LOCUS       NM_004612               6492 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens transforming growth factor beta receptor 1 (TGFBR1),
            transcript variant 1, mRNA.
ACCESSION   NM_004612
VERSION     NM_004612.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Huang,W., Zhang,Z., Li,X., Zheng,Q., Wu,C., Liu,L., Chen,Y.,
            Zhang,J. and Jiang,X.
  TITLE     CD9 promotes TbetaR2-TbetaR1 association driving the transition of
            human dermal fibroblasts to myofibroblast under hypoxia
  JOURNAL   Mol Med 30 (1), 162 (2024)
   PUBMED   39333849
  REMARK    GeneRIF: CD9 promotes TbetaR2-TbetaR1 association driving the
            transition of human dermal fibroblasts to myofibroblast under
            hypoxia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Liu,K., Tian,F., Chen,X., Liu,B., Tian,S., Hou,Y., Wang,L., Han,M.,
            Peng,S., Tan,Y., Pan,Y., Chu,Z., Li,J., Che,L., Chen,D., Wen,L.,
            Qin,Z., Li,X., Xiang,J., Bian,X.W., Liu,Q., Ye,X., Wang,T. and
            Wang,B.
  TITLE     Stabilization of TGF-beta Receptor 1 by a Receptor-Associated
            Adaptor Dictates Feedback Activation of the TGF-beta Signaling
            Pathway to Maintain Liver Cancer Stemness and Drug Resistance
  JOURNAL   Adv Sci (Weinh) 11 (34), e2402327 (2024)
   PUBMED   38981014
  REMARK    GeneRIF: Stabilization of TGF-beta Receptor 1 by a
            Receptor-Associated Adaptor Dictates Feedback Activation of the
            TGF-beta Signaling Pathway to Maintain Liver Cancer Stemness and
            Drug Resistance.
REFERENCE   3  (bases 1 to 6492)
  AUTHORS   Cui,N., Liu,C., Tang,X., Song,L., Xiao,Z., Wang,C., Wu,Y., Zhou,Y.,
            Peng,C., Liu,Y., Zheng,L., Liu,X., Huang,K. and Chen,H.
  TITLE     ISG15 accelerates acute kidney injury and the subsequent AKI-to-CKD
            transition by promoting TGFbetaR1 ISGylation
  JOURNAL   Theranostics 14 (11), 4536-4553 (2024)
   PUBMED   39113797
  REMARK    GeneRIF: ISG15 accelerates acute kidney injury and the subsequent
            AKI-to-CKD transition by promoting TGFbetaR1 ISGylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6492)
  AUTHORS   Du,Q., Zhang,D., Zhuang,Y., Xia,Q., Wen,T. and Jia,H.
  TITLE     The Molecular Genetics of Marfan Syndrome
  JOURNAL   Int J Med Sci 18 (13), 2752-2766 (2021)
   PUBMED   34220303
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6492)
  AUTHORS   Tzavlaki,K. and Moustakas,A.
  TITLE     TGF-beta Signaling
  JOURNAL   Biomolecules 10 (3), 487 (2020)
   PUBMED   32210029
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6492)
  AUTHORS   Vander Ark,A., Cao,J. and Li,X.
  TITLE     TGF-beta receptors: In and beyond TGF-beta signaling
  JOURNAL   Cell Signal 52, 112-120 (2018)
   PUBMED   30184463
  REMARK    Review article
REFERENCE   7  (bases 1 to 6492)
  AUTHORS   Loeys,B.L. and Dietz,H.C.
  TITLE     Loeys-Dietz Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301312
REFERENCE   8  (bases 1 to 6492)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
REFERENCE   9  (bases 1 to 6492)
  AUTHORS   Cheifetz,S., Bellon,T., Cales,C., Vera,S., Bernabeu,C., Massague,J.
            and Letarte,M.
  TITLE     Endoglin is a component of the transforming growth factor-beta
            receptor system in human endothelial cells
  JOURNAL   J Biol Chem 267 (27), 19027-19030 (1992)
   PUBMED   1326540
REFERENCE   10 (bases 1 to 6492)
  AUTHORS   Massague,J.
  TITLE     Receptors for the TGF-beta family
  JOURNAL   Cell 69 (7), 1067-1070 (1992)
   PUBMED   1319842
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK302059.1, L11695.1 and
            AJ619019.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004612.3.
            
            Summary: The protein encoded by this gene forms a heteromeric
            complex with type II TGF-beta receptors when bound to TGF-beta,
            transducing the TGF-beta signal from the cell surface to the
            cytoplasm. The encoded protein is a serine/threonine protein
            kinase. Mutations in this gene have been associated with
            Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Aug 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.227512.1,
                                           SRR1803613.20696.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374994.9/ ENSP00000364133.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AK302059.1         25-41
            18-1673             L11695.1           1-1656
            1674-6492           AJ619019.2         1669-6487
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.33"
     gene            1..6492
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="transforming growth factor beta receptor 1"
                     /db_xref="GeneID:7046"
                     /db_xref="HGNC:HGNC:11772"
                     /db_xref="MIM:190181"
     exon            1..190
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..1605
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /EC_number="2.7.11.30"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; activin A receptor type II-like kinase, 53kDa;
                     TGF-beta receptor type-1; activin receptor-like kinase 5;
                     serine/threonine-protein kinase receptor R4; transforming
                     growth factor beta receptor I; transforming growth
                     factor-beta receptor type I; activin A receptor type
                     II-like protein kinase of 53kD; mutant transforming growth
                     factor beta receptor I"
                     /codon_start=1
                     /product="TGF-beta receptor type-1 isoform 1 precursor"
                     /protein_id="NP_004603.1"
                     /db_xref="CCDS:CCDS6738.1"
                     /db_xref="GeneID:7046"
                     /db_xref="HGNC:HGNC:11772"
                     /db_xref="MIM:190181"
                     /translation="MEAAVAAPRPRLLLLVLAAAAAAAAALLPGATALQCFCHLCTKD
                     NFTCVTDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGSVTTTYCCN
                     QDHCNKIELPTTVKSSPGLGPVELAAVIAGPVCFVCISLMLMVYICHNRTVIHHRVPN
                     EEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGE
                     VWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQL
                     WLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDL
                     KSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKH
                     FESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKL
                     RPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM"
     exon            191..436
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..667
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            668..898
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1066
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1223
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1348
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1479
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..6492
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5929..5934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="hexamer: AATAAA"
     polyA_site      5949
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="major polyA site"
     regulatory      5970..5975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="hexamer: AATAAA"
     polyA_site      5990
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
     regulatory      6358..6363
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="hexamer: ATTAAA"
     polyA_site      6383
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
     regulatory      6471..6476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
                     /note="hexamer: ATTAAA"
     polyA_site      6492
                     /gene="TGFBR1"
                     /gene_synonym="AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1;
                     LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1"
ORIGIN      
        1 ggcggctagg gaggtggggc gaggcgaggt ttgctggggt gaggcagcgg cgcggccggg
       61 ccgggccggg ccacaggcgg tggcggcggg accatggagg cggcggtcgc tgctccgcgt
      121 ccccggctgc tcctcctcgt gctggcggcg gcggcggcgg cggcggcggc gctgctcccg
      181 ggggcgacgg cgttacagtg tttctgccac ctctgtacaa aagacaattt tacttgtgtg
      241 acagatgggc tctgctttgt ctctgtcaca gagaccacag acaaagttat acacaacagc
      301 atgtgtatag ctgaaattga cttaattcct cgagataggc cgtttgtatg tgcaccctct
      361 tcaaaaactg ggtctgtgac tacaacatat tgctgcaatc aggaccattg caataaaata
      421 gaacttccaa ctactgtaaa gtcatcacct ggccttggtc ctgtggaact ggcagctgtc
      481 attgctggac cagtgtgctt cgtctgcatc tcactcatgt tgatggtcta tatctgccac
      541 aaccgcactg tcattcacca tcgagtgcca aatgaagagg acccttcatt agatcgccct
      601 tttatttcag agggtactac gttgaaagac ttaatttatg atatgacaac gtcaggttct
      661 ggctcaggtt taccattgct tgttcagaga acaattgcga gaactattgt gttacaagaa
      721 agcattggca aaggtcgatt tggagaagtt tggagaggaa agtggcgggg agaagaagtt
      781 gctgttaaga tattctcctc tagagaagaa cgttcgtggt tccgtgaggc agagatttat
      841 caaactgtaa tgttacgtca tgaaaacatc ctgggattta tagcagcaga caataaagac
      901 aatggtactt ggactcagct ctggttggtg tcagattatc atgagcatgg atcccttttt
      961 gattacttaa acagatacac agttactgtg gaaggaatga taaaacttgc tctgtccacg
     1021 gcgagcggtc ttgcccatct tcacatggag attgttggta cccaaggaaa gccagccatt
     1081 gctcatagag atttgaaatc aaagaatatc ttggtaaaga agaatggaac ttgctgtatt
     1141 gcagacttag gactggcagt aagacatgat tcagccacag ataccattga tattgctcca
     1201 aaccacagag tgggaacaaa aaggtacatg gcccctgaag ttctcgatga ttccataaat
     1261 atgaaacatt ttgaatcctt caaacgtgct gacatctatg caatgggctt agtattctgg
     1321 gaaattgctc gacgatgttc cattggtgga attcatgaag attaccaact gccttattat
     1381 gatcttgtac cttctgaccc atcagttgaa gaaatgagaa aagttgtttg tgaacagaag
     1441 ttaaggccaa atatcccaaa cagatggcag agctgtgaag ccttgagagt aatggctaaa
     1501 attatgagag aatgttggta tgccaatgga gcagctaggc ttacagcatt gcggattaag
     1561 aaaacattat cgcaactcag tcaacaggaa ggcatcaaaa tgtaattcta cagctttgcc
     1621 tgaactctcc ttttttcttc agatctgctc ctgggtttta atttgggagg tcaattgttc
     1681 tacctcactg agagggaaca gaaggatatt gcttcctttt gcagcagtgt aataaagtca
     1741 attaaaaact tcccaggatt tctttggacc caggaaacag ccatgtgggt cctttctgtg
     1801 cactatgaac gcttctttcc caggacagaa aatgtgtagt ctacctttat tttttattaa
     1861 caaaacttgt tttttaaaaa gatgattgct ggtcttaact ttaggtaact ctgctgtgct
     1921 ggagatcatc tttaagggca aaggagttgg attgctgaat tacaatgaaa catgtcttat
     1981 tactaaagaa agtgatttac tcctggttag tacattctca gaggattctg aaccactaga
     2041 gtttccttga ttcagacttt gaatgtactg ttctatagtt tttcaggatc ttaaaactaa
     2101 cacttataaa actcttatct tgagtctaaa aatgacctca tatagtagtg aggaacataa
     2161 ttcatgcaat tgtattttgt atactattat tgttctttca cttattcaga acattacatg
     2221 ccttcaaaat gggattgtac tataccagta agtgccactt ctgtgtcttt ctaatggaaa
     2281 tgagtagaat tgctgaaagt ctctatgtta aaacctatag tgtttgaatt caaaaagctt
     2341 atttatctgg gtaacccaaa ctttttctgt tttgtttttg gaagggtttt tgtggtatgt
     2401 catttggtat tctattctga aaatgccttt ctcctaccaa aatgtgctta agccactaaa
     2461 gaaatgaagt ggcattaatt agtaaattat tagcatggtc atgtttgaat attctcacat
     2521 caagcttttg cattttaatt gtgttgtcta agtatacttt taaaaaatca agtggcactc
     2581 tagatgctta tagtacttta atatttgtag catacagact aatttttcta aaagggaaag
     2641 tctgtctagc tgcttgtgaa aagttatgtg gtattctgta agccattttt ttctttatct
     2701 gttcaaagac ttatttttta agacatgaat tacatttaaa attagaatat ggttaatatt
     2761 aaataatagg cctttttcta ggaaggcgaa ggtagttaat aatttgaata gataacagat
     2821 gtgcaagaaa gtcacatttg ttatgtatgt aggagtaaac gttcggtgga tcctctgtct
     2881 ttgtaactga ggttagagct agtgtggttt tgaggtctca ctacactttg aggaaggcag
     2941 cttttaattc agtgtttcct tatgtgtgcg tacattgcaa ctgcttacat gtaatttatg
     3001 taatgcattc agtgcaccct tgttacttgg gagaggtggt agctaaagaa cattctgagt
     3061 ataggttttt ctccatttac agatgtcttt ggtcaaatat tgaaagcaaa cttgtcatgg
     3121 tcttcttaca ttaagttgaa actagcttat aataactggt ttttacttcc aatgctatga
     3181 agtctctgca gggcttttac agttttcgaa gtccttttat cactgtgatc ttattctgag
     3241 gggagaaaaa actatcatag ctctgaggca agacttcgac tttatagtgc tatcagttcc
     3301 ccgatacagg gtcagagtaa cccatacagt attttggtca ggaagagaaa gtggccattt
     3361 acactgaatg agttgcattc tgataatgtc ttatctctta tacgtagaat aaatttgaaa
     3421 gactatttga tcttaaaacc aaagtaattt tagaatgagt gacatattac ataggaattt
     3481 agtgtcaatt tcatgtgttt aaaaacatca tgggaaaaat gcttagaggt tactattttg
     3541 actacaaagt tgagtttttt tctgtagtta ccataatttc attgaagcaa atgaatgagt
     3601 ttgagaggtt tgtttttata gttgtgttgt attacttgtt taataataat ctctaattct
     3661 gtgatcaggt actttttttg tgggggtttt ttttttgttt tttttttttt gttgttgttt
     3721 ttgggccatt tctaagccta ccagatctgc tttatgaaat ccaggggacc aatgcatttt
     3781 atcactaaaa ctatttttat ataattttaa gaatatacca aaagttgtct gatttaaagt
     3841 tgtaatacat gatttctcac tttcatgtaa ggttatccac ttttgctgaa gatatttttt
     3901 attgaatcaa agattgagtt acaattatac ttttcttacc taagtggata aaatgtactt
     3961 ttgatgaatc agggaatttt tttaaagttg gagtttagtt ctaaattgac tttacgtatt
     4021 actgcagtta attccttttt tggctaggga tggtttgata aaccacaatt ggctgatatt
     4081 gaaaatgaaa gaaacttaaa aggtgggatg gatcatgatt actgtcgata actgcagata
     4141 aatttgatta gagtaataat tttgtcattt aaaaacacag ttgtttatac tgcccatcct
     4201 aggatgctca ccttccaaga ttcaacgtgg ctaaaacatc ttctggtaaa ttgtgcgtcc
     4261 atattcattt tgtcagtagc caggagaaat ggggatgggg gaaatacgac ttagtgaggc
     4321 atagacatcc ctggtccatc ctttctgtct ccagctgttt cttggaacct gctctcctgc
     4381 ttgctggtcc ctgacgcaga gaccgttgcc tcccccacag ccgtttgact gaaggctgct
     4441 ctggagacct agagtaaaac ggctgatgga agttgtggga cccacttcca tttccttcag
     4501 tcattagagg tggaagggag gggtctccaa gtttggagat tgagcagatg aggcttggga
     4561 tgcccctgct ttgacttcag ccatggatga ggagtgggat ggcagcaagg tggctcctgt
     4621 ggcagtggag ttgtgccaga aacagtggcc agttgtatcg cctataagac agggtaaggt
     4681 ctgaagagct gagcctgtaa ttctgctgta ataatgatag tgctcaagaa gtgccttgag
     4741 ttggtgtaca gtgccatggc catcaagaat cccagatttc aggttttatt acaaaatgta
     4801 agtggtcact tggcgatttt gtagtacatg catgagttac cttttttctc tatgtctgag
     4861 aactgtcaga ttaaaacaag atggcaaaga gatcgttaga gtgcacaaca aaatcactat
     4921 cccattagac acatcatcaa aagcttattt ttattcttgc actggaagaa tcgtaagtca
     4981 actgtttctt gaccatggca gtgttctggc tccaaatggt agtgattcca aataatggtt
     5041 ctgttaacac tttggcagaa aatgccagct cagatatttt gagatactaa ggattatctt
     5101 tggacatgta ctgcagcttc ttgtctctgt tttggattac tggaataccc atgggccctc
     5161 tcaagagtgc tggacttcta ggacattaag atgattgtca gtacattaaa cttttcaatc
     5221 ccattatgca atcttgtttg taaatgtaaa cttctaaaaa tatggttaat aacattcaac
     5281 ctgtttatta caacttaaaa ggaacttcag tgaatttgtt tttatttttt aacaagattt
     5341 gtgaactgaa tatcatgaac catgttttga tacccctttt tcacgttgtg ccaacggaat
     5401 agggtgtttg atatttcttc atatgttaag gagatgcttc aaaatgtcaa ttgctttaaa
     5461 cttaaattac ctctcaagag accaaggtac atttacctca ttgtgtatat aatgtttaat
     5521 atttgtcaga gcattctcca ggtttgcagt tttatttcta taaagtatgg gtattatgtt
     5581 gctcagttac tcaaatggta ctgtattgtt tatatttgta ccccaaataa catcgtctgt
     5641 actttctgtt ttctgtattg tatttgtgca ggattcttta ggctttatca gtgtaatctc
     5701 tgccttttaa gatatgtaca gaaaatgtcc atataaattt ccattgaagt cgaatgatac
     5761 tgagaagcct gtaaagagga gaaaaaaaca taagctgtgt ttccccataa gtttttttaa
     5821 attgtatatt gtatttgtag taatattcca aaagaatgta aataggaaat agaagagtga
     5881 tgcttatgtt aagtcctaac actacagtag aagaatggaa gcagtgcaaa taaattacat
     5941 ttttcccaag tgccagtggc atattttaaa ataaagtgta tacgttggaa tgagtcatgc
     6001 catatgtagt tgctgtagat ggcaactaga acctttgagt tacaagagtc tttagaagtt
     6061 ttctaaccct gcctagtgca agttacaata ttatagcgtg ttcggggagt gccctcctgt
     6121 ctgcaggtgt gtctctgtgc ctgggggctt ttctccacat gcttaggggt gtgggtcttc
     6181 cattggggca tgatggacct gtctacaggt gatctctgtt gcctttgggt cagcacattt
     6241 gttagtctcc tgggggtgaa aacttggctt acaagagaac tggaaaaatg atgagatgtg
     6301 gtccccaaac ccttgattga ctctggggag gggctttgtg aataggattg ctctcacatt
     6361 aaagatagtt acttcaattt gaaggctgga tttagggatt tttttttttc cttataacaa
     6421 agacatcacc aggatatgaa gcttttgttg aaagttggaa aaaaagtgaa attaaagaca
     6481 ttcccagaca aa
//
