LOCUS       NM_006287               3859 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens tissue factor pathway inhibitor (TFPI), transcript
            variant 1, mRNA.
ACCESSION   NM_006287
VERSION     NM_006287.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Sletten,M., Skogstrom,K.B., Lind,S.M., Tinholt,M., Stavik,B.,
            Rayner,S. and Iversen,N.
  TITLE     Elevated TFPI is a prognostic factor in hepatocellular carcinoma:
            Putative role of miR-7-5p and miR-1236-3p
  JOURNAL   Thromb Res 241, 109073 (2024)
   PUBMED   38945092
  REMARK    GeneRIF: Elevated TFPI is a prognostic factor in hepatocellular
            carcinoma: Putative role of miR-7-5p and miR-1236-3p.
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wang,B., Fan,X., Wang,L. and Wei,X.
  TITLE     The RNA-binding protein sorbin and SH3 domain-containing 2 are
            transcriptionally regulated by specificity protein 1 and function
            as tumor suppressors in bladder cancer by stabilizing tissue factor
            pathway inhibitor
  JOURNAL   Mol Carcinog 63 (6), 1174-1187 (2024)
   PUBMED   38501385
  REMARK    GeneRIF: The RNA-binding protein sorbin and SH3 domain-containing 2
            are transcriptionally regulated by specificity protein 1 and
            function as tumor suppressors in bladder cancer by stabilizing
            tissue factor pathway inhibitor.
REFERENCE   3  (bases 1 to 3859)
  AUTHORS   Li,X., Song,X., Mahmood,D.F.D., Sim,M.M.S., Bidarian,S.J. and
            Wood,J.P.
  TITLE     Activated protein C, protein S, and tissue factor pathway inhibitor
            cooperate to inhibit thrombin activation
  JOURNAL   Thromb Res 230, 84-93 (2023)
   PUBMED   37660436
  REMARK    GeneRIF: Activated protein C, protein S, and tissue factor pathway
            inhibitor cooperate to inhibit thrombin activation.
REFERENCE   4  (bases 1 to 3859)
  AUTHORS   Wittig,J., Drekolia,M.K., Kyselova,A., Delgado Lagos,F.,
            Bochenek,M.L., Hu,J., Schafer,K., Fleming,I. and Bibli,S.I.
  TITLE     Endothelial-dependent S-Sulfhydration of tissue factor pathway
            inhibitor regulates blood coagulation
  JOURNAL   Redox Biol 62, 102694 (2023)
   PUBMED   37030150
  REMARK    GeneRIF: Endothelial-dependent S-Sulfhydration of tissue factor
            pathway inhibitor regulates blood coagulation.
REFERENCE   5  (bases 1 to 3859)
  AUTHORS   Mast,A.E.
  TITLE     Tissue Factor Pathway Inhibitor: Multiple Anticoagulant Activities
            for a Single Protein
  JOURNAL   Arterioscler Thromb Vasc Biol 36 (1), 9-14 (2016)
   PUBMED   26603155
  REMARK    Review article
REFERENCE   6  (bases 1 to 3859)
  AUTHORS   Maroney,S.A., Ellery,P.E. and Mast,A.E.
  TITLE     Alternatively spliced isoforms of tissue factor pathway inhibitor
  JOURNAL   Thromb Res 125 Suppl 1, S52-S56 (2010)
   PUBMED   20176395
  REMARK    Review article
            Erratum:[Thromb Res. 2010 Oct;126(4):361]
REFERENCE   7  (bases 1 to 3859)
  AUTHORS   Van der Logt,C.P., Kluck,P.M., Wiegant,J., Landegent,J.E. and
            Reitsma,P.H.
  TITLE     Refined regional assignment of the human tissue factor pathway
            inhibitor (TFPI) gene to chromosome band 2q32 by non-isotopic in
            situ hybridization
  JOURNAL   Hum Genet 89 (5), 577-578 (1992)
   PUBMED   1353057
REFERENCE   8  (bases 1 to 3859)
  AUTHORS   Higuchi,D.A., Wun,T.C., Likert,K.M. and Broze,G.J. Jr.
  TITLE     The effect of leukocyte elastase on tissue factor pathway inhibitor
  JOURNAL   Blood 79 (7), 1712-1719 (1992)
   PUBMED   1558967
REFERENCE   9  (bases 1 to 3859)
  AUTHORS   Girard,T.J., Eddy,R., Wesselschmidt,R.L., MacPhail,L.A.,
            Likert,K.M., Byers,M.G., Shows,T.B. and Broze,G.J. Jr.
  TITLE     Structure of the human lipoprotein-associated coagulation inhibitor
            gene. Intro/exon gene organization and localization of the gene to
            chromosome 2
  JOURNAL   J Biol Chem 266 (8), 5036-5041 (1991)
   PUBMED   2002045
REFERENCE   10 (bases 1 to 3859)
  AUTHORS   van der Logt,C.P., Reitsma,P.H. and Bertina,R.M.
  TITLE     Intron-exon organization of the human gene coding for the
            lipoprotein-associated coagulation inhibitor: the factor Xa
            dependent inhibitor of the extrinsic pathway of coagulation
  JOURNAL   Biochemistry 30 (6), 1571-1577 (1991)
   PUBMED   1993173
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY175080.1, J03225.1,
            AC007319.2 and CA418214.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 24, 2018 this sequence version replaced NM_006287.5.
            
            Summary: This gene encodes a Kunitz-type serine protease inhibitor
            that regulates the tissue factor (TF)-dependent pathway of blood
            coagulation. The coagulation process initiates with the formation
            of a factor VIIa-TF complex, which proteolytically activates
            additional proteases (factors IX and X) and ultimately leads to the
            formation of a fibrin clot. The product of this gene inhibits the
            activated factor X and VIIa-TF proteases in an autoregulatory loop.
            Inhibition of the encoded protein restores hemostasis in animal
            models of hemophilia. This gene encodes multiple protein isoforms
            that differ in their inhibitory activity, specificity and cellular
            localization. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform (a,
            also known as TFPIalpha), which is secreted. Variants 1 and 4-6
            encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.23742.1,
                                           SRR1803616.170461.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000233156.9/ ENSP00000233156.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               HY175080.1         72-183
            113-1237            J03225.1           7-1131
            1238-3153           AC007319.2         117415-119330       c
            3154-3859           CA418214.1         12-717              c
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q32.1"
     gene            1..3859
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /note="tissue factor pathway inhibitor"
                     /db_xref="GeneID:7035"
                     /db_xref="HGNC:HGNC:11760"
                     /db_xref="MIM:152310"
     exon            1..236
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            237..359
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..1153
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; anti-convertin; extrinsic pathway inhibitor;
                     tissue factor pathway inhibitor (lipoprotein-associated
                     coagulation inhibitor)"
                     /codon_start=1
                     /product="tissue factor pathway inhibitor isoform a
                     precursor"
                     /protein_id="NP_006278.1"
                     /db_xref="CCDS:CCDS2294.1"
                     /db_xref="GeneID:7035"
                     /db_xref="HGNC:HGNC:11760"
                     /db_xref="MIM:152310"
                     /translation="MIYTMKKVHALWASVCLLLNLAPAPLNADSEEDEEHTIITDTEL
                     PPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKK
                     MCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLG
                     NMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCL
                     TPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQRIS
                     KGGLIKTKRKRKKQRVKIAYEEIFVKNM"
     exon            360..557
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            558..596
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            597..773
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            774..866
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1046
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..3859
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3836..3841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /note="hexamer: AATATA"
     polyA_site      3859
                     /gene="TFPI"
                     /gene_synonym="EPI; LACI; TFI; TFPI1"
                     /note="major polyA site"
ORIGIN      
        1 agacagcagc gactttaggc tggataatag tcaaattctt acctcgctct ttcactgcta
       61 gtaagatcag attgcgtttc tttcagttac tcttcaatcg ccagtttctt gatctgcttc
      121 taaaagaaga agtagagaag ataaatcctg tcttcaatac ctggaaggaa aaacaaaata
      181 acctcaactc cgttttgaaa aaaacattcc aagaactttc atcagagatt ttacttagat
      241 gatttacaca atgaagaaag tacatgcact ttgggcttct gtatgcctgc tgcttaatct
      301 tgcccctgcc cctcttaatg ctgattctga ggaagatgaa gaacacacaa ttatcacaga
      361 tacggagttg ccaccactga aacttatgca ttcattttgt gcattcaagg cggatgatgg
      421 cccatgtaaa gcaatcatga aaagattttt cttcaatatt ttcactcgac agtgcgaaga
      481 atttatatat gggggatgtg aaggaaatca gaatcgattt gaaagtctgg aagagtgcaa
      541 aaaaatgtgt acaagagata atgcaaacag gattataaag acaacattgc aacaagaaaa
      601 gccagatttc tgctttttgg aagaagatcc tggaatatgt cgaggttata ttaccaggta
      661 tttttataac aatcagacaa aacagtgtga acgtttcaag tatggtggat gcctgggcaa
      721 tatgaacaat tttgagacac tggaagaatg caagaacatt tgtgaagatg gtccgaatgg
      781 tttccaggtg gataattatg gaacccagct caatgctgtg aataactccc tgactccgca
      841 atcaaccaag gttcccagcc tttttgaatt tcacggtccc tcatggtgtc tcactccagc
      901 agacagagga ttgtgtcgtg ccaatgagaa cagattctac tacaattcag tcattgggaa
      961 atgccgccca tttaagtaca gtggatgtgg gggaaatgaa aacaatttta cttccaaaca
     1021 agaatgtctg agggcatgta aaaaaggttt catccaaaga atatcaaaag gaggcctaat
     1081 taaaaccaaa agaaaaagaa agaagcagag agtgaaaata gcatatgaag aaatttttgt
     1141 taaaaatatg tgaatttgtt atagcaatgt aacattaatt ctactaaata ttttatatga
     1201 aatgtttcac tatgattttc tatttttctt ctaaaatgct tttaattaat atgttcatta
     1261 aattttctat gcttattgta cttgttatca acacgtttgt atcagagttg cttttctaat
     1321 cttgttaaat tgcttattct aggtctgtaa tttattaact ggctactggg aaattactta
     1381 ttttctggat ctatctgtat tttcatttaa ctacaaatta tcatactacc ggctacatca
     1441 aatcagtcct ttgattccat ttggtgacca tctgtttgag aatatgatca tgtaaatgat
     1501 tatctccttt atagcctgta accagattaa ggaatacagc tcttaaaaaa tcaagaactt
     1561 cctgagtttc acatataaaa tggtgacaaa cacctgcttc tgataaagtt tatccaattt
     1621 tgtatttata gtattctatt gtaataatag aattctatta tgtgcttccc tccttagaca
     1681 tagtttttct ccttttgtac taaccagtac ttccatctat attgagctaa actgtctttg
     1741 aacagtcaac tctgcatcat gtgcctgcct gatttggatt ggtttcactt ttttttttgt
     1801 ttgaccatag cccattctat cagtcaagat gactgaataa ttgtgtgtta aatacaactc
     1861 atgataagga gaacctttat ggaaacaaga agttttatgg caatcgagag ggaattattt
     1921 atatgtactt cttaatacta tgcttcccaa tattttttct tactaaaaat aacaaaaata
     1981 tttaaacctt ctgaaatcta ttcagttatc attaggattt gcacacaatg caactaataa
     2041 ttcatgcttt aaaaagtagg ttatttctgt tgaagagaaa attatcagca gggaagagtc
     2101 tactagaaaa ttcatgtgct agtcattctt ggggatttaa tattttgctg gagttaattc
     2161 actatatcca gacctgtatc tttcatttat tgctgagcca tgtgcagtct ggcatctcaa
     2221 caacctcaac aacctcaaca acctccaaga catgtcagtt tctgtagtgt tcacctcaaa
     2281 tctgacactg gaattggaac tgatttagaa acaaccattt cattcatttg gtatgactag
     2341 cttcatgact agcttcatga atagcttcat gaatagcttc atgacttctc gctaacaatg
     2401 atagtataat tctgcctgtt cttcaggatt gaaattttga actcaattta gatttcctgt
     2461 tttccctcat ataccacatc taaacagtct ccaaatcttt atgctttttt tttttttttt
     2521 cgaaaaagtt cacccaatcc ctaggctttt tacttttttc tttttctttt cttttttctt
     2581 gttttttttt ttttttcatt ttgttttgtt ttgttatgtt tttttagaca gggtcccact
     2641 ctgtcaccca ggctggagtg cagtggtgca atcacaggac actgaaacct cccacctagc
     2701 tgggactaga ggtgcaggcc accacaccag ctaatttatg taatttttgt agagacgagt
     2761 ttcaccacgt tacctaggct tgtcttgaac acctgggctc aagcaatctt ccagccccag
     2821 cctcccaaag tgctgggatt acaggtataa accacaatgc ccccgttttt actctttact
     2881 gcatccttcc catcagtatt aattcctcag aaatttagta cattcctcca ccatttcaaa
     2941 atgctcccag aactcacact agttggtact taactacaac cttcaacctc accatttaat
     3001 tttgatgtat gtttttggat ttaccaacag tactttcttt catgactact ttgatttagt
     3061 taagaatgct cactgtacac tgaatgttaa ttactcagct aattcacact gatttcctgt
     3121 ttaaaaatat gatcccctcc aggccattca tgtgaagtca gggtacagct gatgccagct
     3181 acagaatcac tatgtttcaa tatggaatat gaaaagtcct aacacataat tgcaaatcat
     3241 tttctactat ggtatacaaa ttttatcatt acaaaatagt atcagctttt ttcacttaag
     3301 gaaaataata catcttcatt caaatattca gtgcataaat aattgcctta aattgagtct
     3361 ttgctgtgga atttgagagt tttggaaatc aaaggccctt gtgaaacatt tcctgttgcc
     3421 atttatttat agatagcaat agcttcaaat gagtgggaaa gtctacttag gtgaagtggt
     3481 aaatagtcac taagattgta taaatagcgc attgatgagt tgcctaattg ttttctccaa
     3541 caataaccac tggtccattg gttcactact tactcaagaa agacatatac tgagatggct
     3601 tgaaatgttt aatgcatgtg aacgttattt ttaccgtgtt ttggaaaaag atcttgatgt
     3661 tattgtcttc ctttattttc cacaattaag tgcatctaac tgtgaattgt atatggaatt
     3721 acattattca gcatatacat atatttacct gctttgttca ttctctgtat tcaaaattaa
     3781 cagatcatgc tgaaaaccac tcaaacgatt atatttttta cattataaga agtaaaatat
     3841 attattctga ataatgaaa
//
