LOCUS       NM_024831               4477 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript
            variant 1, mRNA.
ACCESSION   NM_024831
VERSION     NM_024831.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Edwin,R.K., Acharya,L.P., Maity,S.K., Chakrabarti,P., Tantia,O.,
            Joshi,M.B., Satyamoorthy,K., Parsa,K.V.L. and Misra,P.
  TITLE     TGS1/PIMT knockdown reduces lipid accumulation in adipocytes,
            limits body weight gain and promotes insulin sensitivity in mice
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166896 (2024)
   PUBMED   37751782
  REMARK    GeneRIF: TGS1/PIMT knockdown reduces lipid accumulation in
            adipocytes, limits body weight gain and promotes insulin
            sensitivity in mice.
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Chen,L., Roake,C.M., Maccallini,P., Bavasso,F., Dehghannasiri,R.,
            Santonicola,P., Mendoza-Ferreira,N., Scatolini,L., Rizzuti,L.,
            Esposito,A., Gallotta,I., Francia,S., Cacchione,S., Galati,A.,
            Palumbo,V., Kobin,M.A., Tartaglia,G.G., Colantoni,A., Proietti,G.,
            Wu,Y., Hammerschmidt,M., De Pitta,C., Sales,G., Salzman,J.,
            Pellizzoni,L., Wirth,B., Di Schiavi,E., Gatti,M., Artandi,S.E. and
            Raffa,G.D.
  TITLE     TGS1 impacts snRNA 3'-end processing, ameliorates survival motor
            neuron-dependent neurological phenotypes in vivo and prevents
            neurodegeneration
  JOURNAL   Nucleic Acids Res 50 (21), 12400-12424 (2022)
   PUBMED   35947650
  REMARK    GeneRIF: TGS1 impacts snRNA 3'-end processing, ameliorates survival
            motor neuron-dependent neurological phenotypes in vivo and prevents
            neurodegeneration.
            Erratum:[Nucleic Acids Res. 2022 Nov 28;50(21):12596. doi:
            10.1093/nar/gkac789.. PMID: 36095137]
REFERENCE   3  (bases 1 to 4477)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 4477)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4477)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4477)
  AUTHORS   Wang,Y., Han,K.J., Pang,X.W., Vaughan,H.A., Qu,W., Dong,X.Y.,
            Peng,J.R., Zhao,H.T., Rui,J.A., Leng,X.S., Cebon,J., Burgess,A.W.
            and Chen,W.F.
  TITLE     Large scale identification of human hepatocellular
            carcinoma-associated antigens by autoantibodies
  JOURNAL   J Immunol 169 (2), 1102-1109 (2002)
   PUBMED   12097419
REFERENCE   7  (bases 1 to 4477)
  AUTHORS   Verheggen,C., Lafontaine,D.L., Samarsky,D., Mouaikel,J.,
            Blanchard,J.M., Bordonne,R. and Bertrand,E.
  TITLE     Mammalian and yeast U3 snoRNPs are matured in specific and related
            nuclear compartments
  JOURNAL   EMBO J 21 (11), 2736-2745 (2002)
   PUBMED   12032086
REFERENCE   8  (bases 1 to 4477)
  AUTHORS   Misra,P., Qi,C., Yu,S., Shah,S.H., Cao,W.Q., Rao,M.S.,
            Thimmapaya,B., Zhu,Y. and Reddy,J.K.
  TITLE     Interaction of PIMT with transcriptional coactivators CBP, p300,
            and PBP differential role in transcriptional regulation
  JOURNAL   J Biol Chem 277 (22), 20011-20019 (2002)
   PUBMED   11912212
  REMARK    GeneRIF: Interaction of PIMT with transcriptional coactivators CBP,
            p300, and PBP differential role in transcriptional regulation.
REFERENCE   9  (bases 1 to 4477)
  AUTHORS   Mouaikel,J., Verheggen,C., Bertrand,E., Tazi,J. and Bordonne,R.
  TITLE     Hypermethylation of the cap structure of both yeast snRNAs and
            snoRNAs requires a conserved methyltransferase that is localized to
            the nucleolus
  JOURNAL   Mol Cell 9 (4), 891-901 (2002)
   PUBMED   11983179
REFERENCE   10 (bases 1 to 4477)
  AUTHORS   Zhu,Y., Qi,C., Cao,W.Q., Yeldandi,A.V., Rao,M.S. and Reddy,J.K.
  TITLE     Cloning and characterization of PIMT, a protein with a
            methyltransferase domain, which interacts with and enhances nuclear
            receptor coactivator PRIP function
  JOURNAL   Proc Natl Acad Sci U S A 98 (18), 10380-10385 (2001)
   PUBMED   11517327
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM853546.1, DB082992.1, AF432215.1, AF286340.1 and AC018607.12.
            
            On May 17, 2019 this sequence version replaced NM_024831.7.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.246294.1,
                                           SRR1803615.245414.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260129.6/ ENSP00000260129.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BM853546.1         215-244
            31-131              DB082992.1         30-130
            132-219             BM853546.1         346-433
            220-561             DB082992.1         222-563
            562-2483            AF432215.1         524-2445
            2484-3478           AF286340.1         2788-3782
            3479-4477           AC018607.12        111145-112143       c
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.1"
     gene            1..4477
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /note="trimethylguanosine synthase 1"
                     /db_xref="GeneID:96764"
                     /db_xref="HGNC:HGNC:17843"
                     /db_xref="MIM:606461"
     exon            1..274
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..2735
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /EC_number="2.1.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CLL-associated antigen KW-2; cap-specific guanine-N2
                     methyltransferase; hepatocellular carcinoma-associated
                     antigen 137; nuclear receptor coactivator 6-interacting
                     protein; PRIP-interacting protein with methyltransferase
                     motif; cap-specific guanine-N(2) methyltransferase"
                     /codon_start=1
                     /product="trimethylguanosine synthase isoform 1"
                     /protein_id="NP_079107.6"
                     /db_xref="CCDS:CCDS34894.1"
                     /db_xref="GeneID:96764"
                     /db_xref="HGNC:HGNC:17843"
                     /db_xref="MIM:606461"
                     /translation="MCCEKWSRVAEMFLFIEEREDCKILCLCSRAFVEDRKLYNLGLK
                     GYYIRDSGNNSGDQATEEEEGGYSCGTAESHDSKGIGLDESELDSEAELMRSMGLPLQ
                     FGRITAHKDFEVSMNTRNKVKIKKKKHQKKYLDEIVQESWRKEYEEDDILASDDPSSI
                     EQYENTRTYELQSKKDTETENPPVENTLSPKLEITEKWEKYWNEYGGGLLWQSWQEKH
                     PGQALSSEPWNFPDTKEEWEQHYSQLYWYYLEQFQYWEAQGWTFDASQSCDTDTYTSK
                     TEADDKNDEKCMKVDLVSFPSSPIMVDNDSSGTSDKDHSEILDGISNIKLNSEEVTQS
                     QLDSCTSHDGHQQLSEVSSKRECPASGQSEPRNGGTNEESNSSGNTNTDPPAEDSQKS
                     SGANTSKDRPHASGTDGDESEEDPPEHKPSKLKRSHELDIDENPASDFDDSGSLLGFK
                     YGSGQKYGGIPNFSHRQVRYLEKNVKLKSKYLDMRRQIKMKNKHIFFTKESEKPFFKK
                     SKILSKVEKFLTWVNKPMDEEASQESSSHDNVHDASTSSDSEEQDMSVKKGDDLLETN
                     NPEPEKCQSVSSAGELETENYERDSLLATVPDEQDCVTQEVPDSRQAETEAEVKKKKN
                     KKKNKKVNGLPPEIAAVPELAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIA
                     GRVSQSFKCDVVVDAFCGVGGNTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADK
                     IEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKIT
                     NNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET"
     exon            275..339
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            340..512
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            513..1335
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1453
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1540
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1715
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..2022
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2172
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2316
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2533
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2612
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..4477
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3457..3462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /note="hexamer: ATTAAA"
     polyA_site      3478
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /note="major polyA site"
     regulatory      3822..3827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
                     /note="hexamer: AATAAA"
     polyA_site      3849
                     /gene="TGS1"
                     /gene_synonym="NCOA6IP; PIMT; PIPMT"
ORIGIN      
        1 acttccggcg gcagcgtccg ggctagttcc cggcgcgagc ggccgcgggc cagtttctat
       61 ctcctcatcc agggcttgcg ggcgaggcct gttttaagtc tccagtaacc gagcggaggc
      121 ccggcaggcg cgacccgggc tgcgtacgtc agagctgcct ccgaagtggt aaaatgtgct
      181 gcgagaagtg gagccgcgtg gcggaaatgt ttctcttcat tgaggagcgg gaggattgta
      241 agatactgtg cctttgctcc agggcatttg tggaggatcg aaaattgtac aatttgggat
      301 taaaaggcta ttacatcaga gacagtggca acaattcagg agaccaggcg acagaagaag
      361 aggaaggtgg ttattcctgt ggtactgcag aatcacatga cagcaaaggc ataggcctgg
      421 atgaaagtga acttgattct gaggctgaac tcatgagaag tatgggattg ccacttcaat
      481 ttggtaggat aactgcacat aaggattttg aggtatctat gaatactaga aataaagtta
      541 aaataaaaaa gaaaaaacat caaaagaaat acttagatga aattgtgcaa gaatcttgga
      601 gaaaagaata tgaagaagac gacattttgg cttcagatga tccatcttca attgaacagt
      661 atgagaacac cagaacatat gaacttcaaa gcaaaaaaga tactgagaca gaaaatcctc
      721 cagttgaaaa cacattatct ccaaagctag aaattacaga gaaatgggaa aagtattgga
      781 atgaatatgg aggaggacta ttgtggcaaa gttggcaaga aaaacatccg ggtcaagcac
      841 tatcttctga accttggaac tttcctgata caaaggaaga atgggagcaa cattatagtc
      901 aactttattg gtattatttg gaacaatttc agtattggga agctcagggt tggacttttg
      961 atgcctcgca aagctgtgat acagatactt acacatctaa aacagaagct gatgacaaga
     1021 acgatgaaaa atgcatgaaa gttgacttag tatcttttcc atcttcacct attatggttg
     1081 ataatgatag ctctggtaca agtgataagg atcatagtga aatacttgat ggaattagta
     1141 acataaaact gaattcagag gaagtaacac agagccaatt agattcctgt acaagtcatg
     1201 atggtcatca acagctaagt gaagttagta gcaaaagaga gtgccctgct tccggccaaa
     1261 gtgaaccacg taatggagga accaatgagg aaagcaactc atcggggaat acaaacacag
     1321 acccaccagc tgaggattca cagaagtctt caggagcaaa cacaagcaaa gacagaccac
     1381 atgccagtgg tactgatgga gatgaaagtg aggaagaccc acctgagcat aagccaagca
     1441 aactgaagag gagccatgaa ctggacattg atgaaaaccc agcttcagac tttgatgaca
     1501 gtggttccct tctaggattc aagtatggct caggacaaaa atatggtgga atcccaaatt
     1561 tcagtcatcg gcaggtcagg tatttagaga agaatgtgaa gcttaagtct aagtacctag
     1621 acatgcgcag acaaataaag atgaaaaaca aacacatctt ctttaccaaa gagtcagaaa
     1681 aaccattttt caagaaaagc aaaattctga gtaaggtaga aaaattcctc acatgggtta
     1741 ataaaccaat ggatgaagaa gcatcacagg aatcatcttc tcatgacaat gtgcacgacg
     1801 cttccacaag tagtgattca gaggaacaag acatgtctgt taaaaaaggt gatgacctac
     1861 tggagactaa taatccagaa cctgaaaagt gtcagagcgt atcttcagct ggtgaacttg
     1921 aaacagaaaa ctatgaaaga gacagcttgc tagcaactgt tccagatgag caggattgtg
     1981 ttactcaaga agtgccagac tcccgccagg cagaaactga agctgaagtg aaaaagaaga
     2041 agaacaagaa gaagaacaaa aaggtgaatg gtctgcctcc tgaaatagct gctgttcctg
     2101 agctggcaaa atactgggcc cagaggtaca ggctcttctc ccgttttgat gatgggatta
     2161 agttggacag agagggctgg ttttcagtta cacccgagaa gattgctgaa cacattgctg
     2221 gccgtgttag tcagtccttc aagtgtgacg ttgtagtaga cgcattctgt ggagttggag
     2281 gaaataccat tcagtttgcc ttaacaggaa tgagagtgat tgccattgat atcgatcctg
     2341 ttaagattgc ccttgctcgc aataatgcag aagtttatgg gatagcagat aagatagagt
     2401 tcatctgtgg agatttcttg ctgctggctt cttttttaaa ggctgatgtt gtgttcctca
     2461 gcccaccttg gggagggcca gactatgcca ctgcagagac ctttgacatt agaacaatga
     2521 tgtctcctga tggctttgaa attttcagac tttctaagaa gatcactaat aatattgttt
     2581 attttcttcc aagaaatgct gatattgacc aggtggcatc cttagctggg cctggagggc
     2641 aagtggaaat agaacagaac ttccttaaca acaaattgaa gacaatcact gcatattttg
     2701 gtgacctaat tcgaagacca gcctctgaaa cctaactatg cagcagtgcg aggacaaaag
     2761 atcatggagt ggtcaaaata ttcagatgag acatttggca tgtcttcctt tattcactga
     2821 tattttctac ccatggtctt atatcaccgt atgaaatgga aacttacagg acttaaatat
     2881 cagtgaaata ttttgagatc tttgaataat tcctttagag gaattataca aaattaatat
     2941 atatgagtcc tttgtaattt attttttttt gagacaggat ctcactttta ccgcccaggc
     3001 tggagtgcag tgccatgatc acagctcact gcaggttcag ccttctgagt tcaagcaatc
     3061 cttctgtctc agtctcctca gtagctgggg ctttaggtgg gcactgccac accgtactaa
     3121 tttttgtatt ttttgtagag acgaggtccc accatgttgc ccaggctggt gtcaaactcc
     3181 tgggctcagt cagtcccccc atctcaccct ccccaagtgc tggaattaca ggcgtgagct
     3241 actgtgccca gccttacgga catccttttg aattatcttt ttcactcata gaatatgaat
     3301 acatttattt agactttttc tagaactttc ctgttttcat gtctttgctt catctggaat
     3361 tggcttaaca cccttttata aagtttgtgt ttgtaaaatt tccattgtga catcaatacg
     3421 caatatattt tgtaatatag gagtttctat ttttttatta aaatggcaat gaaagcaaga
     3481 gggatatgtg ttgcttaatt attcatctaa aaagtttgtt cagtcatttt tacaagtagg
     3541 caaaaaaata gtgacataca acacttgtcc attgaaggtt agacctggga cttctatatt
     3601 ttaataatat gagcgtgtta aacattaaac agaacatgat actggctggg ataagattag
     3661 agaattgaaa caaatttggg gaatttccaa taaatactag aaaattagac tagaaaaata
     3721 gctgttatat aaataacttt gttggaatat gctaatttta gtgtcttgaa agtttacaat
     3781 ttatctgatt gctctgcagt acagtagcaa ctgagctgat caataaaggt gaaattactt
     3841 tttcattaat tcgcaatttc aaaatctgtc caaatgttct tggtttttaa actttatgta
     3901 gcattctttt tttttttttt agacagagtc ttgctctgtc gcccaggctg gagtgcaatg
     3961 gcgtgatctc agcttactgc aacctccacc tcctacctca gcctcccgag tagctgggac
     4021 tacaggcacc tgccaccaca tccagctaat tttttatatt tttagtagct atggggtttc
     4081 accgtgttag ccaggatggt ctcgatctcc tgaccttgtg atccaaccac ctcagcctcc
     4141 cgtagttctg ggattacagg cgtgagccac cgcgcctggc tttatgtagc attcttaaag
     4201 tcactaggga gatggcaggt gaagtaaagg cagatttcca aaatcactaa ttttttttag
     4261 ttttttgtca cttaaccttt cttgcatata cttctttcca cagctcattt tcttacttgt
     4321 atattaataa agctaactca tcttcctgaa tatacatgat tttacatgag aagagcagga
     4381 agcttaggct tggttagcta aaactaagat attgctgccc actaatcaca cagggataaa
     4441 actaatagac tgttaagatt gtcagtactc aatagga
//
