LOCUS       NM_004257               5680 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens transforming growth factor beta receptor associated
            protein 1 (TGFBRAP1), transcript variant 1, mRNA.
ACCESSION   NM_004257 NM_001426428
VERSION     NM_004257.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5680)
  AUTHORS   van der Beek,J., Jonker,C., van der Welle,R., Liv,N. and
            Klumperman,J.
  TITLE     CORVET, CHEVI and HOPS - multisubunit tethers of the endo-lysosomal
            system in health and disease
  JOURNAL   J Cell Sci 132 (10) (2019)
   PUBMED   31092635
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5680)
  AUTHORS   Yang,S., Chen,X., Yang,M., Zhao,X., Chen,Y., Zhao,H., Liu,C. and
            Shen,C.
  TITLE     The variant at TGFBRAP1 is significantly associated with type 2
            diabetes mellitus and affects diabetes-related miRNA expression
  JOURNAL   J Cell Mol Med 23 (1), 83-92 (2019)
   PUBMED   30461200
  REMARK    GeneRIF: Findings suggest that genetic polymorphisms of TGF-beta1
            receptor-associated protein 1 (TGFBRAP1) may contribute to the
            genetic susceptibility of type 2 diabetes mellitus (T2DM) by
            mediating diabetes-related miRNA expression.
REFERENCE   5  (bases 1 to 5680)
  AUTHORS   Jonker,C.T.H., Galmes,R., Veenendaal,T., Ten Brink,C., van der
            Welle,R.E.N., Liv,N., de Rooij,J., Peden,A.A., van der Sluijs,P.,
            Margadant,C. and Klumperman,J.
  TITLE     Vps3 and Vps8 control integrin trafficking from early to recycling
            endosomes and regulate integrin-dependent functions
  JOURNAL   Nat Commun 9 (1), 792 (2018)
   PUBMED   29476049
  REMARK    GeneRIF: Vps3 and Vps8 are required for recycling of beta1
            integrins.
            Erratum:[Nat Commun. 2021 Sep 29;12(1):5828. doi:
            10.1038/s41467-021-25201-6.. PMID: 34588435]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5680)
  AUTHORS   Le Clerc,S., Limou,S., Coulonges,C., Carpentier,W., Dina,C.,
            Taing,L., Delaneau,O., Labib,T., Sladek,R., Deveau,C.,
            Guillemain,H., Ratsimandresy,R., Montes,M., Spadoni,J.L.,
            Therwath,A., Schachter,F., Matsuda,F., Gut,I., Lelievre,J.D.,
            Levy,Y., Froguel,P., Delfraissy,J.F., Hercberg,S. and Zagury,J.F.
  CONSRTM   ANRS Genomic Group
  TITLE     Genomewide association study of a rapid progression cohort
            identifies new susceptibility alleles for AIDS (ANRS Genomewide
            Association Study 03)
  JOURNAL   J Infect Dis 200 (8), 1194-1201 (2009)
   PUBMED   19754311
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
REFERENCE   7  (bases 1 to 5680)
  AUTHORS   Hwang,Y., Chen,E.Y., Gu,Z.J., Chuang,W.L., Yu,M.L., Lai,M.Y.,
            Chao,Y.C., Lee,C.M., Wang,J.H., Dai,C.Y., Shian-Jy Bey,M.,
            Liao,Y.T., Chen,P.J. and Chen,D.S.
  TITLE     Genetic predisposition of responsiveness to therapy for chronic
            hepatitis C
  JOURNAL   Pharmacogenomics 7 (5), 697-709 (2006)
   PUBMED   16886895
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   8  (bases 1 to 5680)
  AUTHORS   Wurthner,J.U., Frank,D.B., Felici,A., Green,H.M., Cao,Z.,
            Schneider,M.D., McNally,J.G., Lechleider,R.J. and Roberts,A.B.
  TITLE     Transforming growth factor-beta receptor-associated protein 1 is a
            Smad4 chaperone
  JOURNAL   J Biol Chem 276 (22), 19495-19502 (2001)
   PUBMED   11278302
REFERENCE   9  (bases 1 to 5680)
  AUTHORS   Provost,P., Samuelsson,B. and Radmark,O.
  TITLE     Interaction of 5-lipoxygenase with cellular proteins
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 1881-1885 (1999)
   PUBMED   10051563
REFERENCE   10 (bases 1 to 5680)
  AUTHORS   Charng,M.J., Zhang,D., Kinnunen,P. and Schneider,M.D.
  TITLE     A novel protein distinguishes between quiescent and activated forms
            of the type I transforming growth factor beta receptor
  JOURNAL   J Biol Chem 273 (16), 9365-9368 (1998)
   PUBMED   9545258
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012360.7, BC020548.2 and
            BC019841.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Dec 3, 2024 this sequence version replaced
            NM_001426428.1, NM_004257.5.
            
            Summary: This gene encodes a protein that binds to transforming
            growth factor-beta (TGF-beta) receptors and plays a role in
            TGF-beta signaling. The encoded protein acts as a chaprone in
            signaling downstream of TGF-beta. It is involved in
            signal-dependent association with SMAD4. The protein is also a
            component of mammalian CORVET, a multisubunit tethering protein
            complex that is involved in fusion of early endosomes. [provided by
            RefSeq, Jun 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, 5, and 6 all encode the same isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020548.2, SRR11853561.16539.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393359.7/ ENSP00000377027.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC012360.7         41252-41314
            64-376              BC020548.2         1-313
            377-731             AC012360.7         62934-63288
            732-850             BC020548.2         669-787
            851-851             AC012360.7         72316-72316
            852-3022            BC020548.2         789-2959
            3023-4490           AC012360.7         103916-105383
            4491-5680           BC019841.1         631-1820
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.1-q12.2"
     gene            1..5680
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="transforming growth factor beta receptor associated
                     protein 1"
                     /db_xref="GeneID:9392"
                     /db_xref="HGNC:HGNC:16836"
                     /db_xref="MIM:606237"
     exon            1..111
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            112..816
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..2711
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     TGF-beta receptor-associated protein 1; VPS3 CORVET
                     complex subunit"
                     /codon_start=1
                     /product="transforming growth factor-beta
                     receptor-associated protein 1 isoform 1"
                     /protein_id="NP_004248.2"
                     /db_xref="CCDS:CCDS2067.1"
                     /db_xref="GeneID:9392"
                     /db_xref="HGNC:HGNC:16836"
                     /db_xref="MIM:606237"
                     /translation="MMSIKAFTLVSAVERELLMGDKERVNIECVECCGRDLYVGTNDC
                     FVYHFLLEERPVPAGPATFTATKQLQRHLGFKKPVNELRAASALNRLLVLCDNSISLV
                     NMLNLEPVPSGARIKGAATFALNENPVSGDPFCVEVCIISVKRRTIQMFLVYEDRVQI
                     VKEVSTAEQPLAVAVDGHFLCLALTTQYIIHNYSTGVSQDLFPYCSEERPPIVKRIGR
                     QEFLLAGPGGLGMFATVAGISQRAPVHWSENVIGAAVSFPYVIALDDEFITVHSMLDQ
                     QQKQTLPFKEGHILQDFEGRVIVATSKGVYILVPLPLEKQIQDLLASRRVEEALVLAK
                     GARRNIPKEKFQVMYRRILQQAGFIQFAQLQFLEAKELFRSGQLDVRELISLYPFLLP
                     TSSSFTRSHPPLHEYADLNQLTQGDQEKMAKCKRFLMSYLNEVRSTEVANGYKEDIDT
                     ALLKLYAEADHDSLLDLLVTENFCLLTDSAAWLEKHKKYFALGLLYHYNNQDAAAVQL
                     WVNIVNGDVQDSTRSDLYEYIVDFLTYCLDEELVWAYADWVLQKSEEVGVQVFTKRPL
                     DEQQKNSFNPDDIINCLKKYPKALVKYLEHLVIDKRLQKEEYHTHLAVLYLEEVLLQR
                     ASASGKGAEATETQAKLRRLLQKSDLYRVHFLLERLQGAGLPMESAILHGKLGEHEKA
                     LHILVHELQDFAAAEDYCLWCSEGRDPPHRQQLFHTLLAIYLHAGPTAHELAVAAVDL
                     LNRHATEFDAAQVLQMLPDTWSVQLLCPFLMGAMRDSIHARRTMQVALGLARSENLIY
                     TYDKMKLKGSSIQLSDKKLCQICQNPFCEPVFVRYPNGGLVHTHCAASRHTNPSSSSP
                     GTRT"
     exon            817..1011
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1166
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1249
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1591
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1649
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1793
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1940
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2100
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2534
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..5680
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4350..4355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="hexamer: AATAAA"
     regulatory      4361..4366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="hexamer: AAGAAA"
     polyA_site      4380
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="major polyA site"
     polyA_site      4383
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="major polyA site"
     regulatory      5660..5665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="hexamer: AATAAA"
     polyA_site      5680
                     /gene="TGFBRAP1"
                     /gene_synonym="TRAP-1; TRAP1; VPS3"
                     /note="major polyA site"
ORIGIN      
        1 gtgacgggaa gcggcggctg cggggtcggg ccagcccagg agccgcgggc cggagcgggg
       61 cggcggggcc ccaggccgcg gggcggcgcg ggacggcggg cgccggcgcc gcagatcagc
      121 cagtagacat gatgagcatc aaagccttta cgcttgtctc tgctgtggag cgggagctgc
      181 tgatgggcga caaggagcgc gtcaacatag agtgcgtgga gtgctgcggc agggacctct
      241 acgtgggcac caacgactgc ttcgtctacc acttcctgtt ggaggagagg ccagtgcctg
      301 ctgggccagc cacgttcact gccaccaaac agctgcagag acacttgggc ttcaagaagc
      361 ccgtgaacga gctgcgtgcg gcctcagcac tcaacaggct gctggtgctg tgtgacaact
      421 ccatcagcct ggtcaacatg ctgaacctcg agccagtgcc ttcgggggcc cgcatcaagg
      481 gggcagccac gtttgcactg aacgagaacc ctgtgagtgg ggaccccttc tgtgtagaag
      541 tttgcatcat ctctgtcaaa cgcagaacca tccagatgtt tctggtgtac gaggaccggg
      601 tgcagatcgt caaggaggtg tcgactgccg agcagcccct cgctgtggct gtggacggcc
      661 acttcctgtg tctggctctg accactcagt acatcatcca caattacagc acaggcgtct
      721 cccaggacct gtttccctac tgcagtgagg agaggccgcc gatcgtcaag aggataggga
      781 gacaggagtt cctgctggcg ggccccggag ggctgggcat gtttgccaca gtcgcaggga
      841 tatcccagcg cgcccccgtg cactggtcgg agaatgtgat tggggcggct gtgtcctttc
      901 catacgtcat agcgctcgat gacgaattca tcacagtcca cagcatgttg gatcagcaac
      961 agaagcagac gctgcccttt aaggagggcc atatcctaca ggactttgaa ggaagagtga
     1021 tcgttgccac aagtaaagga gtttacatct tggttccatt acctttggaa aaacaaatac
     1081 aggatcttct agcaagccgc agagtagaag aggctttggt tttagcaaaa ggagcccgga
     1141 ggaacattcc aaaggaaaaa tttcaggtaa tgtacagaag gattctgcag caggcgggat
     1201 ttatacagtt tgcacaactt cagttcctgg aagctaaaga gctcttcaga agcggccagc
     1261 ttgatgtccg ggagctgatc tctctctacc ccttcctgtt gcccacctcc tcctccttca
     1321 cccggtccca ccctcctctt catgagtacg cagacctgaa ccagctgacc cagggggacc
     1381 aggagaagat ggccaagtgc aaacgcttcc tcatgagcta cctgaacgag gtccgcagca
     1441 cagaggtagc aaatggctac aaggaggaca tcgacacagc cttgctcaaa ctgtatgcag
     1501 aggctgacca cgacagcctg ctggacctcc tggtcactga gaacttctgt cttctgacgg
     1561 acagtgctgc ctggctagag aagcacaaaa agtattttgc acttggactg ctctatcatt
     1621 ataataacca agatgctgct gcagttcagt tgtgggtgaa cattgtgaat ggcgatgtcc
     1681 aggactccac acgctcagac ctgtatgaat acatcgtgga ttttcttacc tactgcttag
     1741 acgaggaact agtgtgggcc tatgctgatt gggtcctgca gaaaagtgaa gaggtcggag
     1801 ttcaggtttt caccaagaga cctttggatg aacagcagaa gaacagtttt aatccagacg
     1861 acattatcaa ttgccttaaa aaatacccta aagcccttgt gaagtatctg gaacatcttg
     1921 tgatagacaa gagactgcag aaagaagagt atcacaccca cttagctgtg ctgtacctgg
     1981 aagaggtgct gctgcagagg gcctccgcca gtggcaaggg tgcagaggcc accgagacgc
     2041 aggccaagct gcggcggctg ctccagaaat ctgatttata ccgagtccac tttcttctcg
     2101 agaggctgca gggagctggc ctgcccatgg agagcgccat cctgcacggg aagctgggcg
     2161 agcatgagaa ggcgctgcat atcctggtgc acgagctgca ggactttgca gcggccgagg
     2221 actactgcct gtggtgctcc gagggccgag acccacccca ccgccagcaa ctctttcaca
     2281 cgctgctggc catctacctg catgctggcc ccactgccca cgagctggcc gtggctgccg
     2341 tggacctgct gaaccgccac gccaccgaat ttgatgcagc ccaggtgctg cagatgctgc
     2401 ctgacacctg gtcagtgcag ctcctctgcc cattcctgat gggggccatg agggacagca
     2461 tccatgccag gaggaccatg caggtggctc tcggcctggc caggtccgaa aacttaatct
     2521 acacctacga taagatgaag ttgaaaggaa gctcaatcca actctcagac aaaaagcttt
     2581 gtcagatatg ccaaaatccc ttttgtgagc ctgtgtttgt tagataccca aatggtggtc
     2641 ttgtgcacac ccactgtgcc gccagcagac acacaaaccc cagctcatcc agtcctggca
     2701 ctcggacttg aaaagcttgg cccaagggtg cgaggggaac tccgagttct ttctgagcct
     2761 gctggacatg aagagcagat gaccaccatg ctctgtgtca gccaggacga agggagacat
     2821 ctgggtgcca gggagccttc cgtccatacg caacaaggac tcctcgctgc tgaccaggct
     2881 ctatgaatgt acatggaatg accaggctct atgaatgtac atggaaaacc cctaaaaatc
     2941 cccaaaacag aaacatacaa gagttcacct gtccaggtac atagtaatcc aagacagagg
     3001 tctactttga aaaaaaaaaa aagggagtct tcctcatgca gtccagaccc aggtggggga
     3061 tcccccacca cccccacact ccaaccttta gactcatgtt tgcactggaa acgtggcttc
     3121 accatggctg gcgacaaagt cctctcttgc ctgggtgagt ggcgagggca cagagcctca
     3181 tgggggtcag ccttgccctc ctgcagaaac acgttctcca tccacgccgc tcactcatgg
     3241 gcccatttcc cagggttacc tcgtgggctc tgcctttgcc ctcagaaacg gctgaccctg
     3301 gagaagtacc tttgttcctt ctttcttcct ctccggctct cagctcccaa aagattctgc
     3361 cttgtgtacc caagcactca ctcttgggtg aactaatgct tctggctgcc tagcaagtga
     3421 agatctgttg agaaactgag aacgctccaa gagagaagct gctctcccct ccttgaagaa
     3481 gccgcggaca cgggaaaccc acggcgggca agggcccagc tgtgtgctcc tttcctgtca
     3541 tggcatctgc tgatttgaaa ctccatgacg aacacgttga actaaacatt tttatttaaa
     3601 tttgtatcat gttcgtagtt gctctcttgt tgagagagtc atctgtcctg tttgttaggg
     3661 gtattttttc ttgttaaaat gtatctcctg ttgaatactc gggaaattca aagggaagtg
     3721 tggtcagcac acactgagcg ttcttttctg gagaggccgg ttttagcctc gtacctttag
     3781 caggaccgac agcgggtgga agggcccgtg tgctggatgg gggtgtcgcc tcctggatgg
     3841 cacaccctcc cgaggacatc cagacaggct gtgtcaccct ccagcacttt atgtgcctgc
     3901 tctgaggtgt cctgaacaca cagcaacggc ctctccttca tcacaggccg gggattcctt
     3961 ttgtgtttct gtataacatc gatgacgagc tgaagctgtt gtcacttcac tgccctcttt
     4021 ggaatttctt cattgtgctg actgattcta ttaggggcta acaataaagg cagtctaccc
     4081 actactatca tgcttgggaa acaagcgtca gcttgtacat atgacaagag aggaccctga
     4141 atacaccctc tcttcatcag cccaagcctt actgaggtgg gatgaagacc tcattctctg
     4201 ttctttaaaa catgtttcgt gaactaatat tggtccatgt attttttgtc cttttgggac
     4261 tctaagtggg tcaagaagca aaaacatcca tgtgttgttt ggggggaatt ggcaccattt
     4321 tttcctgact atgtttcaat taactaaaga ataaatgatg aagaaattct ccttgtagca
     4381 caactgtatg ctgtgtaaat tggtgtgagc tcaaagccca gtcatgtgtt ttacctgtgg
     4441 gtgactctgt tgtagaatgg tcatattact gcatatgtta tcatgacaac ttgtgagttt
     4501 gagtttatat ttctcaaaga acaagttacc cgtatatgca acattgcgcc attaaaagct
     4561 tatttctctg tacacaaaat gggtaaaatc ttgaaaaaac ttagtaattt ctcattccta
     4621 acacacagac agagtctcac tctgtcaccc aggctggagt gcagtggcgc aatctcggct
     4681 cactgcaacc tccacctcct gggttcaagc gattctgctg cctcagcctc ccgagtagct
     4741 gggactacag gtgcatgcca ccacgcccag ctaatttttg tatttttagt agagacgggg
     4801 tttcaccatg ttggccagga tggtctcggt ctcctgacct cgtgatctgc ctgcctcggc
     4861 ctcccaaagt gctgggatta caggcgtgag ccactgcgcc cagccaacac tagtcatttt
     4921 tattttttca ctgaacattg atcaatactc taaaatgttt gtcatccttt agaataatgg
     4981 gaattaaagt tgagcagttt tccatctcta ccaaatcctt caccacaagc agcattctat
     5041 tacagtatga agaattgtct catatgttaa aactgtactt ttctctaacc tttctctcac
     5101 tcctacaagg cccttttctc aaatcatatt tctgagagaa atgtatggtt ctttctcaca
     5161 ggcctgctga tttgtaactg tgccatcgaa tagagcaatt agatccatca gacctttttc
     5221 ccctcattag gttcagtgga accattctgg ttaaaacagc atgaactgaa ctcagtttac
     5281 attcatattc tatgggcaat accatttggt ttttggaact gtttatctgc tgttccctat
     5341 caagtattaa tgtagggatg tcactcaggc tgcgttgcac tgggctgagt tcaactgcga
     5401 cgctctcact gacgcgtgct tacatgtgct tctcacttcc ggagcacagg caggtggctg
     5461 cggggtccca gctctgaatg gatgcccatg atgcaactgg ggcagccctt gctttttgct
     5521 tgctctccca cagaaacctt tgtccttgca actttatcct ttgtcccgat tgggtcccag
     5581 atacacaaaa tgtacaaatg taaacttgct cattttatta aaattctttt agttctttgt
     5641 tctttctgta attttttaaa ataaaaattg ccttgatgga
//
