtcctgctgg ctctgtgggt
      181 gtggcccagc acgcaagctg gtcaccagga caaagacacg accttcgacc ttttcagtat
      241 cagcaacatc aaccgcaaga ccattggcgc caagcagttc cgcgggcccg accccggcgt
      301 gccggcttac cgcttcgtgc gctttgacta catcccaccg gtgaacgcag atgacctcag
      361 caagatcacc aagatcatgc ggcagaagga gggcttcttc ctcacggccc agctcaagca
      421 ggacggcaag tccaggggca cgctgttggc tctggagggc cccggtctct cccagaggca
      481 gttcgagatc gtctccaacg gccccgcgga cacgctggat ctcacctact ggattgacgg
      541 cacccggcat gtggtctccc tggaggacgt cggcctggct gactcgcagt ggaagaacgt
      601 caccgtgcag gtggctggcg agacctacag cttgcacgtg ggctgcgacc tcatagacag
      661 cttcgctctg gacgagccct tctacgagca cctgcaggcg gaaaagagcc ggatgtacgt
      721 ggccaaaggc tctgccagag agagtcactt caggggtttg cttcagaacg tccacctagt
      781 gtttgaaaac tctgtggaag atattctaag caagaagggt tgccagcaag gccagggagc
      841 tgagatcaac gccatcagtg agaacacaga gacgctgcgc ctgggtccgc atgtcaccac
      901 cgagtacgtg ggccccagct cggagaggag gcccgaggtg tgcgaacgct cgtgcgagga
      961 gctgggaaac atggtccagg agctctcggg gctccacgtc ctcgtgaacc agctcagcga
     1021 gaacctcaag agagtgtcga atgataacca gtttctctgg gagctcattg gtggccctcc
     1081 taagacaagg aacatgtcag cttgctggca ggatggccgg ttctttgcgg aaaatgaaac
     1141 gtgggtggtg gacagctgca ccacgtgtac ctgcaagaaa tttaaaacca tttgccacca
     1201 aatcacctgc ccgcctgcaa cctgcgccag tccatccttt gtggaaggcg aatgctgccc
     1261 ttcctgcctc cactcggtgg acggtgagga gggctggtct ccgtgggcag agtggaccca
     1321 gtgctccgtg acgtgtggct ctgggaccca gcagagaggc cggtcctgtg acgtcaccag
     1381 caacacctgc ttggggccct ccatccagac acgggcttgc agtctgagca agtgtgacac
     1441 ccgcatccgg caggacggcg gctggagcca ctggtcacct tggtcttcat gctctgtgac
     1501 ctgtggagtt ggcaatatca cacgcatccg tctctgcaac tccccagtgc cccagatggg
     1561 gggcaagaat tgcaaaggga gtggccggga gaccaaagcc tgccagggcg ccccatgccc
     1621 aatcgatggc cgctggagcc cctggtcccc gtggtcggcc tgcactgtca cctgtgccgg
     1681 tgggatccgg gagcgcaccc gggtctgcaa cagccctgag cctcagtacg gagggaaggc
     1741 ctgcgtgggg gatgtgcagg agcgtcagat gtgcaacaag aggagctgcc ccgtggatgg
     1801 ctgtttatcc aacccctgct tcccgggagc ccagtgcagc agcttccccg atgggtcctg
     1861 gtcatgcggc tcctgccctg tgggcttctt gggcaatggc acccactgtg aggacctgga
     1921 cgagtgtgcc ctggtccccg acatctgctt ctccaccagc aaggtgcctc gctgtgtcaa
     1981 cactcagcct ggcttccact gcctgccctg cccgccccga tacagaggga accagcccgt
     2041 cggggtcggc ctggaagcag ccaagacgga aaagcaagtg tgtgagcccg aaaacccatg
     2101 caaggacaag acacacaact gccacaagca cgcggagtgc atctacctgg gccacttcag
     2161 cgaccccatg tacaagtgcg agtgccagac aggctacgcg ggcgacgggc tcatctgcgg
     2221 ggaggactcg gacctggacg gctggcccaa cctcaatctg gtctgcgcca ccaacgccac
     2281 ctaccactgc atcaaggata actgccccca tctgccaaat tctgggcagg aagactttga
     2341 caaggacggg attggcgatg cctgtgatga tgacgatgac aatgacggtg tgaccgatga
     2401 gaaggacaac tgccagctcc tcttcaatcc ccgccaggct gactatgaca aggatgaggt
     2461 tggggaccgc tgtgacaact gcccttacgt gcacaaccct gcccagatcg acacagacaa
     2521 caatggagag ggtgacgcct gctccgtgga cattgatggg gacgatgtct tcaatgaacg
     2581 agacaattgt ccctacgtct acaacactga ccagagggac acggatggtg acggtgtggg
     2641 ggatcactgt gacaactgcc ccctggtgca caaccctgac cagaccgacg tggacaatga
     2701 ccttgttggg gaccagtgtg acaacaacga ggacatagat gacgacggcc accagaacaa
     2761 ccaggacaac tgcccctaca tctccaacgc caaccaggct gaccatgaca gagacggcca
     2821 gggcgacgcc tgtgaccctg atgatgacaa cgatggcgtc cccgatgaca gggacaactg
     2881 ccggcttgtg ttcaacccag accaggagga cttggacggt gatggacggg gtgatatttg
     2941 taaagatgat tttgacaatg acaacatccc agatattgat gatgtgtgtc ctgaaaacaa
     3001 tgccatcagt gagacagact tcaggaactt ccagatggtc cccttggatc ccaaagggac
     3061 cacccaaatt gatcccaact gggtcattcg ccatcaaggc aaggagctgg ttcagacagc
     3121 caactcggac cccggcatcg ctgtaggttt tgacgagttt gggtctgtgg acttcagtgg
     3181 cacattctac gtaaacactg accgggacga cgactatgcc ggcttcgtct ttggttacca
     3241 gtcaagcagc cgcttctatg tggtgatgtg gaagcaggtg acgcagacct actgggagga
     3301 ccagcccacg cgggcctatg gctactccgg cgtgtccctc aaggtggtga actccaccac
     3361 ggggacgggc gagcacctga ggaacgcgct gtggcacacg gggaacacgc cggggcaggt
     3421 gcgaacctta tggcacgacc ccaggaacat tggctggaag gactacacgg cctataggtg
     3481 gcacctgact cacaggccca agactggcta catcagagtc ttagtgcatg aaggaaaaca
     3541 ggtcatggca gactcaggac ctatctatga ccaaacctac gctggcgggc ggctgggtct
     3601 atttgtcttc tctcaagaaa tggtctattt ctcagacctc aagtacgaat gcagagatat
     3661 ttaaacaaga tttgctgcat ttccggcaat gccctgtgca tgccatggtc cctagacacc
     3721 tcagttcatt gtggtccttg tggcttctct ctctagcagc acctcctgtc ccttgacctt
     3781 aactctgatg gttcttcacc tcctgccagc aaccccaaac ccaagtgcct tcagaggata
     3841 aatatcaatg gaactcagag atgaacatct aacccactag aggaaaccag tttggtgata
     3901 tatgagactt tatgtggagt gaaaattggg catgccatta cattgctttt tcttgtttgt
     3961 ttaaaaagaa tgacgtttac atataaaatg taattactta ttgtatttat gtgtatatgg
     4021 agttgaaggg aatactgtgc ataagccatt atgataaatt aagcatgaaa aatattgctg
     4081 aactactttt ggtgcttaaa gttgtcacta ttcttgaatt agagttgctc tacaatgaca
     4141 cacaaatccc attaaataaa ttataaacaa gggtcaattc aaatttgaag taatgtttta
     4201 gtaaggagag attagaagac aacaggcata gcaaatgaca taagctaccg attaactaat
     4261 cggaacatgt aaaacagtta caaaaataaa cgaactctcc tcttgtccta caatgaaagc
     4321 cctcatgtgc agtagagatg cagtttcatc aaagaacaaa catccttgca aatgggtgtg
     4381 acgcggttcc agatgtggat ttggcaaaac ctcatttaag taaaaggtta gcagagcaaa
     4441 gtgcggtgct ttagctgctg cttgtgccgc tgtggcgtcg gggaggctcc tgcctgagct
     4501 tccttcccca gctttgctgc ctgagaggaa ccagagcaga cgcacaggcc ggaaaaggcg
     4561 catctaacgc gtatctaggc tttggtaact gcggacaagt tgcttttacc tgatttgatg
     4621 atacatttca ttaaggttcc agttataaat attttgttaa tatttattaa gtgactatag
     4681 aatgcaactc catttaccag taacttattt taaatatgcc tagtaacaca tatgtagtat
     4741 aatttctaga aacaaacatc taataagtat ataatcctgt gaaaatatga ggcttgataa
     4801 tattaggttg tcacgatgaa gcatgctaga agctgtaaca gaatacatag agaataatga
     4861 ggagtttatg atggaacctt aaatatataa tgttgccagc gattttagtt caatatttgt
     4921 tactgttatc tatctgctgt atatggaatt cttttaattc aaacgctgaa aagaatcagc
     4981 atttagtctt gccaggcaca cccaataatc agtcatgtgt aatatgcaca agtttgtttt
     5041 tgtttttgtt ttttttgttg gttggtttgt ttttttgctt taagttgcat gatctttctg
     5101 caggaaatag tcactcatcc cactccacat aaggggttta gtaagagaag tctgtctgtc
     5161 tgatgatgga tagggggcaa atctttttcc cctttctgtt aatagtcatc acatttctat
     5221 gccaaacagg aacaatccat aactttagtc ttaatgtaca cattgcattt tgataaaatt
     5281 aattttgttg tttcctttga ggttgatcgt tgtgttgttg ttttgctgca ctttttactt
     5341 ttttgcgtgt ggagctgtat tcccgagacc aacgaagcgt tgggatactt cattaaatgt
     5401 agcgactgtc aacagcgtgc aggttttctg tttctgtgtt gtggggtcaa ccgtacaatg
     5461 gtgtgggagt gacgatgatg tgagtattta gaatgtacca tattttttgt aaattattta
     5521 tgtttttcta aacaaattta tcgtataggt tgatgaaacg tcatgtgttt tgccaaagac
     5581 tgtaaatatt tatttatgtg ttcacatggt caaaatttca ccactgaaac cctgcactta
     5641 gctagaacct catttttaaa gattaacaac aggaaataaa ttgtaaaaaa ggttttctat
     5701 a
//
