LOCUS       NM_006528               2207 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens tissue factor pathway inhibitor 2 (TFPI2), transcript
            variant 1, mRNA.
ACCESSION   NM_006528
VERSION     NM_006528.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Kobayashi,H., Matsubara,S., Yoshimoto,C., Shigetomi,H. and
            Imanaka,S.
  TITLE     Tissue factor pathway inhibitor 2: Current understanding,
            challenges, and future perspectives
  JOURNAL   J Obstet Gynaecol Res 49 (11), 2575-2583 (2023)
   PUBMED   37549932
  REMARK    GeneRIF: Tissue factor pathway inhibitor 2: Current understanding,
            challenges, and future perspectives.
            Review article
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Wang,G., Zhang,G., Zhu,N., Zhu,C., Kang,M., Zuo,G., Niu,Z., Ye,W.,
            Tian,B. and Cai,R.
  TITLE     Integrative analyses of RNA-seq and ChIP-seq Reveal MITF as a
            Target Gene of TFPI-2 in MDA231 Cells
  JOURNAL   Biochem Genet 61 (5), 1745-1757 (2023)
   PUBMED   36787085
  REMARK    GeneRIF: Integrative analyses of RNA-seq and ChIP-seq Reveal MITF
            as a Target Gene of TFPI-2 in MDA231 Cells.
REFERENCE   3  (bases 1 to 2207)
  AUTHORS   Kobayashi,H., Matsubara,S. and Imanaka,S.
  TITLE     The role of tissue factor pathway inhibitor 2 in the coagulation
            and fibrinolysis system
  JOURNAL   J Obstet Gynaecol Res 49 (7), 1677-1683 (2023)
   PUBMED   37186495
  REMARK    GeneRIF: The role of tissue factor pathway inhibitor 2 in the
            coagulation and fibrinolysis system.
            Review article
REFERENCE   4  (bases 1 to 2207)
  AUTHORS   Hube,F., Reverdiau,P., Iochmann,S., Cherpi-Antar,C. and Gruel,Y.
  TITLE     Characterization and functional analysis of TFPI-2 gene promoter in
            a human choriocarcinoma cell line
  JOURNAL   Thromb Res 109 (4), 207-215 (2003)
   PUBMED   12757776
  REMARK    GeneRIF: the minimal TFPI2 promoter is located between -166 and
            -111 from the translation start site; three transcription
            initiation sites and several putative transcription factor binding
            sites were identified, and potential regulatory regions were
            characterized.
REFERENCE   5  (bases 1 to 2207)
  AUTHORS   Kamei,S., Kazama,Y., Kuijper,J.L., Foster,D.C. and Kisiel,W.
  TITLE     Genomic structure and promoter activity of the human tissue factor
            pathway inhibitor-2 gene
  JOURNAL   Biochim Biophys Acta 1517 (3), 430-435 (2001)
   PUBMED   11342222
REFERENCE   6  (bases 1 to 2207)
  AUTHORS   Petersen,L.C., Sprecher,C.A., Foster,D.C., Blumberg,H., Hamamoto,T.
            and Kisiel,W.
  TITLE     Inhibitory properties of a novel human Kunitz-type protease
            inhibitor homologous to tissue factor pathway inhibitor
  JOURNAL   Biochemistry 35 (1), 266-272 (1996)
   PUBMED   8555184
REFERENCE   7  (bases 1 to 2207)
  AUTHORS   Miyagi,Y., Koshikawa,N., Yasumitsu,H., Miyagi,E., Hirahara,F.,
            Aoki,I., Misugi,K., Umeda,M. and Miyazaki,K.
  TITLE     cDNA cloning and mRNA expression of a serine proteinase inhibitor
            secreted by cancer cells: identification as placental protein 5 and
            tissue factor pathway inhibitor-2
  JOURNAL   J Biochem 116 (5), 939-942 (1994)
   PUBMED   7896752
REFERENCE   8  (bases 1 to 2207)
  AUTHORS   Sprecher,C.A., Kisiel,W., Mathewes,S. and Foster,D.C.
  TITLE     Molecular cloning, expression, and partial characterization of a
            second human tissue-factor-pathway inhibitor
  JOURNAL   Proc Natl Acad Sci U S A 91 (8), 3353-3357 (1994)
   PUBMED   8159751
REFERENCE   9  (bases 1 to 2207)
  AUTHORS   Butzow,R., Huhtala,M.L., Bohn,H., Virtanen,I. and Seppala,M.
  TITLE     Purification and characterization of placental protein 5
  JOURNAL   Biochem Biophys Res Commun 150 (1), 483-490 (1988)
   PUBMED   3276312
  REMARK    Erratum:[Biochem Biophys Res Commun 1988 Feb 29;151(1):630-1]
REFERENCE   10 (bases 1 to 2207)
  AUTHORS   Takayama,M., Soma,H., Saito,T., Isaka,K., Kashiwagi,H., Ogawa,T.,
            Suzuki,Y. and Sayama,S.
  TITLE     Circulating levels of placental protein 5 in normal and abnormal
            pregnancies
  JOURNAL   Gynecol Obstet Invest 16 (1), 13-26 (1983)
   PUBMED   6884838
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK129833.1 and AC002076.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006528.3.
            
            Summary: This gene encodes a member of the Kunitz-type serine
            proteinase inhibitor family. The protein can inhibit a variety of
            serine proteases including factor VIIa/tissue factor, factor Xa,
            plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has
            been identified as a tumor suppressor gene in several types of
            cancer. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129833.1, BC005330.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149398, SAMEA2162568
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222543.11/ ENSP00000222543.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2206              AK129833.1         1-2206
            2207-2207           AC002076.2         101861-101861       c
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.3"
     gene            1..2207
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="tissue factor pathway inhibitor 2"
                     /db_xref="GeneID:7980"
                     /db_xref="HGNC:HGNC:11761"
                     /db_xref="MIM:600033"
     exon            1..163
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..783
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; placental protein 5; retinal pigment epithelium
                     cell factor 1"
                     /codon_start=1
                     /product="tissue factor pathway inhibitor 2 isoform 1
                     precursor"
                     /protein_id="NP_006519.1"
                     /db_xref="CCDS:CCDS5632.1"
                     /db_xref="GeneID:7980"
                     /db_xref="HGNC:HGNC:11761"
                     /db_xref="MIM:600033"
                     /translation="MDPARPLGLSILLLFLTEAALGDAAQEPTGNNAEICLLPLDYGP
                     CRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSV
                     DDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKIPSFCYS
                     PKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDCKRACAKALKKKKKM
                     PKLRFASRIRKIRKKQF"
     exon            164..346
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            347..535
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..706
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            707..2207
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1032..1037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: TATAAA"
     polyA_site      1056
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="major polyA site"
     regulatory      1146..1151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: AATAAA"
     regulatory      1163..1168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: AATAAA"
     polyA_site      1173
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
     polyA_site      1187
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
     regulatory      1534..1539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: AGTAAA"
     polyA_site      1553
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
     regulatory      1751..1756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: ATTAAA"
     polyA_site      1768
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
     regulatory      2190..2195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
                     /note="hexamer: AATAAA"
     polyA_site      2207
                     /gene="TFPI2"
                     /gene_synonym="PP5; REF1; TFPI-2"
ORIGIN      
        1 agaaagccgc gcacctcctc ccgccaggcg ctttctcgga cgccttgccc agcgggccgc
       61 ccgaccccct gcaccatgga ccccgctcgc cccctggggc tgtcgattct gctgcttttc
      121 ctgacggagg ctgcactggg cgatgctgct caggagccaa caggaaataa cgcggagatc
      181 tgtctcctgc ccctagacta cggaccctgc cgggccctac ttctccgtta ctactacgac
      241 aggtacacgc agagctgccg ccagttcctg tacgggggct gcgagggcaa cgccaacaat
      301 ttctacacct gggaggcttg cgacgatgct tgctggagga tagaaaaagt tcccaaagtt
      361 tgccggctgc aagtgagtgt ggacgaccag tgtgaggggt ccacagaaaa gtatttcttt
      421 aatctaagtt ccatgacatg tgaaaaattc ttttccggtg ggtgtcaccg gaaccggatt
      481 gagaacaggt ttccagatga agctacttgt atgggcttct gcgcaccaaa gaaaattcca
      541 tcattttgct acagtccaaa agatgaggga ctgtgctctg ccaatgtgac tcgctattat
      601 tttaatccaa gatacagaac ctgtgatgct ttcacctata ctggctgtgg agggaatgac
      661 aataactttg ttagcaggga ggattgcaaa cgtgcatgtg caaaagcttt gaaaaagaaa
      721 aagaagatgc caaagcttcg ctttgccagt agaatccgga aaattcggaa gaagcaattt
      781 taaacattct taatatgtca tcttgtttgt ctttatggct tatttgcctt tatggttgta
      841 tctgaagaat aatatgacag catgaggaaa caaatcattg gtgatttatt caccagtttt
      901 tattaataca agtcactttt taaaaaattt ggattttttt atatataact agctgctatt
      961 caaatgtgag tctaccattt ttaatttatg gttcaactgt ttgtgagact gaattcttgc
     1021 aatgcataag atataaaagc aaatatgact cactcatttc ttggggtcgt attcctgatt
     1081 tcagaagagg atcataactg aaacaacata agacaatata atcatgtgct tttaacatat
     1141 ttgagaataa aaaggactag caaataaaac tcattttgca tttaaaagtt ggattatatt
     1201 ttagtcccaa gaagacaaag tcgcagatta acaacacttt aaaaatatta ctcccatttt
     1261 attgtgttag attgcagtac aatgaatgaa taaggggatt tatttaaaag atcttaactt
     1321 tcttgactga tttaaaaagc taatcaattt attaaagcca gaaatgttct cctctgggaa
     1381 agcaatttct atctatttat ctaccaatcc ccactcacat gtatatgttt gtgtttacat
     1441 agagatagat ggttttaagg gttttcctat tgttaaatta tttcctaaga cattgtgggt
     1501 tggagaccac taaacttata aaaaaccaag gaaagtaaag tttttcaaat gtttatactg
     1561 tttaaggtgt ttatttatat aaaccatgca tgagaaacca tgcataaatt taataattta
     1621 aatttcacca gtaaattatc attgattaaa tatggtcata ccaatgaaat aggttttttc
     1681 tttctctgta ataaaacaat ttttctaaat tcttatgttt tcatagcaag attaacatat
     1741 ttaattacaa attaaatatg caaaatgaca aatgcgttta ccttttgctt gaacaatctt
     1801 tcatttaaaa atttatccca gttattttga caaataggta tatgcctgag aactttgaat
     1861 gatgctgaaa gctagaagca aaccaggtac tcttcagtca gggactggtt gaagatttta
     1921 tggatgagga gttagcaaaa tatctatata tgcatatctt ctgactccca ggatacataa
     1981 gaaacacagg gcctagaaca gtatgaataa acacacatat acacaaacat cacagaagat
     2041 actaaagata ttaaaaatgt ttacctatag aggtttggag agaacagagg ggtggacagt
     2101 tgtggagata atatttgtgt aaatataact gtgatatagt ttttatcttg gaccatgtaa
     2161 atatttgaca taattaataa tcaaaattaa ataaaaaaga aaaagca
//
