LOCUS       NM_001350162           11476 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens testis expressed 15, meiosis and synapsis associated
            (TEX15), transcript variant 1, mRNA.
ACCESSION   NM_001350162 NM_031271
VERSION     NM_001350162.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Boroujeni,P.B., Sabbaghian,M., Totonchi,M., Sodeifi,N.,
            Sarkardeh,H., Samadian,A., Sadighi-Gilani,M.A. and Gourabi,H.
  TITLE     Expression analysis of genes encoding TEX11, TEX12, TEX14 and TEX15
            in testis tissues of men with non-obstructive azoospermia
  JOURNAL   JBRA Assist Reprod 22 (3), 185-192 (2018)
   PUBMED   29932616
  REMARK    GeneRIF: This result suggests that regular expression of TEX11,
            TEX12, TEX14 and TEX15 is essential for the early stages of
            spermatogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   Wang,X., Jin,H.R., Cui,Y.Q., Chen,J., Sha,Y.W. and Gao,Z.L.
  TITLE     Case study of a patient with cryptozoospermia associated with a
            recessive TEX15 nonsense mutation
  JOURNAL   Asian J Androl 20 (1), 101-102 (2018)
   PUBMED   28303806
  REMARK    GeneRIF: TEX15 could become a clinical marker for detection of
            nonobstructive azoospermia or cryptozoospermia--{CASE REPORT}
REFERENCE   3  (bases 1 to 11476)
  AUTHORS   Lin,X., Chen,Z., Gao,P., Gao,Z., Chen,H., Qi,J., Liu,F., Ye,D.,
            Jiang,H., Na,R., Yu,H., Shi,R., Lu,D., Zheng,S.L., Mo,Z., Sun,Y.,
            Ding,Q. and Xu,J.
  TITLE     TEX15: A DNA repair gene associated with prostate cancer risk in
            Han Chinese
  JOURNAL   Prostate 77 (12), 1271-1278 (2017)
   PUBMED   28730685
  REMARK    GeneRIF: In Han Chinese, the rare variant, Q1631H in DNA repair
            gene, TEX15, is associated with prostate cancer risk.
REFERENCE   4  (bases 1 to 11476)
  AUTHORS   Mantere,T., Tervasmaki,A., Nurmi,A., Rapakko,K., Kauppila,S.,
            Tang,J., Schleutker,J., Kallioniemi,A., Hartikainen,J.M.,
            Mannermaa,A., Nieminen,P., Hanhisalo,R., Lehto,S., Suvanto,M.,
            Grip,M., Jukkola-Vuorinen,A., Tengstrom,M., Auvinen,P., Kvist,A.,
            Borg,A., Blomqvist,C., Aittomaki,K., Greenberg,R.A., Winqvist,R.,
            Nevanlinna,H. and Pylkas,K.
  TITLE     Case-control analysis of truncating mutations in DNA damage
            response genes connects TEX15 and FANCD2 with hereditary breast
            cancer susceptibility
  JOURNAL   Sci Rep 7 (1), 681 (2017)
   PUBMED   28386063
  REMARK    GeneRIF: Our results indicate a potential role in breast cancer
            predisposition for heterozygous truncating mutations in FANCD2 and
            TEX15. Based on our results, FANCD2 c.2715 + 1G > A might act as a
            moderate breast cancer risk allele, adding FANCD2 to the list of
            shared genes between FA and breast cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11476)
  AUTHORS   Colombo,R., Pontoglio,A. and Bini,M.
  TITLE     Two Novel TEX15 Mutations in a Family with Nonobstructive
            Azoospermia
  JOURNAL   Gynecol Obstet Invest 82 (3), 283-286 (2017)
   PUBMED   28355598
REFERENCE   6  (bases 1 to 11476)
  AUTHORS   Okutman,O., Muller,J., Baert,Y., Serdarogullari,M., Gultomruk,M.,
            Piton,A., Rombaut,C., Benkhalifa,M., Teletin,M., Skory,V.,
            Bakircioglu,E., Goossens,E., Bahceci,M. and Viville,S.
  TITLE     Exome sequencing reveals a nonsense mutation in TEX15 causing
            spermatogenic failure in a Turkish family
  JOURNAL   Hum Mol Genet 24 (19), 5581-5588 (2015)
   PUBMED   26199321
  REMARK    GeneRIF: Data identified nonsense mutation in TEX15 gene
            co-segregating with the infertility phenotype, as the cause of
            spermatogenic defects.
REFERENCE   7  (bases 1 to 11476)
  AUTHORS   Ruan,J., He,X.J., Du,W.D., Chen,G., Zhou,Y., Xu,S., Zuo,X.B.,
            Fang,L.B., Cao,Y.X. and Zhang,X.J.
  TITLE     Genetic variants in TEX15 gene conferred susceptibility to
            spermatogenic failure in the Chinese Han population
  JOURNAL   Reprod Sci 19 (11), 1190-1196 (2012)
   PUBMED   22581801
  REMARK    GeneRIF: the polymorphisms rs323346 and rs323347 of the TEX15 gene
            could be considered the genetic risk factors for spermatogenic
            failure in the Chinese Han population
REFERENCE   8  (bases 1 to 11476)
  AUTHORS   Aston,K.I., Krausz,C., Laface,I., Ruiz-Castane,E. and Carrell,D.T.
  TITLE     Evaluation of 172 candidate polymorphisms for association with
            oligozoospermia or azoospermia in a large cohort of men of European
            descent
  JOURNAL   Hum Reprod 25 (6), 1383-1397 (2010)
   PUBMED   20378615
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 11476)
  AUTHORS   Yang,F., Eckardt,S., Leu,N.A., McLaughlin,K.J. and Wang,P.J.
  TITLE     Mouse TEX15 is essential for DNA double-strand break repair and
            chromosomal synapsis during male meiosis
  JOURNAL   J Cell Biol 180 (4), 673-679 (2008)
   PUBMED   18283110
REFERENCE   10 (bases 1 to 11476)
  AUTHORS   Loriot,A., Boon,T. and De Smet,C.
  TITLE     Five new human cancer-germline genes identified among 12 genes
            expressed in spermatogonia
  JOURNAL   Int J Cancer 105 (3), 371-376 (2003)
   PUBMED   12704671
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090281.8, BC141841.1,
            AF285605.1 and AL133653.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001350162.1.
            
            Summary: This gene encodes a protein that is required for DNA
            double-strand break repair, chromosome synapsis, and meiotic
            recombination in spermatocytes. Male mice with a knockout of the
            orthologous gene are viable but sterile. Loss-of-function mutations
            in the orthologous mouse gene cause early meiotic arrest in
            spermatocytes, before the mid-pachytene stage. Naturally occurring
            mutations in this gene are associated with nonobstructive
            azoospermia. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2148874
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000643185.2/ ENSP00000493555.1
            RefSeq Select criteria    :: based on single protein-coding
                                         transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               AC090281.8         81532-81661         c
            131-206             AC090281.8         67395-67470         c
            207-587             BC141841.1         72-452
            588-646             AC090281.8         36027-36085         c
            647-1227            BC141841.1         511-1091
            1228-5680           AC090281.8         13355-17807         c
            5681-9734           AF285605.1         4317-8370
            9735-11476          AL133653.1         791-2532
FEATURES             Location/Qualifiers
     source          1..11476
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p12"
     gene            1..11476
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /note="testis expressed 15, meiosis and synapsis
                     associated"
                     /db_xref="GeneID:56154"
                     /db_xref="HGNC:HGNC:11738"
                     /db_xref="MIM:605795"
     exon            1..130
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            131..206
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            207..351
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..9734
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /note="cancer/testis antigen 42; testis-expressed sequence
                     15 protein"
                     /codon_start=1
                     /product="testis-expressed protein 15"
                     /protein_id="NP_001337091.1"
                     /db_xref="CCDS:CCDS87593.1"
                     /db_xref="GeneID:56154"
                     /db_xref="HGNC:HGNC:11738"
                     /db_xref="MIM:605795"
                     /translation="MEMKETAKQDTLWQMSSTSKPVLNTREVNPLKKFTIPKIRRTAE
                     KVYLSPCYTNSREYSFIHDTLNQCRLDVNCDLQSLWQFGDTKLVHNEELEKNFTAKRS
                     EMRESGRHCRELEEQFCFLALPQSDVAQIYQNGISTRASTLKILGNPLLGIYMFRHVD
                     IALNYSHSQSITVESILIFKVLFGKVKKIQPSVDKNKVSLDPSPNFDCHMSRNAPSLK
                     DTIELQAYSSAVYFYEYSVLSKPVDKPRQCLPYAVVTVKFLGSKVDNGRLMTSLRFLS
                     TGFPKRAERTCSLNNCTVAKRFGKGKDATVTFVHFKKPVDPFVQENCLCNALNSEINP
                     FISNISNSYGNVQNGNISIPETYSGQTEHSLAEIRDTSQVLAHDSDISLMPSDAKDSV
                     NGDLLLNWTSLKNILSGLNASFPLHNNTGSSTVTTSKSIKDPRLMRREESMGEQSSTA
                     GLNEVLQFEKSSDNVNSEIKSTPSNSASSSEVVPGDCAVLTNGLDTPCFKTSVNDSQS
                     WAHNMGSEDYDCIPPNKVTMAGQCKDQGNFSFPISVSNVVSEVENQNHSEEKAQRAQQ
                     ESGNAYTKEYSSHIFQDSQSSDLKTIYQTGCQTSTVFPLKKKVSIDEYLQNTGKMKNF
                     ADLEDSSKHEEKQTSWKEIDNDFTNETKISPIDNYIVLHQEYKESESHNSFGKSCDKI
                     LITQELEITKSSTSTIKDKDELDHLALEWQITPSFESLSQKHPQHSVEYEGNIHTSLA
                     IAQKLMELKLGKINQNYASIITEAFPKPKDIPQAKEMFIDTVISSYNIETAHDSSNCS
                     ITREHICVHRKNENEPVSLENIQRDYKETAYVEDRGQDHNLFCNSQLSNDIWLNVNFK
                     KQTDRENQNEAKENSASCVENNIENIYGDKKQDSHTNENFSNIDEKEDKNYHNIEILS
                     SEEFSTKFNLICREDNAVSAATALLESEEDTISAVKQKDTENTGRSVEHLASTTFPKT
                     ASSSVCVASNAAIQIASATMPALSLNNDDHQIYQFKETCSSESPDFGLLVKHRVSDCE
                     IDTDKNKSQESFHQSINENLVLQSIELESEIEIELEDCDDAFIFQQDTHSHENMLCEE
                     FVTSYKALKSRISWEGLLALDNGEMEVLESTTGRENSDQHYSKESNYFYSSTQNNETE
                     LTSPILLPDLQIKITNIFRPGFSPTADSLALKDSFCTHVTEATKPEINKEDGEILGFD
                     IYSQPFGENADYPCEDKVDNIRQESGPVSNSEISLSFDLSRNTDVNHTSENQNSESLF
                     TEPSNVTTIDDGSRCFFTKSKTDYNDTKNKKEVESRISKRKLHISSRDQNIPHKDLRR
                     HKIYGRKRRLTSQDSSECFSSLSQGRIKTFSQSEKHIKSVLNILSDEASLCKSKCLSR
                     KLDKAVVHLKKAHRRVHTSLQLITKVGEERKGPLPKSYAIICNNFWESCDLQGYSSVS
                     QRKYYSTKHFSSKRKYDKRRKKRAPKADISKSLTHVSKHKSYKTSGEKKCLSRKSMAS
                     SVSKSHPTTSHMGEFCNQEHPESQLPVSSTSQSTSQSVYYNSSVSNPSLSEEHQPFSG
                     KTAYLFSPDHSDEKLIEKENQIDTAFLSSTSKYEKLEKHSANHNVKDATKENSCDANE
                     VINESNSVSLSCIKENINSSTGNDCDATCIGHTKAKTDVLISVLDSNVKHFLNDLYQQ
                     GNLILSDCKRNLEVKWTDPIERPKQNIITGNFLMGPLNLTLIASKKYSIPQLSAAAVT
                     DSEGESSKSYLDKQRILTVDSFAASSTVPHCEQSCREKELLKTEQCSSGNCLHTDGNE
                     TNVTENYELDVASGTEEDKSYGENIVELSSSDSSLLLKDNVKGSSSETCIVKKDTEDR
                     ITWKVKQAEKAKDSVYKRSMTEGSTVNTEYKNQKNQISEESCLNEKIITTNLIDSHLS
                     TKNTTTESVPLKNTVSNPLNKREKKGEIKVSKDSQSDLTLHSEIAYISKPGILGVNHT
                     PILPAHSETCKVPTLLKKPASYVSDFKEKHCSANHTALIANLSQILQRADEASSLQIL
                     QEETKVCLNILPLFVEAFERKQECSVEQILISRELLVDQNLWNNCKHTLKPCAVDTLV
                     ELQMMMETIQFIENKKRHLEGEPTLRSLLWYDETLYAELLGKPRGFQQQSNFYPGFQG
                     RLKYNAFCELQTYHDQLVELLEETKREKNSYYVFLKYKRQVNECEAIMEHCSDCFDFS
                     LSVPFTCGVNFGDSLEDLEILRKSTLKLINVCGDSPKVHSYPGKQDHLWIIIEMISSK
                     VNFIKNNEAVRVKISLYGLEHIFFDAAKNLVWKERTQSFSKKYSQKKDEERLLRVNKC
                     AFSKLQKIYDTLSKDLNNEPISPIGLEEDTIIASRKSDHPINEATISIENSKFNSNLL
                     AHPDICCISEILDQAEFADLKKLQDLTLRCTDHLEILKKYFQMLQDNNMDNIFITEEN
                     VLDVVINHSHEAIILKPEAIEMYIEIVMVSETIHFLKNSIAKKLDKQRFRGMLWFDLS
                     LLPELVQCQEKMASFSFLKDNSTDVCLWKVIETAVSELKKDLDIICKYNEAVNCSYAI
                     HLLSRELQELSEIKKLLKKSKYFISTYIDFVPYIASINYGSTVTELEYNYNQFSTLLK
                     NVMSAPRKDLGKMAHIRKVMKTIEHMKMICTKNAELTISFFLCQMLYNRRKILQLKRK
                     EKMNIHIVKPGENNNKFSISTMLPPVSECINKNISNSSKKRPSTVDKCEDSQEQQQDT
                     TVSSCKKLKVDMKDVTKINREKATFKHPRTTGSHPKSENKIVPSSCDSLKRNHLTPKK
                     VEMQRSLPGSLLPLENPKDTCASKSESKIDLTVSSDHFSGQQENLNSMKKRNVNFSAA
                     ETKSDKKDCAAFAICDQKSVHGTFSPDHGTLLQKFLKNSPDPTQKSCLSDINPETDVS
                     LVPDASVLSKPIFCFVKDVHPDLEMNDTVFELQDNDIVNSSIKNSSCMTSPEPICIQN
                     KIPTLQINKLQPTETESEDKYMKDTLNPNTVHTFGASGHITLNVNQGAEYSLSEQQND
                     KNSKVLMQNAATYWNELPQSACNPTYNSSEHLFGTSYPYSAWCVYQYSNSNGNAITQT
                     YQGITSYEVQPSPSGLLTTVASTAQGTHSNLLYSQYFTYFAGEPQANGFVPVNGYFQS
                     QIPASNFRQPIFSQYASHQPLPQATYPYLPNRFVPPEVPWVYAPWHQESFHPGH"
     exon            352..517
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            518..755
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            756..902
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1065
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..8378
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            8379..8437
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            8438..9696
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     exon            9697..11476
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11449..11454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /note="hexamer: ATTAAA"
     polyA_site      11476
                     /gene="TEX15"
                     /gene_synonym="CT42; SPGF25"
                     /note="major polyA site"
ORIGIN      
        1 gctcactgtg cgcacctcgc tggggggtgc tgctgtgttc ctgaggcttc cagggaaggc
       61 agtgggcgaa ggtctgtacc gtgctaagat tgagtctgag gaactgactg agcaaagaag
      121 acagctcaag atgttccctt atcagttttc atgtatggac tgagtactga ttataactgt
      181 gtaatgctgg atgccattga agagtggcca acaaaatgga aatgaaagaa actgctaaac
      241 aggatacact gtggcaaatg agctcaacta gcaaacccgt gttgaatact cgtgaagtca
      301 atcctttgaa gaaattcacc attcctaaga tcaggaggac agctgagaaa gtttacttgt
      361 caccttgtta caccaatagt agagagtata gttttataca tgatactctt aaccagtgca
      421 ggcttgatgt aaactgtgat ctgcagtcgt tatggcagtt tggggataca aaactggttc
      481 acaatgagga actggaaaaa aattttactg ctaagaggtc agagatgcgt gaaagtggaa
      541 gacattgcag ggaacttgaa gaacagttct gctttttagc acttccccag agtgatgtag
      601 cccagatata tcagaatgga ataagtacca gagcatctac attgaagata ctaggaaatc
      661 ctcttcttgg aatttatatg tttagacatg ttgatattgc cttgaattat tctcatagtc
      721 aaagcattac tgtagaaagt attttaattt ttaaggttct ctttggaaaa gtgaagaaaa
      781 tccaaccttc tgtggataaa aataaagttt ctttggatcc ttctcctaac tttgattgcc
      841 atatgtcaag aaatgcacct tctttgaaag ataccattga actgcaagcc tacagttcag
      901 cagtgtactt ctatgaatac agtgtccttt caaagcctgt agataaacct aggcaatgtc
      961 ttccatatgc tgtagtaaca gtaaaatttc ttggttcaaa agtagataat ggacgcctta
     1021 tgacatcttt gagattcctc tcaacaggat ttcctaagag agctgaaagg acatgctctc
     1081 tgaataactg tacagtggcc aaaagatttg gaaaaggaaa agatgctact gtcacctttg
     1141 tgcatttcaa gaaacctgta gatccatttg ttcaagaaaa ctgtttatgc aatgcactaa
     1201 attcagagat aaatcctttc atctcaaata tttctaactc ctatggaaat gtacaaaatg
     1261 gaaacatttc tatacctgaa acatacagtg gacagacaga gcacagttta gcagaaatta
     1321 gagacacttc tcaagtactt gcacatgatt cagacatttc acttatgccc agtgatgcca
     1381 aagacagtgt taatggtgac cttttgttaa attggacaag tcttaaaaat attttaagtg
     1441 gtcttaatgc ttcttttcct cttcacaaca atactggctc aagcacagtc actacttcaa
     1501 aatccatcaa agacccaaga ctgatgagga gagaagaaag tatgggagaa cagagtagta
     1561 ctgcaggctt aaatgaggtt ttgcaatttg agaagagttc agataatgtt aattcagaaa
     1621 taaaatcgac accatctaat tctgcctcct cctcagaagt tgtccctggt gattgtgctg
     1681 ttcttactaa tggtttggat accccttgct ttaaaacttc tgttaatgat tcacaatctt
     1741 gggctcacaa catgggctct gaggactatg actgtatacc tcccaataaa gttaccatgg
     1801 cagggcaatg taaggaccaa ggtaattttt ccttcccaat ttctgtgtca aatgtagtgt
     1861 cagaggttga gaaccaaaac cacagtgagg agaaggctca gagagcccaa caggagtccg
     1921 gtaatgctta tacaaaagag tacagtagtc acatttttca ggactcgcag tcttctgatt
     1981 taaaaacaat ttatcagact ggttgccaaa cgtctacagt ttttccactc aaaaagaaag
     2041 taagcattga tgaatacctt caaaatactg gaaagatgaa aaacttcgct gacctggaag
     2101 acagttccaa acatgaagaa aagcaaactt catggaaaga aattgataat gatttcacta
     2161 atgaaacaaa aatcagtcca atagataatt acattgtttt gcaccaagaa tacaaagaga
     2221 gtgagagtca taattctttt gggaaaagct gtgataaaat attaattact caagagttag
     2281 aaataacaaa atcttctaca tctaccataa aggataagga tgaactagat catctagcat
     2341 tggaatggca aattactcca agttttgaga gcctgtcaca aaagcatcct cagcactctg
     2401 tggagtatga gggtaacatt catacaagtt tagccattgc tcaaaagcta atggaactga
     2461 aattggggaa aataaatcaa aattatgcta gcattataac tgaagctttc ccgaaaccaa
     2521 aagacatacc ccaggccaaa gaaatgttca ttgatacagt tatttcatct tataacatag
     2581 aaacagctca tgacagttca aattgcagca taactagaga acatatatgt gtccatagga
     2641 aaaatgaaaa tgaaccagtg tcattagaga acattcagag agactataaa gaaactgctt
     2701 atgttgaaga taggggtcag gatcacaatc tgttctgtaa ttcacagtta agcaatgata
     2761 tatggctgaa tgttaatttc aaaaaacaaa cagatagaga aaaccaaaat gaggctaaag
     2821 agaatagtgc ttcatgtgta gaaaacaaca tagagaacat atatggagac aaaaagcagg
     2881 attctcatac aaacgaaaat ttcagcaata tagatgaaaa ggaggacaaa aattaccaca
     2941 atatagaaat tttgagttct gaagaatttt ctactaaatt taacttgatt tgcagagaag
     3001 ataatgcagt gtcagcagca actgcattat tagagagtga agaagatacc attagtgccg
     3061 tgaaacaaaa agatactgaa aatactggaa gaagtgtaga gcatttggct tccacgacat
     3121 ttcccaaaac tgcaagttct tcagtgtgtg tagcctcaaa tgctgcaata cagatagcta
     3181 gtgctactat gcctgcatta agcctaaata atgacgatca ccagatatac cagtttaaag
     3241 aaacttgttc ttctgaaagt ccagattttg gtttgttagt aaaacatagg gtttctgatt
     3301 gtgaaattga tacggataaa aataaatcac aagaatcatt tcatcaatca ataaatgaga
     3361 acttagttct tcagagcatt gaattggaaa gtgaaattga aatagaatta gaagattgtg
     3421 atgatgcttt tatatttcaa caagatacac atagccatga aaacatgctt tgtgaagaat
     3481 ttgtgacctc atataaggct ctgaagtctc gtatcagttg ggaaggtctg ttagcacttg
     3541 ataacgggga gatggaagtt ttggaaagca ccacaggaag ggagaatagt gatcagcatt
     3601 attctaagga aagtaactat ttttattcct ctacacaaaa caatgaaaca gaacttacca
     3661 gcccaatttt acttccagat ctacaaatta aaattactaa tatatttagg ccaggattca
     3721 gcccgacagc tgactccctt gcattgaaag atagtttttg cacacatgta actgaagcca
     3781 caaaaccgga aataaataag gaagatggag aaattctagg atttgacatt tattcccagc
     3841 cttttggtga aaatgcagat tatccatgtg aagataaagt tgataatata aggcaagaat
     3901 cagggccagt gagtaactct gaaatctccc tttcttttga cttgagtcgt aatacagatg
     3961 tgaatcatac gtctgaaaat cagaacagtg aatctttgtt tactgaacct tctaatgtca
     4021 caacaataga tgatggaagc agatgtttct ttacaaaatc aaaaactgac tataatgata
     4081 ccaaaaataa aaaggaggta gaatcaagaa ttagcaaaag gaagctacat atatcttcca
     4141 gggatcagaa cataccacat aaagatttaa gacgacataa aatttatggg agaaagagga
     4201 ggctaaccag tcaagactca tctgagtgtt tctcttcatt atcccaagga cgaattaaaa
     4261 cattttcaca gtcagaaaag cacattaaga gtgtcctaaa tatcctaagt gatgaagcat
     4321 ctttatgtaa aagcaaatgt ctttccagaa aactagacaa agcagttgtt cacttaaaaa
     4381 aagctcatag aagagttcac acatctttgc agcttataac taaagtagga gaagaaagaa
     4441 agggcccatt accaaaatca tatgcaataa tatgcaataa tttctgggaa agttgtgacc
     4501 ttcaaggtta tagttctgtg tctcaaagaa aatattattc tactaagcat ttttcgtcaa
     4561 aaagaaaata tgacaaacgg agaaagaaaa gagctccaaa agctgatatt tctaaatcat
     4621 taacccatgt gtcaaagcac aagtcttata aaacaagtgg agagaaaaaa tgcctttcta
     4681 ggaaaagtat ggctagcagt gtctcaaaaa gtcaccccac caccagtcac atgggagaat
     4741 tttgtaatca agaacatcct gaatcacagt tgcctgtatc ctccacatcc caaagtacaa
     4801 gtcagtcagt ttattataat agcagtgtaa gcaatccaag tttatcagaa gaacatcagc
     4861 ccttttctgg aaaaactgca tatctgtttt ccccagacca ctcagatgag aaactaatag
     4921 aaaaagaaaa tcaaattgat acagcatttt tatctagcac tagtaaatat gaaaagcttg
     4981 aaaaacattc agcaaatcat aatgttaaag atgcaactaa agaaaacagt tgtgacgcta
     5041 atgaagtaat aaatgaaagt aattctgtat ctttaagttg cataaaagaa aacataaatt
     5101 ctagtacagg caacgattgt gatgcaactt gcataggtca cacaaaggcg aaaactgacg
     5161 tacttatatc agtcttagat tcaaatgtga agcacttttt aaatgatctc taccaacaag
     5221 gtaaccttat tttatctgat tgtaaaagaa acctggaagt aaagtggaca gatcctattg
     5281 agagacccaa acaaaacatt attacaggaa acttccttat gggcccatta aacctaactt
     5341 tgatagcaag taaaaagtac agtattcctc agttatcagc cgctgcagtg acagatagtg
     5401 agggagaatc ttcaaaatct tacttggata agcagagaat tcttactgta gattcttttg
     5461 cagcatccag tactgtacca cactgtgagc agagctgtag agaaaaagag cttctaaaga
     5521 cagaacagtg ctcttcaggt aattgcctcc atacagatgg gaatgaaaca aatgtcactg
     5581 agaattatga gttggatgta gcatcaggaa ctgaagaaga taaaagttat ggggaaaata
     5641 tagtggaatt atcttccagt gatagttctc tgcttttaaa agataatgta aaaggctcct
     5701 cttcagaaac atgtattgtg aagaaagaca ctgaggacag aataacgtgg aaagttaaac
     5761 aagcggaaaa agcaaaagat tctgtttaca aaagaagcat gactgaagga tcaactgtta
     5821 atactgagta caaaaatcaa aagaatcaga tctcagaaga atcctgctta aatgagaaaa
     5881 ttattacaac taacttgatt gattcccatc tgagcactaa aaatactacc actgagtcag
     5941 tccctttgaa gaacacagtt tctaatccgc ttaacaaaag agagaagaag ggggaaatta
     6001 aagttagtaa agactcgcag tctgacttga cattacattc agaaatagcc tatatttcca
     6061 aaccaggaat tctaggagtt aatcatacgc ctattttacc tgcccactct gaaacctgta
     6121 aagtccctac tcttctgaag aaacctgcgt catacgtgag tgattttaaa gaaaaacatt
     6181 gctcagctaa tcatacggcc cttatagcta atctatctca aattttgcag agggcagatg
     6241 aagcatcatc tttgcagatt ctacaggaag aaactaaggt ttgtctaaat attctccctt
     6301 tatttgtgga agcttttgaa agaaagcaag aatgttcagt tgaacaaatc ctgatttcaa
     6361 gagaactgtt ggtagaccaa aacctgtgga ataattgcaa acacacatta aaaccatgtg
     6421 ctgttgacac tttggtagaa cttcaaatga tgatggaaac aattcaattc attgaaaaca
     6481 aaaaaaggca cttagaaggt gaaccaacat tgcgaagctt gctttggtat gatgaaacac
     6541 tgtatgctga gcttcttgga aaaccacgtg gatttcaaca gcagtctaat ttctatcctg
     6601 gtttccaagg aagattaaaa tataatgcat tctgtgagtt acagacttac catgatcaat
     6661 tagttgaatt gcttgaagaa acaaaaaggg aaaagaattc atactatgta ttcttaaagt
     6721 acaaacgaca ggttaatgaa tgtgaagcca taatggagca ttgttccgat tgctttgatt
     6781 tttctctttc tgttccattt acctgtggag ttaactttgg agatagttta gaagacctgg
     6841 aaatcttaag aaaaagtact ttaaagttga tcaatgtatg tggggactct cctaaagttc
     6901 attcgtatcc aggaaaacag gaccatctgt ggattatcat agaaatgatc tcctcaaagg
     6961 ttaattttat taagaacaac gaggcagtac gtgttaaaat atctctttat ggtctggaac
     7021 atatcttttt tgatgctgca aaaaatcttg tttggaaaga gagaacacaa tccttcagca
     7081 aaaaatactc acaaaagaag gacgaagaaa ggctactcag agtgaataaa tgtgcctttt
     7141 ctaagttgca gaagatatat gatactttgt ctaaagattt aaacaatgaa ccaatttccc
     7201 ctattgggct tgaggaggat actataattg cttccagaaa gtcagatcat ccaataaacg
     7261 aagcaacaat tagcatagaa aattctaaat ttaacagtaa tttgcttgca cacccagata
     7321 tttgttgtat tagtgagata ttggatcagg ctgaatttgc agaccttaaa aaattacagg
     7381 atctcacctt gagatgtaca gatcacttag aaattttaaa aaaatacttt cagatgctac
     7441 aagataataa catggataat atttttatca cagaagaaaa tgttttagac gtggtgataa
     7501 accacagcca tgaggctatc attttaaagc ctgaagctat tgaaatgtat attgaaatcg
     7561 tcatggtctc agaaacaatt cactttctta aaaactcaat agcaaagaaa ctagacaaac
     7621 agaggtttcg aggtatgctt tggtttgatt tgtcacttct tcctgagctg gttcagtgcc
     7681 aagaaaaaat ggcttctttt tcatttctta aagataactc aacagatgtt tgcctttgga
     7741 aagtgataga gactgctgtt tccgaactta agaaagatct ggatattatc tgcaaatata
     7801 atgaagctgt taattgctca tatgctattc atttgctctc aagagaactt caagaacttt
     7861 cagaaataaa aaagcttctg aagaagtcca agtattttat ttccacatat attgactttg
     7921 tgccatatat agcatccata aattatggaa gcactgtgac agagttagaa tacaactaca
     7981 atcaattttc tacactgctg aagaatgtaa tgtctgcccc taggaaagat ttaggaaaaa
     8041 tggcccacat taggaaagtc atgaaaacga ttgaacatat gaagatgata tgtactaaaa
     8101 atgctgaact aaccatttcc tttttcctat gccaaatgct gtataacaga aggaagattt
     8161 tacagctgaa gagaaaagaa aaaatgaata ttcatattgt aaaacctggg gaaaataaca
     8221 ataaatttag tatttctacg atgttgcccc cagtatcaga gtgcataaac aaaaacatct
     8281 caaattcctc taaaaaacga ccgagcactg tagacaaatg tgaagactct caggaacaac
     8341 agcaagatac tactgtttcc agttgtaaaa agctaaaggt agacatgaaa gatgtcacaa
     8401 aaatcaacag agaaaaggca acattcaagc atccaaggac tacaggatct catcccaaaa
     8461 gcgaaaacaa aatagtacca agttcatgtg acagtctgaa aagaaatcat ttaacgccaa
     8521 aaaaggttga aatgcaaaga tcactacctg gctcactttt acccttagag aacccaaaag
     8581 acacttgcgc atcaaagtcg gaaagcaaaa tagacttaac tgtttcatct gatcacttca
     8641 gtggacaaca ggaaaattta aatagcatga agaaaagaaa tgtgaacttc agtgctgctg
     8701 aaacaaaaag tgataagaaa gattgtgctg cttttgcaat ttgtgaccaa aaaagtgtac
     8761 atggcacatt ttcaccagac catgggacgc ttttgcagaa atttcttaaa aattccccag
     8821 atcccaccca aaaatcctgc ctttctgata taaacccaga aactgatgtt tctcttgtgc
     8881 ctgatgcgtc ggtgctctca aagccaattt tctgttttgt gaaagatgtc catcctgatc
     8941 tagaaatgaa tgacacagtc tttgaacttc aagataatga tatagtaaat tcatctatta
     9001 aaaattcctc atgcatgact tctccagaac ccatctgtat ccagaacaaa attcctactc
     9061 tgcagataaa caaactacag cctacagaaa ctgagtcaga ggacaaatac atgaaggata
     9121 cattgaatcc caatactgtg catacttttg gagcatctgg gcatataacc cttaatgtga
     9181 atcaaggagc agagtactct ctttctgaac aacagaatga caaaaattca aaagtcctaa
     9241 tgcagaatgc tgccacatat tggaatgaac ttccacagtc tgcatgtaac ccaacatata
     9301 attcttctga gcatttattt ggaacttcat atccatactc tgcttggtgt gtttatcagt
     9361 acagcaacag caatggcaat gccattaccc agacatacca agggataaca tcatatgaag
     9421 tacagccatc tccttctggg ctgttgacca cagttgcaag tactgcccag ggcacacatt
     9481 ctaatcttct gtactctcaa tattttactt attttgcggg ggagccacaa gcaaatggct
     9541 ttgtgccagt gaatgggtat tttcaatctc aaatacctgc ttctaatttt cggcagccaa
     9601 ttttttcaca atatgcttct catcagccat taccacaagc tacataccct taccttccta
     9661 atcgatttgt gcctccagaa gttccttggg tttatgctcc atggcaccaa gaatcctttc
     9721 atccaggaca ctgaaaatag tcttctgtct attgaaataa gacataacaa gtttttccaa
     9781 atatttttta cttatatatt ttttaacatt tagcgatttt taatgtttgt aaatgtggta
     9841 ggaaatataa atcatatggt cctttgtaaa atatacaaat aatctaacac atatcatata
     9901 ggacatcaat ttctaacatc tgatatacca gaattatttc catgcaatta taaggggagg
     9961 gtaatatgat caaagttaag caatagtggt aaaatcttta aaactagatt cttgcttctg
    10021 atatgtttca atacattgtt gatggcattg tgccatttga gctgaaagca ggattttttt
    10081 tcattagtca tcaaaccaag aattttataa ttcttgacac tttattatat aatttacaat
    10141 tctatgccag atatggcaaa tttcttcaat ttcagtattt tatagtttct acttgtaaaa
    10201 tatttttaat aagaaaaatt ggtttgcata atctcagtgt ttccttgagt agagatgttt
    10261 taaatgttta tgttggaagc aattgtagtt aattatattg gcaatgaagc actttcaatg
    10321 cctttttcta caagagccat ttccagaaga ttattttgtt tttggatttc acaatttaaa
    10381 tagttatttt aatgattttc tagcaaataa tagagaataa tgtttatgct tatgcagtta
    10441 gtctttatta cagtaattta aacaatgtgg cacaaacata gttttttcat gctcttgtca
    10501 tagacttttc tttttaacct tctgcaaaag taaaaactct cttagtaata atcatcatag
    10561 ggcaacagga tatagtatgt aactgcagtc ttgagctgtc tcttttcatg tattaccttt
    10621 cttgctcaac agctattcct gttttctgta agatcctagt cagacagagc cctcacaact
    10681 ggatagacct ttctcaaggg ttagccctaa aatgtgacaa gagaattggt ttggctgaat
    10741 cacaacaaat aactagcaaa gcttcagtgc ttttgcaaac tttttatgta agcaaacttt
    10801 ttgtgttatc tggcacaggc ctttgaaaag actataattc tactttgttt aatgtagagt
    10861 aactaatttt tcaatttaga aagacagtga gaatccccag gataagcctc tttatactca
    10921 cttaaaccaa ctgggatgga attcagaata cctaagctgc ttctttaaca tagagatctt
    10981 taaaaaggga aggtcaagca aagaactgta accgaaagaa gtaagagatt tgaaaagacg
    11041 atagagttaa aacaatattt aaaaaacatt tctatttggt ccatgcagtt ttatttgaaa
    11101 atagtctgtc aaaatcagaa ctcctgaaca actttgaaag cagtaatatt aactggtatt
    11161 caaaaatgaa taacagccaa aaatgaataa cagccatttg atttatgttt ccctcagtca
    11221 tttggtttat gtttggttat ggcaagatgt tacagcattt atagttaata ctactactaa
    11281 tatgtaaacc aaaagttaaa tattctagtt aaggattgtt tctttaaaca actttcagat
    11341 tctgttgaca ttgtctttgt ggtattttta tttcatgggt tttaatttct cttaaagact
    11401 tacctaaata ttcagttaca tttttaaaaa atctgtacgt gacagaatat taaaacatgg
    11461 aatatttttg cttcaa
//
