LOCUS       NM_003235               8455 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens thyroglobulin (TG), mRNA.
ACCESSION   NM_003235
VERSION     NM_003235.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Kargun,S., Aydemir,M., Yilmaz,N., Ozdem,S. and Sari,R.
  TITLE     Diagnostic value of thyroglobulin washout in fine-needle aspiration
            samples for diagnosis and follow-up of differentiated thyroid
            cancer
  JOURNAL   Medicine (Baltimore) 102 (49), e36426 (2023)
   PUBMED   38065908
  REMARK    GeneRIF: Diagnostic value of thyroglobulin washout in fine-needle
            aspiration samples for diagnosis and follow-up of differentiated
            thyroid cancer.
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Hei,H., Luo,Z., Zheng,C., Gong,W., Zhou,B., Fang,J. and Qin,J.
  TITLE     Lymph node ratio independently associated with postoperative
            thyroglobulin levels in papillary thyroid cancer
  JOURNAL   Oral Oncol 146, 106563 (2023)
   PUBMED   37690364
  REMARK    GeneRIF: Lymph node ratio independently associated with
            postoperative thyroglobulin levels in papillary thyroid cancer.
REFERENCE   3  (bases 1 to 8455)
  AUTHORS   Gomes Pio,M., Adrover,E., Miras,M.B., Sobrero,G., Molina,M.F.,
            Scheps,K.G., Rivolta,C.M. and Targovnik,H.M.
  TITLE     The p.Cys1281Tyr variant in the hinge module/flap region of
            thyroglobulin causes intracellular transport disorder and
            congenital hypothyroidism
  JOURNAL   Mol Cell Endocrinol 572, 111948 (2023)
   PUBMED   37164149
  REMARK    GeneRIF: The p.Cys1281Tyr variant in the hinge module/flap region
            of thyroglobulin causes intracellular transport disorder and
            congenital hypothyroidism.
REFERENCE   4  (bases 1 to 8455)
  AUTHORS   Du,Y., Liu,P., Zhang,W., Yao,J., Meng,F., Fan,L., Liu,L., Li,M.,
            Lv,C., Jiang,W. and Sun,D.
  TITLE     Serum thyroglobulin as a biomarker of iodine excess and thyroid
            disease occurrence in adults
  JOURNAL   J Trace Elem Med Biol 78, 127172 (2023)
   PUBMED   37043922
  REMARK    GeneRIF: Serum thyroglobulin as a biomarker of iodine excess and
            thyroid disease occurrence in adults.
REFERENCE   5  (bases 1 to 8455)
  AUTHORS   Fuenzalida-Mery,L., Gonzalez-Arestizabal,T., Portilla-Rojas,M.,
            Gac-Espinoza,P., Rodriguez-Moreno,F., CabaneToledo,P. and
            Rappoport-Wurgaft,D.
  TITLE     [Analysis of pre-ablative and preoperative thyroglobulin in
            differentiated thyroid cancer]
  JOURNAL   Rev Med Chil 150 (7), 855-860 (2022)
   PUBMED   37906818
  REMARK    GeneRIF: [Analysis of pre-ablative and preoperative thyroglobulin
            in differentiated thyroid cancer].
REFERENCE   6  (bases 1 to 8455)
  AUTHORS   Targovnik,H.M., Cochaux,P., Corach,D. and Vassart,G.
  TITLE     Identification of a minor Tg mRNA transcript in RNA from normal and
            goitrous thyroids
  JOURNAL   Mol Cell Endocrinol 84 (1-2), R23-R26 (1992)
   PUBMED   1639210
REFERENCE   7  (bases 1 to 8455)
  AUTHORS   Henry,M., Zanelli,E., Piechaczyk,M., Pau,B. and Malthiery,Y.
  TITLE     A major human thyroglobulin epitope defined with monoclonal
            antibodies is mainly recognized by human autoantibodies
  JOURNAL   Eur J Immunol 22 (2), 315-319 (1992)
   PUBMED   1371467
REFERENCE   8  (bases 1 to 8455)
  AUTHORS   Dunn,A.D., Crutchfield,H.E. and Dunn,J.T.
  TITLE     Thyroglobulin processing by thyroidal proteases. Major sites of
            cleavage by cathepsins B, D, and L
  JOURNAL   J Biol Chem 266 (30), 20198-20204 (1991)
   PUBMED   1939080
REFERENCE   9  (bases 1 to 8455)
  AUTHORS   Lamas,L., Anderson,P.C., Fox,J.W. and Dunn,J.T.
  TITLE     Consensus sequences for early iodination and hormonogenesis in
            human thyroglobulin
  JOURNAL   J Biol Chem 264 (23), 13541-13545 (1989)
   PUBMED   2760035
REFERENCE   10 (bases 1 to 8455)
  AUTHORS   Marriq,C., Lejeune,P.J., Venot,N. and Vinet,L.
  TITLE     Hormone synthesis in human thyroglobulin: possible cleavage of the
            polypeptide chain at the tyrosine donor site
  JOURNAL   FEBS Lett 242 (2), 414-418 (1989)
   PUBMED   2914619
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF230666.2, X05615.1, U93033.2,
            AU142151.1, AU141923.1, AU141607.1 and AU141420.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003235.4.
            
            Summary: Thyroglobulin (Tg) is a glycoprotein homodimer produced
            predominantly by the thryroid gland. It acts as a substrate for the
            synthesis of thyroxine and triiodothyronine as well as the storage
            of the inactive forms of thyroid hormone and iodine. Thyroglobulin
            is secreted from the endoplasmic reticulum to its site of
            iodination, and subsequent thyroxine biosynthesis, in the
            follicular lumen. Mutations in this gene cause thyroid
            dyshormonogenesis, manifested as goiter, and are associated with
            moderate to severe congenital hypothyroidism. Polymorphisms in this
            gene are associated with susceptibility to autoimmune thyroid
            diseases (AITD) such as Graves disease and Hashimoto thryoiditis.
            [provided by RefSeq, Nov 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X05615.1, U93033.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220616.9/ ENSP00000220616.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AF230666.2         65807-65808
            3-120               X05615.1           1-118
            121-2242            U93033.2           78-2199
            2243-2802           X05615.1           2241-2800
            2803-3124           U93033.2           2760-3081
            3125-3381           X05615.1           3120-3376
            3382-3516           U93033.2           3339-3473
            3517-3677           AU142151.1         341-501
            3678-4045           AU141923.1         96-463
            4046-4548           U93033.2           4003-4505
            4549-4571           AU141607.1         597-619
            4572-5554           U93033.2           4529-5511
            5555-5591           AU141420.1         609-645
            5592-7447           U93033.2           5549-7404
            7448-8419           X05615.1           7443-8414
            8420-8455           U93033.2           8377-8412
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.22"
     gene            1..8455
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /note="thyroglobulin"
                     /db_xref="GeneID:7038"
                     /db_xref="HGNC:HGNC:11764"
                     /db_xref="MIM:188450"
     exon            1..110
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..8350
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /codon_start=1
                     /product="thyroglobulin precursor"
                     /protein_id="NP_003226.4"
                     /db_xref="CCDS:CCDS34944.1"
                     /db_xref="GeneID:7038"
                     /db_xref="HGNC:HGNC:11764"
                     /db_xref="MIM:188450"
                     /translation="MALVLEIFTLLASICWVSANIFEYQVDAQPLRPCELQRETAFLK
                     QADYVPQCAEDGSFQTVQCQNDGRSCWCVGANGSEVLGSRQPGRPVACLSFCQLQKQQ
                     ILLSGYINSTDTSYLPQCQDSGDYAPVQCDVQQVQCWCVDAEGMEVYGTRQLGRPKRC
                     PRSCEIRNRRLLHGVGDKSPPQCSAEGEFMPVQCKFVNTTDMMIFDLVHSYNRFPDAF
                     VTFSSFQRRFPEVSGYCHCADSQGRELAETGLELLLDEIYDTIFAGLDLPSTFTETTL
                     YRILQRRFLAVQSVISGRFRCPTKCEVERFTATSFGHPYVPSCRRNGDYQAVQCQTEG
                     PCWCVDAQGKEMHGTRQQGEPPSCAEGQSCASERQQALSRLYFGTSGYFSQHDLFSSP
                     EKRWASPRVARFATSCPPTIKELFVDSGLLRPMVEGQSQQFSVSENLLKEAIRAIFPS
                     RGLARLALQFTTNPKRLQQNLFGGKFLVNVGQFNLSGALGTRGTFNFSQFFQQLGLAS
                     FLNGGRQEDLAKPLSVGLDSNSSTGTPEAAKKDGTMNKPTVGSFGFEINLQENQNALK
                     FLASLLELPEFLLFLQHAISVPEDVARDLGDVMETVLSSQTCEQTPERLFVPSCTTEG
                     SYEDVQCFSGECWCVNSWGKELPGSRVRGGQPRCPTDCEKQRARMQSLMGSQPAGSTL
                     FVPACTSEGHFLPVQCFNSECYCVDAEGQAIPGTRSAIGKPKKCPTPCQLQSEQAFLR
                     TVQALLSNSSMLPTLSDTYIPQCSTDGQWRQVQCNGPPEQVFELYQRWEAQNKGQDLT
                     PAKLLVKIMSYREAASGNFSLFIQSLYEAGQQDVFPVLSQYPSLQDVPLAALEGKRPQ
                     PRENILLEPYLFWQILNGQLSQYPGSYSDFSTPLAHFDLRNCWCVDEAGQELEGMRSE
                     PSKLPTCPGSCEEAKLRVLQFIRETEEIVSASNSSRFPLGESFLVAKGIRLRNEDLGL
                     PPLFPPREAFAEQFLRGSDYAIRLAAQSTLSFYQRRRFSPDDSAGASALLRSGPYMPQ
                     CDAFGSWEPVQCHAGTGHCWCVDEKGGFIPGSLTARSLQIPQCPTTCEKSRTSGLLSS
                     WKQARSQENPSPKDLFVPACLETGEYARLQASGAGTWCVDPASGEELRPGSSSSAQCP
                     SLCNVLKSGVLSRRVSPGYVPACRAEDGGFSPVQCDQAQGSCWCVMDSGEEVPGTRVT
                     GGQPACESPRCPLPFNASEVVGGTILCETISGPTGSAMQQCQLLCRQGSWSVFPPGPL
                     ICSLESGRWESQLPQPRACQRPQLWQTIQTQGHFQLQLPPGKMCSADYADLLQTFQVF
                     ILDELTARGFCQIQVKTFGTLVSIPVCNNSSVQVGCLTRERLGVNVTWKSRLEDIPVA
                     SLPDLHDIERALVGKDLLGRFTDLIQSGSFQLHLDSKTFPAETIRFLQGDHFGTSPRT
                     WFGCSEGFYQVLTSEASQDGLGCVKCPEGSYSQDEECIPCPVGFYQEQAGSLACVPCP
                     VGRTTISAGAFSQTHCVTDCQRNEAGLQCDQNGQYRASQKDRGSGKAFCVDGEGRRLP
                     WWETEAPLEDSQCLMMQKFEKVPESKVIFDANAPVAVRSKVPDSEFPVMQCLTDCTED
                     EACSFFTVSTTEPEISCDFYAWTSDNVACMTSDQKRDALGNSKATSFGSLRCQVKVRS
                     HGQDSPAVYLKKGQGSTTTLQKRFEPTGFQNMLSGLYNPIVFSASGANLTDAHLFCLL
                     ACDRDLCCDGFVLTQVQGGAIICGLLSSPSVLLCNVKDWMDPSEAWANATCPGVTYDQ
                     ESHQVILRLGDQEFIKSLTPLEGTQDTFTNFQQVYLWKDSDMGSRPESMGCRKDTVPR
                     PASPTEAGLTTELFSPVDLNQVIVNGNQSLSSQKHWLFKHLFSAQQANLWCLSRCVQE
                     HSFCQLAEITESASLYFTCTLYPEAQVCDDIMESNAQGCRLILPQMPKALFRKKVILE
                     DKVKNFYTRLPFQKLMGISIRNKVPMSEKSISNGFFECERRCDADPCCTGFGFLNVSQ
                     LKGGEVTCLTLNSLGIQMCSEENGGAWRILDCGSPDIEVHTYPFGWYQKPIAQNNAPS
                     FCPLVVLPSLTEKVSLDSWQSLALSSVVVDPSIRHFDVAHVSTAATSNFSAVRDLCLS
                     ECSQHEACLITTLQTQPGAVRCMFYADTQSCTHSLQGQNCRLLLREEATHIYRKPGIS
                     LLSYEASVPSVPISTHGRLLGRSQAIQVGTSWKQVDQFLGVPYAAPPLAERRFQAPEP
                     LNWTGSWDASKPRASCWQPGTRTSTSPGVSEDCLYLNVFIPQNVAPNASVLVFFHNTM
                     DREESEGWPAIDGSFLAAVGNLIVVTASYRVGVFGFLSSGSGEVSGNWGLLDQVAALT
                     WVQTHIRGFGGDPRRVSLAADRGGADVASIHLLTARATNSQLFRRAVLMGGSALSPAA
                     VISHERAQQQAIALAKEVSCPMSSSQEVVSCLRQKPANVLNDAQTKLLAVSGPFHYWG
                     PVIDGHFLREPPARALKRSLWVEVDLLIGSSQDDGLINRAKAVKQFEESRGRTSSKTA
                     FYQALQNSLGGEDSDARVEAAATWYYSLEHSTDDYASFSRALENATRDYFIICPIIDM
                     ASAWAKRARGNVFMYHAPENYGHGSLELLADVQFALGLPFYPAYEGQFSLEEKSLSLK
                     IMQYFSHFIRSGNPNYPYEFSRKVPTFATPWPDFVPRAGGENYKEFSELLPNRQGLKK
                     ADCSFWSKYISSLKTSADGAKGGQSAESEEEELTAGSGLREDLLSLQEPGSKTYSK"
     exon            111..219
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            220..317
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            318..521
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            522..681
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            682..788
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            789..932
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1118
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..2219
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2804
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..3044
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3182
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3260
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3261..3373
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3374..3476
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3677
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3678..3890
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3891..4045
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4046..4202
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4421
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4422..4571
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4572..4742
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4743..4859
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4860..4975
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            4976..5084
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5085..5276
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5277..5444
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5445..5510
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5511..5591
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5592..5729
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5730..5906
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            5907..6018
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6019..6098
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6099..6242
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6243..6305
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6306..6440
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6441..6605
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6606..6825
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6826..6919
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            6920..7079
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7080..7282
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7283..7447
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7448..7615
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7616..7797
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7798..7905
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            7906..8040
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            8041..8231
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     exon            8232..8455
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8432..8437
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /note="hexamer: AATAAA"
     polyA_site      8455
                     /gene="TG"
                     /gene_synonym="AITD3; TGN"
                     /note="major polyA site"
ORIGIN      
        1 aagcagtggt ttctcctcct tcctcccagg aagggccagg aaaatggccc tggtcctgga
       61 gatcttcacc ctgctggcct ccatctgctg ggtgtcggcc aatatcttcg agtaccaggt
      121 ggatgcccag ccccttcgtc cctgtgagct gcagagggaa acggcctttc tgaagcaagc
      181 agactacgtg ccccagtgtg cagaggatgg cagcttccag actgtccagt gccagaacga
      241 cggccgctcc tgctggtgtg tgggtgccaa cggcagtgaa gtgctgggca gcaggcagcc
      301 aggacggcct gtggcttgtc tgtcattttg tcagctacag aaacagcaga tcttactgag
      361 tggctacatt aacagcacag acacctccta cctccctcag tgtcaggatt caggggacta
      421 cgcgcctgtt cagtgtgatg tgcagcaggt ccagtgctgg tgtgtggacg cagaggggat
      481 ggaggtgtat gggacccgcc agctggggag gccaaagcga tgtccaagga gctgtgaaat
      541 aagaaatcgt cgtcttctcc acggggtggg agataagtca ccaccccagt gttctgcgga
      601 gggagagttt atgcctgtcc agtgcaaatt tgtcaacacc acagacatga tgatttttga
      661 tctggtccac agctacaaca ggtttccaga tgcatttgtg accttcagtt ccttccagag
      721 gaggttccct gaggtatctg ggtattgcca ctgtgctgac agccaagggc gggaactggc
      781 tgagacaggt ttggagttgt tactggatga aatttatgac accatttttg ctggcctgga
      841 ccttccttcc accttcactg aaaccaccct gtaccggata ctgcagagac ggttcctcgc
      901 agttcaatca gtcatctctg gcagattccg atgccccaca aaatgtgaag tggagcggtt
      961 tacagcaacc agctttggtc acccctatgt tccaagctgc cgccgaaatg gcgactatca
     1021 ggcggtgcag tgccagacgg aagggccctg ctggtgtgtg gacgcccagg ggaaggaaat
     1081 gcatggaacc cggcagcaag gggagccgcc atcttgtgct gaaggccaat cttgtgcctc
     1141 cgaaaggcag caggccttgt ccagactcta ctttgggacc tcaggctact tcagccagca
     1201 cgacctgttc tcttccccag agaaaagatg ggcctctcca agagtagcca gatttgccac
     1261 atcctgccca cccacgatca aggagctctt tgtggactct gggcttctcc gcccaatggt
     1321 ggagggacag agccaacagt tttctgtctc agaaaatctt ctcaaagaag ccatccgagc
     1381 aatttttccc tcccgagggc tggctcgtct tgcccttcag tttaccacca acccaaagag
     1441 actccagcaa aacctttttg gagggaaatt tttggtgaat gttggccagt ttaacttgtc
     1501 tggagccctt ggcacaagag gcacatttaa cttcagtcaa tttttccagc aacttggtct
     1561 tgcaagcttc ttgaatggag ggagacaaga agatttggcc aagccactct ctgtgggatt
     1621 agattcaaat tcttccacag gaacccctga agctgctaag aaggatggta ctatgaataa
     1681 gccaactgtg ggcagctttg gctttgaaat taacctacaa gagaaccaaa atgccctcaa
     1741 attccttgct tctctcctgg agcttccaga attccttctc ttcttgcaac atgctatctc
     1801 tgtgccagaa gatgtggcaa gagatttagg tgatgtgatg gaaacggtac tcagctccca
     1861 gacctgtgag cagacacctg aaaggctatt tgtcccatca tgcacgacag aaggaagcta
     1921 tgaggatgtc caatgctttt ccggagagtg ctggtgtgtg aattcctggg gcaaagagct
     1981 tccaggctca agagtcagag gtggacagcc aaggtgcccc acagactgtg aaaagcaaag
     2041 ggctcgcatg caaagcctca tgggcagcca gcctgctggc tccaccttgt ttgtccctgc
     2101 ttgtactagt gagggacatt tcctgcctgt ccagtgcttc aactcagagt gctactgtgt
     2161 tgatgctgag ggtcaggcca ttcctggaac tcgaagtgca atagggaagc ccaagaaatg
     2221 ccccacgccc tgtcaattac agtctgagca agctttcctc aggacggtgc aggccctgct
     2281 ctctaactcc agcatgctac ccaccctttc cgacacctac atcccacagt gcagcaccga
     2341 tgggcagtgg agacaagtgc aatgcaatgg gcctcctgag caggtcttcg agttgtacca
     2401 acgatgggag gctcagaaca agggccagga tctgacgcct gccaagctgc tagtgaagat
     2461 catgagctac agagaagcag cttccggaaa cttcagtctc tttattcaaa gtctgtatga
     2521 ggctggccag caagatgtct tcccggtgct gtcacaatac ccttctctgc aagatgtccc
     2581 actagcagca ctggaaggga aacggcccca gcccagggag aatatcctcc tggagcccta
     2641 cctcttctgg cagatcttaa atggccaact cagccaatac ccggggtcct actcagactt
     2701 cagcactcct ttggcacatt ttgatcttcg gaactgctgg tgtgtggatg aggctggcca
     2761 agaactggaa ggaatgcggt ctgagccaag caagctccca acatgtcctg gctcctgtga
     2821 ggaagcaaag ctccgtgtac tgcagttcat tagggaaacg gaagagattg tttcagcttc
     2881 caacagttct cggttccctc tgggggagag tttcctggtg gccaagggaa tccggctgag
     2941 gaatgaggac ctcggccttc ctccgctctt cccgccccgg gaggctttcg cggagcagtt
     3001 tctgcgtggg agtgattacg ccattcgcct ggcggctcag tctaccttaa gcttctatca
     3061 gagacgccgc ttttccccgg acgactcggc tggagcatcc gcccttctgc ggtcgggccc
     3121 ctacatgcca cagtgtgatg cgtttggaag ttgggagcct gtgcagtgcc acgctgggac
     3181 tgggcactgc tggtgtgtag atgagaaagg agggttcatc cctggctcac tgactgcccg
     3241 ctctctgcag attccacagt gcccgacaac ctgcgagaaa tctcgaacca gtgggctgct
     3301 ttccagttgg aaacaggcta gatcccaaga aaacccatct ccaaaagacc tgttcgtccc
     3361 agcctgccta gaaacaggag agtatgccag gctgcaggca tcgggggctg gcacctggtg
     3421 tgtggaccct gcatcaggag aagagttgcg gcctggctcg agcagcagtg cccagtgccc
     3481 aagcctctgc aatgtgctca agagtggagt cctctccagg agagtcagcc caggctatgt
     3541 cccagcctgc agggcagagg atgggggctt ttccccagtg caatgtgacc aggcccaggg
     3601 cagctgctgg tgtgtcatgg acagcggaga agaggtgcct gggacgcgcg tgaccggggg
     3661 ccagcccgcc tgtgagagcc cgcggtgtcc gctgccattc aacgcgtcgg aggtggttgg
     3721 tggaacaatc ctgtgtgaga caatctcggg ccccacaggc tctgccatgc agcagtgcca
     3781 attgctgtgc cgccagggct cctggagcgt gtttccacca gggccattga tatgtagcct
     3841 ggagagcgga cgctgggagt cacagctgcc tcagccccgg gcctgccaac ggccccagct
     3901 gtggcagacc atccagaccc aagggcactt tcagctccag ctcccgccgg gcaagatgtg
     3961 cagtgctgac tacgcggatt tgctgcagac tttccaggtt ttcatattgg atgagctgac
     4021 agcccgcggc ttctgccaga tccaggtgaa gacttttggc accctggttt ccattcctgt
     4081 ctgcaacaac tcctctgtgc aggtgggttg tctgaccagg gagcgtttag gagtgaatgt
     4141 tacatggaaa tcacggcttg aggacatccc agtggcttct cttcctgact tacatgacat
     4201 tgagagagcc ttggtgggca aggatctcct tgggcgcttc acagatctga tccagagtgg
     4261 ctcattccag cttcatctgg actccaagac gttcccagcg gaaaccatcc gcttcctcca
     4321 aggggaccac tttggcacct ctcccaggac atggtttggg tgctcggaag gattctacca
     4381 agtcttgaca agtgaggcca gtcaggacgg actgggatgc gttaagtgtc ctgaaggaag
     4441 ctattcccaa gatgaggaat gcattccttg tcctgttgga ttctaccaag aacaggcagg
     4501 gagcttggcc tgtgtcccat gtcctgtggg cagaacgacc atttctgctg gagctttcag
     4561 ccagactcac tgtgtcactg actgtcagag gaacgaagca ggcctgcaat gtgaccagaa
     4621 tggccagtat cgagccagcc agaaggacag gggcagtggg aaggccttct gtgtggacgg
     4681 cgaggggcgg aggctgccat ggtgggaaac agaggcccct cttgaggact cacagtgttt
     4741 gatgatgcag aagtttgaga aggttccaga atcaaaggtg atcttcgacg ccaatgctcc
     4801 tgtggctgtc agatccaaag ttcctgattc tgagttcccc gtgatgcagt gcttgacaga
     4861 ttgcacagag gacgaggcct gcagcttctt caccgtgtcc acgacggagc cagagatttc
     4921 ctgtgatttc tatgcttgga caagtgacaa tgttgcctgc atgacttctg accagaaacg
     4981 agatgcactg gggaactcaa aggccaccag ctttggaagt cttcgctgcc aggtgaaagt
     5041 gaggagccat ggtcaagatt ctccagctgt gtatttgaaa aagggccaag gatccaccac
     5101 aacacttcag aaacgctttg aacccactgg tttccaaaac atgctttctg gattgtacaa
     5161 ccccattgtg ttctcagcct caggagccaa tctaaccgat gctcacctct tctgtcttct
     5221 tgcatgcgac cgtgatctgt gttgcgatgg cttcgtcctc acacaggttc aaggaggtgc
     5281 catcatctgt gggttgctga gctcacccag tgtcctgctt tgtaatgtca aagactggat
     5341 ggatccctct gaagcctggg ctaatgctac atgtcctggt gtgacatatg accaggagag
     5401 ccaccaggtg atattgcgtc ttggagacca ggagttcatc aagagtctga cacccttaga
     5461 aggaactcaa gacaccttta ccaattttca gcaggtttat ctctggaaag attctgacat
     5521 ggggtctcgg cctgagtcta tgggatgtag aaaagacaca gtgccaaggc cagcatctcc
     5581 aacagaagca ggtttgacaa cagaactttt ctcccctgtg gacctcaacc aggtcattgt
     5641 caatggaaat caatcactat ccagccagaa gcactggctt ttcaagcacc tgttttcagc
     5701 ccagcaggca aacctatggt gcctttctcg ttgtgtgcag gagcactctt tctgtcagct
     5761 cgcagagata acagagagtg catccttgta cttcacctgc accctctacc cagaggcaca
     5821 ggtgtgtgat gacatcatgg agtccaatgc ccagggctgc agactgatcc tgcctcagat
     5881 gccaaaggcc ctgttccgga agaaagttat actggaagat aaagtgaaga acttttacac
     5941 tcgcctgccg ttccaaaaac tgatggggat atccattaga aataaagtgc ccatgtctga
     6001 aaaatctatt tctaatgggt tctttgaatg tgaacgacgg tgcgatgcgg acccatgctg
     6061 cactggcttt ggatttctaa atgtttccca gttaaaagga ggagaggtga catgtctcac
     6121 tctgaacagc ttgggaattc agatgtgcag tgaggagaat ggaggagcct ggcgcatttt
     6181 ggactgtggc tctcctgaca ttgaagtcca cacctatccc ttcggatggt accagaagcc
     6241 cattgctcaa aataatgctc ccagtttttg ccctttggtt gttctgcctt ccctcacaga
     6301 gaaagtgtct ctggactcgt ggcagtccct ggccctctct tcagtggttg ttgatccatc
     6361 cattaggcac tttgatgttg cccatgtcag cactgctgcc accagcaatt tctctgctgt
     6421 ccgagacctc tgtttgtcgg aatgttccca acatgaggcc tgtctcatca ccactctgca
     6481 aacccaacct ggggctgtga gatgtatgtt ctatgctgat actcaaagct gcacacatag
     6541 tctgcagggt cagaactgcc gacttctgct tcgtgaagag gccacccaca tctaccggaa
     6601 gccaggaatc tctctgctca gctatgaggc atctgtacct tctgtgccca tttccaccca
     6661 tggccggctg ctgggcaggt cccaggccat ccaggtgggt acctcatgga agcaagtgga
     6721 ccagttcctt ggagttccat atgctgcccc gcccctggca gagaggcgct tccaggcacc
     6781 agagcccttg aactggacag gctcctggga tgccagcaag ccaagggcca gctgctggca
     6841 gccaggcacc agaacatcca cgtctcctgg agtcagtgaa gattgtttgt atctcaatgt
     6901 gttcatccct cagaatgtgg cccctaacgc gtctgtgctg gtgttcttcc acaacaccat
     6961 ggacagggag gagagtgaag gatggccggc tatcgacggc tccttcttgg ctgctgttgg
     7021 caacctcatc gtggtcactg ccagctaccg agtgggtgtc ttcggcttcc tgagttctgg
     7081 gtccggagag gtgagtggca actgggggct gctggaccag gtggcggctc tgacctgggt
     7141 gcagacccac atccgaggat ttggcgggga ccctcggcgc gtgtccctgg cagcagaccg
     7201 tggcggggct gatgtggcca gcatccacct tctcacggcc agggccacca actcccaact
     7261 tttccggaga gctgtgctga tgggaggctc cgcactctcc ccggccgccg tcatcagcca
     7321 tgagagggct cagcagcagg caattgcttt ggcaaaggag gtcagttgcc ccatgtcatc
     7381 cagccaagaa gtggtgtcct gcctccgcca gaagcctgcc aatgtcctca atgatgccca
     7441 gaccaagctc ctggccgtga gtggcccttt ccactactgg ggtcctgtga tcgatggcca
     7501 cttcctccgt gagcctccag ccagagcact gaagaggtct ttatgggtag aggtcgatct
     7561 gctcattggg agttctcagg acgacgggct catcaacaga gcaaaggctg tgaagcaatt
     7621 tgaggaaagt cgaggccgga ccagtagcaa aacagccttt taccaggcac tgcagaattc
     7681 tctgggtggc gaggactcag atgcccgcgt cgaggctgct gctacatggt attactctct
     7741 ggagcactcc acggatgact atgcctcctt ctcccgggct ctggagaatg ccacccggga
     7801 ctactttatc atctgcccta taatcgacat ggccagtgcc tgggcaaaga gggcccgagg
     7861 aaacgtcttc atgtaccatg ctcctgaaaa ctacggccat ggcagcctgg agctgctggc
     7921 ggatgttcag tttgccttgg ggcttccctt ctacccagcc tacgaggggc agttttctct
     7981 ggaggagaag agcctgtcgc tgaaaatcat gcagtacttt tcccacttca tcagatcagg
     8041 aaatcccaac tacccttatg agttctcacg gaaagtaccc acatttgcaa ccccctggcc
     8101 tgactttgta ccccgtgctg gtggagagaa ctacaaggag ttcagtgagc tgctccccaa
     8161 tcgacagggc ctgaagaaag ccgactgctc cttctggtcc aagtacatct cgtctctgaa
     8221 gacatctgca gatggagcca agggcgggca gtcagcagag agtgaagagg aggagttgac
     8281 ggctggatct gggctaagag aagatctcct aagcctccag gaaccaggct ctaagaccta
     8341 cagcaagtga ccagcccttg agctccccaa aaacctcacc cgaggctgcc cactatggtc
     8401 atctttttct ctaaaatagc cacttacctt caataaagta tctacatgcg gtgaa
//
