LOCUS       NM_003246               7789 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens thrombospondin 1 (THBS1), mRNA.
ACCESSION   NM_003246
VERSION     NM_003246.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Hu,J., Qin,C., Xu,Y., Liu,X., Wei,X., Wu,J., Zhao,X., Chen,C. and
            Lin,Y.
  TITLE     Decreased thrombospondin-1 impairs endometrial stromal
            decidualization in unexplained recurrent spontaneous abortiondagger
  JOURNAL   Biol Reprod 111 (2), 448-462 (2024)
   PUBMED   38780057
  REMARK    GeneRIF: Decreased thrombospondin-1 impairs endometrial stromal
            decidualization in unexplained recurrent spontaneous
            abortiondagger.
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Fan,Y., Dong,S., Xia,Y., Yang,X., Lei,Q., Xu,F., Liang,D.,
            Liang,S., Zhang,M., Yang,F., Jing,Y., Li,L., Zhu,X., Bao,H.,
            Chen,Z. and Zeng,C.
  TITLE     Role of TSP-1 and its receptor ITGB3 in the renal
            tubulointerstitial injury of focal segmental glomerulosclerosis
  JOURNAL   J Biol Chem 300 (8), 107516 (2024)
   PUBMED   38960036
  REMARK    GeneRIF: Role of TSP-1 and its receptor ITGB3 in the renal
            tubulointerstitial injury of focal segmental glomerulosclerosis.
REFERENCE   3  (bases 1 to 7789)
  AUTHORS   Zhou,Y., Liu,S., Zheng,Y., Wang,Y. and Zhou,Y.
  TITLE     The role of THBS1 and PDGFD in the immune microenvironment of
            Helicobacter pylori-associated gastric cancer
  JOURNAL   Arab J Gastroenterol 25 (2), 194-204 (2024)
   PUBMED   38705811
  REMARK    GeneRIF: The role of THBS1 and PDGFD in the immune microenvironment
            of Helicobacter pylori-associated gastric cancer.
REFERENCE   4  (bases 1 to 7789)
  AUTHORS   Guo,Y., Huang,C., Qiu,L., Fu,J., Xu,C. and Yang,F.
  TITLE     CircTHBS1 promotes trophoblast cell migration and invasion and
            inhibits trophoblast apoptosis by regulating miR-136-3p/IGF2R axis
  JOURNAL   FASEB J 38 (7), e23598 (2024)
   PUBMED   38581244
  REMARK    GeneRIF: CircTHBS1 promotes trophoblast cell migration and invasion
            and inhibits trophoblast apoptosis by regulating miR-136-3p/IGF2R
            axis.
REFERENCE   5  (bases 1 to 7789)
  AUTHORS   Ma,X., Xu,L., Gong,S., Wu,N., Guo,J., Feng,X., Zhao,M., Qiu,S.,
            Sun,M., Zhang,C., Zhang,X., Ren,Z. and Zhang,P.
  TITLE     hsa_circ_0007919 promotes pancreatic cancer metastasis by
            modulating Sp1-mediated THBS1 transcription
  JOURNAL   FASEB J 38 (7), e23591 (2024)
   PUBMED   38572579
  REMARK    GeneRIF: hsa_circ_0007919 promotes pancreatic cancer metastasis by
            modulating Sp1-mediated THBS1 transcription.
REFERENCE   6  (bases 1 to 7789)
  AUTHORS   Burglin,T.R. and Barnes,T.M.
  TITLE     Introns in sequence tags
  JOURNAL   Nature 357 (6377), 367-368 (1992)
   PUBMED   1350660
REFERENCE   7  (bases 1 to 7789)
  AUTHORS   Murphy-Ullrich,J.E., Schultz-Cherry,S. and Hook,M.
  TITLE     Transforming growth factor-beta complexes with thrombospondin
  JOURNAL   Mol Biol Cell 3 (2), 181-188 (1992)
   PUBMED   1550960
REFERENCE   8  (bases 1 to 7789)
  AUTHORS   Tuszynski,G.P. and Kowalska,M.A.
  TITLE     Thrombospondin-induced adhesion of human platelets
  JOURNAL   J Clin Invest 87 (4), 1387-1394 (1991)
   PUBMED   2010551
REFERENCE   9  (bases 1 to 7789)
  AUTHORS   Dixit,V.M., Hennessy,S.W., Grant,G.A., Rotwein,P. and Frazier,W.A.
  TITLE     Characterization of a cDNA encoding the heparin and collagen
            binding domains of human thrombospondin
  JOURNAL   Proc Natl Acad Sci U S A 83 (15), 5449-5453 (1986)
   PUBMED   3461443
REFERENCE   10 (bases 1 to 7789)
  AUTHORS   Lawler,J.W., Slayter,H.S. and Coligan,J.E.
  TITLE     Isolation and characterization of a high molecular weight
            glycoprotein from human blood platelets
  JOURNAL   J Biol Chem 253 (23), 8609-8616 (1978)
   PUBMED   101549
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK304754.1, BC136470.1,
            X14787.1, BU676185.1, BM784452.1, BM512145.1, BG115371.1,
            BC015134.1, AI168683.1, AI147670.1 and AC037198.7.
            
            On May 17, 2019 this sequence version replaced NM_003246.3.
            
            Summary: The protein encoded by this gene is a subunit of a
            disulfide-linked homotrimeric protein. This protein is an adhesive
            glycoprotein that mediates cell-to-cell and cell-to-matrix
            interactions. This protein can bind to fibrinogen, fibronectin,
            laminin, type V collagen and integrins alpha-V/beta-1. This protein
            has been shown to play roles in platelet aggregation, angiogenesis,
            and tumorigenesis. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.50755.1,
                                           SRR1803615.23668.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260356.6/ ENSP00000260356.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               AK304754.1         2-189
            189-4982            BC136470.1         163-4956
            4983-4999           X14787.1           4918-4934
            5000-5582           BU676185.1         2-584
            5583-5602           X14787.1           5518-5537
            5603-5787           BM784452.1         62-246
            5788-5790           BM512145.1         194-196
            5791-5901           BG115371.1         2-112
            5902-6334           BC015134.1         4-436
            6335-6347           BC015134.1         438-450
            6348-7007           BC015134.1         452-1111
            7008-7239           AI168683.1         1-232               c
            7240-7241           AI147670.1         13-14               c
            7242-7789           AC037198.7         64264-64811
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..7789
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="thrombospondin 1"
                     /db_xref="GeneID:7057"
                     /db_xref="HGNC:HGNC:11785"
                     /db_xref="MIM:188060"
     exon            1..150
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            151..246
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..3692
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="thrombospondin-1p180; thrombospondin-p50;
                     glycoprotein G"
                     /codon_start=1
                     /product="thrombospondin-1 precursor"
                     /protein_id="NP_003237.2"
                     /db_xref="CCDS:CCDS32194.1"
                     /db_xref="GeneID:7057"
                     /db_xref="HGNC:HGNC:11785"
                     /db_xref="MIM:188060"
                     /translation="MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGS
                     GRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRG
                     TLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFV
                     QEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFV
                     FGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDE
                     LSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVD
                     SCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCS
                     TSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVT
                     CGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTC
                     GGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYP
                     DGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRF
                     TGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGY
                     AGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDD
                     DDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACA
                     ADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTC
                     DNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVP
                     NPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQ
                     NDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQ
                     SSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPG
                     QVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGG
                     RLGLFVFSQEMVFFSDLKYECRDP"
     exon            247..806
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            807..882
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1082
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1205
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1299
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1473
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1650
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1824
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1952
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2105
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2324
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2432
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2592
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2593..2711
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2712..2946
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2947..3174
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3175..3446
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3447..3544
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3545..3684
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3685..7789
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5762..5767
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      5782
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="major polyA site"
     regulatory      6067..6072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      6089
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
     regulatory      6988..6993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      7013
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
     regulatory      7126..7131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      7144
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
     regulatory      7213..7218
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      7241
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
     regulatory      7487..7492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
                     /note="hexamer: AATAAA"
     polyA_site      7512
                     /gene="THBS1"
                     /gene_synonym="THBS; THBS-1; TSP; TSP-1; TSP1"
ORIGIN      
        1 agccgctgcg cccgagctgg cctgcgagtt cagggctcct gtcgctctcc aggagcaacc
       61 tctactccgg acgcacaggc attccccgcg cccctccagc cctcgccgcc ctcgccaccg
      121 ctcccggccg ccgcgctccg gtacacacag gatccctgct gggcaccaac agctccacca
      181 tggggctggc ctggggacta ggcgtcctgt tcctgatgca tgtgtgtggc accaaccgca
      241 ttccagagtc tggcggagac aacagcgtgt ttgacatctt tgaactcacc ggggccgccc
      301 gcaaggggtc tgggcgccga ctggtgaagg gccccgaccc ttccagccca gctttccgca
      361 tcgaggatgc caacctgatc ccccctgtgc ctgatgacaa gttccaagac ctggtggatg
      421 ctgtgcgggc agaaaagggt ttcctccttc tggcatccct gaggcagatg aagaagaccc
      481 ggggcacgct gctggccctg gagcggaaag accactctgg ccaggtcttc agcgtggtgt
      541 ccaatggcaa ggcgggcacc ctggacctca gcctgaccgt ccaaggaaag cagcacgtgg
      601 tgtctgtgga agaagctctc ctggcaaccg gccagtggaa gagcatcacc ctgtttgtgc
      661 aggaagacag ggcccagctg tacatcgact gtgaaaagat ggagaatgct gagttggacg
      721 tccccatcca aagcgtcttc accagagacc tggccagcat cgccagactc cgcatcgcaa
      781 aggggggcgt caatgacaat ttccaggggg tgctgcagaa tgtgaggttt gtctttggaa
      841 ccacaccaga agacatcctc aggaacaaag gctgctccag ctctaccagt gtcctcctca
      901 cccttgacaa caacgtggtg aatggttcca gccctgccat ccgcactaac tacattggcc
      961 acaagacaaa ggacttgcaa gccatctgcg gcatctcctg tgatgagctg tccagcatgg
     1021 tcctggaact caggggcctg cgcaccattg tgaccacgct gcaggacagc atccgcaaag
     1081 tgactgaaga gaacaaagag ttggccaatg agctgaggcg gcctccccta tgctatcaca
     1141 acggagttca gtacagaaat aacgaggaat ggactgttga tagctgcact gagtgtcact
     1201 gtcagaactc agttaccatc tgcaaaaagg tgtcctgccc catcatgccc tgctccaatg
     1261 ccacagttcc tgatggagaa tgctgtcctc gctgttggcc cagcgactct gcggacgatg
     1321 gctggtctcc atggtccgag tggacctcct gttctacgag ctgtggcaat ggaattcagc
     1381 agcgcggccg ctcctgcgat agcctcaaca accgatgtga gggctcctcg gtccagacac
     1441 ggacctgcca cattcaggag tgtgacaaga gatttaaaca ggatggtggc tggagccact
     1501 ggtccccgtg gtcatcttgt tctgtgacat gtggtgatgg tgtgatcaca aggatccggc
     1561 tctgcaactc tcccagcccc cagatgaacg ggaaaccctg tgaaggcgaa gcgcgggaga
     1621 ccaaagcctg caagaaagac gcctgcccca tcaatggagg ctggggtcct tggtcaccat
     1681 gggacatctg ttctgtcacc tgtggaggag gggtacagaa acgtagtcgt ctctgcaaca
     1741 accccacacc ccagtttgga ggcaaggact gcgttggtga tgtaacagaa aaccagatct
     1801 gcaacaagca ggactgtcca attgatggat gcctgtccaa tccctgcttt gccggcgtga
     1861 agtgtactag ctaccctgat ggcagctgga aatgtggtgc ttgtccccct ggttacagtg
     1921 gaaatggcat ccagtgcaca gatgttgatg agtgcaaaga agtgcctgat gcctgcttca
     1981 accacaatgg agagcaccgg tgtgagaaca cggaccccgg ctacaactgc ctgccctgcc
     2041 ccccacgctt caccggctca cagcccttcg gccagggtgt cgaacatgcc acggccaaca
     2101 aacaggtgtg caagccccgt aacccctgca cggatgggac ccacgactgc aacaagaacg
     2161 ccaagtgcaa ctacctgggc cactatagcg accccatgta ccgctgcgag tgcaagcctg
     2221 gctacgctgg caatggcatc atctgcgggg aggacacaga cctggatggc tggcccaatg
     2281 agaacctggt gtgcgtggcc aatgcgactt accactgcaa aaaggataat tgccccaacc
     2341 ttcccaactc agggcaggaa gactatgaca aggatggaat tggtgatgcc tgtgatgatg
     2401 acgatgacaa tgataaaatt ccagatgaca gggacaactg tccattccat tacaacccag
     2461 ctcagtatga ctatgacaga gatgatgtgg gagaccgctg tgacaactgt ccctacaacc
     2521 acaacccaga tcaggcagac acagacaaca atggggaagg agacgcctgt gctgcagaca
     2581 ttgatggaga cggtatcctc aatgaacggg acaactgcca gtacgtctac aatgtggacc
     2641 agagagacac tgatatggat ggggttggag atcagtgtga caattgcccc ttggaacaca
     2701 atccggatca gctggactct gactcagacc gcattggaga tacctgtgac aacaatcagg
     2761 atattgatga agatggccac cagaacaatc tggacaactg tccctatgtg cccaatgcca
     2821 accaggctga ccatgacaaa gatggcaagg gagatgcctg tgaccacgat gatgacaacg
     2881 atggcattcc tgatgacaag gacaactgca gactcgtgcc caatcccgac cagaaggact
     2941 ctgacggcga tggtcgaggt gatgcctgca aagatgattt tgaccatgac agtgtgccag
     3001 acatcgatga catctgtcct gagaatgttg acatcagtga gaccgatttc cgccgattcc
     3061 agatgattcc tctggacccc aaagggacat cccaaaatga ccctaactgg gttgtacgcc
     3121 atcagggtaa agaactcgtc cagactgtca actgtgatcc tggactcgct gtaggttatg
     3181 atgagtttaa tgctgtggac ttcagtggca ccttcttcat caacaccgaa agggacgatg
     3241 actatgctgg atttgtcttt ggctaccagt ccagcagccg cttttatgtt gtgatgtgga
     3301 agcaagtcac ccagtcctac tgggacacca accccacgag ggctcaggga tactcgggcc
     3361 tttctgtgaa agttgtaaac tccaccacag ggcctggcga gcacctgcgg aacgccctgt
     3421 ggcacacagg aaacacccct ggccaggtgc gcaccctgtg gcatgaccct cgtcacatag
     3481 gctggaaaga tttcaccgcc tacagatggc gtctcagcca caggccaaag acgggtttca
     3541 ttagagtggt gatgtatgaa gggaagaaaa tcatggctga ctcaggaccc atctatgata
     3601 aaacctatgc tggtggtaga ctagggttgt ttgtcttctc tcaagaaatg gtgttcttct
     3661 ctgacctgaa atacgaatgt agagatccct aatcatcaaa ttgttgattg aaagactgat
     3721 cataaaccaa tgctggtatt gcaccttctg gaactatggg cttgagaaaa cccccaggat
     3781 cacttctcct tggcttcctt cttttctgtg cttgcatcag tgtggactcc tagaacgtgc
     3841 gacctgcctc aagaaaatgc agttttcaaa aacagactca gcattcagcc tccaatgaat
     3901 aagacatctt ccaagcatat aaacaattgc tttggtttcc ttttgaaaaa gcatctactt
     3961 gcttcagttg ggaaggtgcc cattccactc tgcctttgtc acagagcagg gtgctattgt
     4021 gaggccatct ctgagcagtg gactcaaaag cattttcagg catgtcagag aagggaggac
     4081 tcactagaat tagcaaacaa aaccaccctg acatcctcct tcaggaacac ggggagcaga
     4141 ggccaaagca ctaaggggag ggcgcatacc cgagacgatt gtatgaagaa aatatggagg
     4201 aactgttaca tgttcggtac taagtcattt tcaggggatt gaaagactat tgctggattt
     4261 catgatgctg actggcgtta gctgattaac ccatgtaaat aggcacttaa atagaagcag
     4321 gaaagggaga caaagactgg cttctggact tcctccctga tccccaccct tactcatcac
     4381 ctgcagtggc cagaattagg gaatcagaat caaaccagtg taaggcagtg ctggctgcca
     4441 ttgcctggtc acattgaaat tggtggcttc attctagatg tagcttgtgc agatgtagca
     4501 ggaaaatagg aaaacctacc atctcagtga gcaccagctg cctcccaaag gaggggcagc
     4561 cgtgcttata tttttatggt tacaatggca caaaattatt atcaacctaa ctaaaacatt
     4621 ccttttctct tttttcctga attatcatgg agttttctaa ttctctcttt tggaatgtag
     4681 atttttttta aatgctttac gatgtaaaat atttattttt tacttattct ggaagatctg
     4741 gctgaaggat tattcatgga acaggaagaa gcgtaaagac tatccatgtc atctttgttg
     4801 agagtcttcg tgactgtaag attgtaaata cagattattt attaactctg ttctgcctgg
     4861 aaatttaggc ttcatacgga aagtgtttga gagcaagtag ttgacattta tcagcaaatc
     4921 tcttgcaaga acagcacaag gaaaatcagt ctaataagct gctctgcccc ttgtgctcag
     4981 agtggatgtt atgggattct ttttttctct gttttatctt ttcaagtgga attagttggt
     5041 tatccatttg caaatgtttt aaattgcaaa gaaagccatg aggtcttcaa tactgtttta
     5101 ccccatccct tgtgcatatt tccagggaga aggaaagcat atacactttt ttctttcatt
     5161 tttccaaaag agaaaaaaat gacaaaaggt gaaacttaca tacaaatatt acctcatttg
     5221 ttgtgtgact gagtaaagaa tttttggatc aagcggaaag agtttaagtg tctaacaaac
     5281 ttaaagctac tgtagtacct aaaaagtcag tgttgtacat agcataaaaa ctctgcagag
     5341 aagtattccc aataaggaaa tagcattgaa atgttaaata caatttctga aagttatgtt
     5401 ttttttctat catctggtat accattgctt tatttttata aattattttc tcattgccat
     5461 tggaatagat atctcagatt gtgtagatat gctatttaaa taatttatca ggaaatactg
     5521 cctgtagagt tagtatttct atttttatat aatgtttgca cactgaattg aagaattgtt
     5581 ggttttttct tttttttgtt ttgttttttt tttttttttt ttttgctttt gacctcccat
     5641 ttttactatt tgccaatacc tttttctagg aatgtgcttt tttttgtaca catttttatc
     5701 cattttacat tctaaagcag tgtaagttgt atattactgt ttcttatgta caaggaacaa
     5761 caataaatca tatggaaatt tatatttata cttactgtat ccatgcttat ttgttctcta
     5821 ctggctttat gtcatgaagt atatgcgtaa ataccattca taaatcaata tagcatatac
     5881 aaaaataaat tacagtaagt catagcaaca ttcacagttt gtatgtgatt gagaaagact
     5941 gagttgctca ggcctaggct tagaatttgc tgcgtttgtg gaataaaaga acaaaatgat
     6001 acattagcct gccatatcaa aaacatataa aagagaaatt atccctaagt caagggcccc
     6061 cataagaata aaatttctta ttaaggtcat tagatgtcat tgaatccttt tcaaagtgca
     6121 gtatgaaaac aaagggaaaa acactgaagc acacgcaact ctcacagcga cattttctga
     6181 cccacgaatg atgccttggg tgggcaacac gattgcatgt tgtggagaca cttcggaagt
     6241 aaatgtggat gagggaggag ctgtccttgc aatgttgagc caagcattac agatacctcc
     6301 tcttgaagaa ggaataataa gtttaatcaa aaaagaagac taaaaaatgt aaaatttgga
     6361 aggaatccat aaatgcgtgt gtgtctaaat acaaattatc atgtgaagaa aaggcccaag
     6421 tgtaccaata agcagacctt gatttttgga tgggctaatt atgaatgtgg aatactgacc
     6481 agttaatttc cagttttaat gaaaacagat caaagaagaa attttatgag taggttaaag
     6541 gtctggcttt gaggtctatt aaacactaga aaggactggc tgggtgagat aaaatcttcc
     6601 ttgttgattt tcactctcat tctataaata ctcatctttc tgagtagcca tgatcacata
     6661 caaatgtaaa ttgccaaatc attttatagt accaaggtga agaagcagga actagaaagt
     6721 gttgataata gctgtggagt taggaaaact gatgtgaagg aaataattct ttgaaatggc
     6781 aaagaattaa ataccatcat tcattatcag aagagttcaa cgtttgaagt gctgggagat
     6841 aattctaatt cattcttgga tagtgaagca aaactgattg aaaataccaa gataagacag
     6901 aaaaagtgac tggaaagagg agcttttctt ccaggcatgt tccagtttca ccctaagact
     6961 gaccttcaaa taatcaggtt gtactgaaat aaaggacttg ttaaaaatta aaattatgtc
     7021 atcgagatga tagctttttt cctcctccaa cagtttattg tcatgtgttg tgggagagct
     7081 cgagtgaaga gcaataaact ccaggtctta taagaatgta catacaataa aggtggtgcc
     7141 agcagttttt ttttttctaa agagtcacat gtagaaaagc ctccagtatt aagctcctga
     7201 attcattcct taaataaatt ggctctctct ctcttctata atttcttttt ctttttattt
     7261 ttgagatgaa gtcttgctct gtcgcccagg ctggagtgca gtgacacaat ctcggctcac
     7321 tgcaacctct gcctccccgg ttcaagcaat tctccctcct gcctcagcct cccaagtagc
     7381 tgggactaca agcgcccgcc accaagcctg gctaattctg tatttttagt aaagacgggg
     7441 tttcaccttg ttccggacaa acactaagcc ctaaagggaa atccaaaata aaaacatcta
     7501 tttttaataa cactttctat ctaaatcagg gtgacttttt aaaaaaaatc cggaagcttt
     7561 ttgttgaatt acgttacaga cttagttacc agtccttgtt agagttacct tcagttgaca
     7621 tgctgtgaat ggtcccacct cttttatggc agaattcatt acttaaaata actctatttt
     7681 cttccccctt acctaaataa cagaaaggct cactatgtcc caaatatcat tggcagaagc
     7741 aaactataaa gtcataagcc ctttgcagtg caagtctaga aataatttt
//
